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Score: 21.00 | Title: Incorporation and distribution of tritium in rats exposed to tritiated rice or tritiated soybean .
| Journal: J Radiat . Res . Citation: V : 33 ( 4 ) P : 309-18 Year: 1992 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub1293301 Accession (PMID): 1293301 | Abstract: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding .
Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions .
All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop .
The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue .
The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water .
After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water .
At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water . | Matching Sentences: [ Sen. 5, subscore: 5.00 ]: The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water . [ Sen. 6, subscore: 5.00 ]: After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water . [ Sen. 1, subscore: 3.00 ]: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding . [ Sen. 2, subscore: 2.00 ]: Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions . [ Sen. 3, subscore: 2.00 ]: All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop . [ Sen. 4, subscore: 2.00 ]: The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue . [ Sen. 7, subscore: 2.00 ]: At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water .
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Score: 16.00 | Title: TriFLDB : A Database of Clustered Full-Length Coding Sequences from Triticeae with Applications to Comparative Grass Genomics .
| Journal: Plant Physiol Citation: V : P : Year: 2009 Type: Publisher | Literature: oryza Field: abstract Doc ID: pub19448038 Accession (PMID): 19448038 | Abstract: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features .
TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries .
Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan .
The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences .
The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries .
To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) .
These relational data are searchable from the search interfaces of both databases .
The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible .
This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics .
TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ .
| Matching Sentences: [ Sen. 1, subscore: 4.00 ]: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features . [ Sen. 2, subscore: 3.00 ]: TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries . [ Sen. 6, subscore: 3.00 ]: To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) . [ Sen. 5, subscore: 2.00 ]: The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries . [ Sen. 4, subscore: 1.00 ]: The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences . [ Sen. 8, subscore: 1.00 ]: The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible . [ Sen. 9, subscore: 1.00 ]: This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics . [ Sen. 10, subscore: 1.00 ]: TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ .
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Score: 14.00 | Title: Growth-inhibitory and cell cycle-arresting properties of the rice bran constituent tricin in human-derived breast cancer cells in vitro and in nude mice in vivo .
| Journal: Br .
J Cancer Citation: V : 91 ( 7 ) P : 1364-71 Year: 2004 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub15316567 Accession (PMID): 15316567 | Abstract: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations .
As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo .
Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells .
Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining .
In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development .
Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively .
Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice .
The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls .
These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour .
While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . | Matching Sentences: [ Sen. 2, subscore: 3.00 ]: As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . [ Sen. 3, subscore: 2.00 ]: Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . [ Sen. 8, subscore: 2.00 ]: The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . [ Sen. 1, subscore: 1.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . [ Sen. 4, subscore: 1.00 ]: Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . [ Sen. 5, subscore: 1.00 ]: In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . [ Sen. 6, subscore: 1.00 ]: Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . [ Sen. 7, subscore: 1.00 ]: Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . [ Sen. 9, subscore: 1.00 ]: These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . [ Sen. 10, subscore: 1.00 ]: While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious .
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Score: 13.00 | Title: Isolation and characterization of wheat triticin cDNA revealing a unique lysine-rich repetitive domain .
| Journal: Plant Mol . Biol . Citation: V : 22 ( 2 ) P : 227-37 Year: 1993 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub8507826 Accession (PMID): 8507826 | Abstract: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 .
The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies .
Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones .
This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin .
The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B .
The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds .
A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes .
Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) .
There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing .
The maximum mRNA accumulation occurred between 17 and 20 DAA .
These observations conform closely with the published data on triticin protein accumulation during grain development .
| Matching Sentences: [ Sen. 4, subscore: 3.00 ]: This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . [ Sen. 5, subscore: 2.00 ]: The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . [ Sen. 6, subscore: 2.00 ]: The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . [ Sen. 1, subscore: 1.00 ]: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 . [ Sen. 3, subscore: 1.00 ]: Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . [ Sen. 7, subscore: 1.00 ]: A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . [ Sen. 8, subscore: 1.00 ]: Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . [ Sen. 9, subscore: 1.00 ]: There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . [ Sen. 11, subscore: 1.00 ]: These observations conform closely with the published data on triticin protein accumulation during grain development .
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Score: 12.00 | Title: Flavor production from edible oils and their constituents by Penicillium corylophilum .
| Journal: Shokuhin Eiseigaku Zasshi Citation: V : 43 ( 3 ) P : 160-4 Year: 2002 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12238154 Accession (PMID): 12238154 | Abstract: Production of volatile substances from edible oils and their constituents by Penicillium corylophilum was studied to clarify the mechanism of flavor production from a non-stick oil by the organism in a rice cake system .
First , edible oils from plant and animal origins were tested for flavor production .
Among the oils tested , coconut oil was the only one from which the flavor was produced .
Second , triacylglycerols consisting of fatty acids with various lengths of carbon chain ( C6 to C13 ) were studied for flavor production .
Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 .
The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols .
Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested .
Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids .
Third , fatty acids with various lengths of carbon chain ( C7 to C15 ) were studied for flavor production .
Among the fatty acids tested , flavors were produced from decanoic ( C10 ) and undecanoic ( C11 ) acids only .
The flavors also consisted of methylketones and secondary alcohols one carbon shorter than the precursor fatty acids .
Fatty acids with short carbon chains ( C7 to C9 ) completely inhibited the mould growth .
Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) .
It was also found that the non-stick oil and coconut oil contain tricaprylin and tridecanoin as triacylglycerols and decanoic acid as fatty acid .
| Matching Sentences: [ Sen. 7, subscore: 4.00 ]: Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested . [ Sen. 14, subscore: 3.00 ]: It was also found that the non-stick oil and coconut oil contain tricaprylin and tridecanoin as triacylglycerols and decanoic acid as fatty acid . [ Sen. 4, subscore: 1.00 ]: Second , triacylglycerols consisting of fatty acids with various lengths of carbon chain ( C6 to C13 ) were studied for flavor production . [ Sen. 5, subscore: 1.00 ]: Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 . [ Sen. 6, subscore: 1.00 ]: The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols . [ Sen. 8, subscore: 1.00 ]: Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids . [ Sen. 13, subscore: 1.00 ]: Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) .
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