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Score: 21.00 | Title: Incorporation and distribution of tritium in rats exposed to tritiated rice or tritiated soybean .
| Journal: J Radiat . Res . Citation: V : 33 ( 4 ) P : 309-18 Year: 1992 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub1293301 Accession (PMID): 1293301 | Abstract: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding .
Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions .
All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop .
The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue .
The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water .
After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water .
At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water . | Matching Sentences: [ Sen. 5, subscore: 5.00 ]: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding . Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions . All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop . The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue . The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water . After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water . At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water . [ Sen. 6, subscore: 5.00 ]: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding . Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions . All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop . The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue . The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water . After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water . At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water . [ Sen. 1, subscore: 3.00 ]: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding . Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions . All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop . The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue . The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water . After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water . At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water . [ Sen. 2, subscore: 2.00 ]: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding . Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions . All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop . The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue . The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water . After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water . At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water . [ Sen. 3, subscore: 2.00 ]: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding . Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions . All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop . The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue . The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water . After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water . At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water . [ Sen. 4, subscore: 2.00 ]: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding . Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions . All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop . The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue . The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water . After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water . At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water . [ Sen. 7, subscore: 2.00 ]: The incorporation and distribution of tritium were examined in rats exposed to tritiated rice or tritiated soybean by single ingestion or continuous feeding . Results were compared with those for tritiated wheat and tritiated water in a previous study done under the same experimental conditions . All the tritiated crops examined were more efficiently incorporated into rat it issues than was tritiated water , the extent of incorporation depending on the kind of crop . The differences in incorporation were clear in organically bound tritium determined as tritium in dried it issue . The respective concentrations of organically bound tritium after a single ingestion of tritiated rice , tritiated wheat or tritiated soybean were about 10-20 , 20-30 and 25-60 times higher than after the ingestion of tritiated water . After continuous feeding for 22 days with tritiated rice , tritiated wheat or tritiated soybean , the respective concentrations of organically bound tritium were 5-8 , 6-11 and 10-25 times the values after continuous ingestion of tritiated water . At the end of continuous ingestion , the radiation dose rates to almost of the it issues from all three tritiated crops were estimated to be 2-3 times that for tritiated water .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 16.00 | Title: TriFLDB : A Database of Clustered Full-Length Coding Sequences from Triticeae with Applications to Comparative Grass Genomics .
| Journal: Plant Physiol Citation: V : P : Year: 2009 Type: Publisher | Literature: oryza Field: abstract Doc ID: pub19448038 Accession (PMID): 19448038 | Abstract: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features .
TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries .
Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan .
The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences .
The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries .
To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) .
These relational data are searchable from the search interfaces of both databases .
The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible .
This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics .
TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ .
| Matching Sentences: [ Sen. 1, subscore: 4.00 ]: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features . TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries . Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan . The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences . The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries . To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) . These relational data are searchable from the search interfaces of both databases . The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible . This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics . TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ . [ Sen. 2, subscore: 3.00 ]: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features . TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries . Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan . The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences . The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries . To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) . These relational data are searchable from the search interfaces of both databases . The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible . This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics . TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ . [ Sen. 6, subscore: 3.00 ]: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features . TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries . Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan . The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences . The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries . To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) . These relational data are searchable from the search interfaces of both databases . The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible . This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics . TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ . [ Sen. 5, subscore: 2.00 ]: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features . TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries . Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan . The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences . The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries . To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) . These relational data are searchable from the search interfaces of both databases . The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible . This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics . TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ . [ Sen. 4, subscore: 1.00 ]: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features . TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries . Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan . The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences . The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries . To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) . These relational data are searchable from the search interfaces of both databases . The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible . This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics . TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ . [ Sen. 8, subscore: 1.00 ]: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features . TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries . Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan . The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences . The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries . To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) . These relational data are searchable from the search interfaces of both databases . The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible . This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics . TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ . [ Sen. 9, subscore: 1.00 ]: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features . TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries . Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan . The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences . The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries . To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) . These relational data are searchable from the search interfaces of both databases . The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible . This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics . TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ . [ Sen. 10, subscore: 1.00 ]: The Triticeae Full-Length Coding Sequence ( CDS ) Database ( TriFLDB ) contains available information regarding full-length CDSs of the Triticeae crops wheat ( Triticum aestivum ) and barley ( Hordeum vulgare ) and includes functional annotations and comparative genomics features . TriFLDB provides a search interface using keywords for gene function and related Gene Ontology ( GO ) terms and a similarity search for DNA and deduced translated amino acid sequences to access annotations of Triticeae full-length CDS ( TriFLCDS ) entries . Annotations consist of similarity search results against several sequence databases and domain structure predictions by InterProScan . The deduced amino acid sequences in TriFLDB are grouped with the proteome datasets for Arabidopsis , rice , and sorghum by hierarchical clustering in stepwise thresholds of sequence identity , providing hierarchical clustering results based on full-length protein sequences . The database also provides sequence similarity results based on comparative mapping of TriFLCDSs onto the rice and sorghum genome sequences , which together with current annotations can be used to predict gene structures for TriFLCDS entries . To provide the possible genetic locations of full-length CDSs , TriFLCDS entries are also assigned to the genetically mapped cDNA sequences of barley and diploid wheat , which are currently accommodated in TriMEDB ( Triticeae Mapped EST Database ) . These relational data are searchable from the search interfaces of both databases . The current TriFLDB contains 15 , 871 full-length CDSs from barley and wheat and includes putative full-length cDNAs for barley and wheat , which are publicly accessible . This informative content provides an informatics gateway for Triticeae genomics and grass comparative genomics . TriFLDB is publicly available at http : //TriFLDB . psc . riken . jp/ .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 14.00 | Title: Growth-inhibitory and cell cycle-arresting properties of the rice bran constituent tricin in human-derived breast cancer cells in vitro and in nude mice in vivo .
| Journal: Br .
J Cancer Citation: V : 91 ( 7 ) P : 1364-71 Year: 2004 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub15316567 Accession (PMID): 15316567 | Abstract: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations .
As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo .
Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells .
Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining .
In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development .
Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively .
Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice .
The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls .
These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour .
While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . | Matching Sentences: [ Sen. 2, subscore: 3.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . [ Sen. 3, subscore: 2.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . [ Sen. 8, subscore: 2.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . [ Sen. 1, subscore: 1.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . [ Sen. 4, subscore: 1.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . [ Sen. 5, subscore: 1.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . [ Sen. 6, subscore: 1.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . [ Sen. 7, subscore: 1.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . [ Sen. 9, subscore: 1.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious . [ Sen. 10, subscore: 1.00 ]: Tricin , a flavone found in rice bran , inhibits the growth of human-derived malignant MDA-MB-468 breast tumour cells at submicromolar concentrations . As part of the exploration of tricin as a potential cancer chemopreventive agent , we investigated the duration and cell cycle specificity of growth inhibition elicited by tricin in vitro and the effect of tricin on the development of MDA-MB-468 tumours grown in immune-compromised MF-1 mice in vivo . Preincubation of MDA-MB-468 cells with tricin ( 1-40 microM ) for 72 h compromised cell growth after tricin removal , and such irreversibility was not observed in human breast-derived nonmalignant HBL-100 cells . Tricin ( >/=5 microM ) arrested MDA-MB-468 cells in the G2/M phase of the cell cycle without inducing apoptosis as adjudged by annexin V staining . In nude mice consumption of tricin with the diet ( 0 . 2% , w w ( -1 ) ) from 1 week prior to MDA-MB-468 cell implantation failed to impede tumour development . Steady-state levels of tricin in plasma , breast tumour it issue and intestinal mucosa , as measured by HPLC , were 0 . 13 microM and 0 . 11 and 63 nmol g ( -1 ) , respectively . Cells were exposed to tricin ( 0 . 11 , 1 . 1 or 11 microM ) in vitro for 72 h and then implanted into mice . The volume of tumours in animals bearing cells pre-exposed to 11 microM tricin was less than a third of that in mice with control cells , while tumours from cells incubated with 0 . 1 or 1 . 1 microM tricin were indistinguishable from controls . These results suggest that the potent breast tumour cell growth-inhibitory activity of tricin in vitro does not directly translate into activity in the nude mouse bearing the MDA MB-468 tumour . While the results do not support the notion that tricin is a promising candidate for breast cancer chemoprevention , its high levels in the gastrointestinal tract after dietary intake render exploration of its ability to prevent colorectal carcinogenesis propitious .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 13.00 | Title: Isolation and characterization of wheat triticin cDNA revealing a unique lysine-rich repetitive domain .
| Journal: Plant Mol . Biol . Citation: V : 22 ( 2 ) P : 227-37 Year: 1993 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub8507826 Accession (PMID): 8507826 | Abstract: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 .
The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies .
Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones .
This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin .
The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B .
The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds .
A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes .
Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) .
There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing .
The maximum mRNA accumulation occurred between 17 and 20 DAA .
These observations conform closely with the published data on triticin protein accumulation during grain development .
| Matching Sentences: [ Sen. 4, subscore: 3.00 ]: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 . The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies . Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . The maximum mRNA accumulation occurred between 17 and 20 DAA . These observations conform closely with the published data on triticin protein accumulation during grain development . [ Sen. 5, subscore: 2.00 ]: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 . The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies . Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . The maximum mRNA accumulation occurred between 17 and 20 DAA . These observations conform closely with the published data on triticin protein accumulation during grain development . [ Sen. 6, subscore: 2.00 ]: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 . The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies . Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . The maximum mRNA accumulation occurred between 17 and 20 DAA . These observations conform closely with the published data on triticin protein accumulation during grain development . [ Sen. 1, subscore: 1.00 ]: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 . The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies . Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . The maximum mRNA accumulation occurred between 17 and 20 DAA . [ Sen. 3, subscore: 1.00 ]: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 . The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies . Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . The maximum mRNA accumulation occurred between 17 and 20 DAA . These observations conform closely with the published data on triticin protein accumulation during grain development . [ Sen. 7, subscore: 1.00 ]: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 . The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies . Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . The maximum mRNA accumulation occurred between 17 and 20 DAA . These observations conform closely with the published data on triticin protein accumulation during grain development . [ Sen. 8, subscore: 1.00 ]: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 . The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies . Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . The maximum mRNA accumulation occurred between 17 and 20 DAA . These observations conform closely with the published data on triticin protein accumulation during grain development . [ Sen. 9, subscore: 1.00 ]: Polyclonal antibodies were raised against a purified 22 kDa triticin polypeptide ( delta ) and were used to screen a wheat seed cDNA library in the Escherichia coli expression vector lambda gt11 . The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies . Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . The maximum mRNA accumulation occurred between 17 and 20 DAA . These observations conform closely with the published data on triticin protein accumulation during grain development . [ Sen. 11, subscore: 1.00 ]: The isolated cDNA clones were grouped into three families based on their cross-hybridization reactions in DNA dot-blot studies . Southern blots of genomic DNAs extracted from ditelocentric and nullisomic-tetrasomic lines of Chinese Spring wheat , probes with the excised cDNA inserts , indicated that one of the three families ( 9 clones ) had triticin clones . This was finally confirmed by comparing the predicted amino acid sequences of two of these clones ( lambda Tri-12 , lambda Tri-25 ) with the published tryptic peptide sequences of triticin . The Southern blots also showed that there is at least one triticin gene located on the short arm of each of the homoeologous group 1 chromosomes ( 1A , 1B , 1D ) , although till now no triticin protein product has been identified for the chromosome 1B . The nucleotide sequence of the largest triticin cDNA clone lambda Tri-25 ( 1567 bp ) is presented here , and its predicted amino acid sequence shows strong homology with the legumin-like proteins of oats ( 12S globulin ) , rice ( glutelin ) and legume seeds . A unique feature of the triticin sequence is that it contains a lysine-rich repetitive domain , inserted in the hypervariable region of the typical legumin-like genes . Northern blotting of total RNA extracted from different stages of the developing wheat seed revealed that the triticin gene expression is switched on 5-10 days after anthesis ( DAA ) . There was a steady increase in the level of triticin mRNA until 20 DAA , after which it started decreasing . The maximum mRNA accumulation occurred between 17 and 20 DAA . These observations conform closely with the published data on triticin protein accumulation during grain development .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 12.00 | Title: Flavor production from edible oils and their constituents by Penicillium corylophilum .
| Journal: Shokuhin Eiseigaku Zasshi Citation: V : 43 ( 3 ) P : 160-4 Year: 2002 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12238154 Accession (PMID): 12238154 | Abstract: Production of volatile substances from edible oils and their constituents by Penicillium corylophilum was studied to clarify the mechanism of flavor production from a non-stick oil by the organism in a rice cake system .
First , edible oils from plant and animal origins were tested for flavor production .
Among the oils tested , coconut oil was the only one from which the flavor was produced .
Second , triacylglycerols consisting of fatty acids with various lengths of carbon chain ( C6 to C13 ) were studied for flavor production .
Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 .
The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols .
Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested .
Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids .
Third , fatty acids with various lengths of carbon chain ( C7 to C15 ) were studied for flavor production .
Among the fatty acids tested , flavors were produced from decanoic ( C10 ) and undecanoic ( C11 ) acids only .
The flavors also consisted of methylketones and secondary alcohols one carbon shorter than the precursor fatty acids .
Fatty acids with short carbon chains ( C7 to C9 ) completely inhibited the mould growth .
Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) .
It was also found that the non-stick oil and coconut oil contain tricaprylin and tridecanoin as triacylglycerols and decanoic acid as fatty acid .
| Matching Sentences: [ Sen. 7, subscore: 4.00 ]: Production of volatile substances from edible oils and their constituents by Penicillium corylophilum was studied to clarify the mechanism of flavor production from a non-stick oil by the organism in a rice cake system . First , edible oils from plant and animal origins were tested for flavor production . Among the oils tested , coconut oil was the only one from which the flavor was produced . Second , triacylglycerols consisting of fatty acids with various lengths of carbon chain ( C6 to C13 ) were studied for flavor production . Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 . The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols . Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested . Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids . Third , fatty acids with various lengths of carbon chain ( C7 to C15 ) were studied for flavor production . Among the fatty acids tested , flavors were produced from decanoic ( C10 ) and undecanoic ( C11 ) acids only . The flavors also consisted of methylketones and secondary alcohols one carbon shorter than the precursor fatty acids . Fatty acids with short carbon chains ( C7 to C9 ) completely inhibited the mould growth . Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) . It was also found that the non-stick oil and coconut oil contain tricaprylin and tridecanoin as triacylglycerols and decanoic acid as fatty acid . [ Sen. 14, subscore: 3.00 ]: Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 . The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols . Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested . Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids . Third , fatty acids with various lengths of carbon chain ( C7 to C15 ) were studied for flavor production . Among the fatty acids tested , flavors were produced from decanoic ( C10 ) and undecanoic ( C11 ) acids only . The flavors also consisted of methylketones and secondary alcohols one carbon shorter than the precursor fatty acids . Fatty acids with short carbon chains ( C7 to C9 ) completely inhibited the mould growth . Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) . It was also found that the non-stick oil and coconut oil contain tricaprylin and tridecanoin as triacylglycerols and decanoic acid as fatty acid . [ Sen. 4, subscore: 1.00 ]: Production of volatile substances from edible oils and their constituents by Penicillium corylophilum was studied to clarify the mechanism of flavor production from a non-stick oil by the organism in a rice cake system . First , edible oils from plant and animal origins were tested for flavor production . Among the oils tested , coconut oil was the only one from which the flavor was produced . Second , triacylglycerols consisting of fatty acids with various lengths of carbon chain ( C6 to C13 ) were studied for flavor production . Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 . The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols . Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested . Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids . Third , fatty acids with various lengths of carbon chain ( C7 to C15 ) were studied for flavor production . Among the fatty acids tested , flavors were produced from decanoic ( C10 ) and undecanoic ( C11 ) acids only . The flavors also consisted of methylketones and secondary alcohols one carbon shorter than the precursor fatty acids . Fatty acids with short carbon chains ( C7 to C9 ) completely inhibited the mould growth . Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) . [ Sen. 5, subscore: 1.00 ]: Production of volatile substances from edible oils and their constituents by Penicillium corylophilum was studied to clarify the mechanism of flavor production from a non-stick oil by the organism in a rice cake system . First , edible oils from plant and animal origins were tested for flavor production . Among the oils tested , coconut oil was the only one from which the flavor was produced . Second , triacylglycerols consisting of fatty acids with various lengths of carbon chain ( C6 to C13 ) were studied for flavor production . Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 . The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols . Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested . Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids . Third , fatty acids with various lengths of carbon chain ( C7 to C15 ) were studied for flavor production . Among the fatty acids tested , flavors were produced from decanoic ( C10 ) and undecanoic ( C11 ) acids only . The flavors also consisted of methylketones and secondary alcohols one carbon shorter than the precursor fatty acids . Fatty acids with short carbon chains ( C7 to C9 ) completely inhibited the mould growth . Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) . It was also found that the non-stick oil and coconut oil contain tricaprylin and tridecanoin as triacylglycerols and decanoic acid as fatty acid . [ Sen. 6, subscore: 1.00 ]: Production of volatile substances from edible oils and their constituents by Penicillium corylophilum was studied to clarify the mechanism of flavor production from a non-stick oil by the organism in a rice cake system . First , edible oils from plant and animal origins were tested for flavor production . Among the oils tested , coconut oil was the only one from which the flavor was produced . Second , triacylglycerols consisting of fatty acids with various lengths of carbon chain ( C6 to C13 ) were studied for flavor production . Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 . The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols . Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested . Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids . Third , fatty acids with various lengths of carbon chain ( C7 to C15 ) were studied for flavor production . Among the fatty acids tested , flavors were produced from decanoic ( C10 ) and undecanoic ( C11 ) acids only . The flavors also consisted of methylketones and secondary alcohols one carbon shorter than the precursor fatty acids . Fatty acids with short carbon chains ( C7 to C9 ) completely inhibited the mould growth . Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) . It was also found that the non-stick oil and coconut oil contain tricaprylin and tridecanoin as triacylglycerols and decanoic acid as fatty acid . [ Sen. 8, subscore: 1.00 ]: Production of volatile substances from edible oils and their constituents by Penicillium corylophilum was studied to clarify the mechanism of flavor production from a non-stick oil by the organism in a rice cake system . First , edible oils from plant and animal origins were tested for flavor production . Among the oils tested , coconut oil was the only one from which the flavor was produced . Second , triacylglycerols consisting of fatty acids with various lengths of carbon chain ( C6 to C13 ) were studied for flavor production . Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 . The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols . Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested . Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids . Third , fatty acids with various lengths of carbon chain ( C7 to C15 ) were studied for flavor production . Among the fatty acids tested , flavors were produced from decanoic ( C10 ) and undecanoic ( C11 ) acids only . The flavors also consisted of methylketones and secondary alcohols one carbon shorter than the precursor fatty acids . Fatty acids with short carbon chains ( C7 to C9 ) completely inhibited the mould growth . Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) . It was also found that the non-stick oil and coconut oil contain tricaprylin and tridecanoin as triacylglycerols and decanoic acid as fatty acid . [ Sen. 13, subscore: 1.00 ]: Second , triacylglycerols consisting of fatty acids with various lengths of carbon chain ( C6 to C13 ) were studied for flavor production . Among the triacylglycerols tested , flavors were produced from those consisting of fatty acids with carbon chains of C6 to C11 . The flavors consisted of methylketones and secondary alcohols , whose carbon chains were one carbon shorter than the precursor fatty acid molecules of the triacylglycerols . Flavors similar to that from the non-stick oil were produced from tricaprylin ( C8 ) , trinonanoin ( C9 ) , and tridecanoin ( C10 ) among the triacylglycerols tested . Formation of mould spores was more strongly suppressed by triacylglycerols with shorter chain fatty acids . Third , fatty acids with various lengths of carbon chain ( C7 to C15 ) were studied for flavor production . Among the fatty acids tested , flavors were produced from decanoic ( C10 ) and undecanoic ( C11 ) acids only . The flavors also consisted of methylketones and secondary alcohols one carbon shorter than the precursor fatty acids . Fatty acids with short carbon chains ( C7 to C9 ) completely inhibited the mould growth . Our study showed that the range of carbon chain length of fatty acids capable of the flavor production ( C10 to C11 ) was narrower than that of triacylglycerols ( C6 to C11 ) . It was also found that the non-stick oil and coconut oil contain tricaprylin and tridecanoin as triacylglycerols and decanoic acid as fatty acid .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 12.00 | Title: [ Behavior of HTO in simulated rice-water-soil ecosystem ] | Journal: Citation: V : 14 ( 2 ) P : 269-72 Year: 2003 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12827885 Accession (PMID): 12827885 | Abstract: The behavior of transportation , accumulation and disappearance of HTO ( tritium water ) in a simulated rice-water-soil ecosystem was studied by using isotope-tracer techniques for simulated pollutants , and the fitting equation was confirmed by application of the open three-compartment system model and nonlinear regression method .
The results showed that HTO in water was not only transferred to other compartments in the ecosystem , but also vaporized into atmosphere rapidly .
Both free water tritium and bound tritium were found in the rice , and tritium of hygroscopic and crystalline water was consisted in the soil .
The specific activity of free water tritium ( or tritium of hygroscopic water ) was stronger than that of bound tritium ( or tritium of crystalline water ) .
The specific activity of total tritium reduced after reaching the maximum in the rice and soil , and the bound tritium increased slowly .
The specific activity of total tritium in stem was the strongest in the rice , and reached equipoise each other in the later stage gradually .
The regression equations of accumulation and disappearance for the specific activity of total tritium in the water , soil and rice were given by analyzing the obtained data with exponential regression method .
The analysis results of variance showed that each regression equation could describe the behavior of accumulation and disappearance of HTO in the rice-water-soil ecosystems preferably . | Matching Sentences: [ Sen. 4, subscore: 4.00 ]: The behavior of transportation , accumulation and disappearance of HTO ( tritium water ) in a simulated rice-water-soil ecosystem was studied by using isotope-tracer techniques for simulated pollutants , and the fitting equation was confirmed by application of the open three-compartment system model and nonlinear regression method . The results showed that HTO in water was not only transferred to other compartments in the ecosystem , but also vaporized into atmosphere rapidly . Both free water tritium and bound tritium were found in the rice , and tritium of hygroscopic and crystalline water was consisted in the soil . The specific activity of free water tritium ( or tritium of hygroscopic water ) was stronger than that of bound tritium ( or tritium of crystalline water ) . The specific activity of total tritium reduced after reaching the maximum in the rice and soil , and the bound tritium increased slowly . The specific activity of total tritium in stem was the strongest in the rice , and reached equipoise each other in the later stage gradually . The regression equations of accumulation and disappearance for the specific activity of total tritium in the water , soil and rice were given by analyzing the obtained data with exponential regression method . The analysis results of variance showed that each regression equation could describe the behavior of accumulation and disappearance of HTO in the rice-water-soil ecosystems preferably . [ Sen. 3, subscore: 3.00 ]: The behavior of transportation , accumulation and disappearance of HTO ( tritium water ) in a simulated rice-water-soil ecosystem was studied by using isotope-tracer techniques for simulated pollutants , and the fitting equation was confirmed by application of the open three-compartment system model and nonlinear regression method . The results showed that HTO in water was not only transferred to other compartments in the ecosystem , but also vaporized into atmosphere rapidly . Both free water tritium and bound tritium were found in the rice , and tritium of hygroscopic and crystalline water was consisted in the soil . The specific activity of free water tritium ( or tritium of hygroscopic water ) was stronger than that of bound tritium ( or tritium of crystalline water ) . The specific activity of total tritium reduced after reaching the maximum in the rice and soil , and the bound tritium increased slowly . The specific activity of total tritium in stem was the strongest in the rice , and reached equipoise each other in the later stage gradually . The regression equations of accumulation and disappearance for the specific activity of total tritium in the water , soil and rice were given by analyzing the obtained data with exponential regression method . The analysis results of variance showed that each regression equation could describe the behavior of accumulation and disappearance of HTO in the rice-water-soil ecosystems preferably . [ Sen. 5, subscore: 2.00 ]: The behavior of transportation , accumulation and disappearance of HTO ( tritium water ) in a simulated rice-water-soil ecosystem was studied by using isotope-tracer techniques for simulated pollutants , and the fitting equation was confirmed by application of the open three-compartment system model and nonlinear regression method . The results showed that HTO in water was not only transferred to other compartments in the ecosystem , but also vaporized into atmosphere rapidly . Both free water tritium and bound tritium were found in the rice , and tritium of hygroscopic and crystalline water was consisted in the soil . The specific activity of free water tritium ( or tritium of hygroscopic water ) was stronger than that of bound tritium ( or tritium of crystalline water ) . The specific activity of total tritium reduced after reaching the maximum in the rice and soil , and the bound tritium increased slowly . The specific activity of total tritium in stem was the strongest in the rice , and reached equipoise each other in the later stage gradually . The regression equations of accumulation and disappearance for the specific activity of total tritium in the water , soil and rice were given by analyzing the obtained data with exponential regression method . The analysis results of variance showed that each regression equation could describe the behavior of accumulation and disappearance of HTO in the rice-water-soil ecosystems preferably . [ Sen. 1, subscore: 1.00 ]: The behavior of transportation , accumulation and disappearance of HTO ( tritium water ) in a simulated rice-water-soil ecosystem was studied by using isotope-tracer techniques for simulated pollutants , and the fitting equation was confirmed by application of the open three-compartment system model and nonlinear regression method . The results showed that HTO in water was not only transferred to other compartments in the ecosystem , but also vaporized into atmosphere rapidly . Both free water tritium and bound tritium were found in the rice , and tritium of hygroscopic and crystalline water was consisted in the soil . The specific activity of free water tritium ( or tritium of hygroscopic water ) was stronger than that of bound tritium ( or tritium of crystalline water ) . The specific activity of total tritium reduced after reaching the maximum in the rice and soil , and the bound tritium increased slowly . The specific activity of total tritium in stem was the strongest in the rice , and reached equipoise each other in the later stage gradually . The regression equations of accumulation and disappearance for the specific activity of total tritium in the water , soil and rice were given by analyzing the obtained data with exponential regression method . The analysis results of variance showed that each regression equation could describe the behavior of accumulation and disappearance of HTO in the rice-water-soil ecosystems preferably . [ Sen. 6, subscore: 1.00 ]: The behavior of transportation , accumulation and disappearance of HTO ( tritium water ) in a simulated rice-water-soil ecosystem was studied by using isotope-tracer techniques for simulated pollutants , and the fitting equation was confirmed by application of the open three-compartment system model and nonlinear regression method . The results showed that HTO in water was not only transferred to other compartments in the ecosystem , but also vaporized into atmosphere rapidly . Both free water tritium and bound tritium were found in the rice , and tritium of hygroscopic and crystalline water was consisted in the soil . The specific activity of free water tritium ( or tritium of hygroscopic water ) was stronger than that of bound tritium ( or tritium of crystalline water ) . The specific activity of total tritium reduced after reaching the maximum in the rice and soil , and the bound tritium increased slowly . The specific activity of total tritium in stem was the strongest in the rice , and reached equipoise each other in the later stage gradually . The regression equations of accumulation and disappearance for the specific activity of total tritium in the water , soil and rice were given by analyzing the obtained data with exponential regression method . The analysis results of variance showed that each regression equation could describe the behavior of accumulation and disappearance of HTO in the rice-water-soil ecosystems preferably . [ Sen. 7, subscore: 1.00 ]: The behavior of transportation , accumulation and disappearance of HTO ( tritium water ) in a simulated rice-water-soil ecosystem was studied by using isotope-tracer techniques for simulated pollutants , and the fitting equation was confirmed by application of the open three-compartment system model and nonlinear regression method . The results showed that HTO in water was not only transferred to other compartments in the ecosystem , but also vaporized into atmosphere rapidly . Both free water tritium and bound tritium were found in the rice , and tritium of hygroscopic and crystalline water was consisted in the soil . The specific activity of free water tritium ( or tritium of hygroscopic water ) was stronger than that of bound tritium ( or tritium of crystalline water ) . The specific activity of total tritium reduced after reaching the maximum in the rice and soil , and the bound tritium increased slowly . The specific activity of total tritium in stem was the strongest in the rice , and reached equipoise each other in the later stage gradually . The regression equations of accumulation and disappearance for the specific activity of total tritium in the water , soil and rice were given by analyzing the obtained data with exponential regression method . The analysis results of variance showed that each regression equation could describe the behavior of accumulation and disappearance of HTO in the rice-water-soil ecosystems preferably .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 12.00 | Title: Allelochemical tricin in rice hull and its aurone isomer against rice seedling rot disease .
| Journal: Pest Manag Sci Citation: V : 66 P : 1018-24 Year: 2010 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub20730995 Accession (PMID): 20730995 | Abstract: BACKGROUND : One promising area of rice disease management is the potential of exploiting biological control agents .
Rice seedling rot disease caused by soil-borne pathogenic fungi has become a dominant disease problem because of greater use of direct seeding .
Rice hull has been potentially used to control paddy weeds , but little information is available on rice disease .
This study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull , and to assess fungicidal activity of tricin and its synthesised aurone isomer , with an attempt to develop an allelochemical-based fungicide against rice seedling rot disease .
RESULTS : Tricin was detected in all hulls of 12 rice cultivars tested , but its contents in rice hulls varied greatly with the cultivar and genotype .
Tricin in rice hulls was released into the soil once amended .
Disease incidence was significantly reduced by soil amended with rice hull .
Tricin-rich rice hull amendment greatly suppressed soil-borne pathogenic fungi Fusarium oxysporum Schlecht . and Rhizoctonia solani Kuhn which cause rice seedling rot disease .
In attempting to obtain enough tricin for further experiments , the aurone isomer ( 5 , 7 , 4-trihydroxy-3 , 5-dimethoxyaurone ) of tricin rather than tricin itself was unexpectedly synthesised .
This aurone isomer had much stronger fungicidal activity on both F oxysporum and R solani than tricin itself .
CONCLUSION : Soil amended with tricin-rich rice hull was associated with reduced risk of developing seedling rot disease .
The tricin isomer , aurone , is more effective against rice seedling rot disease than tricin itself , making it an ideal lead compound for new fungicide discovery .
| Matching Sentences: [ Sen. 9, subscore: 3.00 ]: BACKGROUND : One promising area of rice disease management is the potential of exploiting biological control agents . Rice seedling rot disease caused by soil-borne pathogenic fungi has become a dominant disease problem because of greater use of direct seeding . Rice hull has been potentially used to control paddy weeds , but little information is available on rice disease . This study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull , and to assess fungicidal activity of tricin and its synthesised aurone isomer , with an attempt to develop an allelochemical-based fungicide against rice seedling rot disease . RESULTS : Tricin was detected in all hulls of 12 rice cultivars tested , but its contents in rice hulls varied greatly with the cultivar and genotype . Tricin in rice hulls was released into the soil once amended . Disease incidence was significantly reduced by soil amended with rice hull . Tricin-rich rice hull amendment greatly suppressed soil-borne pathogenic fungi Fusarium oxysporum Schlecht . and Rhizoctonia solani Kuhn which cause rice seedling rot disease . In attempting to obtain enough tricin for further experiments , the aurone isomer ( 5 , 7 , 4-trihydroxy-3 , 5-dimethoxyaurone ) of tricin rather than tricin itself was unexpectedly synthesised . This aurone isomer had much stronger fungicidal activity on both F oxysporum and R solani than tricin itself . CONCLUSION : Soil amended with tricin-rich rice hull was associated with reduced risk of developing seedling rot disease . The tricin isomer , aurone , is more effective against rice seedling rot disease than tricin itself , making it an ideal lead compound for new fungicide discovery . [ Sen. 4, subscore: 2.00 ]: BACKGROUND : One promising area of rice disease management is the potential of exploiting biological control agents . Rice seedling rot disease caused by soil-borne pathogenic fungi has become a dominant disease problem because of greater use of direct seeding . Rice hull has been potentially used to control paddy weeds , but little information is available on rice disease . This study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull , and to assess fungicidal activity of tricin and its synthesised aurone isomer , with an attempt to develop an allelochemical-based fungicide against rice seedling rot disease . RESULTS : Tricin was detected in all hulls of 12 rice cultivars tested , but its contents in rice hulls varied greatly with the cultivar and genotype . Tricin in rice hulls was released into the soil once amended . Disease incidence was significantly reduced by soil amended with rice hull . Tricin-rich rice hull amendment greatly suppressed soil-borne pathogenic fungi Fusarium oxysporum Schlecht . and Rhizoctonia solani Kuhn which cause rice seedling rot disease . In attempting to obtain enough tricin for further experiments , the aurone isomer ( 5 , 7 , 4-trihydroxy-3 , 5-dimethoxyaurone ) of tricin rather than tricin itself was unexpectedly synthesised . This aurone isomer had much stronger fungicidal activity on both F oxysporum and R solani than tricin itself . CONCLUSION : Soil amended with tricin-rich rice hull was associated with reduced risk of developing seedling rot disease . The tricin isomer , aurone , is more effective against rice seedling rot disease than tricin itself , making it an ideal lead compound for new fungicide discovery . [ Sen. 12, subscore: 2.00 ]: Rice hull has been potentially used to control paddy weeds , but little information is available on rice disease . This study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull , and to assess fungicidal activity of tricin and its synthesised aurone isomer , with an attempt to develop an allelochemical-based fungicide against rice seedling rot disease . RESULTS : Tricin was detected in all hulls of 12 rice cultivars tested , but its contents in rice hulls varied greatly with the cultivar and genotype . Tricin in rice hulls was released into the soil once amended . Disease incidence was significantly reduced by soil amended with rice hull . Tricin-rich rice hull amendment greatly suppressed soil-borne pathogenic fungi Fusarium oxysporum Schlecht . and Rhizoctonia solani Kuhn which cause rice seedling rot disease . In attempting to obtain enough tricin for further experiments , the aurone isomer ( 5 , 7 , 4-trihydroxy-3 , 5-dimethoxyaurone ) of tricin rather than tricin itself was unexpectedly synthesised . This aurone isomer had much stronger fungicidal activity on both F oxysporum and R solani than tricin itself . CONCLUSION : Soil amended with tricin-rich rice hull was associated with reduced risk of developing seedling rot disease . The tricin isomer , aurone , is more effective against rice seedling rot disease than tricin itself , making it an ideal lead compound for new fungicide discovery . [ Sen. 5, subscore: 1.00 ]: BACKGROUND : One promising area of rice disease management is the potential of exploiting biological control agents . Rice seedling rot disease caused by soil-borne pathogenic fungi has become a dominant disease problem because of greater use of direct seeding . Rice hull has been potentially used to control paddy weeds , but little information is available on rice disease . This study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull , and to assess fungicidal activity of tricin and its synthesised aurone isomer , with an attempt to develop an allelochemical-based fungicide against rice seedling rot disease . RESULTS : Tricin was detected in all hulls of 12 rice cultivars tested , but its contents in rice hulls varied greatly with the cultivar and genotype . Tricin in rice hulls was released into the soil once amended . Disease incidence was significantly reduced by soil amended with rice hull . Tricin-rich rice hull amendment greatly suppressed soil-borne pathogenic fungi Fusarium oxysporum Schlecht . and Rhizoctonia solani Kuhn which cause rice seedling rot disease . In attempting to obtain enough tricin for further experiments , the aurone isomer ( 5 , 7 , 4-trihydroxy-3 , 5-dimethoxyaurone ) of tricin rather than tricin itself was unexpectedly synthesised . This aurone isomer had much stronger fungicidal activity on both F oxysporum and R solani than tricin itself . CONCLUSION : Soil amended with tricin-rich rice hull was associated with reduced risk of developing seedling rot disease . The tricin isomer , aurone , is more effective against rice seedling rot disease than tricin itself , making it an ideal lead compound for new fungicide discovery . [ Sen. 6, subscore: 1.00 ]: BACKGROUND : One promising area of rice disease management is the potential of exploiting biological control agents . Rice seedling rot disease caused by soil-borne pathogenic fungi has become a dominant disease problem because of greater use of direct seeding . Rice hull has been potentially used to control paddy weeds , but little information is available on rice disease . This study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull , and to assess fungicidal activity of tricin and its synthesised aurone isomer , with an attempt to develop an allelochemical-based fungicide against rice seedling rot disease . RESULTS : Tricin was detected in all hulls of 12 rice cultivars tested , but its contents in rice hulls varied greatly with the cultivar and genotype . Tricin in rice hulls was released into the soil once amended . Disease incidence was significantly reduced by soil amended with rice hull . Tricin-rich rice hull amendment greatly suppressed soil-borne pathogenic fungi Fusarium oxysporum Schlecht . and Rhizoctonia solani Kuhn which cause rice seedling rot disease . In attempting to obtain enough tricin for further experiments , the aurone isomer ( 5 , 7 , 4-trihydroxy-3 , 5-dimethoxyaurone ) of tricin rather than tricin itself was unexpectedly synthesised . This aurone isomer had much stronger fungicidal activity on both F oxysporum and R solani than tricin itself . CONCLUSION : Soil amended with tricin-rich rice hull was associated with reduced risk of developing seedling rot disease . The tricin isomer , aurone , is more effective against rice seedling rot disease than tricin itself , making it an ideal lead compound for new fungicide discovery . [ Sen. 8, subscore: 1.00 ]: BACKGROUND : One promising area of rice disease management is the potential of exploiting biological control agents . Rice seedling rot disease caused by soil-borne pathogenic fungi has become a dominant disease problem because of greater use of direct seeding . Rice hull has been potentially used to control paddy weeds , but little information is available on rice disease . This study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull , and to assess fungicidal activity of tricin and its synthesised aurone isomer , with an attempt to develop an allelochemical-based fungicide against rice seedling rot disease . RESULTS : Tricin was detected in all hulls of 12 rice cultivars tested , but its contents in rice hulls varied greatly with the cultivar and genotype . Tricin in rice hulls was released into the soil once amended . Disease incidence was significantly reduced by soil amended with rice hull . Tricin-rich rice hull amendment greatly suppressed soil-borne pathogenic fungi Fusarium oxysporum Schlecht . and Rhizoctonia solani Kuhn which cause rice seedling rot disease . In attempting to obtain enough tricin for further experiments , the aurone isomer ( 5 , 7 , 4-trihydroxy-3 , 5-dimethoxyaurone ) of tricin rather than tricin itself was unexpectedly synthesised . This aurone isomer had much stronger fungicidal activity on both F oxysporum and R solani than tricin itself . CONCLUSION : Soil amended with tricin-rich rice hull was associated with reduced risk of developing seedling rot disease . The tricin isomer , aurone , is more effective against rice seedling rot disease than tricin itself , making it an ideal lead compound for new fungicide discovery . [ Sen. 10, subscore: 1.00 ]: BACKGROUND : One promising area of rice disease management is the potential of exploiting biological control agents . Rice seedling rot disease caused by soil-borne pathogenic fungi has become a dominant disease problem because of greater use of direct seeding . Rice hull has been potentially used to control paddy weeds , but little information is available on rice disease . This study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull , and to assess fungicidal activity of tricin and its synthesised aurone isomer , with an attempt to develop an allelochemical-based fungicide against rice seedling rot disease . RESULTS : Tricin was detected in all hulls of 12 rice cultivars tested , but its contents in rice hulls varied greatly with the cultivar and genotype . Tricin in rice hulls was released into the soil once amended . Disease incidence was significantly reduced by soil amended with rice hull . Tricin-rich rice hull amendment greatly suppressed soil-borne pathogenic fungi Fusarium oxysporum Schlecht . and Rhizoctonia solani Kuhn which cause rice seedling rot disease . In attempting to obtain enough tricin for further experiments , the aurone isomer ( 5 , 7 , 4-trihydroxy-3 , 5-dimethoxyaurone ) of tricin rather than tricin itself was unexpectedly synthesised . This aurone isomer had much stronger fungicidal activity on both F oxysporum and R solani than tricin itself . CONCLUSION : Soil amended with tricin-rich rice hull was associated with reduced risk of developing seedling rot disease . The tricin isomer , aurone , is more effective against rice seedling rot disease than tricin itself , making it an ideal lead compound for new fungicide discovery . [ Sen. 11, subscore: 1.00 ]: Rice seedling rot disease caused by soil-borne pathogenic fungi has become a dominant disease problem because of greater use of direct seeding . Rice hull has been potentially used to control paddy weeds , but little information is available on rice disease . This study was conducted to investigate the relationships between disease incidence and soil amended with tricin-releasing rice hull , and to assess fungicidal activity of tricin and its synthesised aurone isomer , with an attempt to develop an allelochemical-based fungicide against rice seedling rot disease . RESULTS : Tricin was detected in all hulls of 12 rice cultivars tested , but its contents in rice hulls varied greatly with the cultivar and genotype . Tricin in rice hulls was released into the soil once amended . Disease incidence was significantly reduced by soil amended with rice hull . Tricin-rich rice hull amendment greatly suppressed soil-borne pathogenic fungi Fusarium oxysporum Schlecht . and Rhizoctonia solani Kuhn which cause rice seedling rot disease . In attempting to obtain enough tricin for further experiments , the aurone isomer ( 5 , 7 , 4-trihydroxy-3 , 5-dimethoxyaurone ) of tricin rather than tricin itself was unexpectedly synthesised . This aurone isomer had much stronger fungicidal activity on both F oxysporum and R solani than tricin itself . CONCLUSION : Soil amended with tricin-rich rice hull was associated with reduced risk of developing seedling rot disease . The tricin isomer , aurone , is more effective against rice seedling rot disease than tricin itself , making it an ideal lead compound for new fungicide discovery .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 | Title: Use of a nonionic detergent ( Triton WR 1339 ) in healthy cats to assess hepatic secretion of triglyceride .
| Journal: Am .
J Vet . Res . Citation: V : 61 ( 8 ) P : 941-50 Year: 2000 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10951988 Accession (PMID): 10951988 | Abstract: OBJECTIVE : To determine whether a nonionic detergent ( Triton WR 1339 ) can be used in cats to assess hepatic secretion of triglyceride .
ANIMALS : 28 healthy cats .
PROCEDURE : Triton WR 1339 was administered IV according to the following schedule : 5 , 50 , 150 , and 250 mg/kg of body weight .
Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group .
Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies .
RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia .
Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours .
Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups .
At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg .
The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later .
Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response .
CONCLUSIONS AND CLINICAL RELEVANCE : Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion , although some cats may have increased RBC osmotic fragility .
Higher dosages caused substantial adverse effects , whereas lower dosages did not alter plasma triglyceride concentration . | Matching Sentences: [ Sen. 1, subscore: 2.00 ]: OBJECTIVE : To determine whether a nonionic detergent ( Triton WR 1339 ) can be used in cats to assess hepatic secretion of triglyceride . ANIMALS : 28 healthy cats . PROCEDURE : Triton WR 1339 was administered IV according to the following schedule : 5 , 50 , 150 , and 250 mg/kg of body weight . Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group . Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies . RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia . Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours . Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups . At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg . The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later . [ Sen. 12, subscore: 2.00 ]: PROCEDURE : Triton WR 1339 was administered IV according to the following schedule : 5 , 50 , 150 , and 250 mg/kg of body weight . Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group . Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies . RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia . Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours . Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups . At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg . The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later . Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response . CONCLUSIONS AND CLINICAL RELEVANCE : Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion , although some cats may have increased RBC osmotic fragility . Higher dosages caused substantial adverse effects , whereas lower dosages did not alter plasma triglyceride concentration . [ Sen. 3, subscore: 1.00 ]: OBJECTIVE : To determine whether a nonionic detergent ( Triton WR 1339 ) can be used in cats to assess hepatic secretion of triglyceride . ANIMALS : 28 healthy cats . PROCEDURE : Triton WR 1339 was administered IV according to the following schedule : 5 , 50 , 150 , and 250 mg/kg of body weight . Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group . Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies . RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia . Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours . Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups . At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg . The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later . Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response . CONCLUSIONS AND CLINICAL RELEVANCE : Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion , although some cats may have increased RBC osmotic fragility . [ Sen. 5, subscore: 1.00 ]: OBJECTIVE : To determine whether a nonionic detergent ( Triton WR 1339 ) can be used in cats to assess hepatic secretion of triglyceride . ANIMALS : 28 healthy cats . PROCEDURE : Triton WR 1339 was administered IV according to the following schedule : 5 , 50 , 150 , and 250 mg/kg of body weight . Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group . Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies . RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia . Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours . Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups . At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg . The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later . Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response . CONCLUSIONS AND CLINICAL RELEVANCE : Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion , although some cats may have increased RBC osmotic fragility . Higher dosages caused substantial adverse effects , whereas lower dosages did not alter plasma triglyceride concentration . [ Sen. 6, subscore: 1.00 ]: OBJECTIVE : To determine whether a nonionic detergent ( Triton WR 1339 ) can be used in cats to assess hepatic secretion of triglyceride . ANIMALS : 28 healthy cats . PROCEDURE : Triton WR 1339 was administered IV according to the following schedule : 5 , 50 , 150 , and 250 mg/kg of body weight . Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group . Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies . RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia . Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours . Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups . At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg . The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later . Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response . CONCLUSIONS AND CLINICAL RELEVANCE : Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion , although some cats may have increased RBC osmotic fragility . Higher dosages caused substantial adverse effects , whereas lower dosages did not alter plasma triglyceride concentration . [ Sen. 7, subscore: 1.00 ]: OBJECTIVE : To determine whether a nonionic detergent ( Triton WR 1339 ) can be used in cats to assess hepatic secretion of triglyceride . ANIMALS : 28 healthy cats . PROCEDURE : Triton WR 1339 was administered IV according to the following schedule : 5 , 50 , 150 , and 250 mg/kg of body weight . Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group . Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies . RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia . Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours . Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups . At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg . The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later . Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response . CONCLUSIONS AND CLINICAL RELEVANCE : Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion , although some cats may have increased RBC osmotic fragility . Higher dosages caused substantial adverse effects , whereas lower dosages did not alter plasma triglyceride concentration . [ Sen. 8, subscore: 1.00 ]: OBJECTIVE : To determine whether a nonionic detergent ( Triton WR 1339 ) can be used in cats to assess hepatic secretion of triglyceride . ANIMALS : 28 healthy cats . PROCEDURE : Triton WR 1339 was administered IV according to the following schedule : 5 , 50 , 150 , and 250 mg/kg of body weight . Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group . Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies . RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia . Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours . Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups . At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg . The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later . Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response . CONCLUSIONS AND CLINICAL RELEVANCE : Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion , although some cats may have increased RBC osmotic fragility . Higher dosages caused substantial adverse effects , whereas lower dosages did not alter plasma triglyceride concentration . [ Sen. 11, subscore: 1.00 ]: ANIMALS : 28 healthy cats . PROCEDURE : Triton WR 1339 was administered IV according to the following schedule : 5 , 50 , 150 , and 250 mg/kg of body weight . Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group . Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies . RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia . Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours . Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups . At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg . The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later . Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response . CONCLUSIONS AND CLINICAL RELEVANCE : Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion , although some cats may have increased RBC osmotic fragility . Higher dosages caused substantial adverse effects , whereas lower dosages did not alter plasma triglyceride concentration . [ Sen. 13, subscore: 1.00 ]: Control cats did not receive an injection or received 0 . 9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group . Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies . RESULTS : Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia . Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours . Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups . At 12 and 24 hours , cholesterol was significantly higher in cats receiving 250 mg/kg . The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg ; 1 of these cats developed severe icterus and died 5 days later . Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response . CONCLUSIONS AND CLINICAL RELEVANCE : Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion , although some cats may have increased RBC osmotic fragility . Higher dosages caused substantial adverse effects , whereas lower dosages did not alter plasma triglyceride concentration .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 | Title: Primary Trisomics of Rice : Origin , Morphology , Cytology and Use in Linkage Mapping .
| Journal: Citation: V : 107 ( 1 ) P : 141-163 Year: 1984 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub17246212 Accession (PMID): 17246212 | Abstract: Twelve primary trisomics of Oryza sativa L were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of IR36 , a widely grown high yielding rice variety .
Eleven trisomics can be identified morphologically from one another and from diploids .
However , triplo 11 is difficult to distinguish from diploid sibs . -The extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis , and the chromosomes were numbered according to their length at this stage .
The major distinguishing features of each pachytene chromosome were redescribed . -The female transmission rates varied from 15 . 5% for triplo 1 , the longest chromosome , to 43 . 9% for triplo 12 , the shortest chromosome .
Seven of the 12 primary trisomics transmitted the extra chromosome through the male .
The low level of chromosomal imbalance tolerated by rice and other evidence are interpreted to indicate that this species is a basic diploid . -Genetic segregation for 22 marker genes in the trisomic progenies was studied .
Of a possible 264 combinations , involving 22 genes and 12 trisomics , 120 were examined .
Marker genes for each of the 12 chromosomes were identified .
The results helped establish associations between linkage groups and cytologically identifiable chromosomes of rice for the first time .
Relationships between various systems of numbering chromosomes , trisomics , linkage groups and marker genes are described , and a revised linkage map of rice is presented . | Matching Sentences: [ Sen. 1, subscore: 2.00 ]: Twelve primary trisomics of Oryza sativa L were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of IR36 , a widely grown high yielding rice variety . Eleven trisomics can be identified morphologically from one another and from diploids . However , triplo 11 is difficult to distinguish from diploid sibs . -The extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis , and the chromosomes were numbered according to their length at this stage . The major distinguishing features of each pachytene chromosome were redescribed . -The female transmission rates varied from 15 . 5% for triplo 1 , the longest chromosome , to 43 . 9% for triplo 12 , the shortest chromosome . Seven of the 12 primary trisomics transmitted the extra chromosome through the male . The low level of chromosomal imbalance tolerated by rice and other evidence are interpreted to indicate that this species is a basic diploid . -Genetic segregation for 22 marker genes in the trisomic progenies was studied . Of a possible 264 combinations , involving 22 genes and 12 trisomics , 120 were examined . Marker genes for each of the 12 chromosomes were identified . The results helped establish associations between linkage groups and cytologically identifiable chromosomes of rice for the first time . Relationships between various systems of numbering chromosomes , trisomics , linkage groups and marker genes are described , and a revised linkage map of rice is presented . [ Sen. 3, subscore: 2.00 ]: Twelve primary trisomics of Oryza sativa L were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of IR36 , a widely grown high yielding rice variety . Eleven trisomics can be identified morphologically from one another and from diploids . However , triplo 11 is difficult to distinguish from diploid sibs . -The extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis , and the chromosomes were numbered according to their length at this stage . The major distinguishing features of each pachytene chromosome were redescribed . -The female transmission rates varied from 15 . 5% for triplo 1 , the longest chromosome , to 43 . 9% for triplo 12 , the shortest chromosome . Seven of the 12 primary trisomics transmitted the extra chromosome through the male . The low level of chromosomal imbalance tolerated by rice and other evidence are interpreted to indicate that this species is a basic diploid . -Genetic segregation for 22 marker genes in the trisomic progenies was studied . Of a possible 264 combinations , involving 22 genes and 12 trisomics , 120 were examined . Marker genes for each of the 12 chromosomes were identified . The results helped establish associations between linkage groups and cytologically identifiable chromosomes of rice for the first time . Relationships between various systems of numbering chromosomes , trisomics , linkage groups and marker genes are described , and a revised linkage map of rice is presented . [ Sen. 4, subscore: 2.00 ]: Twelve primary trisomics of Oryza sativa L were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of IR36 , a widely grown high yielding rice variety . Eleven trisomics can be identified morphologically from one another and from diploids . However , triplo 11 is difficult to distinguish from diploid sibs . -The extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis , and the chromosomes were numbered according to their length at this stage . The major distinguishing features of each pachytene chromosome were redescribed . -The female transmission rates varied from 15 . 5% for triplo 1 , the longest chromosome , to 43 . 9% for triplo 12 , the shortest chromosome . Seven of the 12 primary trisomics transmitted the extra chromosome through the male . The low level of chromosomal imbalance tolerated by rice and other evidence are interpreted to indicate that this species is a basic diploid . -Genetic segregation for 22 marker genes in the trisomic progenies was studied . Of a possible 264 combinations , involving 22 genes and 12 trisomics , 120 were examined . Marker genes for each of the 12 chromosomes were identified . The results helped establish associations between linkage groups and cytologically identifiable chromosomes of rice for the first time . Relationships between various systems of numbering chromosomes , trisomics , linkage groups and marker genes are described , and a revised linkage map of rice is presented . [ Sen. 2, subscore: 1.00 ]: Twelve primary trisomics of Oryza sativa L were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of IR36 , a widely grown high yielding rice variety . Eleven trisomics can be identified morphologically from one another and from diploids . However , triplo 11 is difficult to distinguish from diploid sibs . -The extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis , and the chromosomes were numbered according to their length at this stage . The major distinguishing features of each pachytene chromosome were redescribed . -The female transmission rates varied from 15 . 5% for triplo 1 , the longest chromosome , to 43 . 9% for triplo 12 , the shortest chromosome . Seven of the 12 primary trisomics transmitted the extra chromosome through the male . The low level of chromosomal imbalance tolerated by rice and other evidence are interpreted to indicate that this species is a basic diploid . -Genetic segregation for 22 marker genes in the trisomic progenies was studied . Of a possible 264 combinations , involving 22 genes and 12 trisomics , 120 were examined . Marker genes for each of the 12 chromosomes were identified . The results helped establish associations between linkage groups and cytologically identifiable chromosomes of rice for the first time . Relationships between various systems of numbering chromosomes , trisomics , linkage groups and marker genes are described , and a revised linkage map of rice is presented . [ Sen. 5, subscore: 1.00 ]: Twelve primary trisomics of Oryza sativa L were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of IR36 , a widely grown high yielding rice variety . Eleven trisomics can be identified morphologically from one another and from diploids . However , triplo 11 is difficult to distinguish from diploid sibs . -The extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis , and the chromosomes were numbered according to their length at this stage . The major distinguishing features of each pachytene chromosome were redescribed . -The female transmission rates varied from 15 . 5% for triplo 1 , the longest chromosome , to 43 . 9% for triplo 12 , the shortest chromosome . Seven of the 12 primary trisomics transmitted the extra chromosome through the male . The low level of chromosomal imbalance tolerated by rice and other evidence are interpreted to indicate that this species is a basic diploid . -Genetic segregation for 22 marker genes in the trisomic progenies was studied . Of a possible 264 combinations , involving 22 genes and 12 trisomics , 120 were examined . Marker genes for each of the 12 chromosomes were identified . The results helped establish associations between linkage groups and cytologically identifiable chromosomes of rice for the first time . Relationships between various systems of numbering chromosomes , trisomics , linkage groups and marker genes are described , and a revised linkage map of rice is presented . [ Sen. 6, subscore: 1.00 ]: Twelve primary trisomics of Oryza sativa L were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of IR36 , a widely grown high yielding rice variety . Eleven trisomics can be identified morphologically from one another and from diploids . However , triplo 11 is difficult to distinguish from diploid sibs . -The extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis , and the chromosomes were numbered according to their length at this stage . The major distinguishing features of each pachytene chromosome were redescribed . -The female transmission rates varied from 15 . 5% for triplo 1 , the longest chromosome , to 43 . 9% for triplo 12 , the shortest chromosome . Seven of the 12 primary trisomics transmitted the extra chromosome through the male . The low level of chromosomal imbalance tolerated by rice and other evidence are interpreted to indicate that this species is a basic diploid . -Genetic segregation for 22 marker genes in the trisomic progenies was studied . Of a possible 264 combinations , involving 22 genes and 12 trisomics , 120 were examined . Marker genes for each of the 12 chromosomes were identified . The results helped establish associations between linkage groups and cytologically identifiable chromosomes of rice for the first time . Relationships between various systems of numbering chromosomes , trisomics , linkage groups and marker genes are described , and a revised linkage map of rice is presented . [ Sen. 7, subscore: 1.00 ]: Twelve primary trisomics of Oryza sativa L were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of IR36 , a widely grown high yielding rice variety . Eleven trisomics can be identified morphologically from one another and from diploids . However , triplo 11 is difficult to distinguish from diploid sibs . -The extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis , and the chromosomes were numbered according to their length at this stage . The major distinguishing features of each pachytene chromosome were redescribed . -The female transmission rates varied from 15 . 5% for triplo 1 , the longest chromosome , to 43 . 9% for triplo 12 , the shortest chromosome . Seven of the 12 primary trisomics transmitted the extra chromosome through the male . The low level of chromosomal imbalance tolerated by rice and other evidence are interpreted to indicate that this species is a basic diploid . -Genetic segregation for 22 marker genes in the trisomic progenies was studied . Of a possible 264 combinations , involving 22 genes and 12 trisomics , 120 were examined . Marker genes for each of the 12 chromosomes were identified . The results helped establish associations between linkage groups and cytologically identifiable chromosomes of rice for the first time . Relationships between various systems of numbering chromosomes , trisomics , linkage groups and marker genes are described , and a revised linkage map of rice is presented . [ Sen. 10, subscore: 1.00 ]: Twelve primary trisomics of Oryza sativa L were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of IR36 , a widely grown high yielding rice variety . Eleven trisomics can be identified morphologically from one another and from diploids . However , triplo 11 is difficult to distinguish from diploid sibs . -The extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis , and the chromosomes were numbered according to their length at this stage . The major distinguishing features of each pachytene chromosome were redescribed . -The female transmission rates varied from 15 . 5% for triplo 1 , the longest chromosome , to 43 . 9% for triplo 12 , the shortest chromosome . Seven of the 12 primary trisomics transmitted the extra chromosome through the male . The low level of chromosomal imbalance tolerated by rice and other evidence are interpreted to indicate that this species is a basic diploid . -Genetic segregation for 22 marker genes in the trisomic progenies was studied . Of a possible 264 combinations , involving 22 genes and 12 trisomics , 120 were examined . Marker genes for each of the 12 chromosomes were identified . The results helped establish associations between linkage groups and cytologically identifiable chromosomes of rice for the first time . Relationships between various systems of numbering chromosomes , trisomics , linkage groups and marker genes are described , and a revised linkage map of rice is presented .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 | Title: Genetically engineered Fusarium as a tool to evaluate the effects of environmental factors on initiation of trichothecene biosynthesis .
| Journal: FEMS Microbiol Lett Citation: V : 275 P : 53-61 Year: 2007 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub17711459 Accession (PMID): 17711459 | Abstract: Fusarium graminearum was engineered for expression of enhanced green fluorescent protein gene ( egfp ) as a reporter regulated in a manner similar to Tri5 , a key pathway gene in trichothecene biosynthesis .
Using the transgenic fungus , it was found that the reporter gene was induced to express in aerial hyphae developed on trichothecene noninducing medium YG solidified by agar .
Unexpectedly , the transcriptional activation of egfp was markedly suppressed by adding NaCl that does not significantly affect fungal growth .
As suggested by these findings , wild-type F graminearum that formed aerial hyphae on YG agar plates produced trichothecenes and the production was effectively suppressed by adding 1% NaCl to the agar .
To evaluate the effects of abiotic stress on the expression of trichothecene biosynthesis ( Tri ) genes , a sensitive plate assay was established using GYEP medium ( which very weakly induces trichothecene production ) solidified with gellan gum .
Using this assay , triazole fungicides were shown to cause transcriptional activation of egfp at sublethal concentrations .
Indeed , trichothecene production significantly increased when F graminearum was grown in rice medium ( which moderately induces trichothecene ) amended with low doses of tebuconazole .
The real-time monitoring system described here may help predict the risks of trichothecene contamination by the fungus under various environmental conditions .
| Matching Sentences: [ Sen. 5, subscore: 3.00 ]: Fusarium graminearum was engineered for expression of enhanced green fluorescent protein gene ( egfp ) as a reporter regulated in a manner similar to Tri5 , a key pathway gene in trichothecene biosynthesis . Using the transgenic fungus , it was found that the reporter gene was induced to express in aerial hyphae developed on trichothecene noninducing medium YG solidified by agar . Unexpectedly , the transcriptional activation of egfp was markedly suppressed by adding NaCl that does not significantly affect fungal growth . As suggested by these findings , wild-type F graminearum that formed aerial hyphae on YG agar plates produced trichothecenes and the production was effectively suppressed by adding 1% NaCl to the agar . To evaluate the effects of abiotic stress on the expression of trichothecene biosynthesis ( Tri ) genes , a sensitive plate assay was established using GYEP medium ( which very weakly induces trichothecene production ) solidified with gellan gum . Using this assay , triazole fungicides were shown to cause transcriptional activation of egfp at sublethal concentrations . Indeed , trichothecene production significantly increased when F graminearum was grown in rice medium ( which moderately induces trichothecene ) amended with low doses of tebuconazole . The real-time monitoring system described here may help predict the risks of trichothecene contamination by the fungus under various environmental conditions . [ Sen. 1, subscore: 2.00 ]: Fusarium graminearum was engineered for expression of enhanced green fluorescent protein gene ( egfp ) as a reporter regulated in a manner similar to Tri5 , a key pathway gene in trichothecene biosynthesis . Using the transgenic fungus , it was found that the reporter gene was induced to express in aerial hyphae developed on trichothecene noninducing medium YG solidified by agar . Unexpectedly , the transcriptional activation of egfp was markedly suppressed by adding NaCl that does not significantly affect fungal growth . As suggested by these findings , wild-type F graminearum that formed aerial hyphae on YG agar plates produced trichothecenes and the production was effectively suppressed by adding 1% NaCl to the agar . To evaluate the effects of abiotic stress on the expression of trichothecene biosynthesis ( Tri ) genes , a sensitive plate assay was established using GYEP medium ( which very weakly induces trichothecene production ) solidified with gellan gum . Using this assay , triazole fungicides were shown to cause transcriptional activation of egfp at sublethal concentrations . Indeed , trichothecene production significantly increased when F graminearum was grown in rice medium ( which moderately induces trichothecene ) amended with low doses of tebuconazole . The real-time monitoring system described here may help predict the risks of trichothecene contamination by the fungus under various environmental conditions . [ Sen. 7, subscore: 2.00 ]: Fusarium graminearum was engineered for expression of enhanced green fluorescent protein gene ( egfp ) as a reporter regulated in a manner similar to Tri5 , a key pathway gene in trichothecene biosynthesis . Using the transgenic fungus , it was found that the reporter gene was induced to express in aerial hyphae developed on trichothecene noninducing medium YG solidified by agar . Unexpectedly , the transcriptional activation of egfp was markedly suppressed by adding NaCl that does not significantly affect fungal growth . As suggested by these findings , wild-type F graminearum that formed aerial hyphae on YG agar plates produced trichothecenes and the production was effectively suppressed by adding 1% NaCl to the agar . To evaluate the effects of abiotic stress on the expression of trichothecene biosynthesis ( Tri ) genes , a sensitive plate assay was established using GYEP medium ( which very weakly induces trichothecene production ) solidified with gellan gum . Using this assay , triazole fungicides were shown to cause transcriptional activation of egfp at sublethal concentrations . Indeed , trichothecene production significantly increased when F graminearum was grown in rice medium ( which moderately induces trichothecene ) amended with low doses of tebuconazole . The real-time monitoring system described here may help predict the risks of trichothecene contamination by the fungus under various environmental conditions . [ Sen. 2, subscore: 1.00 ]: Fusarium graminearum was engineered for expression of enhanced green fluorescent protein gene ( egfp ) as a reporter regulated in a manner similar to Tri5 , a key pathway gene in trichothecene biosynthesis . Using the transgenic fungus , it was found that the reporter gene was induced to express in aerial hyphae developed on trichothecene noninducing medium YG solidified by agar . Unexpectedly , the transcriptional activation of egfp was markedly suppressed by adding NaCl that does not significantly affect fungal growth . As suggested by these findings , wild-type F graminearum that formed aerial hyphae on YG agar plates produced trichothecenes and the production was effectively suppressed by adding 1% NaCl to the agar . To evaluate the effects of abiotic stress on the expression of trichothecene biosynthesis ( Tri ) genes , a sensitive plate assay was established using GYEP medium ( which very weakly induces trichothecene production ) solidified with gellan gum . Using this assay , triazole fungicides were shown to cause transcriptional activation of egfp at sublethal concentrations . Indeed , trichothecene production significantly increased when F graminearum was grown in rice medium ( which moderately induces trichothecene ) amended with low doses of tebuconazole . The real-time monitoring system described here may help predict the risks of trichothecene contamination by the fungus under various environmental conditions . [ Sen. 4, subscore: 1.00 ]: Fusarium graminearum was engineered for expression of enhanced green fluorescent protein gene ( egfp ) as a reporter regulated in a manner similar to Tri5 , a key pathway gene in trichothecene biosynthesis . Using the transgenic fungus , it was found that the reporter gene was induced to express in aerial hyphae developed on trichothecene noninducing medium YG solidified by agar . Unexpectedly , the transcriptional activation of egfp was markedly suppressed by adding NaCl that does not significantly affect fungal growth . As suggested by these findings , wild-type F graminearum that formed aerial hyphae on YG agar plates produced trichothecenes and the production was effectively suppressed by adding 1% NaCl to the agar . To evaluate the effects of abiotic stress on the expression of trichothecene biosynthesis ( Tri ) genes , a sensitive plate assay was established using GYEP medium ( which very weakly induces trichothecene production ) solidified with gellan gum . Using this assay , triazole fungicides were shown to cause transcriptional activation of egfp at sublethal concentrations . Indeed , trichothecene production significantly increased when F graminearum was grown in rice medium ( which moderately induces trichothecene ) amended with low doses of tebuconazole . The real-time monitoring system described here may help predict the risks of trichothecene contamination by the fungus under various environmental conditions . [ Sen. 6, subscore: 1.00 ]: Fusarium graminearum was engineered for expression of enhanced green fluorescent protein gene ( egfp ) as a reporter regulated in a manner similar to Tri5 , a key pathway gene in trichothecene biosynthesis . Using the transgenic fungus , it was found that the reporter gene was induced to express in aerial hyphae developed on trichothecene noninducing medium YG solidified by agar . Unexpectedly , the transcriptional activation of egfp was markedly suppressed by adding NaCl that does not significantly affect fungal growth . As suggested by these findings , wild-type F graminearum that formed aerial hyphae on YG agar plates produced trichothecenes and the production was effectively suppressed by adding 1% NaCl to the agar . To evaluate the effects of abiotic stress on the expression of trichothecene biosynthesis ( Tri ) genes , a sensitive plate assay was established using GYEP medium ( which very weakly induces trichothecene production ) solidified with gellan gum . Using this assay , triazole fungicides were shown to cause transcriptional activation of egfp at sublethal concentrations . Indeed , trichothecene production significantly increased when F graminearum was grown in rice medium ( which moderately induces trichothecene ) amended with low doses of tebuconazole . The real-time monitoring system described here may help predict the risks of trichothecene contamination by the fungus under various environmental conditions . [ Sen. 8, subscore: 1.00 ]: Fusarium graminearum was engineered for expression of enhanced green fluorescent protein gene ( egfp ) as a reporter regulated in a manner similar to Tri5 , a key pathway gene in trichothecene biosynthesis . Using the transgenic fungus , it was found that the reporter gene was induced to express in aerial hyphae developed on trichothecene noninducing medium YG solidified by agar . Unexpectedly , the transcriptional activation of egfp was markedly suppressed by adding NaCl that does not significantly affect fungal growth . As suggested by these findings , wild-type F graminearum that formed aerial hyphae on YG agar plates produced trichothecenes and the production was effectively suppressed by adding 1% NaCl to the agar . To evaluate the effects of abiotic stress on the expression of trichothecene biosynthesis ( Tri ) genes , a sensitive plate assay was established using GYEP medium ( which very weakly induces trichothecene production ) solidified with gellan gum . Using this assay , triazole fungicides were shown to cause transcriptional activation of egfp at sublethal concentrations . Indeed , trichothecene production significantly increased when F graminearum was grown in rice medium ( which moderately induces trichothecene ) amended with low doses of tebuconazole . The real-time monitoring system described here may help predict the risks of trichothecene contamination by the fungus under various environmental conditions .
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