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Score: 11.00
Author: Clarkson JR Herbert JM Zwier TS .
Journal: Citation: V : 126 ( 13 ) P : 134306 Year: 2007 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub17430031 Accession (PMID): 17430031
Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: Infrared-ultraviolet hole-burning and hole-filling spectroscopies have been used to study IR-induced dissociation of the tryptamineH ( 2 ) O and tryptamineD ( 2 ) O complexes .
[ Sen. 2, subscore: 2.00 ]: Upon complexation of a single water molecule , the seven conformational isomers of tryptamine collapse to a single structure that retains the same ethylamine side chain conformation present in the most highly populated conformer of tryptamine monomer .
[ Sen. 5, subscore: 2.00 ]: The observed threshold for dissociation occurred at 2872 cm ( -1 ) in tryptamineH ( 2 ) O and at 2869 cm ( -1 ) in tryptamineD ( 2 ) O , with no dissociation occurring on the time scale of the experiment ( approximately 2 mus ) at 2745 cm ( -1 ) .
[ Sen. 6, subscore: 2.00 ]: The dissociation time constants varied from approximately 200 ns for the 2869 cm ( -1 ) band of tryptamineD ( 2 ) O to approximately 25 ns for the 2872 cm ( -1 ) band of tryptamineH ( 2 ) O This large isotope dependence is associated with a zero-point energy effect that increases the binding energy of the deuterated complex by approximately 190 cm ( -1 ) , thereby reducing the excess energy available at the same excitation energy .
[ Sen. 3, subscore: 1.00 ]: Infrared excitation of the tryptamineH ( 2 ) O complex was carried out using a series of infrared absorptions spanning the range of 2470-3715 cm ( -1 ) .
[ Sen. 8, subscore: 1.00 ]: At all wavelengths , the observed products in the presence of collisions are dominated by conformers A and B of tryptamine monomer , with small contributions from the other minor conformers .
[ Sen. 9, subscore: 1.00 ]: In addition , right at threshold ( 2869 cm ( -1 ) ) , tryptamineD ( 2 ) O dissociates exclusively to conformer A in the absence of collisions with helium , while both A and B conformational products are observed in the presence of collisions with helium .
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Score: 10.00
Author: Bawadi HA Antunes TM Shih F Losso JN .
Journal: J Agric . Food Chem . Citation: V : 52 ( 15 ) P : 4730-6 Year: 2004 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub15264907 Accession (PMID): 15264907
Matching Sentences:
[ Sen. 2, subscore: 3.00 ]: Heparin-enhanced zymography analysis of pro-MMP-1 , trypsin-activated MMP-1 , and a mixture of pro-MMP-1-trypsin-rBBI showed clear zones associated with trypsin-activated MMP-1 and the absence of clear zones in lanes containing pro-MMP-1 or a mixture of pro-MMP-1 , trypsin , and rBBI .
[ Sen. 1, subscore: 2.00 ]: The in vitro inhibitory activity of the rice Bowman-Birk inhibitor ( rBBI ) or soybean Bowman-Birk inhibitor ( sBBI ) against trypsin-catalyzed activation of pro-matrix metalloproteinase 1 or 9 ( pro-MMP-1 or pro-MMP-9 ) , respectively , was investigated using electrophoresis with silver staining , heparin-enhanced zymography , biotinylated gelatin , Biotrak assay , and fluorescence quenched substrate hydrolysis . rBBI at concentrations of 0 . 08-0 . 352 mg/mL dose-dependently inhibited the in vitro activation of 45 microg/mL pro-MMP-1 by trypsin .
[ Sen. 3, subscore: 2.00 ]: The results of the Biotrak assay also indicated that rBBI dose-dependently suppressed the activation of pro-MMP-1 by trypsin . sBBI dose-dependently inhibited the activation of 100 microg/mL of pro-MMP-9 by trypsin .
[ Sen. 4, subscore: 2.00 ]: Biotinylated gelatin assays demonstrated that pro-MMP-9 or pro-MMP-9 in the presence of trypsin and BBI did not hydrolyze gelatin , whereas p-aminophenylmercury acetate ( APMA ) -activated MMP-9 and trypsin-activated MMP-9 caused significant hydrolysis of gelatin .
[ Sen. 5, subscore: 1.00 ]: Quenched fluorescence substrate hydrolysis for total MMP activity showed that pro-MMP-1 or pro-MMP-9 did not hydrolyze the substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 ; active MMP-1 or MMP-9 hydrolyzed the substrate , but lower substrate hydrolysis was obtained when pro-MMP-1 or pro-MMP-9 was incubated with trypsin in the presence of increasing concentrations of rBBI .
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Score: 10.00
Author: Ishihara A Nakao T Mashimo Y Murai M Ichimaru N Tanaka C Nakajima H Wakasa K Miyagawa H
Journal: Phytochemistry Citation: V : 72 P : 7-13 Year: 2011 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub21112065 Accession (PMID): 21112065
Matching Sentences:
[ Sen. 3, subscore: 3.00 ]: Serotonin is biosynthesized from tryptophan via tryptamine , and tryptophan decarboxylase ( TDC ) catalyzes the first committed reaction .
[ Sen. 9, subscore: 3.00 ]: Administrating tryptamine to S-alphaFMT-treated leaves restored accumulation of tryptophan-derived secondary metabolites as well as deposition of brown material In addition , tryptamine administration reduced damage caused by fungal infection .
[ Sen. 1, subscore: 1.00 ]: Tryptophan-derived secondary metabolites , including serotonin and its hydroxycinnamic acid amides , markedly accumulate in rice leaves in response to pathogen attack .
[ Sen. 4, subscore: 1.00 ]: In this study , ( S ) -alpha- ( fluoromethyl ) tryptophan ( S-alphaFMT ) was utilized to investigate the effects of the inhibition of TDC on the defense responses of rice leaves .
[ Sen. 7, subscore: 1.00 ]: Treatment of rice seedlings with S-alphaFMT suppressed accumulation of serotonin , tryptamine , and hydroxycinnamic acid amides of serotonin in a dose-dependent manner in B oryzae-inoculated leaves .
[ Sen. 10, subscore: 1.00 ]: Accordingly , the accumulation of tryptophan-derived secondary metabolites was suggested to be part of the effective defense mechanism of rice .
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Score: 7.00
Author: Hirasawa T Yoshikawa K Nakakura Y Nagahisa K Furusawa C Katakura Y Shimizu H Shioya S
Journal: J Biotechnol Citation: V : 131 P : 34-44 Year: 2007 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub17604866 Accession (PMID): 17604866
Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: Moreover , the addition of tryptophan to the culture medium and overexpression of tryptophan permease gene conferred ethanol stress tolerance to yeast cells .
[ Sen. 9, subscore: 2.00 ]: Tryptophan supplementation to culture and overexpression of the tryptophan permease gene are also effective for the increase in ethanol stress tolerance .
[ Sen. 5, subscore: 1.00 ]: The integration of the DNA microarray data and the ethanol sensitivity data of knockout strains suggests that the enhancement of expression of genes related to tryptophan biosynthesis might confer the ethanol stress tolerance to yeast cells .
[ Sen. 6, subscore: 1.00 ]: Indeed , the strains overexpressing tryptophan biosynthesis genes showed a stress tolerance to 5% ethanol .
[ Sen. 8, subscore: 1.00 ]: These results indicate that overexpression of the genes for trypophan biosynthesis increases the ethanol stress tolerance .
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Score: 7.00
Author: Kanjanaphachoat P Wei BY Lo SF Wang IW Wang CS Yu SM Yen ML Chiu SH Lai CC Chen LJ
Journal: Plant Mol Biol Citation: V : 78 P : 525-43 Year: 2012 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub22297847 Accession (PMID): 22297847
Matching Sentences:
[ Sen. 3, subscore: 4.00 ]: Enzymatic assays of both recombinant proteins showed tryptophan decarboxylase activities that converted tryptophan to tryptamine , which could be converted to serotonin by a constitutively expressed tryptamine 5 hydroxylase ( T5H ) in rice plants .
[ Sen. 2, subscore: 1.00 ]: This mutant contained a copy of the T-DNA tag inserted at the location where the expression of two putative tryptophan decarboxylase genes , TDC-1 and TDC-3 , were activated .
[ Sen. 6, subscore: 1.00 ]: The levels of tryptamine and serotonin accumulation in these transgenic rice lines were also directly correlated with the expression levels of TDC-1 and TDC-3 .
[ Sen. 7, subscore: 1.00 ]: A mass spectrometry assay demonstrated that the darkbrown leaves and hulls in the TDC-overexpressing transgenic rice were caused by the accumulation of serotonin dimer and that the stunted growth and low fertility were also caused by the accumulation of serotonin and serotonin dimer , but not tryptamine .
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Score: 7.00
Author: Merrett M
Journal: J Biol . Chem . Citation: V : 256 ( 20 ) P : 10293-305 Year: 1981 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub7287713 Accession (PMID): 7287713
Matching Sentences:
[ Sen. 9, subscore: 3.00 ]: Tryptophan-containing tryptic peptides were also isolated , and their sequence analysis enabled the positions of the four tryptophanyl residues to be established .
[ Sen. 6, subscore: 1.00 ]: This peptide appeared to have arisen as the result of a cyanogen bromide cleavage on the carboxyl side of a tryptophanyl residue .
[ Sen. 7, subscore: 1.00 ]: The sequences of these 13 cyanogen bromide peptides , together with that of CB1 , reported previously ( Hardy , G W , Darbre , A , and Merrett , M ( 1981 ) J Biol . Chem . 256 , 10284-10292 ) , have been combined with data obtained from the sequence analysis of tryptic peptides derived from the methionine-containing regions of the enzyme to determine the complete sequence .
[ Sen. 8, subscore: 1.00 ]: Isolation of these overlap peptides was facilitated by the labeling of methionyl residues with iodo [ 2-14C ] acetic acid prior to tryptic digestion .
[ Sen. 10, subscore: 1.00 ]: In this way , the problem caused by tryptophan degradation during the preparation of the cyanogen bromide fragments was overcome .
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Score: 7.00
Author: Delhaye S Landry J
Journal: Adv . Exp . Med . Biol . Citation: V : 398 ( ) P : 661-4 Year: Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub8906341 Accession (PMID): 8906341
Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: In conclusion : ( 1 ) linear relationships between tryptophan and nitrogen have a low predictive value ; ( 2 ) the nutritional score of tryptophan of foods and feeds , as calculated from the determination of tryptophan using a procedure involving alkaline hydrolysis , is generally underestimated by 10% .
[ Sen. 3, subscore: 2.00 ]: The data show that previous reports of similar relationships underestimate tryptophan by 10 +/- 5% owing to tryptophan degradation during alkaline hydrolysis preparatory to analysis ; ( 2 ) the linear correlations between [ Trp ] N and 1/ [ N ] DM , resulting from linear relationships [ Trp ] DM and [ N ] DM display coefficients of determination ( r2 ) far lower than 1 and similar to those found for linear correlations between [ Trp ] N and [ N ] DM ; ( 3 ) [ Trp ] N increases with [ N ] DM increasing for rice and pearl millet while it decreases for all other species .
[ Sen. 1, subscore: 1.00 ]: The tryptophan content ( [ Trp ] ) in the dry matter ( DM ) of wheat , maize , barley , sorghum , rice , pearl millet grains , pea and broad bean seeds is evaluated from samples , with various nitrogen contents ( [ N ] DM ) and genotypes , using a procedure that has been shown to be strictly quantitative .
[ Sen. 2, subscore: 1.00 ]: The determination of linear correlations between [ Trp ] DM and [ N ] DM , and between the tryptophan content in protein ( [ Trp ] N ) and [ N ] DM or 1/ [ N ] DM for every species leads to the following observations : ( 1 ) [ Trp ] DM and [ N ] DM are linearly related .
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Score: 6.00
Author: Kang S Kang K Lee K Back K
Journal: Plant Cell Rep Citation: V : 26 P : 2009-15 Year: 2007 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub17639402 Accession (PMID): 17639402
Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Tryptamine 5-hydroxylase ( T5H ) , which converts tryptamine into serotonin , was found as a soluble enzyme that had maximal activity in the roots .
[ Sen. 9, subscore: 2.00 ]: However , no significant increase in serotonin was observed in rice it issues grown in the presence of tryptophan , suggesting that tryptamine is a bottleneck intermediate substrate for serotonin synthesis .
[ Sen. 7, subscore: 1.00 ]: Tetrahydropterine-dependent T5H activity was inhibited by tyramine , tryptophan , 5-OH-tryptophan , and octopamine , but remained unaltered by dopamine in vitro .
[ Sen. 8, subscore: 1.00 ]: The it issues of rice seedlings grown in the presence of tryptamine exhibited a dose-dependent increase in serotonin in parallel with enhanced T5H enzyme activity .
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Score: 6.00
Author: Cho MJ Gal SW Choi YJ Yoon HW Kim CY Hong JC Bahk JD .
Journal: Microb . Releases Citation: V : 1 ( 4 ) P : 197-202 Year: 1993 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub8281358 Accession (PMID): 8281358
Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: A recombinant plasmid carrying the trp operon from Escherichia coli , which synthesizes tryptophan from chorismate , was constructed by using a broad host range plasmid vector pRK290 ; a mutant trp plasmid for tryptophan overproduction was then selected .
[ Sen. 5, subscore: 2.00 ]: As expected , the amount of tryptophan biosynthesis in A lipoferum KY6 ( pMJC101 ) was increased approximately 100-fold as compared with the wild type , which led to overproduction of indole acetic acid even without addition of exogenous tryptophan .
[ Sen. 3, subscore: 1.00 ]: Anthranilate synthetase is known to be the trpE gene product which plays a key role in the regulatory step in the feedback control of tryptophan biosynthesis .
[ Sen. 4, subscore: 1.00 ]: The enzyme activity of the Azospirillum lipoferum KY6 carrying pMJC1 or pMJC101 was respectively 7 and 30-fold higher than that of the wild type in the presence of 10 ( -4 ) M tryptophan .
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Score: 5.00
Author: LEZX Song W
Journal: Citation: V : 18 ( 4 ) P : 328-31 Year: 2000 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub12541509 Accession (PMID): 12541509
Matching Sentences:
[ Sen. 10, subscore: 2.00 ]: The intermediate determination of tryptamine , indole-3-acetamide , tryptophol and indole-3-acetonitrile indirectly indicated that IAA was biosynthesized in a pathway of indole-3-pyruvic acid .
[ Sen. 13, subscore: 2.00 ]: As reversible conversion existed between indole-3 pyruvic aldehyde and tryptophol , the presence of tryptophol also proved the pathway of indole-3-pyruvic acid in the synthesis of IAA by Klebsiella axytoca SG-11 .
[ Sen. 11, subscore: 1.00 ]: Meanwhile , tryptophol in metabolite of SG-11 was verified by GC/MS .
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Score: 5.00
Author: Ueno M Shibata H Kihara J Honda Y Arase S
Journal: Plant J Citation: V : 36 ( 2 ) P : 215-28 Year: 2003 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub14535886 Accession (PMID): 14535886
Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Sekiguchi lesion was also induced by treatments with tryptamine and beta-phenylethylamine , which are substrates for MAO , but was not induced by non-substrates such as indole-3-propionic acid , ( +/- ) -phenylethylamine and tryptophan under light .
[ Sen. 1, subscore: 1.00 ]: Sekiguchi lesion ( sl ) -mutant rice infected with Magnaporthe grisea showed increased light-dependent tryptophan decarboxylase ( TDC ) and monoamine oxidase ( MAO ) activities .
[ Sen. 4, subscore: 1.00 ]: Spore germination fluid ( SGF ) of M grisea also induced Sekiguchi lesion formation accompanied by increased enzymes activities and tryptamine accumulation .
[ Sen. 6, subscore: 1.00 ]: Light-dependent induction of Sekiguchi lesion by tryptamine was significantly inhibited in the presence of MAO inhibitors , metalaxyl and semicarbazide , and H2O2-scavengers , ascorbic acid and catalase .
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Score: 5.00
Author: Morino K Matsuda F Miyazawa H Sukegawa A Miyagawa H Wakasa K
Journal: Plant Cell Physiol . Citation: V : 46 ( 3 ) P : 514-21 Year: 2005 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub15695448 Accession (PMID): 15695448
Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Only a limited effect on the pattern of major aromatic compounds was observed in tryptophan-accumulating transgenic rice lines , with the exception of an approximately 80-fold increase in the amount of tryptophan .
[ Sen. 2, subscore: 1.00 ]: Nipponbare ) and tryptophan-overproducing transgenic rice lines that express a gene ( OASA1D ) for a feedback-insensitive alpha subunit of anthranilate synthase .
[ Sen. 7, subscore: 1.00 ]: The levels of free and conjugated forms of indole-3-acetic acid ( IAA ) , a plant hormone derived from the tryptophan biosynthetic pathway , were determined separately by liquid chromatography and tandem mass spectrometry ( LC-MS/MS ) .
[ Sen. 8, subscore: 1.00 ]: The amounts of both free IAA and its conjugates were increased in the transgenic calli , suggesting that the activity of anthranilate synthase or the concentration of tryptophan ( or both ) is an important determinant of IAA biosynthesis .
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Score: 5.00
Author: Kang S Kang K Lee K Back K
Journal: Planta Citation: V : 227 P : 263-72 Year: 2007 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub17763868 Accession (PMID): 17763868
Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: L-Tryptophan decarboxylase ( TDC ) and L-tyrosine decarboxylase ( TYDC ) belong to a family of aromatic L-amino acid decarboxylases and catalyze the conversion of tryptophan and tyrosine into tryptamine and tyramine , respectively .
[ Sen. 6, subscore: 2.00 ]: The recombinant TDC protein with the greatest TDC activity showed a K ( m ) of 0 . 69 mM for tryptophan , and its activity was not inhibited by phenylalanine or tyrosine , indicating a high level of substrate specificity toward tryptophan .
[ Sen. 7, subscore: 1.00 ]: The ectopic expression of the three cDNA clones in rice led to the abundant production of the products of the encoded enzymes , tyramine and tryptamine .
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Score: 5.00
Author: Kang K Kim YS Park S Back K
Journal: Plant Physiol Citation: V : P : Year: 2009 Type: Publisher
Literature: oryza Field: abstract Doc ID: pub19439571 Accession (PMID): 19439571
Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: In this study , we found that serotonin is greatly accumulated in rice leaves undergoing senescence induced by either nutrient deprivation or detachment , and its synthesis is closely coupled with transcriptional and enzymatic induction of the tryptophan biosynthetic genes as well as tryptophan decarboxylase ( TDC ) .
[ Sen. 7, subscore: 2.00 ]: Results of TDC overexpression and TDC RNAi plants suggest that TDC plays a rate-limiting role for serotonin accumulation , but the synthesis of serotonin depends on an absolute amount of tryptophan accumulation by the coordinate induction of the tryptophan biosynthetic genes .
[ Sen. 6, subscore: 1.00 ]: The senescence-retarding activity of serotonin is associated with its high antioxidant activity compared to either tryptophan or chlorogenic acid .
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Score: 5.00
Author: Ye M Luo SM Xie JF Li YF Xu T Liu Y Song YY Zhu-Salzman K Zeng RS
Journal: PLoS One Citation: V : 7 P : e36214 Year: 2012 Type: In-Process
Literature: oryza Field: abstract Doc ID: pub22558386 Accession (PMID): 22558386
Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: The abundance of trypsin protease inhibitor ( TrypPI ) , and the enzymatic activities of polyphenol oxidase ( PPO ) and peroxidase ( POD ) were enhanced in response to both LF and BPH infestation , but the activity of lipoxygenase ( LOX ) was only induced by LF .
[ Sen. 8, subscore: 1.00 ]: Silencing OsCOI1 did not alter LF-induced LOX activity and JA content , but it led to a reduction in the TrypPI content , POD and PPO activity by 62 . 3% , 48 . 5% and 27 . 2% , respectively .
[ Sen. 9, subscore: 1.00 ]: In addition , MeJA-induced TrypPI and POD activity were reduced by 57 . 2% and 48 . 2% in OsCOI1 RNAi plants .
[ Sen. 10, subscore: 1.00 ]: These results suggest that OsCOI1 is an indispensable signaling component , controlling JA-regulated defense against chewing insect ( LF ) in rice plants , and COI1 is also required for induction of TrypPI , POD and PPO in rice defense response to LF infestation .
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Score: 4.00
Author: Ansari MM Sridhar R
Journal: Folia Microbiol . ( Praha ) Citation: V : 45 ( 6 ) P : 531-7 Year: Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub11501419 Accession (PMID): 11501419
Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: The isolates metabolized tryptophan through two different routes and the isolates vary in the pathway of tryptophan utilization .
[ Sen. 5, subscore: 1.00 ]: The IAA-positive isolates converted tryptophan to IAA as the end product , whereas the IAA-negative isolates formed anthranilate as an intermediate metabolite and finally produced pyrocatechol via the kynurenine pathway .
[ Sen. 6, subscore: 1.00 ]: Quantification of tryptophan metabolism revealed that the maximum production of IAA and pyrocatechol in culture occurred during 2-d incubation at 30 +/- 2 degrees C
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Score: 4.00
Author: Kim DS Lee IS Jang CS Kang SY Seo YW .
Journal: Plant Cell Rep . Citation: V : 24 ( 6 ) P : 357-65 Year: 2005 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub15776237 Accession (PMID): 15776237
Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: The transcript levels of putative OASA2 , which is one of the key-regulating enzyme subunits in the tryptophan biosynthesis pathway , were studied in the control and 5MT-resistant mutant lines subjected to inhibition by two tryptophan analogs ( 5MT and alphaMT ) and to other abiotic stresses ( ABA , NaCl , and cold ) .
[ Sen. 4, subscore: 1.00 ]: In a kinetic analysis with tryptophan , an anthranilate synthase of the mutant lines was insensitive to feedback inhibition .
[ Sen. 8, subscore: 1.00 ]: Significant increases among the amino acids of the MR lines were observed for tryptophan , phenylalanine , and tyrosine , which had been biosynthesized through the shikimate pathway .
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Score: 4.00
Author: Siriwardana S Nabors MW .
Journal: Citation: V : 73 ( 1 ) P : 142-146 Year: 1983 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub16663163 Accession (PMID): 16663163
Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: High concentrations of tryptophan increased the formation of embryogenic callus in three rice cultivars ( Oryza sativa L Calrose 76 , Pokkali , and IR 36 ) but not in four others ( Mahsuri , Bg 400-1 , H ( 4 ) , and Giza 159 ) .
[ Sen. 5, subscore: 1.00 ]: The best concentration of tryptophan for Pokkali and Calrose 76 was 100 micrograms per milliliter , and for IR 36 , 50 micrograms per milliliter .
[ Sen. 6, subscore: 1.00 ]: Indoleacetic acid at 100 micrograms per milliliter promoted an effect similar to that of tryptophan on Calrose 76 .
[ Sen. 7, subscore: 1.00 ]: The difference between japonica ( Calrose 76 , Giza 159 ) and indica ( Pokkali , IR 36 ) varieties is not the causal factor for the difference in response to tryptophan .
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Score: 4.00
Author: da Silveira Ramos V de Souza Silva G das Gracas Machado Freire M Lima Tavares Machado O Postali Parra JR Rodrigues Macedo ML
Journal: J Agric Food Chem Citation: V : P : Year: 2008 Type: Publisher
Literature: oryza Field: abstract Doc ID: pub18991455 Accession (PMID): 18991455
Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: A novel trypsin inhibitor ( PFTI ) was isolated from Plathymenia foliolosa ( Benth . ) seeds by gel filtration chromatography on a Sephadex G-100 , DEAE-Sepharose , and trypsin-Sepharose columns .
[ Sen. 3, subscore: 1.00 ]: PFTI inhibited bovine trypsin and bovine chymotrypsin with equilibrium dissociation constants ( K i ) of 4 x 10 ( -8 ) and 1 . 4 x 10 ( -6 ) M , respectively .
[ Sen. 10, subscore: 1.00 ]: PFTI showed significant inhibitory activity against trypsin-like proteases present in the larval midguts on A kuehniella and D saccharalis and could suppress the growth of larvae .
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Score: 4.00
Author: Yang Z Xia X Wang X He G
Journal: Z Naturforsch C Citation: V : 65 P : 109-18 Year: 2010 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20355330 Accession (PMID): 20355330
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A reverse transcription-polymerase chain reaction ( RT-PCR ) strategy was used to clone diverse trypsin-like protease gene transcripts from midguts of the brown planthopper Nilaparvata lugens Stal ( Homoptera : Delphacidae ) .
[ Sen. 2, subscore: 1.00 ]: Six individual trypsin-like protease transcripts were identified .
[ Sen. 4, subscore: 1.00 ]: The cDNA contained an 1128-bp open reading frame encoding a putative protein of 375 amino acids with typical features of the trypsin-like protease .
[ Sen. 7, subscore: 1.00 ]: The trypsin-like protease is therefore likely one of the major digestive proteases responsible for protein hydrolysis in N lugens gut , and multiple gene families encoding digestive proteases may help in adaptation of this sap-sucker to different rice varieties .
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Score: 4.00
Author: Kim NS Yu HY Chung ND Shin YJ Kwon TH Yang MS
Journal: Protein Expr Purif Citation: V : 76 P : 121-6 Year: 2011 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20951807 Accession (PMID): 20951807
Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: Proteolytic activity of the rice-derived trypsin was confirmed by gelatin zymogram , and was similar to that of the commercial bovine-produced trypsin .
[ Sen. 1, subscore: 1.00 ]: A synthetic bovine trypsinogen ( sbTrypsinogen ) was synthesized on the basis of rice-optimized codon usage via an overlap PCR strategy , prior to being expressed under the control of the sucrose starvation-inducible rice alpha-amylase 3D ( RAmy3D ) promoter .
[ Sen. 2, subscore: 1.00 ]: Secretion of trypsin into the culture medium was achieved by using the existing signal peptide .
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Score: 4.00
Author: Park S Lee K Kang K Kim YS Lee S Kweon SJ Back K
Journal: Biosci Biotechnol Biochem Citation: V : 74 P : 2352-4 Year: 2010 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub21071852 Accession (PMID): 21071852
Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: We examined to determine whether senescence-induced tryptophan levels are positively associated with levels of glutamine synthetase ( GS1 ) , the initial enzyme in tryptophan biosynthesis .
[ Sen. 3, subscore: 2.00 ]: The transgenic line showed a dramatic decrease in GS1 protein and glutamine content , but the levels of tryptophan and mRNA of the key tryptophan biosynthetic genes upon senescence were comparable to those of the wild type .
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Score: 4.00
Author: Zhang B Zhang DP Zhang WB Wang KM Li G Yao YZ Wu JG Shi CH
Journal: Guang Pu Xue Yu Guang Pu Fen Xi Citation: V : 31 P : 73-6 Year: 2011 Type: In-Process
Literature: oryza Field: abstract Doc ID: pub21428060 Accession (PMID): 21428060
Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: It was concluded that the equation could be directly used to estimate the content of tryptophan in milled rice , and the technology of near infrared reflectance spectroscopy developed in this study could be applied in selecting breeding materials for higher tryptophan content and in quality control during rice processing .
[ Sen. 1, subscore: 1.00 ]: Tryptophan is one of the essential amino acids in human , and also considered as an important limiting amino acid in rice .
[ Sen. 2, subscore: 1.00 ]: Alkali hydrolysis method was used to determine the tryptophan content of 272 representative samples selected from the 1 256 samples in four years .
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Score: 4.00
Author: Sailasuta A Satetasit J Chutmongkonkul M
Journal: Vet Med Int Citation: V : 2011 P : 850568 Year: 2011 Type: PubMed-not-MEDLINE
Literature: oryza Field: abstract Doc ID: pub21918731 Accession (PMID): 21918731
Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: The results showed that 70% of the frogs ( 98/140 ) were infected with 5 species of blood parasites , including a Trypanosoma rotatorium-like organism , Trypanosoma chattoni , Hepatozoon sp . a , Hepatozoon sp . b , and Lankesterella minima .
[ Sen. 5, subscore: 2.00 ]: Histopathological findings associated with the Trypanosoma rotatorium-like organism and Trypanosoma chattoni-infected frogs showed no pathological lesions .
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Score: 4.00
Author: Ge ZY Wan PJ Han ZJ
Journal: Genome Citation: V : 55 P : 281-8 Year: 2012 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub22432944 Accession (PMID): 22432944
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Serine proteinases including trypsins and chymotrypsins play various important roles in insects , including food digestion , immune defense , and zymogen activation .
[ Sen. 3, subscore: 1.00 ]: In this paper , 11 transcripts encoding trypsin and chymotrypsin-like proteins were cloned from the striped rice stem borer , Chilo suppressalis ( Walker ) .
[ Sen. 4, subscore: 1.00 ]: All the predicted proteins share high sequence similarity with known trypsin and chymotrypsin-like proteins from either lepidopterans or dipterans , and most of the proteins have conserved motifs that are characteristics of serine proteinases .
[ Sen. 8, subscore: 1.00 ]: The identification of trypsin and chymotrypsin-like genes in this insect species is the first step towards further comparative studies and for identification of insect-specific proteinase inhibitors , which might be engineered to protect rice plants against the striped rice stem borer , which is one of the destructive pests of rice .
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Score: 4.00
Author: Tashiro M Maki Z
Journal: J Nutr . Sci . Vitaminol . Citation: V : 32 ( 6 ) P : 591-9 Year: 1986 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub3108471 Accession (PMID): 3108471
Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Titrations of the purified enzymes with RBTI showed that bovine , hog , and rat trypsins were powerfully inhibited by the inhibitor , while human trypsin was only weakly inhibited .
[ Sen. 1, subscore: 1.00 ]: The stability and inhibitory specificity of rice bran trypsin inhibitor ( RBTI ) was investigated in an attempt to understand its nutritional significance .
[ Sen. 4, subscore: 1.00 ]: To determine inhibitory specificity , bovine , hog , rat , and human trypsins were used as target enzymes bound to an immobilized RBTI column .
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Score: 4.00
Author: Maki Z Tashiro M
Journal: J Nutr . Sci . Vitaminol . Citation: V : 29 ( 3 ) P : 293-302 Year: 1983 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub6619992 Accession (PMID): 6619992
Matching Sentences:
[ Sen. 3, subscore: 3.00 ]: On the other hand , RBPC possessed the trypsin inhibitor activity corresponding to the complete inhibition of about 6 mg of bovine trypsin per 1 g of dry material The activity was , however , completely destroyed by autoclaving RBPC for 30 min at 121 degrees C In vitro digestion tests showed that RBPC was easily digested by pepsin but was resistant to the attack by trypsin , compared with autoclaved RBPC .
[ Sen. 6, subscore: 1.00 ]: It is presumed that one of the reasons which explains these phenomena is the presence of trypsin inhibitor in RBPC .
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Score: 3.00
Author: Mazumdar-Leighton S Babu CR Bennett J
Journal: Insect Biochem . Mol . Biol . Citation: V : 30 ( 1 ) P : 57-68 Year: 2000 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub10646971 Accession (PMID): 10646971
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The RT-PCR primers encoded the conserved regions around the active site histidine57 and serine195 of Drosophila melanogaster alpha trypsin , including aspartate189 of the specificity pocket .
[ Sen. 5, subscore: 1.00 ]: Sequencing of the 3RACE products indicated that SiP1 , SiP2 and HaP1 encoded trypsin-like serine proteinases , while HaP2 encoded a chymotrypsin-like serine proteinases .
[ Sen. 6, subscore: 1.00 ]: The SiP3 transcript proved to be an abundant 960 nt mRNA encoding a trypsin-like protein in which the active site serine195 was replaced by aspartate .
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Score: 3.00
Author: Foissac X Edwards MG Du JP Gatehouse AM Gatehouse JA .
Journal: Insect Biochem . Mol . Biol . Citation: V : 32 ( 9 ) P : 967-78 Year: 2002 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub12213233 Accession (PMID): 12213233
Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Assays with specific inhibitors suggested that a trypsin-like serine protease was responsible for most of hydrolytic activity against synthetic substrates .
[ Sen. 4, subscore: 1.00 ]: A cDNA library was prepared from RNA extracted from N lugens gut it issue , and screened for protease-encoding sequences . cDNAs for a cathepsin B-like protease and a trypsin-like protease were isolated and fully characterised ; the latter exhibits a novel C-terminal region and an unusual activation mechanism , and represents a small gene family .
[ Sen. 5, subscore: 1.00 ]: Soya bean Kunitz trypsin inhibitor ( SKTI ) is an effective inhibitor of protein hydrolysis by N lugens gut extracts in vitro , explaining why transgenic rice plants expressing this protein are partially resistant to the insect ( Mol . Breed . 5 ( 1999 ) 1 ) .
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Score: 3.00
Author: Alfonso-Rub J Ortego F Castaera P Carbonero P Daz I
Journal: Transgenic Res . Citation: V : 12 ( 1 ) P : 23-31 Year: 2003 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub12650522 Accession (PMID): 12650522
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Indica and japonica rice ( Oryza sativa L ) plants were transformed by particle bombardment with the Itr1 gene encoding the barley trypsin inhibitor BTI-CMe , under the control of its own promoter that confers endosperm specificity , and the maize ubiquitin promoter .
[ Sen. 4, subscore: 1.00 ]: The functional integrity of BTI-CMe was confirmed by trypsin activity assays in liquid media and by activity staining gels , performed with seed extracts .
[ Sen. 5, subscore: 1.00 ]: The significant reduction of the survival rate of the rice weevil ( Sitophilus oryzae , Coleoptera : Curculionidae ) reared on homozygous transgenic indica and japonica rice seeds expressing the BTI-CMe , compared to non-transformed controls , and the decrease in the trypsin-like activity of insect crude midgut extracts , confirmed the utility of this proteinase inhibitor gene for the control of important storage pests .
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Score: 3.00
Author: Casellas F Guarner L Antoln M Malagelada JR .
Journal: Pancreas Citation: V : 29 ( 4 ) P : 306-10 Year: 2004 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub15502647 Accession (PMID): 15502647
Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: METHODS : Ten patients with clinically suspected pancreatic insufficiency confirmed with a duodenal perfusion technique and measurement of trypsin and lipase output after cerulein stimulation were included .
[ Sen. 9, subscore: 1.00 ]: Stimulated duodenal output of lipase and trypsin was abnormally low in all patients ( median release , 1 . 7 ; range , 0 . 5-4 . 5 and 0 . 8 , range , 0 . 1-11 . 3 KU/h , respectively ) .
[ Sen. 11, subscore: 1.00 ]: Correlation between H2-rice breath test and basal or stimulated duodenal output of lipase and trypsin was not significant .
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Score: 3.00
Author: Sugita K Endo-Kasahara S Tada Y Lijun Y Yasuda H Hayashi Y Jomori T Ebinuma H Takaiwa F
Journal: FEBS Lett . Citation: V : 579 ( 5 ) P : 1085-8 Year: 2005 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub15710395 Accession (PMID): 15710395
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: We produced large amounts of a GLP-1 analogue , [ Ser8 , Gln26 , Asp34 ] -GLP-1 , which is resistant to trypsin-digestion , as part of a chimeric rice seed storage protein , a 26 kDa globulin , in genetically modified rice seeds .
[ Sen. 3, subscore: 1.00 ]: Junction sites between GLP-1 analogue and globulin were replaced by tryptic cleavage sites .
[ Sen. 5, subscore: 1.00 ]: We found that GLP-1 analogue derived from trypsin-digested genetically modified rice seeds stimulated insulin secretion from a mouse pancreatic beta-cell line , MIN6 .
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Score: 3.00
Author: Kanno T Komatsu A Kasai K Dubouzet JG Sakurai M Ikejiri-Kanno Y Wakasa K Tozawa Y
Journal: Plant Physiol . Citation: V : 138 ( 4 ) P : 2260-8 Year: 2005 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub16040654 Accession (PMID): 16040654
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Twenty-four amino acid residues , predicted as putative tryptophan binding sites or their proximal regions in the OASA2 sequence , were selected and 36 mutant OASA2 genes were constructed by PCR-based site-directed mutagenesis .
[ Sen. 4, subscore: 1.00 ]: Enzymatic functions of the mutant proteins were simultaneously examined , and we found six mutants with elevated catalytic activity and five mutants with enhanced tolerance to feedback inhibition by tryptophan .
[ Sen. 7, subscore: 1.00 ]: The free tryptophan content of mutant rice calli expressing OASA2 enzyme with a double mutation was 30-fold of that of untransformed calli .
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Score: 3.00
Author: Yamaya T Solomonson LP Oaks A
Journal: Citation: V : 65 ( 1 ) P : 146-150 Year: 1980 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub16661131 Accession (PMID): 16661131
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: When nitrate reductase ( NR ) purified from Chlorella was incubated with NR-inactivating proteins purified from corn roots and rice cell suspension cultures or with trypsin there was a loss in NADH-NR and NADH cytochrome c reductase ( NADH-CR ) activities with time whereas the reduced methylviologen NR ( MV-NR ) remained active .
[ Sen. 4, subscore: 1.00 ]: Incubation with trypsin also caused a change in the NR protein migration pattern ( R ( F ) value of 0 . 20 ) .
[ Sen. 6, subscore: 1.00 ]: Thus , the corn inactivator degrades NR in a fashion similar to but not identical with trypsin .
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Score: 3.00
Author: Gannon JT Linke HA .
Journal: Int . J Parasitol . Citation: V : 21 ( 4 ) P : 403-7 Year: 1991 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub1717390 Accession (PMID): 1717390
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Diamonds TYI-S-33 ( Trypticase-Yeast Extract-Iron-Serum ) medium was used as the basis for a new antibiotic-free medium for xenic growth of Entamoeba gingivalis .
[ Sen. 4, subscore: 1.00 ]: The changes included : ( a ) deletion of L-cysteine . HCl and thioctic acid , ( b ) substitution of glucose for dextran I ( mol . wt 185 , 000 ) or rice starch , ( c ) reduction of concentrations of tryptone ( 2 . 5 g l-1 ) , yeast extract ( 1 . 25 g l-1 ) and dextran I ( 1 g l-1 ) , ( d ) increased concentration of ferric ammonium citrate ( 0 . 2 g l-1 ) , and ( e ) addition of gastric mucin ( 2 . 4 g l-1 ) .
[ Sen. 6, subscore: 1.00 ]: This new antibiotic-free medium significantly increased E gingivalis growth ( 16-20 E gingivalis trophozoites observed per field ) as compared to growth in Diamonds TYSGM-9 ( Trypticase-Yeast Extract-Serum-Gastric Mucin ) medium ( six to 10 E gingivalis trophozoites observed per field ) .
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Score: 3.00
Author: Jain NK Boivin M Zinsmeister AR DiMagno EP .
Journal: Pancreas Citation: V : 6 ( 5 ) P : 495-505 Year: 1991 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub1719522 Accession (PMID): 1719522
Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Postprandially , we measured outputs of amylase , trypsin , and bile acids in the duodenum , the amount of carbohydrate in the ileum , and gastric emptying .
[ Sen. 7, subscore: 1.00 ]: However , as the amount of unabsorbed carbohydrate in the ileum increased , the ratio of amylase to trypsin secretion increased ( p less than 0 . 005 ) .
[ Sen. 8, subscore: 1.00 ]: Postprandially carbohydrate in the ileum induces changes of upper-gut function that should increase digestion and absorption of carbohydrate since gastric emptying ( and delivery of carbohydrate to the duodenum ) slows and pancreatic amylase secretion increases relative to trypsin secretion and gastric emptying .
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Score: 3.00
Author: Ishihara A Hashimoto Y Tanaka C Dubouzet JG Nakao T Matsuda F Nishioka T Miyagawa H Wakasa K
Journal: Plant J Citation: V : 54 P : 481-95 Year: 2008 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub18266919 Accession (PMID): 18266919
Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: Serotonin accumulation was preceded by a transient increase in the tryptamine content and by marked activation of Trp decarboxylase , indicating that enhanced Trp production is linked to the formation of serotonin from Trp via tryptamine .
[ Sen. 1, subscore: 1.00 ]: The upregulation of the tryptophan ( Trp ) pathway in rice leaves infected by Bipolaris oryzae was indicated by : ( i ) enhanced enzyme activity of anthranilate synthase ( AS ) , which regulates metabolic flux in the Trp pathway ; ( ii ) elevated levels of the AS ( OASA2 , OASB1 , and OASB2 ) transcripts ; and ( iii ) increases in the contents of anthranilate , indole , and Trp .
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Score: 3.00
Author: Lingaraju MH Gowda LR
Journal: Biochim Biophys Acta Citation: V : 1784 P : 850-5 Year: 2008 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub18359299 Accession (PMID): 18359299
Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: A potent Kunitz type trypsin inhibitor ( ESTI ) was purified to homogeneity from Entada scandens seeds by sequential ammonium sulfate precipitation , affinity chromatography on trypsin-Sepharose and DEAE-Sephacel ion-exchange chromatography .
[ Sen. 8, subscore: 1.00 ]: ESTI inhibited bovine trypsin with a stoichiometry of 1 : 1 and the apparent K ( i ) was 4 . 9 x 10 ( -9 ) M In vitro assay showed that ESTI inhibited the midgut proteinase of the fifth instar larvae of Rice moth ( Corcyra cephalonica ) with an IC ( 50 ) of 26 . 4+/-0 . 01 nM .
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Score: 3.00
Author: Ramos Vda S Freire MG Parra JR Macedo ML
Journal: Comp Biochem Physiol A Mol Integr Physiol Citation: V : 152 P : 255-61 Year: 2009 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub19007900 Accession (PMID): 19007900
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Plants synthesize a variety of molecules , including trypsin inhibitors , to defend themselves against attack by insects .
[ Sen. 3, subscore: 1.00 ]: In this study , a trypsin inhibitor ( PFTI ) was purified from Plathymenia foliolosa ( Benth . ) seeds and was tested for insect growth regulatory effect .
[ Sen. 5, subscore: 1.00 ]: The results from dietary utilization experiments with A kuehniella larvae showed a reduction in efficiency of conversion of ingested food and digested food , and an increase in approximate digestibility and metabolic cost The level of trypsin was significantly decreased in larval midgut and increased in the feces of larvae reared on a diet containing 0 . 7% PFTI .
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Score: 3.00
Author: Yin F Zhang Z Huang J Yin Y
Journal: Br J Nutr Citation: V : 103 P : 1404-12 Year: 2010 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20102672 Accession (PMID): 20102672
Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: The concentrations of nutritionally indispensable AA , including arginine , cystine , histidine , isoleucine , leucine , methionine , phenylalanine , threonine , tryptophan , tyrosine and valine at 09 . 30 hours and arginine , cystine , histidine , isoleucine , methionine , phenylalanine , threonine , tryptophan , tyrosine and valine at 13 . 30 hours in the SRS group were higher than in the MS group ( P < 0 . 05 ) ; all nutritionally indispensable AA in the SRS group were higher than in the RS group at 09 . 30 and 13 . 30 hours ( P < 0 . 05 ) , respectively .
[ Sen. 6, subscore: 1.00 ]: The digestibilities of isoleucine , leucine , methionine , phenylalanine , threonine , tryptophan , valine , alanine , aspartate and serine in the SRS group were higher than in the MS group ( P < 0 . 05 ) , and all nutritionally indispensable and dispensable AA in the SRS group were higher when compared with those in the RS group ( P < 0 . 05 ) .
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Score: 3.00
Author: Fujiwara T Maisonneuve S Isshiki M Mizutani M Chen L Wong HL Kawasaki T Shimamoto K
Journal: J Biol Chem Citation: V : 285 P : 11308-13 Year: 2010 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20150424 Accession (PMID): 20150424
Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: A recombinant SL protein exhibited tryptamine 5-hydroxylase enzyme activity and catalyzed the conversion of tryptamine to serotonin .
[ Sen. 3, subscore: 1.00 ]: The rice sekiguchi lesion ( sl ) mutants accumulate tryptamine , a candidate substrate for serotonin biosynthesis .
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Score: 3.00
Author: Giordano PC Martinez HD Iglesias AA Beccaria AJ Goicoechea HC
Journal: Bioresour Technol Citation: V : 101 P : 7537-7544 Year: 2010 Type: Publisher
Literature: oryza Field: abstract Doc ID: pub20576568 Accession (PMID): 20576568
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Production of recombinant Oryza sativa non-symbiotic hemoglobin 1 ( OsHb1 ) by Escherichia coli was maximized in shake-flask cultures in media containing tryptone , yeast extract , sodium chloride and byproduct glycerol from biodiesel production .
[ Sen. 4, subscore: 1.00 ]: The optimal conditions were ( gL ( -1 ) ) , tryptone , 42 . 69 ; yeast extract , 20 . 11 ; sodium chloride , 17 . 77 ; and byproduct glycerol , 0 . 33 .
[ Sen. 6, subscore: 1.00 ]: Although the concentrations of tryptone , yeast extract and sodium chloride are relatively high , the increase in the yield with respect to biomass formed ( Y ( P/X ) ) overcomes this disadvantage .
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Score: 3.00
Author: Giordano PC Martinez HD Iglesias AA Beccaria AJ Goicoechea HC
Journal: Bioresour Technol Citation: V : 101 P : 7537-44 Year: 2010 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20578289 Accession (PMID): 20578289
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Production of recombinant Oryza sativa non-symbiotic hemoglobin 1 ( OsHb1 ) by Escherichia coli was maximized in shake-flask cultures in media containing tryptone , yeast extract , sodium chloride and byproduct glycerol from biodiesel production .
[ Sen. 4, subscore: 1.00 ]: The optimal conditions were ( g L ( -1 ) ) , tryptone , 42 . 69 ; yeast extract , 20 . 11 ; sodium chloride , 17 . 77 ; and byproduct glycerol , 0 . 33 .
[ Sen. 6, subscore: 1.00 ]: Although the concentrations of tryptone , yeast extract and sodium chloride are relatively high , the increase in the yield with respect to biomass formed ( Y ( P/X ) ) overcomes this disadvantage .
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Score: 3.00
Author: Zibaee A Bandani AR Fazeli-Dinan M Zibaee I Sendi JJ Maleki FA
Journal: Arch Insect Biochem Physiol Citation: V : P : Year: 2011 Type: Publisher
Literature: oryza Field: abstract Doc ID: pub21678486 Accession (PMID): 21678486
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice green semi looper , Naranga aenescens Moore ( Lepidoptera : Noctuidae ) causes severe damage to rice fields in Eastern Asia and Middle East We demonstrate that two types of serine proteases are active in the midgut of the third instar larvae of N aenescens , but trypsin-like proteases are considerably more active than chymotrypsin-like proteases .
[ Sen. 2, subscore: 1.00 ]: To develop better control strategies , purification and biochemical characterization of a major trypsin-like digestive protease from the midgut of the third instar larvae of N aenescens was achieved by gel filtration and anion exchange chromatography .
[ Sen. 5, subscore: 1.00 ]: Divalent cations , especially Ca ( 2+ ) , Mg ( 2+ ) , and Cu ( 2+ ) , increased the enzyme activity and synthetic inhibitors that target trypsin-like activity caused a significant reduction in caseinolytic activity .
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Score: 3.00
Author: Park S Lee K Kim YS Back K
Journal: J Pineal Res Citation: V : 52 P : 211-6 Year: 2012 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub21884550 Accession (PMID): 21884550
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Melatonin biosynthesis was examined in Sekiguchi mutant rice lacking functional tryptamine 5-hydroxylase ( T5H ) activity , which is the terminal enzyme for serotonin biosynthesis in rice .
[ Sen. 2, subscore: 1.00 ]: During senescence process , the leaves of Sekiguchi mutant rice produced more tryptamine and N-acetyltryptamine compared with the wild-type Asahi leaves .
[ Sen. 5, subscore: 1.00 ]: In contrast , a large amount of N-acetyltryptamine was accumulated in Sekiguchi leaves , indicating that tryptamine was efficiently utilized as substrate by the rice arylalkylamine N-acetyltransferase enzyme .
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Score: 3.00
Author: Zhuang X Fiesselmann A Zhao N Chen H Frey M Chen F
Journal: Phytochemistry Citation: V : 73 P : 15-22 Year: 2012 Type: In-Data-Review
Literature: oryza Field: abstract Doc ID: pub22000657 Accession (PMID): 22000657
Matching Sentences:
[ Sen. 3, subscore: 3.00 ]: The alpha-subunit of tryptophan synthase ( TSA ) is the IGL that catalyses the conversion of indole-3-glycerol phosphate to indole in the alpha-reaction of tryptophan synthesis ; however , TSA is only active in the complex with the beta-subunit of tryptophan synthase and is not capable of producing free indole .
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Score: 3.00
Author: Abu-Zaitoon YM Bennett K Normanly J Nonhebel HM
Journal: Physiol Plant Citation: V : P : Year: 2012 Type: Publisher
Literature: oryza Field: abstract Doc ID: pub22582989 Accession (PMID): 22582989
Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: Expression of tryptophan decarboxylase in developing rice grains did not correlate with IAA levels , indicating that tryptamine is unlikely to be important for IAA synthesis in this system .
[ Sen. 5, subscore: 1.00 ]: These results confirm the importance of the tryptophan aminotransferase/YUCCA pathway in this system .
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Score: 3.00
Author: Song T Ma CY Chu IK Siu CK Laskin J
Journal: J Phys Chem A Citation: V : P : Year: 2012 Type: Publisher
Literature: oryza Field: abstract Doc ID: pub22697598 Accession (PMID): 22697598
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The C ( beta ) -C ( gamma ) bond cleavage of a C-terminal decarboxylated tryptophan residue ( [ M - CO ( 2 ) ] ( *+ ) ) can generate [ M - CO ( 2 ) - 116 ] ( + ) , [ M - CO ( 2 ) - 117 ] ( *+ ) , and [ 1H-indole ] ( *+ ) ( m/z 117 ) species as possible product ions .
[ Sen. 4, subscore: 1.00 ]: Formation of such a proton-bound dimer is facile via a protonation of the tryptophan gamma-carbon atom as suggested by density functional theory ( DFT ) calculations .
[ Sen. 6, subscore: 1.00 ]: The C ( beta ) -C ( gamma ) bond cleavage of a tryptophan residue also can occur directly from peptide radical cations containing a basic residue .
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Score: 3.00
Author: Tashiro M Maki Z
Journal: J Nutr . Sci . Vitaminol . Citation: V : 25 ( 3 ) P : 255-64 Year: Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub501453 Accession (PMID): 501453
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A trypsin inhibitor was isolated and purified from the bran of rice , Oryza sativa , by extraction with 1% sodium chloride , heat treatment , ammonium sulfate precipitation , ion-exchange chromatography on a CM-Sephadex C-25 and gel filtration on a Sephadex G-75 .
[ Sen. 3, subscore: 1.00 ]: Rice bran trypsin inhibitor ( RBTI ) had a molecular weight of about 14 , 500 and an isoelectric point of 8 . 07 .
[ Sen. 5, subscore: 1.00 ]: BRTI inhibited bovine trypsin at an inhibitor-enzyme molar ratio of 1 : 1 . 6 .
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Score: 3.00
Author: Clark HE Bailey LB Brewer MF .
Journal: Am . J Clin . Nutr . Citation: V : 30 ( 5 ) P : 674-80 Year: 1977 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub855810 Accession (PMID): 855810
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Two levels of lysine , 900 and 1800 mg , and three of tryptophan , 260 , 390 , and 520 mg , were tested .
[ Sen. 3, subscore: 1.00 ]: Lysine exerted a significant effect ( P less than 0 . 05 ) on nitrogen retention but tryptophan did not .
[ Sen. 4, subscore: 1.00 ]: When consumed in conjunction with at least twice the reported minimal requirements of other essential amino acids , 900 mg of lysine induced mean daily balances of 0 . 15 +/- 0 . 18 , 0 . 19 +/- 0 . 14 , and 0 . 22 +/- 0 . 21 g , respectively , in response to the three levels of tryptophan ; and 1800 mg of lysine caused retentions of 0 . 75 +/- 0 . 14 , 0 . 77 +/- 0 . 21 and 0 . 71 +/- 0 . 15 g .
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