| 499 matches found in 345 documents. Search time: 0.033 seconds. |
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Score: 11.00 |
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| Title: Infrared photodissociation of a water molecule from a flexible molecule-H ( 2 ) O complex : Rates and conformational product yields following XH stretch excitation .
| | Author: Clarkson JR Herbert JM Zwier TS .
| | Journal: Citation: V : 126 ( 13 ) P : 134306 Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17430031 Accession (PMID): 17430031 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: Infrared-ultraviolet hole-burning and hole-filling spectroscopies have been used to study IR-induced dissociation of the tryptamineH ( 2 ) O and tryptamineD ( 2 ) O complexes .
[ Sen. 2, subscore: 2.00 ]: Upon complexation of a single water molecule , the seven conformational isomers of tryptamine collapse to a single structure that retains the same ethylamine side chain conformation present in the most highly populated conformer of tryptamine monomer .
[ Sen. 5, subscore: 2.00 ]: The observed threshold for dissociation occurred at 2872 cm ( -1 ) in tryptamineH ( 2 ) O and at 2869 cm ( -1 ) in tryptamineD ( 2 ) O , with no dissociation occurring on the time scale of the experiment ( approximately 2 mus ) at 2745 cm ( -1 ) .
[ Sen. 6, subscore: 2.00 ]: The dissociation time constants varied from approximately 200 ns for the 2869 cm ( -1 ) band of tryptamineD ( 2 ) O to approximately 25 ns for the 2872 cm ( -1 ) band of tryptamineH ( 2 ) O This large isotope dependence is associated with a zero-point energy effect that increases the binding energy of the deuterated complex by approximately 190 cm ( -1 ) , thereby reducing the excess energy available at the same excitation energy .
[ Sen. 3, subscore: 1.00 ]: Infrared excitation of the tryptamineH ( 2 ) O complex was carried out using a series of infrared absorptions spanning the range of 2470-3715 cm ( -1 ) .
[ Sen. 8, subscore: 1.00 ]: At all wavelengths , the observed products in the presence of collisions are dominated by conformers A and B of tryptamine monomer , with small contributions from the other minor conformers .
[ Sen. 9, subscore: 1.00 ]: In addition , right at threshold ( 2869 cm ( -1 ) ) , tryptamineD ( 2 ) O dissociates exclusively to conformer A in the absence of collisions with helium , while both A and B conformational products are observed in the presence of collisions with helium .
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Score: 10.00 |
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| Title: In vitro inhibition of the activation of Pro-matrix Metalloproteinase 1 ( Pro-MMP-1 ) and Pro-matrix metalloproteinase 9 ( Pro-MMP-9 ) by rice and soybean Bowman-Birk inhibitors .
| | Author: Bawadi HA Antunes TM Shih F Losso JN .
| | Journal: J Agric .
Food Chem .
Citation: V : 52 ( 15 ) P : 4730-6 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15264907 Accession (PMID): 15264907 | Matching Sentences:
[ Sen. 2, subscore: 3.00 ]: Heparin-enhanced zymography analysis of pro-MMP-1 , trypsin-activated MMP-1 , and a mixture of pro-MMP-1-trypsin-rBBI showed clear zones associated with trypsin-activated MMP-1 and the absence of clear zones in lanes containing pro-MMP-1 or a mixture of pro-MMP-1 , trypsin , and rBBI .
[ Sen. 1, subscore: 2.00 ]: The in vitro inhibitory activity of the rice Bowman-Birk inhibitor ( rBBI ) or soybean Bowman-Birk inhibitor ( sBBI ) against trypsin-catalyzed activation of pro-matrix metalloproteinase 1 or 9 ( pro-MMP-1 or pro-MMP-9 ) , respectively , was investigated using electrophoresis with silver staining , heparin-enhanced zymography , biotinylated gelatin , Biotrak assay , and fluorescence quenched substrate hydrolysis . rBBI at concentrations of 0 . 08-0 . 352 mg/mL dose-dependently inhibited the in vitro activation of 45 microg/mL pro-MMP-1 by trypsin .
[ Sen. 3, subscore: 2.00 ]: The results of the Biotrak assay also indicated that rBBI dose-dependently suppressed the activation of pro-MMP-1 by trypsin . sBBI dose-dependently inhibited the activation of 100 microg/mL of pro-MMP-9 by trypsin .
[ Sen. 4, subscore: 2.00 ]: Biotinylated gelatin assays demonstrated that pro-MMP-9 or pro-MMP-9 in the presence of trypsin and BBI did not hydrolyze gelatin , whereas p-aminophenylmercury acetate ( APMA ) -activated MMP-9 and trypsin-activated MMP-9 caused significant hydrolysis of gelatin .
[ Sen. 5, subscore: 1.00 ]: Quenched fluorescence substrate hydrolysis for total MMP activity showed that pro-MMP-1 or pro-MMP-9 did not hydrolyze the substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 ; active MMP-1 or MMP-9 hydrolyzed the substrate , but lower substrate hydrolysis was obtained when pro-MMP-1 or pro-MMP-9 was incubated with trypsin in the presence of increasing concentrations of rBBI .
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Score: 10.00 |
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| Title: Probing the role of tryptophan-derived secondary metabolism in defense responses against Bipolaris oryzae infection in rice leaves by a suicide substrate of tryptophan decarboxylase .
| | Author: Ishihara A Nakao T Mashimo Y Murai M Ichimaru N Tanaka C Nakajima H Wakasa K Miyagawa H | | Journal: Phytochemistry Citation: V : 72 P : 7-13 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21112065 Accession (PMID): 21112065 | Matching Sentences:
[ Sen. 3, subscore: 3.00 ]: Serotonin is biosynthesized from tryptophan via tryptamine , and tryptophan decarboxylase ( TDC ) catalyzes the first committed reaction .
[ Sen. 9, subscore: 3.00 ]: Administrating tryptamine to S-alphaFMT-treated leaves restored accumulation of tryptophan-derived secondary metabolites as well as deposition of brown material In addition , tryptamine administration reduced damage caused by fungal infection .
[ Sen. 1, subscore: 1.00 ]: Tryptophan-derived secondary metabolites , including serotonin and its hydroxycinnamic acid amides , markedly accumulate in rice leaves in response to pathogen attack .
[ Sen. 4, subscore: 1.00 ]: In this study , ( S ) -alpha- ( fluoromethyl ) tryptophan ( S-alphaFMT ) was utilized to investigate the effects of the inhibition of TDC on the defense responses of rice leaves .
[ Sen. 7, subscore: 1.00 ]: Treatment of rice seedlings with S-alphaFMT suppressed accumulation of serotonin , tryptamine , and hydroxycinnamic acid amides of serotonin in a dose-dependent manner in B oryzae-inoculated leaves .
[ Sen. 10, subscore: 1.00 ]: Accordingly , the accumulation of tryptophan-derived secondary metabolites was suggested to be part of the effective defense mechanism of rice .
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Score: 7.00 |
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| Title: Identification of target genes conferring ethanol stress tolerance to Saccharomyces cerevisiae based on DNA microarray data analysis .
| | Author: Hirasawa T Yoshikawa K Nakakura Y Nagahisa K Furusawa C Katakura Y Shimizu H Shioya S | | Journal: J Biotechnol Citation: V : 131 P : 34-44 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17604866 Accession (PMID): 17604866 | Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: Moreover , the addition of tryptophan to the culture medium and overexpression of tryptophan permease gene conferred ethanol stress tolerance to yeast cells .
[ Sen. 9, subscore: 2.00 ]: Tryptophan supplementation to culture and overexpression of the tryptophan permease gene are also effective for the increase in ethanol stress tolerance .
[ Sen. 5, subscore: 1.00 ]: The integration of the DNA microarray data and the ethanol sensitivity data of knockout strains suggests that the enhancement of expression of genes related to tryptophan biosynthesis might confer the ethanol stress tolerance to yeast cells .
[ Sen. 6, subscore: 1.00 ]: Indeed , the strains overexpressing tryptophan biosynthesis genes showed a stress tolerance to 5% ethanol .
[ Sen. 8, subscore: 1.00 ]: These results indicate that overexpression of the genes for trypophan biosynthesis increases the ethanol stress tolerance .
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Score: 7.00 |
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| Title: Serotonin accumulation in transgenic rice by over-expressing tryptophan decarboxylase results in a dark brown phenotype and stunted growth .
| | Author: Kanjanaphachoat P Wei BY Lo SF Wang IW Wang CS Yu SM Yen ML Chiu SH Lai CC Chen LJ | | Journal: Plant Mol Biol Citation: V : 78 P : 525-43 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22297847 Accession (PMID): 22297847 | Matching Sentences:
[ Sen. 3, subscore: 4.00 ]: Enzymatic assays of both recombinant proteins showed tryptophan decarboxylase activities that converted tryptophan to tryptamine , which could be converted to serotonin by a constitutively expressed tryptamine 5 hydroxylase ( T5H ) in rice plants .
[ Sen. 2, subscore: 1.00 ]: This mutant contained a copy of the T-DNA tag inserted at the location where the expression of two putative tryptophan decarboxylase genes , TDC-1 and TDC-3 , were activated .
[ Sen. 6, subscore: 1.00 ]: The levels of tryptamine and serotonin accumulation in these transgenic rice lines were also directly correlated with the expression levels of TDC-1 and TDC-3 .
[ Sen. 7, subscore: 1.00 ]: A mass spectrometry assay demonstrated that the darkbrown leaves and hulls in the TDC-overexpressing transgenic rice were caused by the accumulation of serotonin dimer and that the stunted growth and low fertility were also caused by the accumulation of serotonin and serotonin dimer , but not tryptamine .
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Score: 7.00 |
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| Title: Primary structure of 3-phosphoglycerate kinase from horse muscle .
II .
Amino acid sequence of cyanogen bromide peptides CB1-CB4 and CB6-CB14 , sequence of methionine-containing regions , and complete sequence of the enzyme .
| | Author: Merrett M | | Journal: J Biol . Chem . Citation: V : 256 ( 20 ) P : 10293-305 Year: 1981 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub7287713 Accession (PMID): 7287713 | Matching Sentences:
[ Sen. 9, subscore: 3.00 ]: Tryptophan-containing tryptic peptides were also isolated , and their sequence analysis enabled the positions of the four tryptophanyl residues to be established .
[ Sen. 6, subscore: 1.00 ]: This peptide appeared to have arisen as the result of a cyanogen bromide cleavage on the carboxyl side of a tryptophanyl residue .
[ Sen. 7, subscore: 1.00 ]: The sequences of these 13 cyanogen bromide peptides , together with that of CB1 , reported previously ( Hardy , G W , Darbre , A , and Merrett , M ( 1981 ) J Biol . Chem . 256 , 10284-10292 ) , have been combined with data obtained from the sequence analysis of tryptic peptides derived from the methionine-containing regions of the enzyme to determine the complete sequence .
[ Sen. 8, subscore: 1.00 ]: Isolation of these overlap peptides was facilitated by the labeling of methionyl residues with iodo [ 2-14C ] acetic acid prior to tryptic digestion .
[ Sen. 10, subscore: 1.00 ]: In this way , the problem caused by tryptophan degradation during the preparation of the cyanogen bromide fragments was overcome .
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Score: 7.00 |
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| Title: The tryptophan content in protein of cereal grains and legume seeds as a function of nitrogen content .
A reappraisal of tryptophan score .
| | Author: Delhaye S Landry J | | Journal: Adv . Exp . Med . Biol . Citation: V : 398 ( ) P : 661-4 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8906341 Accession (PMID): 8906341 | Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: In conclusion : ( 1 ) linear relationships between tryptophan and nitrogen have a low predictive value ; ( 2 ) the nutritional score of tryptophan of foods and feeds , as calculated from the determination of tryptophan using a procedure involving alkaline hydrolysis , is generally underestimated by 10% .
[ Sen. 3, subscore: 2.00 ]: The data show that previous reports of similar relationships underestimate tryptophan by 10 +/- 5% owing to tryptophan degradation during alkaline hydrolysis preparatory to analysis ; ( 2 ) the linear correlations between [ Trp ] N and 1/ [ N ] DM , resulting from linear relationships [ Trp ] DM and [ N ] DM display coefficients of determination ( r2 ) far lower than 1 and similar to those found for linear correlations between [ Trp ] N and [ N ] DM ; ( 3 ) [ Trp ] N increases with [ N ] DM increasing for rice and pearl millet while it decreases for all other species .
[ Sen. 1, subscore: 1.00 ]: The tryptophan content ( [ Trp ] ) in the dry matter ( DM ) of wheat , maize , barley , sorghum , rice , pearl millet grains , pea and broad bean seeds is evaluated from samples , with various nitrogen contents ( [ N ] DM ) and genotypes , using a procedure that has been shown to be strictly quantitative .
[ Sen. 2, subscore: 1.00 ]: The determination of linear correlations between [ Trp ] DM and [ N ] DM , and between the tryptophan content in protein ( [ Trp ] N ) and [ N ] DM or 1/ [ N ] DM for every species leads to the following observations : ( 1 ) [ Trp ] DM and [ N ] DM are linearly related .
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Score: 6.00 |
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| Title: Characterization of tryptamine 5-hydroxylase and serotonin synthesis in rice plants .
| | Author: Kang S Kang K Lee K Back K | | Journal: Plant Cell Rep Citation: V : 26 P : 2009-15 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17639402 Accession (PMID): 17639402 | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Tryptamine 5-hydroxylase ( T5H ) , which converts tryptamine into serotonin , was found as a soluble enzyme that had maximal activity in the roots .
[ Sen. 9, subscore: 2.00 ]: However , no significant increase in serotonin was observed in rice it issues grown in the presence of tryptophan , suggesting that tryptamine is a bottleneck intermediate substrate for serotonin synthesis .
[ Sen. 7, subscore: 1.00 ]: Tetrahydropterine-dependent T5H activity was inhibited by tyramine , tryptophan , 5-OH-tryptophan , and octopamine , but remained unaltered by dopamine in vitro .
[ Sen. 8, subscore: 1.00 ]: The it issues of rice seedlings grown in the presence of tryptamine exhibited a dose-dependent increase in serotonin in parallel with enhanced T5H enzyme activity .
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Score: 6.00 |
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| Title: Overproduction of indole acetic acid in Azospirillum lipoferum using the Escherichia coli trp operon .
| | Author: Cho MJ Gal SW Choi YJ Yoon HW Kim CY Hong JC Bahk JD .
| | Journal: Microb .
Releases Citation: V : 1 ( 4 ) P : 197-202 Year: 1993 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8281358 Accession (PMID): 8281358 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: A recombinant plasmid carrying the trp operon from Escherichia coli , which synthesizes tryptophan from chorismate , was constructed by using a broad host range plasmid vector pRK290 ; a mutant trp plasmid for tryptophan overproduction was then selected .
[ Sen. 5, subscore: 2.00 ]: As expected , the amount of tryptophan biosynthesis in A lipoferum KY6 ( pMJC101 ) was increased approximately 100-fold as compared with the wild type , which led to overproduction of indole acetic acid even without addition of exogenous tryptophan .
[ Sen. 3, subscore: 1.00 ]: Anthranilate synthetase is known to be the trpE gene product which plays a key role in the regulatory step in the feedback control of tryptophan biosynthesis .
[ Sen. 4, subscore: 1.00 ]: The enzyme activity of the Azospirillum lipoferum KY6 carrying pMJC1 or pMJC101 was respectively 7 and 30-fold higher than that of the wild type in the presence of 10 ( -4 ) M tryptophan .
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Score: 5.00 |
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| Title: [ Research of indole-3-acetic acid biosynthetic pathway of Klebsiella oxytoca SG-11 by HPLC and GC-MS ] | | Author: LEZX Song W | | Journal: Citation: V : 18 ( 4 ) P : 328-31 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12541509 Accession (PMID): 12541509 | Matching Sentences:
[ Sen. 10, subscore: 2.00 ]: The intermediate determination of tryptamine , indole-3-acetamide , tryptophol and indole-3-acetonitrile indirectly indicated that IAA was biosynthesized in a pathway of indole-3-pyruvic acid .
[ Sen. 13, subscore: 2.00 ]: As reversible conversion existed between indole-3 pyruvic aldehyde and tryptophol , the presence of tryptophol also proved the pathway of indole-3-pyruvic acid in the synthesis of IAA by Klebsiella axytoca SG-11 .
[ Sen. 11, subscore: 1.00 ]: Meanwhile , tryptophol in metabolite of SG-11 was verified by GC/MS .
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Score: 5.00 |
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| Title: Increased tryptophan decarboxylase and monoamine oxidase activities induce Sekiguchi lesion formation in rice infected with Magnaporthe grisea .
| | Author: Ueno M Shibata H Kihara J Honda Y Arase S | | Journal: Plant J Citation: V : 36 ( 2 ) P : 215-28 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14535886 Accession (PMID): 14535886 | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Sekiguchi lesion was also induced by treatments with tryptamine and beta-phenylethylamine , which are substrates for MAO , but was not induced by non-substrates such as indole-3-propionic acid , ( +/- ) -phenylethylamine and tryptophan under light .
[ Sen. 1, subscore: 1.00 ]: Sekiguchi lesion ( sl ) -mutant rice infected with Magnaporthe grisea showed increased light-dependent tryptophan decarboxylase ( TDC ) and monoamine oxidase ( MAO ) activities .
[ Sen. 4, subscore: 1.00 ]: Spore germination fluid ( SGF ) of M grisea also induced Sekiguchi lesion formation accompanied by increased enzymes activities and tryptamine accumulation .
[ Sen. 6, subscore: 1.00 ]: Light-dependent induction of Sekiguchi lesion by tryptamine was significantly inhibited in the presence of MAO inhibitors , metalaxyl and semicarbazide , and H2O2-scavengers , ascorbic acid and catalase .
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Score: 5.00 |
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| Title: Metabolic profiling of tryptophan-overproducing rice calli that express a feedback-insensitive alpha subunit of anthranilate synthase .
| | Author: Morino K Matsuda F Miyazawa H Sukegawa A Miyagawa H Wakasa K | | Journal: Plant Cell Physiol .
Citation: V : 46 ( 3 ) P : 514-21 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15695448 Accession (PMID): 15695448 | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Only a limited effect on the pattern of major aromatic compounds was observed in tryptophan-accumulating transgenic rice lines , with the exception of an approximately 80-fold increase in the amount of tryptophan .
[ Sen. 2, subscore: 1.00 ]: Nipponbare ) and tryptophan-overproducing transgenic rice lines that express a gene ( OASA1D ) for a feedback-insensitive alpha subunit of anthranilate synthase .
[ Sen. 7, subscore: 1.00 ]: The levels of free and conjugated forms of indole-3-acetic acid ( IAA ) , a plant hormone derived from the tryptophan biosynthetic pathway , were determined separately by liquid chromatography and tandem mass spectrometry ( LC-MS/MS ) .
[ Sen. 8, subscore: 1.00 ]: The amounts of both free IAA and its conjugates were increased in the transgenic calli , suggesting that the activity of anthranilate synthase or the concentration of tryptophan ( or both ) is an important determinant of IAA biosynthesis .
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Score: 5.00 |
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| Title: Characterization of rice tryptophan decarboxylases and their direct involvement in serotonin biosynthesis in transgenic rice .
| | Author: Kang S Kang K Lee K Back K | | Journal: Planta Citation: V : 227 P : 263-72 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17763868 Accession (PMID): 17763868 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: L-Tryptophan decarboxylase ( TDC ) and L-tyrosine decarboxylase ( TYDC ) belong to a family of aromatic L-amino acid decarboxylases and catalyze the conversion of tryptophan and tyrosine into tryptamine and tyramine , respectively .
[ Sen. 6, subscore: 2.00 ]: The recombinant TDC protein with the greatest TDC activity showed a K ( m ) of 0 . 69 mM for tryptophan , and its activity was not inhibited by phenylalanine or tyrosine , indicating a high level of substrate specificity toward tryptophan .
[ Sen. 7, subscore: 1.00 ]: The ectopic expression of the three cDNA clones in rice led to the abundant production of the products of the encoded enzymes , tyramine and tryptamine .
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Score: 5.00 |
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| Title: Senescence Induced Serotonin Biosynthesis and Its Role in Delaying Senescence in Rice Leaves .
| | Author: Kang K Kim YS Park S Back K | | Journal: Plant Physiol Citation: V : P : Year: 2009 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub19439571 Accession (PMID): 19439571 | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: In this study , we found that serotonin is greatly accumulated in rice leaves undergoing senescence induced by either nutrient deprivation or detachment , and its synthesis is closely coupled with transcriptional and enzymatic induction of the tryptophan biosynthetic genes as well as tryptophan decarboxylase ( TDC ) .
[ Sen. 7, subscore: 2.00 ]: Results of TDC overexpression and TDC RNAi plants suggest that TDC plays a rate-limiting role for serotonin accumulation , but the synthesis of serotonin depends on an absolute amount of tryptophan accumulation by the coordinate induction of the tryptophan biosynthetic genes .
[ Sen. 6, subscore: 1.00 ]: The senescence-retarding activity of serotonin is associated with its high antioxidant activity compared to either tryptophan or chlorogenic acid .
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Score: 5.00 |
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| Title: silencing coi1 in rice increases susceptibility to chewing insects and impairs inducible defense .
| | Author: Ye M Luo SM Xie JF Li YF Xu T Liu Y Song YY Zhu-Salzman K Zeng RS | | Journal: PLoS One Citation: V : 7 P : e36214 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22558386 Accession (PMID): 22558386 | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: The abundance of trypsin protease inhibitor ( TrypPI ) , and the enzymatic activities of polyphenol oxidase ( PPO ) and peroxidase ( POD ) were enhanced in response to both LF and BPH infestation , but the activity of lipoxygenase ( LOX ) was only induced by LF .
[ Sen. 8, subscore: 1.00 ]: Silencing OsCOI1 did not alter LF-induced LOX activity and JA content , but it led to a reduction in the TrypPI content , POD and PPO activity by 62 . 3% , 48 . 5% and 27 . 2% , respectively .
[ Sen. 9, subscore: 1.00 ]: In addition , MeJA-induced TrypPI and POD activity were reduced by 57 . 2% and 48 . 2% in OsCOI1 RNAi plants .
[ Sen. 10, subscore: 1.00 ]: These results suggest that OsCOI1 is an indispensable signaling component , controlling JA-regulated defense against chewing insect ( LF ) in rice plants , and COI1 is also required for induction of TrypPI , POD and PPO in rice defense response to LF infestation .
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Score: 4.00 |
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| Title: Some tryptophan pathways in the phytopathogen Xanthomonas oryzae pv . oryzae .
| | Author: Ansari MM Sridhar R | | Journal: Folia Microbiol .
( Praha ) Citation: V : 45 ( 6 ) P : 531-7 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11501419 Accession (PMID): 11501419 | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: The isolates metabolized tryptophan through two different routes and the isolates vary in the pathway of tryptophan utilization .
[ Sen. 5, subscore: 1.00 ]: The IAA-positive isolates converted tryptophan to IAA as the end product , whereas the IAA-negative isolates formed anthranilate as an intermediate metabolite and finally produced pyrocatechol via the kynurenine pathway .
[ Sen. 6, subscore: 1.00 ]: Quantification of tryptophan metabolism revealed that the maximum production of IAA and pyrocatechol in culture occurred during 2-d incubation at 30 +/- 2 degrees C
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Score: 4.00 |
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| Title: Characterization of the altered anthranilate synthase in 5-methyltryptophan-resistant rice mutants .
| | Author: Kim DS Lee IS Jang CS Kang SY Seo YW .
| | Journal: Plant Cell Rep .
Citation: V : 24 ( 6 ) P : 357-65 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15776237 Accession (PMID): 15776237 | Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: The transcript levels of putative OASA2 , which is one of the key-regulating enzyme subunits in the tryptophan biosynthesis pathway , were studied in the control and 5MT-resistant mutant lines subjected to inhibition by two tryptophan analogs ( 5MT and alphaMT ) and to other abiotic stresses ( ABA , NaCl , and cold ) .
[ Sen. 4, subscore: 1.00 ]: In a kinetic analysis with tryptophan , an anthranilate synthase of the mutant lines was insensitive to feedback inhibition .
[ Sen. 8, subscore: 1.00 ]: Significant increases among the amino acids of the MR lines were observed for tryptophan , phenylalanine , and tyrosine , which had been biosynthesized through the shikimate pathway .
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Score: 4.00 |
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| Title: Tryptophan Enhancement of Somatic Embryogenesis in Rice .
| | Author: Siriwardana S Nabors MW .
| | Journal: Citation: V : 73 ( 1 ) P : 142-146 Year: 1983 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16663163 Accession (PMID): 16663163 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: High concentrations of tryptophan increased the formation of embryogenic callus in three rice cultivars ( Oryza sativa L Calrose 76 , Pokkali , and IR 36 ) but not in four others ( Mahsuri , Bg 400-1 , H ( 4 ) , and Giza 159 ) .
[ Sen. 5, subscore: 1.00 ]: The best concentration of tryptophan for Pokkali and Calrose 76 was 100 micrograms per milliliter , and for IR 36 , 50 micrograms per milliliter .
[ Sen. 6, subscore: 1.00 ]: Indoleacetic acid at 100 micrograms per milliliter promoted an effect similar to that of tryptophan on Calrose 76 .
[ Sen. 7, subscore: 1.00 ]: The difference between japonica ( Calrose 76 , Giza 159 ) and indica ( Pokkali , IR 36 ) varieties is not the causal factor for the difference in response to tryptophan .
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Score: 4.00 |
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| Title: Purification and Characterization of a Trypsin Inhibitor from Plathymenia foliolosa Seeds .
| | Author: da Silveira Ramos V de Souza Silva G das Gracas Machado Freire M Lima Tavares Machado O Postali Parra JR Rodrigues Macedo ML | | Journal: J Agric Food Chem Citation: V : P : Year: 2008 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub18991455 Accession (PMID): 18991455 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: A novel trypsin inhibitor ( PFTI ) was isolated from Plathymenia foliolosa ( Benth . ) seeds by gel filtration chromatography on a Sephadex G-100 , DEAE-Sepharose , and trypsin-Sepharose columns .
[ Sen. 3, subscore: 1.00 ]: PFTI inhibited bovine trypsin and bovine chymotrypsin with equilibrium dissociation constants ( K i ) of 4 x 10 ( -8 ) and 1 . 4 x 10 ( -6 ) M , respectively .
[ Sen. 10, subscore: 1.00 ]: PFTI showed significant inhibitory activity against trypsin-like proteases present in the larval midguts on A kuehniella and D saccharalis and could suppress the growth of larvae .
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Score: 4.00 |
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| Title: cDNA cloning , heterogeneous expression and biochemical characterization of a novel trypsin-like protease from Nilaparvata lugens .
| | Author: Yang Z Xia X Wang X He G | | Journal: Z Naturforsch C Citation: V : 65 P : 109-18 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20355330 Accession (PMID): 20355330 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A reverse transcription-polymerase chain reaction ( RT-PCR ) strategy was used to clone diverse trypsin-like protease gene transcripts from midguts of the brown planthopper Nilaparvata lugens Stal ( Homoptera : Delphacidae ) .
[ Sen. 2, subscore: 1.00 ]: Six individual trypsin-like protease transcripts were identified .
[ Sen. 4, subscore: 1.00 ]: The cDNA contained an 1128-bp open reading frame encoding a putative protein of 375 amino acids with typical features of the trypsin-like protease .
[ Sen. 7, subscore: 1.00 ]: The trypsin-like protease is therefore likely one of the major digestive proteases responsible for protein hydrolysis in N lugens gut , and multiple gene families encoding digestive proteases may help in adaptation of this sap-sucker to different rice varieties .
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