| 285 matches found in 115 documents. Search time: 0.006 seconds. |
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Score: 11.00 |
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| | Journal: J Biol Chem Citation: V : 285 P : 10454-63 | | Literature: oryza Field: abstract Doc ID: pub20118235 Accession (PMID): 20118235 | Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: Threonine 705 in the XA21 JM domain is essential for XA21 autophosphorylation in vitro and XA21-mediated innate immunity in vivo .
[ Sen. 5, subscore: 3.00 ]: The replacement of Thr ( 705 ) by an alanine or glutamic acid abolishes XA21 autophosphorylation and eliminates interactions between XA21 and four XA21-binding proteins in yeast and rice .
[ Sen. 2, subscore: 2.00 ]: The rice PRR XA21 recognizes the pathogen-associated molecular pattern , Ax21 ( activator of XA21-mediated immunity ) .
[ Sen. 3, subscore: 1.00 ]: Here we show that the XA21 juxtamembrane ( JM ) domain is required for kinase autophosphorylation .
[ Sen. 6, subscore: 1.00 ]: Although threonine residues analogous to Thr ( 705 ) of XA21 are present in the JM domains of most RD and non-RD plant receptor-like kinases , this residue is not required for autophosphorylation of the Arabidopsis RD RLK BRI1 ( brassinosteroid insensitive 1 ) .
[ Sen. 7, subscore: 1.00 ]: The threonine 705 of XA21 is conserved only in the JM domains of plant RLKs but not in those of fly , human , or mouse suggesting distinct regulatory mechanisms .
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Score: 10.00 |
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| | Journal: Mol Plant Citation: V : 3 P : 917-26 | | Literature: oryza Field: abstract Doc ID: pub20616165 Accession (PMID): 20616165 | Matching Sentences:
[ Sen. 2, subscore: 4.00 ]: Here , we showed that XA21 , its kinase-dead mutant XA21P ( K736EP ) , and the triple autophosphorylation mutant XA21P ( S686A/T688A/S699A ) GFP fusions were primarily localized to the plasma membrane ( PM ) when overexpressed in the intact transgenic rice cell , and also localized to the ER in the transgenic protoplast The transgenic plants constitutively expressing the wild-type XA21 or its GFP fusion displayed race-specific resistance to Xoo at the adult and seedling stages .
[ Sen. 3, subscore: 3.00 ]: XA21 and XA21P ( K736EP ) could be internalized probably via the SCAMP-positive early endosomal compartment in the protoplast , suggesting that XA21 might be endocytosed to initiate resistance responses during pathogen infection .
[ Sen. 1, subscore: 1.00 ]: The rice pattern recognition receptor ( PRR ) XA21 confers race-specific resistance in leaf infection by bacterial blight Xathomonas oryzae pv . oryzae ( Xoo ) , and was shown to be primarily localized to the endoplasmic reticulum ( ER ) when expressed with its native promoter or overexpressed in the protoplast However , whether the protein is still ER-localization in the intact cell when overexpressed remains to be identified .
[ Sen. 4, subscore: 1.00 ]: We also established a root infection system and demonstrated that XA21 also mediated race-specific resistance responses to Xoo in the root .
[ Sen. 5, subscore: 1.00 ]: Our current study provides an insight into the nature of the XA21-mediated resistance and a practical approach using the root cell system to further dissect the cellular signaling of the PRR during the rice-Xoo interaction .
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Score: 10.00 |
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| | Journal: Genetica Citation: V : 139 P : 1465-75 | | Literature: oryza Field: abstract Doc ID: pub22451352 Accession (PMID): 22451352 | Matching Sentences:
[ Sen. 3, subscore: 3.00 ]: Therefore , we analyzed all Xa21 gene homologs in eight whole-genome sequenced rice lines , as well as in four gramineous genomes , rice , Brachypodium , sorghum and maize ; using Arabidopsis Xa21 homologs as outgroups , 17 , 7 , 7 and 3 Xa21 homologs were detected in these four grasses , respectively .
[ Sen. 5, subscore: 2.00 ]: Within the rice species , the high level of nucleotide diversity between Xa21-like orthologs showed a strong association with the presence/absence haplotypes , suggesting that the genetic structure of rice lines plays an important role in the variations between these Xa21-like orthologs .
[ Sen. 6, subscore: 2.00 ]: Strongly positive selection was detected in the core region of the leucine-rich repeat domains of the Xa21 subclade among the rice lines , indicating that the rapid gene diversification of Xa21 homologs may be a strategy for a given species to adapt to the changing spectrum of species-specific pathogens .
[ Sen. 1, subscore: 1.00 ]: The XA21 protein has broad spectrum resistance against Xanthomonas oryzae pv . oryzae .
[ Sen. 2, subscore: 1.00 ]: Although Xa21-mediated immunity is well characterized , little is known about the origin and evolutionary history of this gene in grasses .
[ Sen. 4, subscore: 1.00 ]: Synteny and phylogenetic analysis showed that frequent gene translocation , duplication and/or loss , have occurred at Xa21 homologous loci , suggesting that they have undergone or are undergoing rapid generation of copy number variations .
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Score: 9.00 |
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| | Journal: J Biol . Chem . Citation: V : 277 ( 23 ) P : 20264-9 | | Literature: oryza Field: abstract Doc ID: pub11927577 Accession (PMID): 11927577 | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Moreover , the active XA21 kinase can not phosphorylate a kinase-deficient mutant of XA21 kinase .
[ Sen. 1, subscore: 1.00 ]: The rice disease resistance gene , Xa21 , encodes a receptor kinase-like protein consisting of leucine-rich repeats in the putative extracellular domain and a serine/threonine kinase in the putative intracellular domain .
[ Sen. 2, subscore: 1.00 ]: The putative XA21 kinase domain was expressed as maltose-binding and glutathione S-transferase fusion proteins in Escherichia coli .
[ Sen. 5, subscore: 1.00 ]: The relative phosphorylation rate of the XA21 kinase against increasing enzyme concentrations follows a first-order rather than second-order kinetics , indicating an intramolecular phosphorylation mechanism .
[ Sen. 7, subscore: 1.00 ]: The enzymatic activity of the XA21 kinase in a buffer containing Mn ( 2+ ) is at least 15 times higher than that with Mg ( 2+ ) .
[ Sen. 8, subscore: 1.00 ]: The K ( m ) and V ( max ) of XA21 kinase for ATP are 0 . 3 microm and 8 . 4 nmol/mg/min , respectively .
[ Sen. 9, subscore: 1.00 ]: Tryptic phosphopeptide mapping reveals that multiple sites on the XA21 kinase are phosphorylated .
[ Sen. 10, subscore: 1.00 ]: Finally , our data suggest that the region of XA21 kinase corresponding to the RD kinase activation domain is not phosphorylated , revealing a distinct mode of action compared with the tomato Pto serine/threonine kinase conferring disease resistance .
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Score: 9.00 |
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| | Journal: Citation: V : ( ) P : | | Literature: oryza Field: abstract Doc ID: pub17172358 Accession (PMID): 17172358 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase .
[ Sen. 3, subscore: 2.00 ]: The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays .
[ Sen. 7, subscore: 2.00 ]: These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance .
[ Sen. 1, subscore: 1.00 ]: XA21 is a receptor-like kinase protein in rice ( Oryza sativa ) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease , Xanthomonas oryzae pv oryzae .
[ Sen. 4, subscore: 1.00 ]: XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively .
[ Sen. 6, subscore: 1.00 ]: Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein .
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Score: 9.00 |
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| | Journal: PLoS Biol Citation: V : 6 P : e231 | | Literature: oryza Field: abstract Doc ID: pub18817453 Accession (PMID): 18817453 | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: A yeast two-hybrid screen using the intracellular portion of XA21 , including the juxtamembrane ( JM ) and kinase domain as bait , identified a protein phosphatase 2C ( PP2C ) , called XA21 binding protein 15 ( XB15 ) .
[ Sen. 3, subscore: 1.00 ]: The rice ( Oryza sativa L ) XA21 receptor kinase is a key recognition and signaling determinant in the innate immune response .
[ Sen. 5, subscore: 1.00 ]: The interaction of XA21 and XB15 was confirmed in vitro and in vivo by glutathione-S-transferase ( GST ) pull-down and co-immunoprecipitation assays , respectively .
[ Sen. 7, subscore: 1.00 ]: Autophosphorylated XA21 can be dephosphorylated by XB15 in a temporal and dosage-dependent manner .
[ Sen. 8, subscore: 1.00 ]: A serine residue in the XA21 JM domain is required for XB15 binding .
[ Sen. 9, subscore: 1.00 ]: Xb15 mutants display a severe cell death phenotype , induction of pathogenesis-related genes , and enhanced XA21-mediated resistance .
[ Sen. 10, subscore: 1.00 ]: Overexpression of Xb15 in an XA21 rice line compromises resistance to the bacterial pathogen Xanthomonas oryzae pv . oryzae .
[ Sen. 11, subscore: 1.00 ]: These results demonstrate that Xb15 encodes a PP2C that negatively regulates the XA21-mediated innate immune response .
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Score: 9.00 |
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| | Journal: Plant Cell Citation: V : 10 ( 5 ) P : 765-79 | | Literature: oryza Field: abstract Doc ID: pub9596635 Accession (PMID): 9596635 | Matching Sentences:
[ Sen. 2, subscore: 3.00 ]: Analysis of the inheritance patterns and resistance spectra of transgenic plants carrying six Xa21 gene family members indicated that one member , designated Xa21D , displayed a resistance spectrum identical to that observed for Xa21 but conferred only partial resistance .
[ Sen. 5, subscore: 2.00 ]: Comparison of nucleotide substitutions in the LRR coding regions of Xa21 and Xa21D provided evidence of adaptive selection .
[ Sen. 1, subscore: 1.00 ]: The rice Xa21 gene confers resistance to Xanthomonas oryzae pv oryzae in a race-specific manner .
[ Sen. 3, subscore: 1.00 ]: Xa21D encodes a receptor-like protein carrying leucine-rich repeat ( LRR ) motifs in the presumed extracellular domain .
[ Sen. 4, subscore: 1.00 ]: The Xa21D transcript terminates shortly after the stop codon introduced by the retrotransposon Retrofit .
[ Sen. 6, subscore: 1.00 ]: Both functional and evolutionary evidence indicates that the Xa21D LRR domain controls race-specific pathogen recognition .
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Score: 8.00 |
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| | Journal: Mol . Gen . Genet . Citation: V : 236 ( 1 ) P : 113-20 | | Literature: oryza Field: abstract Doc ID: pub1362973 Accession (PMID): 1362973 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: One chromosome 11 marker ( RG103 ) detected polymorphism between the NILs that cosegregated with Xa21 .
[ Sen. 3, subscore: 1.00 ]: All other chromosome 11 DNA markers tested were monomorphic between the NILs , localizing the Xa21 introgressed region to an 8 . 3 cM interval on chromosome 11 .
[ Sen. 5, subscore: 1.00 ]: Genomic sequences hybridizing with RAPD818 , RAPD248 and RG103 were duplicated specifically in the Xa21 NIL .
[ Sen. 6, subscore: 1.00 ]: All three markers cosegregated with the resistance locus , Xa21 , in a F2 population of 386 progeny .
[ Sen. 7, subscore: 1.00 ]: Based on the frequency with which we recovered polymorphic Xa21-linked markers , we estimated the physical size of the introgressed region to be approximately 800 kb .
[ Sen. 8, subscore: 1.00 ]: This estimation was supported by physical mapping ( using pulsed field gel electrophoresis ) of the sequences hybridizing with the three Xa21-linked DNA markers .
[ Sen. 9, subscore: 1.00 ]: The results showed that the three Xa21-linked markers are physically close to each other , with one copy of the RAPD818 sequences located within 60 kb of RAPD248 and the other copy within 270 kb of RG103 .
[ Sen. 10, subscore: 1.00 ]: None of the enzymes tested generated a DNA fragment that hybridized with all three of the markers indicating that the introgressed region containing the resistance locus Xa21 is probably larger than 270 kb .
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Score: 8.00 |
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| | Journal: Theor . Appl . Genet . Citation: V : 108 ( 3 ) P : 379-84 | | Literature: oryza Field: abstract Doc ID: pub14523518 Accession (PMID): 14523518 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: To identify genes essential for the function of the Xa21 gene , 4 , 500 IRBB21 ( Xa21 isogenic line in IR24 background ) mutants , induced by diepoxybutane and fast neutrons , were screened against Philippine race six ( PR6 ) Xoo for a change from resistance to susceptibility .
[ Sen. 1, subscore: 1.00 ]: The rice gene , Xa21 , confers resistance to diverse races of Xanthomonas oryzae pv . oryzae ( Xoo ) and encodes a receptor-like kinase with leucine-rich repeats in the extra-cellular domain .
[ Sen. 4, subscore: 1.00 ]: All fully susceptible mutants carried rearrangements at the Xa21 locus as detected by PCR and Southern hybridization .
[ Sen. 5, subscore: 1.00 ]: For the partially susceptible mutants , no changes were detected at the Xa21 locus based on Southern and PCR analyses .
[ Sen. 6, subscore: 1.00 ]: However , two of these mutants were confirmed via genetic analysis to have mutations at the Xa21 locus .
[ Sen. 7, subscore: 1.00 ]: Partially susceptible mutants exhibited variation in level of susceptibility to different Xoo strains , suggesting that they may carry different mutations required for the Xa21-mediated resistance .
[ Sen. 8, subscore: 1.00 ]: The mutants identified in this study provide useful materials for dissecting the Xa21-mediated resistance pathway in rice .
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Score: 8.00 |
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| | Journal: Theor . Appl . Genet . Citation: V : 109 ( 3 ) P : 534-42 | | Literature: oryza Field: abstract Doc ID: pub15088086 Accession (PMID): 15088086 | Matching Sentences:
[ Sen. 10, subscore: 2.00 ]: On the basis of genetic mapping of the T-DNA Xa21 loci , the BB resistance effects of the transgene Xa21 at different chromosome locations were investigated using homozygous transgenic lines with only one copy of the transgene .
[ Sen. 1, subscore: 1.00 ]: The genetic loci and phenotypic effects of the transgene Xa21 , a bacterial blight ( BB ) resistance gene cloned from rice , were investigated in transgenic rice produced through an Agrobacterium-mediated transformation system .
[ Sen. 2, subscore: 1.00 ]: The flanking sequences of integrated T-DNAs were isolated from Xa21 transgenic rice lines using thermal asymmetric interlaced PCR .
[ Sen. 3, subscore: 1.00 ]: Based on the analysis of 24 T-DNA Xa21 flanking sequences , T-DNA loci in rice could be classified into three types : the typical T-DNA integration with the definite left and right borders , the T-DNA integration linked with the adjacent vector backbone sequences and the T-DNA integration involved in a complicated recombination in the flanking sequences .
[ Sen. 5, subscore: 1.00 ]: All three types of integrated transgene Xa21 could be stably inherited and expressed the BB resistance through derived generations in their respective transgenic lines .
[ Sen. 11, subscore: 1.00 ]: Among the transgenic lines , no obvious position effects of the transgene Xa21 were observed .
[ Sen. 12, subscore: 1.00 ]: In addition , the BB resistance levels of the Xa21 transgenic plants with different transgene copy numbers and on different genetic backgrounds were also investigated .
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Score: 8.00 |
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| | Journal: Cell Microbiol Citation: V : 12 P : 1017-25 | | Literature: oryza Field: abstract Doc ID: pub20590657 Accession (PMID): 20590657 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: In 1995 , we isolated the gene controlling this resistance and in 2009 demonstrated that XA21 recognizes a highly conserved peptide , called Ax21 ( activator of XA21-mediated immunity ) .
[ Sen. 6, subscore: 2.00 ]: Because XA21 is representative of the large number of predicted pattern recognition receptors ( PRRs ) in rice ( n = 328 ) , Arabidopsis ( n = 35 ) and other plant species , further characterization of XA21-mediated signalling pathways will contribute to elucidation of these important defence responses .
[ Sen. 1, subscore: 1.00 ]: In the early 1970s , the Xa21 gene from the wild rice species Oryza longistaminata drew attention of rice breeders because of its broad-spectrum resistance to diverse strains of a serious bacterial disease of rice in Asia and Africa , called bacterial blight disease , caused by the Gram-negative bacterium , Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 3, subscore: 1.00 ]: Tyrosine sulfation of Ax21 is required for recognition by rice XA21 .
[ Sen. 4, subscore: 1.00 ]: A decade of genetic , molecular and biochemical studies have uncovered key components of the XA21-mediated signalling cascade .
[ Sen. 5, subscore: 1.00 ]: Ax21 recognition by XA21 at the cell surface induces phosphorylation-mediated events , which are predicted to alter subcellular localization and/or DNA-binding activity of a WRKY family of transcription factors .
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Score: 7.00 |
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| | Journal: Plant J Citation: V : 20 ( 2 ) P : 231-6 | | Literature: oryza Field: abstract Doc ID: pub10571882 Accession (PMID): 10571882 | Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: Expression of the Xa21 gene transcript is not correlated with expression of Xa21 disease resistance indicating that the developmental regulation of Xa21-resistance is either controlled post-transcriptionally or by other factors .
[ Sen. 1, subscore: 1.00 ]: The rice resistance gene Xa21 confers resistance against the bacterial pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 2, subscore: 1.00 ]: The molecular genetic mechanism controlling the integration of the Xa21-mediated disease resistance response with the developmental program in rice is under study in this model system .
[ Sen. 4, subscore: 1.00 ]: Xa21-resistance progressively increases from the susceptible juvenile leaf 2 stage through later stages , with 100% resistance at the adult leaf 9/10 stage .
[ Sen. 5, subscore: 1.00 ]: We found that Xa21 expression is independent of plant developmental stage , infection with Xoo , or wounding .
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Score: 7.00 |
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| | Journal: Plant J Citation: V : 45 ( 5 ) P : 740-51 | | Literature: oryza Field: abstract Doc ID: pub16460508 Accession (PMID): 16460508 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: Xa21 encodes a receptor-like kinase ( XA21 ) .
[ Sen. 1, subscore: 1.00 ]: The rice gene Xa21 confers resistance against Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 3, subscore: 1.00 ]: We demonstrate that XA21 autophosphorylates residues Ser686 , Thr688 and Ser689 in vitro .
[ Sen. 5, subscore: 1.00 ]: Plants carrying these same substitutions in XA21 were compromised in their resistance to the normally avirulent Xoo Philippine race 6 .
[ Sen. 6, subscore: 1.00 ]: Additionally , we show that wild-type XA21 and the kinase-dead mutant with the invariable Lys736 residue mutated to glutamic acid were also proteolytically degraded in protein extracts .
[ Sen. 8, subscore: 1.00 ]: We propose that autophosphorylation of Ser686 , Thr688 and Ser689 functions to stabilize XA21 against the developmentally controlled proteolytic activity .
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Score: 7.00 |
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| | Journal: Plant Sci Citation: V : 179 P : 466-71 | | Literature: oryza Field: abstract Doc ID: pub21076626 Accession (PMID): 21076626 | Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: Here we report that Xa21 is induced by Xoo infection and that ectopic expression of Xa21 confers resistance at three leaf stage ( three-week-old ) , overcoming the developmental limitation of XA21-mediated resistance .
[ Sen. 5, subscore: 2.00 ]: Ectopic expression of Xa21 also up-regulates a larger set of defense-related genes as compared to Xa21 driven by the native promoter .
[ Sen. 2, subscore: 1.00 ]: The rice PRR , XA21 , confers robust resistance at adult stages to most strains of the bacterial pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 6, subscore: 1.00 ]: These results indicate that altered regulation of Xa21 expression is useful for developing enhanced resistance to Xoo at multiple developmental stages .
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Score: 6.00 |
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| | Journal: Sheng Wu Gong Cheng Xue Bao Citation: V : 19 ( 2 ) P : 153-7 | | Literature: oryza Field: abstract Doc ID: pub15966313 Accession (PMID): 15966313 | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: The objective of this study is to improve the BB resistance of Shuhui527 by introgressing Xa21 and Xa4 , the two broad-spectrum BB resistance genes , into Shuhui527 with IRBB60 as the donor , pTA248 and MP12 , linking tightly with Xa21 and Xa4 respectively as DNA markers .
[ Sen. 3, subscore: 1.00 ]: The IRBB60 , from the International Rice Research Institute ( IRRI ) , contains dominant genes Xa21 and Xa4 conferring resistance to BB .
[ Sen. 5, subscore: 1.00 ]: BC1 F1 progenies of ( Shuhui527 x IRBB60 ) , containing Xa21 and Xa4 identified using PCR screening and with agronomic traits including plant type , grain type and days to heading etc similar to those of Shuhui527 , were subsequently backcrossed to Shuhui527 and self-pollinated to generate BC2 F1 and BC1 F2 . The BC3 F1 and BC3 F2 were subsequently developed using the same approach .
[ Sen. 6, subscore: 1.00 ]: Among the 20 BC3 F2 plants , homozygous Xa21 and Xa4 , 10 plants were the most similar to Shuhui527 in the agronomic traits , and were screened using 120 pairs SSR and 100 pairs RAPD markers .
[ Sen. 9, subscore: 1.00 ]: The evaluation of the polymorphisms and selection accuracies of pTA248 and MP12 demonstrated that the polymorphisms of the two markers were obvious and co-dominant and the accuracies were more than 97% and 83% respectively , indicating the two markers are good for Xa21 and Xa4 in Molecular Marker-assisted Selection .
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Score: 6.00 |
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| | Journal: Ann Bot ( Lond ) Citation: V : 100 P : 205-17 | | Literature: oryza Field: abstract Doc ID: pub17576658 Accession (PMID): 17576658 | Matching Sentences:
[ Sen. 12, subscore: 2.00 ]: The other clone encoded an Xa21-like protein , Xa21G .
[ Sen. 15, subscore: 2.00 ]: When infected with X oryzae pv . oryzae , against which Xa21 confers resistance in a gene-for-gene manner , the progeny of Line-2 were apparently resistant while the wild type was highly susceptible without Xa21G expression .
[ Sen. 14, subscore: 1.00 ]: Expression of Xa21G was not detectable in wild type but was constitutive in Line-2 .
[ Sen. 16, subscore: 1.00 ]: CONCLUSIONS : These results indicated that demethylation was selective in Line-2 , and that promoter demethylation abolished the constitutive silencing of Xa21G due to hypermethylation , resulting in acquisition of disease resistance .
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Score: 6.00 |
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| | Journal: Wei Sheng Wu Xue Bao Citation: V : 47 P : 1009-12 | | Literature: oryza Field: abstract Doc ID: pub18271255 Accession (PMID): 18271255 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: Xa21 confers resistance to bacterial blight , while Pi-d2 confers resistance to rice blast Both Xa21 and Pi-d2 encode receptor kinase-like proteins .
[ Sen. 3, subscore: 1.00 ]: Biochemical properties of XA21 kinase expressed in bacterial were characterized in our previous report .
[ Sen. 4, subscore: 1.00 ]: In this study , both XA21 and PI-D2 kinase domain were PCR amplified and cloned into yeast expression vector pEGH via recombinational cloning strategy , kinase proteins expressed in eukaryotic yeast system was purified and autophosphorylation assay was carried out .
[ Sen. 5, subscore: 1.00 ]: The results indicated that XA21 and PI-D2 protein can be detected by SDS-PAGE and showed expected molecular weight .
[ Sen. 6, subscore: 1.00 ]: Autophosphorylation assay indicated that yeast expressed XA21 and PI-D2 were active when incubated with P32 labelled ATP .
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Score: 6.00 |
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| | Journal: Theor Appl Genet Citation: V : P : | | Literature: oryza Field: abstract Doc ID: pub19390838 Accession (PMID): 19390838 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Xa3/Xa26 and Xa21 , encoding leucine-rich repeat ( LRR ) -kinase type plasma membrane proteins , mediate race-specific resistance to Xanthomonas oryzae pv . oryzae ( Xoo ) , which causes bacterial blight , one of the most devastating rice diseases .
[ Sen. 3, subscore: 1.00 ]: Plants carrying Xa3/Xa26 and plants carrying Xa21 have different resistance spectra and the functions of the two R genes are regulated by developmental stage .
[ Sen. 4, subscore: 1.00 ]: Four chimeric genes encoding proteins consisting of different parts of XA3/XA26 and XA21 were constructed by domain swapping and transformed into a susceptible rice variety .
[ Sen. 5, subscore: 1.00 ]: The resistance spectra and development-regulated resistance of the transgenic plants carrying Xa3/Xa26 , Xa21 , or chimeric gene to different Xoo strains were analyzed in the same genetic background .
[ Sen. 6, subscore: 1.00 ]: The results suggest that the gradually increased expression of Xa3/Xa26 and Xa21 plays an important role in the progressively enhanced Xoo resistance during rice development .
[ Sen. 7, subscore: 1.00 ]: In addition , the LRR domains of XA3/XA26 and XA21 are important determinants of race-specific recognition during rice-Xoo interaction , but juxtamembrane regions of the two R proteins also appear to contribute to resistance specificity .
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Score: 6.00 |
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| | Journal: PLoS One Citation: V : 5 P : e9262 | | Literature: oryza Field: abstract Doc ID: pub20174657 Accession (PMID): 20174657 | Matching Sentences:
[ Sen. 5, subscore: 3.00 ]: In BiP3-overexpressing rice plants , XA21-mediated immunity is compromised , XA21 stability is significantly decreased , and XA21 proteolytic cleavage is inhibited .
[ Sen. 3, subscore: 1.00 ]: Here we report the in vivo interaction of the endoplasmic reticulum ( ER ) chaperone BiP3 with the rice XA21 PRR , which confers resistance to the Gram negative bacterium , Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 4, subscore: 1.00 ]: We show that XA21 is glycosylated and is primarily localized to the ER and also to the plasma membrane ( PM ) .
[ Sen. 7, subscore: 1.00 ]: These results indicate that BiP3 regulates XA21 protein stability and processing and that this regulation is critical for resistance to Xoo .
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Score: 5.00 |
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| | Journal: Sheng Wu Gong Cheng Xue Bao Citation: V : 22 ( 2 ) P : 204-10 | | Literature: oryza Field: abstract Doc ID: pub16607944 Accession (PMID): 16607944 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The dominant gene Xa21 with broad-spectrum and high resistance to Xanthomonas oryzae pv . oryzae ( Xoo ) was transferred into C418 , an important restorer line of japonica hybrid rice in China using double right-border ( DRB ) T-DNA binary vector through Agrobacterium-mediated transformation .
[ Sen. 2, subscore: 1.00 ]: 17 transgenic lines were Xa21-positive with high resistance to the race P6 of Xoo through PCR analysis and resistance identification , among the total 27 independent primary transformants ( T0 ) obtained .
[ Sen. 3, subscore: 1.00 ]: The subsequent analysis of the T1 progenies of these 17 T0 lines through PCR-assisted selection and resistance investigation showed that four Xa21 transgenic T0 lines could produce selectable marker-free ( SMF ) progenies .
[ Sen. 5, subscore: 1.00 ]: In addition , PCR analysis also revealed these SMF progenies did not contain vector backbone sequence , and they were named as SMF and vector backbone sequence-free ( SMF-VBSF ) Xa21 transgenic plants .
[ Sen. 6, subscore: 1.00 ]: The further molecular and phenotypic analysis of the T2 and T3 progenies testified the homozygous SMF-VBSF Xa21 transgenic plants were obtained with high resistance to Xoo .
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Score: 5.00 |
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| | Journal: Yi Chuan Citation: V : 28 ( 6 ) P : 745-53 | | Literature: oryza Field: abstract Doc ID: pub16818441 Accession (PMID): 16818441 | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: The molecular mechanism of resistance and its breeding application , including biochemical properties of the kinase domain of XA21 , AvrXa21 identification , the disease resistance pathway mediated by Xa21 , and the mechanism of disease resistance were summarized .
[ Sen. 1, subscore: 1.00 ]: Gene Xa21 is the first disease resistance gene cloned from rice ( Oryza sativa L ) , which encodes a receptor-like kinase and confers broad spectrum resistance against Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 2, subscore: 1.00 ]: In this paper , the discovery , mapping and cloning of Xa21 were reviewed briefly .
[ Sen. 4, subscore: 1.00 ]: Future perspective on Xa21-related research is also discussed .
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Score: 5.00 |
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| | Journal: Proc Natl Acad Sci U S A Citation: V : 107 P : 8029-34 | | Literature: oryza Field: abstract Doc ID: pub20385831 Accession (PMID): 20385831 | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Here we show that the rice PRR , XA21 , interacts with XA21 binding protein 24 ( XB24 ) , a previously undescribed ATPase .
[ Sen. 5, subscore: 1.00 ]: XB24 promotes autophosphorylation of XA21 through its ATPase activity .
[ Sen. 6, subscore: 1.00 ]: Rice lines silenced for Xb24 display enhanced XA21-mediated immunity , whereas rice lines overexpressing XB24 are compromised for immunity .
[ Sen. 8, subscore: 1.00 ]: XA21 is degraded in the presence of the pathogen-associated molecular pattern Ax21 when XB24 is overexpressed .
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Score: 5.00 |
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| | Journal: J Integr Plant Biol Citation: V : 53 P : 300-11 | | Literature: oryza Field: abstract Doc ID: pub21324061 Accession (PMID): 21324061 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The cloned rice gene Xa21 confers resistance to a broad spectrum of Xoo races .
[ Sen. 3, subscore: 1.00 ]: To identify genes involved in Xa21-mediated immunity , a whole-genome oligonucleotide microarray of rice was used to profile the expression of rice genes between incompatible interactions and mock treatments at 0 , 4 , 8 , 24 , 72 and 120 h post inoculation ( hpi ) or between incompatible and compatible interactions at 4 hpi , respectively .
[ Sen. 4, subscore: 1.00 ]: A total of 441 differentially expressed genes , designated as XDGs ( Xa21 mediated differentially expressed genes ) , were identified .
[ Sen. 7, subscore: 1.00 ]: Interestingly , most signaling and transcriptional regulator genes were downregulated at 4 and 8 hpi , suggesting that negative regulation of cellular signaling may play a role in the Xa21-mediated defense response .
[ Sen. 8, subscore: 1.00 ]: Comparison of expression profiles between Xa21 and other R gene-mediated defense systems revealed interesting common responses .
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Score: 5.00 |
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| | Journal: Nat Commun Citation: V : 3 P : 920 | | Literature: oryza Field: abstract Doc ID: pub22735448 Accession (PMID): 22735448 | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: In vivo cleavage of XA21 and translocalization of the intracellular kinase domain to the nucleus is required for the XA21-mediated immune response .
[ Sen. 2, subscore: 1.00 ]: The rice XA21 receptor confers broad-spectrum immunity to the Gram-negative bacterial pathogen , Xanthomonas oryzae pv . oryzae upon recognition of a small protein , Ax21 , that is conserved in all Xanthomonas species and related genera .
[ Sen. 3, subscore: 1.00 ]: Here we demonstrate that XA21 is cleaved to release the intracellular kinase domain and that this intracellular domain carries a functional nuclear localization sequence .
[ Sen. 4, subscore: 1.00 ]: Bimolecular fluorescence complementation assays indicate that the XA21 intracellular domain interacts with the OsWRKY62 transcriptional regulator exclusively in the nucleus of rice protoplasts .
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Score: 4.00 |
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| | Journal: Yi Chuan Xue Bao Citation: V : 29 ( 10 ) P : 880-6 | | Literature: oryza Field: abstract Doc ID: pub12561472 Accession (PMID): 12561472 | Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: The genetic mapping of T-DNA integration sites in Xa21 transgenic rice will benefit the study of position effect and stable inheritance of the transgene Xa21 .
[ Sen. 2, subscore: 1.00 ]: In the previous research we have transferred the Xa21 gene into five rice varieties of China , using Agrobacterium-mediated trasformation .
[ Sen. 3, subscore: 1.00 ]: In this study , T-DNA flanking sequences of Xa21 transgenic rice lines were obtained by using thermal asymmetric interlaced PCR ( TAIL-PCR ) .
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Score: 4.00 |
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| | Journal: Theor . Appl . Genet . Citation: V : 108 ( 1 ) P : 53-61 | | Literature: oryza Field: abstract Doc ID: pub13679986 Accession (PMID): 13679986 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice gene Xa21 represents a unique class of plant disease resistance ( R ) genes with distinct protein structure and broad-spectrum specificity ; few sequences or genes of this class have been cloned and characterized in other plant species .
[ Sen. 2, subscore: 1.00 ]: Degenerate primers were designed from the conserved motifs in the kinase domains of Xa21 and tomato Pto , and used in PCR amplification to identify this class of resistance gene candidate ( RGC ) sequences from citrus for future evaluation of possible association with citrus canker resistance .
[ Sen. 3, subscore: 1.00 ]: Twenty-nine RGC sequences highly similar to the kinase domain of Xa21 ( 55%-60% amino-acid identity ) were cloned and characterized .
[ Sen. 8, subscore: 1.00 ]: A primer-walking strategy was employed to derive full-length gene structures from two BAC clones ; both sequences 17o6RLK and 26m19RLK contained all the features of the rice Xa21 protein , including a signal peptide , the same number of leucine-rich-repeats , and transmembrane and kinase domains .
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Score: 4.00 |
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| | Journal: Yi Chuan Citation: V : 27 ( 3 ) P : 382-286 | | Literature: oryza Field: abstract Doc ID: pub15985401 Accession (PMID): 15985401 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The Xa21 gene previously cloned from the wild rice species Oryzae longistaminata confers broad-spectrum resistance to rice leaf blight caused by different strains of Xanthomonas oryzae pv . oryzae .
[ Sen. 2, subscore: 1.00 ]: Here we attempted to determine the existence of Xa21 homologs in other wild rice species and rice cultivars and the sequence differences between the homologs .
[ Sen. 3, subscore: 1.00 ]: We synthesized specific primers based on the reported Xa21 sequence to amplify homologs of the gene exon II from several rice cultivars and three wild rice species in Yunnan Province , China .
[ Sen. 4, subscore: 1.00 ]: The fragments cloned from various types of O rufipogon Griff from Jinghong and Yuanjiang , Yunnan Province , were highly homologous to the reported Xa21 gene exon II .
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Score: 4.00 |
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| | Journal: Sheng Wu Gong Cheng Xue Bao Citation: V : 25 P : 605-10 | | Literature: oryza Field: abstract Doc ID: pub19637639 Accession (PMID): 19637639 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: The competitive template of Xa21 was the endogenous Xa2l homologous sequence located on chromosome 11 .
[ Sen. 6, subscore: 1.00 ]: Through competitive PCR analysis of transgenic T1 plants produced by double right border binary vector , false positive or false negative samples were effectively diminished , and genuine SMF Xa21 transgenic plants were obviously obtained .
[ Sen. 7, subscore: 1.00 ]: Comparing with the conventional non-competitive PCR , competitive PCR increased the accuracy for selecting SMF Xa21 transgenic plants .
[ Sen. 8, subscore: 1.00 ]: The results of bacterial blight ( BB ) resistance tests and hygromycin B resistance assay of SMF Xa21 transgenic plants testified the reliability of this method .
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Score: 4.00 |
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| | Journal: Science Citation: V : 326 P : 850-3 | | Literature: oryza Field: abstract Doc ID: pub19892983 Accession (PMID): 19892983 | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Using coimmunoprecipitation , we found that XA21 is required for axY ( S ) 22 binding and recognition . axY ( S ) 22 is 100% conserved in all analyzed Xanthomonas species , confirming that Ax21 is a pathogen-associated molecular pattern and that XA21 is a pattern recognition receptor .
[ Sen. 1, subscore: 1.00 ]: The rice Xa21 gene confers immunity to most strains of the bacterium Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 2, subscore: 1.00 ]: Liquid chromatography-tandem mass spectrometry analysis of biologically active fractions from Xoo supernatants led to the identification of a 194-amino acid protein designated Ax21 ( activator of XA21-mediated immunity ) .
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Score: 4.00 |
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| | Journal: Metabolomics Citation: V : 6 P : 451-465 | | Literature: oryza Field: abstract Doc ID: pub20676379 Accession (PMID): 20676379 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: A transgenic variety , TP309_Xa21 , expresses the pattern recognition receptor Xa21 , and is resistant .
[ Sen. 5, subscore: 1.00 ]: PXO99 big up tri , openraxST , a strain lacking the raxST gene , is able to overcome Xa21-mediated immunity .
[ Sen. 11, subscore: 1.00 ]: Although few mRNA and metabolite differences were detected in PXO99 challenged TP309 compared to mock , many differential changes occurred in the Xa21-mediated response to PXO99 and PXO99 big up tri , openraxST .
[ Sen. 13, subscore: 1.00 ]: Significant transcriptional induction of several pathogenesis related genes in Xa21 challenged strains , as well as differential changes to GAD , PAL , ICL1 and Glutathione-S-transferase transcripts indicated limited correlation with metabolite changes under single time point global profiling conditions .
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Score: 4.00 |
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| | Journal: Yi Chuan Xue Bao Citation: V : 23 ( 2 ) P : 110-6 | | Literature: oryza Field: abstract Doc ID: pub8695180 Accession (PMID): 8695180 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Xa21 is a newly found gene of rice which confers resistance to bacterial blight caused by Xanthomonas oryzae pv . oryzae .
[ Sen. 4, subscore: 1.00 ]: We produced two F2 populations between one resistant line IRBB21 containing Xa21 and two susceptible varieties , respectively .
[ Sen. 6, subscore: 1.00 ]: Cosegregation between Xa21 and PB78 was studied in the two F2 populations .
[ Sen. 7, subscore: 1.00 ]: The results showed that Xa21 was closely linked to the molecular marker , the crossing over value was 2 . 48% .
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Score: 3.00 |
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| | Journal: Sheng Wu Gong Cheng Xue Bao Citation: V : 16 ( 2 ) P : 137-41 | | Literature: oryza Field: abstract Doc ID: pub10976312 Accession (PMID): 10976312 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Agrobacterium-mediated transformation of two-line genic male sterile Indica rice variety Peiai 64S was conducted using a cloned gene , Xa21 , as the foreign gene and mature embryo calli as the recipients .
[ Sen. 3, subscore: 1.00 ]: The PCR analysis and Southern blotting showed the integration of Xa21 gene into the genome the transgenic plants .
[ Sen. 5, subscore: 1.00 ]: Analyses of T1 plants of the tested transgenic lines showed that integrated Xa21 gene could be steadily inherited and segregated in a 3 : 1 ratio .
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Score: 3.00 |
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| | Journal: Genetics Citation: V : 159 ( 2 ) P : 757-65 | | Literature: oryza Field: abstract Doc ID: pub11606550 Accession (PMID): 11606550 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The resistance of rice to its bacterial blight pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) has both qualitative and quantitative components that were investigated using three near-isogenic line sets for four resistance ( R ) genes ( Xa4 , xa5 , xa13 , and Xa21 ) and 12 Xoo races .
[ Sen. 6, subscore: 1.00 ]: Two R genes , Xa4 and Xa21 , showed complete dominance against the avirulent Xoo races and had large residual effects against virulent ones .
[ Sen. 10, subscore: 1.00 ]: There was a strong interaction leading to increased resistance between xa13 and xa5 and between either of them and Xa4 or Xa21 , suggesting their regulatory roles in the rice defensive pathway ( s ) .
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Score: 3.00 |
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| | Journal: Sheng Wu Gong Cheng Xue Bao Citation: V : 17 ( 4 ) P : 380-4 | | Literature: oryza Field: abstract Doc ID: pub11702691 Accession (PMID): 11702691 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The cloned bacterial blight ( BB ) resistance gene Xa21 was transferred into C418 , a major restorer line of japonica hybrid rice in China , using an Agrobacterium-mediated system .
[ Sen. 4, subscore: 1.00 ]: The selected transgenic restorer lines were then crossed with a commonly used sterile line , TijinA , to produce Xa21 transgenic hybrid rice .
[ Sen. 8, subscore: 1.00 ]: The propagation of this BB resistant hybrid variety with the transgene Xa21 with extend hybrid rice production in north China .
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Score: 3.00 |
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| | Journal: Mol . Microbiol . Citation: V : 44 ( 1 ) P : 37-48 | | Literature: oryza Field: abstract Doc ID: pub11967067 Accession (PMID): 11967067 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: Xanthomonas oryzae pv . oryzae ( Xoo ) Philippine race 6 ( PR6 ) is unable to cause bacterial blight disease on rice lines containing the rice resistance gene Xa21 but is virulent on non-Xa21 rice lines , indicating that PR6 carries avirulence ( avrXa21 ) determinants required for recognition by XA21 .
[ Sen. 4, subscore: 1.00 ]: Xoo PR6 strains carrying disruptions in either gene , PR6DeltaraxP or PR6DeltaraxQ , are unable to produce APS and PAPS and are virulent on Xa21-containing rice lines .
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Score: 3.00 |
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| | Journal: Mol .
Plant Microbe Interact .
Citation: V : 17 ( 6 ) P : 593-601 | | Literature: oryza Field: abstract Doc ID: pub15195942 Accession (PMID): 15195942 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The rice Xa21 gene encodes a receptor-like kinase that provides immunity against strains of the bacterial pathogen Xanthomonas oryzae pv . oryzae carrying AvrXa21 activity .
[ Sen. 6, subscore: 1.00 ]: Sequence analysis of three naturally occurring X oryzae pv . oryzae strains no longer recognized by Xa21 revealed alterations in the raxST and raxA genes .
[ Sen. 8, subscore: 1.00 ]: These results indicate that bacterial type I secretion is necessary for Xa21-mediated recognition and immunity and further suggest that type I secretion and modification of pathogen-associated molecules play an important role in triggering the innate immune response in rice .
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Score: 3.00 |
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| | Journal: Mol .
Plant Microbe Interact .
Citation: V : 17 ( 6 ) P : 602-12 | | Literature: oryza Field: abstract Doc ID: pub15195943 Accession (PMID): 15195943 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: X oryzae pv . oryzae Philippine race 6 ( PR6 ) strains are unable to establish infection in rice lines expressing the resistance gene Xa21 .
[ Sen. 3, subscore: 1.00 ]: Although the pathogen-associated molecule that triggers the Xa21-mediated defense response ( AvrXa21 ) is unknown , six rax ( required for AvrXa21 activity ) genes encoding proteins involved in sulfur metabolism and Type I secretion were recently identified .
[ Sen. 5, subscore: 1.00 ]: Null mutants of PR6 strain PXO99 that are impaired in either raxR , raxH , or both cause lesions significantly longer and grow to significantly higher levels than does the wild-type strain in Xa21-rice leaves .
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Score: 3.00 |
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| | Journal: Yi Chuan Xue Bao Citation: V : 31 ( 12 ) P : 1381-7 | | Literature: oryza Field: abstract Doc ID: pub15633644 Accession (PMID): 15633644 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice calli derived from anther culture were used as recipient to transfer a rice blight resistance gene , Xa21 , into a japonica rice variety , Taipei 309 , via Agrobacterium-mediated transformation .
[ Sen. 3, subscore: 1.00 ]: PCR , Southern blot , FISH and blight resistance analysis all indicated that Xa21 gene has been integrated into the T0 plant genomes .
[ Sen. 5, subscore: 1.00 ]: Chi2 test showed that two T1 populations segregated with a ratio of 3 : 1 , indicating that a single copy of Xa21 gene was integrated into the genome , whereas the segregation ratios of the other two T1 populations were non-Mendelian .
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Score: 3.00 |
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| | Journal: Plant J Citation: V : 43 ( 5 ) P : 623-35 | | Literature: oryza Field: abstract Doc ID: pub16115061 Accession (PMID): 16115061 | Matching Sentences:
[ Sen. 12, subscore: 2.00 ]: The fact that NRR compromises Xa21-mediated resistance indicates cross-talk or overlap between NH1 and Xa21-mediated pathways .
[ Sen. 8, subscore: 1.00 ]: When constitutively over-expressed in rice , NRR affected basal resistance , age-related resistance and Xa21-mediated resistance , causing enhanced susceptibility to Xoo .
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Score: 3.00 |
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| | Journal: Plant J Citation: V : 64 P : 343-54 | | Literature: oryza Field: abstract Doc ID: pub21070413 Accession (PMID): 21070413 | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: The expression of either CRXa1 or CRXa3 , each of which contains the whole extracellular portion of CEBiP , the whole intracellular domain of XA21 , and the transmembrane domain from either CEBiP or XA21 , induced cell death accompanied by an increased production of reactive oxygen and nitrogen species after treatment with CE .
[ Sen. 5, subscore: 1.00 ]: To enhance defense responses to CE , we constructed chimeric genes composed of CEBiP and Xa21 , which mediate resistance to rice bacterial leaf blight .
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Score: 3.00 |
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| | Journal: Plant Signal Behav Citation: V : 6 P : 449-51 | | Literature: oryza Field: abstract Doc ID: pub21364321 Accession (PMID): 21364321 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: CEBiP is a receptor for the chitin elicitor ( CE ) identified in the rice plasma membrane and XA21 is a member of the receptor-like protein kinase ( RLK ) family that confers disease resistance to rice bacterial leaf blight expressing the sulfated protein Ax21 .
[ Sen. 4, subscore: 1.00 ]: To improve resistance to rice blast , the most serious fungal disease of rice , we aimed to create a defense system that combines high affinity of CEBiP for CE and the ability of XA21 to confer disease resistance .
[ Sen. 5, subscore: 1.00 ]: Cultured rice cells expressing the chimeric receptor CRXA , which consists of CEBiP and the intracellular region of XA21 , induced cell death accompanied by an increased production of reactive oxygen and nitrogen species after exposure to CE .
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Score: 3.00 |
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| | Journal: Plant Biol ( Stuttg ) Citation: V : P : | | Literature: oryza Field: abstract Doc ID: pub22672582 Accession (PMID): 22672582 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice Xa21 gene encodes a receptor-like kinase that confers broad-spectrum resistance against Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 2, subscore: 1.00 ]: Recently , a number of genes involved in the Xa21-mediated disease resistance pathway have been identified .
[ Sen. 4, subscore: 1.00 ]: Using Western blotting , we systematically investigated the expression profile of the proteins in Xa21-mediated disease resistance response .
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Score: 3.00 |
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| | Journal: Science Citation: V : 270 ( 5243 ) P : 1804-6 | | Literature: oryza Field: abstract Doc ID: pub8525370 Accession (PMID): 8525370 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice Xa21 gene , which confers resistance to Xanthomonas oryzae pv . oryzae race 6 , was isolated by positional cloning .
[ Sen. 2, subscore: 1.00 ]: Fifty transgenic rice plants carrying the cloned Xa21 gene display high levels of resistance to the pathogen .
[ Sen. 4, subscore: 1.00 ]: Characterization of Xa21 should facilitate understanding of plant disease resistance and lead to engineered resistance in rice .
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Score: 3.00 |
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| | Journal: Plant Mol . Biol . Citation: V : 35 ( 1-2 ) P : 179-86 | | Literature: oryza Field: abstract Doc ID: pub9291971 Accession (PMID): 9291971 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice gene Xa21 conferring resistance to Xanthomonas oryzae pv . oryzae ( Xoo ) , was isolated using a map-based cloning strategy .
[ Sen. 2, subscore: 1.00 ]: Compared with previously cloned genes , the structure of Xa21 represents a novel class of plant disease R genes encoding a putative receptor kinase ( RK ) .
[ Sen. 3, subscore: 1.00 ]: This article proposes a model for the mode of action of Xa21 and summarizes our current knowledge of the modular basis of resistance in rice to bacterial leaf blight and blast
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Score: 2.00 |
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| | Journal: Yi Chuan Xue Bao Citation: V : 28 ( 4 ) P : 345-51 | | Literature: oryza Field: abstract Doc ID: pub11329876 Accession (PMID): 11329876 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Using thermal asymmetric interlaced PCR ( TAIL-PCR ) , we analyzed the flanking sequences of T-DNAs in transgenic rice plants , in which the resistance gene for rice bacterial blight disease , Xa21 , had been integrated stably .
[ Sen. 3, subscore: 1.00 ]: Sequence analysis of 24 fragments amplified by TAIL-PCR showed that of them 14 were rice genomic DNA , 9 contained vector backbone sequences , and one was a fragment of the exogenous gene Xa21 .
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Score: 2.00 |
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| | Journal: Biochem . Genet . Citation: V : 39 ( 7-8 ) P : 261-78 | | Literature: oryza Field: abstract Doc ID: pub11590832 Accession (PMID): 11590832 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The basis of selection of these DNA markers was their close linkage to xa5 , xa13 , and Xa21 genes and their positions on the rice genetic map relative to bacterial blight resistance genes .
[ Sen. 4, subscore: 1.00 ]: Eight lines were found to contain the xa5 gene while two lines contained Xa21 gene and none of the lines contained the xa13 gene with the exception of its near-isogenic line .
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Score: 2.00 |
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| | Journal: Citation: V : 33 ( 6 ) P : 710-2 | | Literature: oryza Field: abstract Doc ID: pub15727184 Accession (PMID): 15727184 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: OBJECTIVE : To observe the effects of genetically modified rice with Xa21 on the development of rat embryos .
[ Sen. 8, subscore: 1.00 ]: CONCLUSION : Compared with the non-transgenic rice , transgenic rice modified with Xa21 gene showed no significant differences in rat pregnant rate and embryo development .
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Score: 2.00 |
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| | Journal: Citation: V : 16 ( 1 ) P : 115-8 | | Literature: oryza Field: abstract Doc ID: pub15852969 Accession (PMID): 15852969 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The in vitro pollen germination rates of five transgenic rice lines transformed respectively with bar , crylAb , BADH and Xa21 gene were investigated , and compared with their relative receptor rice varieties .
[ Sen. 5, subscore: 1.00 ]: Investigation on the hybrid seed set of 26 hand-crosses showed that the inserted bar or crylAb gene had a significant effect on the hybrid seed set of receptors , while the effect of Xa21 gene was smaller .
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Score: 2.00 |
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| | Journal: Proc . Natl . Acad . Sci . USA Citation: V : 103 ( 49 ) P : 18395-400 | | Literature: oryza Field: abstract Doc ID: pub17082309 Accession (PMID): 17082309 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The rice pathogen recognition receptor , XA21 , confers resistance to Xanthomonas oryzae pv . oryzae strains producing the AvrXa21 elicitor .
[ Sen. 3, subscore: 1.00 ]: Xa21 codes for a receptor-like kinase consisting of an extracellular leucine-rich repeat domain , a transmembrane domain , and a cytoplasmic kinase domain .
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Score: 2.00 |
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| | Journal: Sci .
China , C , Life Sci .
Citation: V : 49 ( 5 ) P : 414-28 | | Literature: oryza Field: abstract Doc ID: pub17172048 Accession (PMID): 17172048 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Using this protocol , we obtained 145 independent green transformants from five cultivars of japonica rice by transformation with a binary vector pCXK1301 bearing the rice gene , Xa21 for resistance to bacterial blight , of which 140 were further confirmed by PCR and Southern hybridization analysis , including haploids ( 32 . 1% ) , diploids ( 62 . 1% ) and mixoploids ( 7 . 5% ) .
[ Sen. 5, subscore: 1.00 ]: Finally , by including 28 from colchicine induced or spontaneous diploidization of haploids later after transformation , a total of 43 doubled haploids ( 30 . 7% ) of Xa21 transgenic lines were obtained .
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