| 29 matches found in 14 documents. Search time: 0.229 seconds. |
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Score: 6.00 |
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| Title: Overrepresentation of the proarrhythmic , sudden death predisposing sodium channel polymorphism S1103Y in a population-based cohort of African-American sudden infant death syndrome .
| | Author: Van Norstrand DW Tester DJ Ackerman MJ | | Journal: Heart Rhythm Citation: V : 5 P : 712-5 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18452875 | | Abstract: BACKGROUND : The S1103Y-SCN5A polymorphism has been implicated as a proarrhythmic , sudden death predisposing risk factor in African Americans , including one postmortem investigation of African-American infants with sudden infant death syndrome ( SIDS ) .
OBJECTIVE : The purpose of this study was to assess whether the relatively African-American-specific common polymorphism S1103Y in the SCN5A-encoded cardiac sodium channel is overrepresented in SIDS among African Americans .
METHODS : Seventy-one cases from a population-based cohort of unexplained infant deaths among African Americans ( 37 females and 34 males , average age 3 +/- 2 months , age range birth to 11 months ) were submitted to the Mayo Clinic Windland Smith Rice Sudden Death Genomics Laboratory for postmortem genetic testing .
Polymerase chain reaction and a restriction digest assay were performed to genotype this cohort for S1103Y .
RESULTS : Targeted mutational analysis of exon 18 in SCN5A of the African-American SIDS cohort ( n = 71 ) revealed the S1103Y polymorphism in 16 ( 22 . 5% ) of 71 African-American cases of SIDS compared to 135 ( 11 . 6% ) of 1 , 161 ostensibly healthy adult African Americans ( P = . 01 ) .
CONCLUSION : This study provides an independent assessment of the prevalence of S1103Y-SCN5A among African-American infants with sudden , unexpected , unexplained death prior to their first birthday .
Further scrutiny and quantification of the risk apparently associated with S1103Y appear warranted .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: BACKGROUND : The S1103Y-SCN5A polymorphism has been implicated as a proarrhythmic , sudden death predisposing risk factor in African Americans , including one postmortem investigation of African-American infants with sudden infant death syndrome ( SIDS ) .
[ Sen. 2, subscore: 1.00 ]: OBJECTIVE : The purpose of this study was to assess whether the relatively African-American-specific common polymorphism S1103Y in the SCN5A-encoded cardiac sodium channel is overrepresented in SIDS among African Americans .
[ Sen. 4, subscore: 1.00 ]: Polymerase chain reaction and a restriction digest assay were performed to genotype this cohort for S1103Y .
[ Sen. 5, subscore: 1.00 ]: RESULTS : Targeted mutational analysis of exon 18 in SCN5A of the African-American SIDS cohort ( n = 71 ) revealed the S1103Y polymorphism in 16 ( 22 . 5% ) of 71 African-American cases of SIDS compared to 135 ( 11 . 6% ) of 1 , 161 ostensibly healthy adult African Americans ( P = . 01 ) .
[ Sen. 6, subscore: 1.00 ]: CONCLUSION : This study provides an independent assessment of the prevalence of S1103Y-SCN5A among African-American infants with sudden , unexpected , unexplained death prior to their first birthday .
[ Sen. 7, subscore: 1.00 ]: Further scrutiny and quantification of the risk apparently associated with S1103Y appear warranted .
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Score: 5.00 |
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| Title: Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12 .
| | Author: Suzuki N Harada M Kusano T | | Journal: J Gen . Virol . Citation: V : 72 ( Pt 9 ) ( ) P : 2233-7 Year: 1991 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1840611 | | Abstract: The complete nucleotide sequence of rice dwarf phytoreovirus ( RDV ) genome segment S11 was determined .
S11 is 1067 nucleotides long .
There is an inverted repeat of 10 bp adjacent to the conserved 5-terminal hexanucleotide ( 5 GGUAAA 3 ) and 3-terminal tetranucleotide ( 5 UAGU 3 ) sequences .
A single large open reading frame found in the plus strand of S11 begins with the first AUG codon ( bases 6 to 8 ) and extends for 567 bases .
Evolutionary relatedness between RDV S11 and wound tumour phytoreovirus S12 based on amino acid sequence similarity ( 25 . 8% ) was found .
In addition to the first AUG triplet , RDV S11 possesses a second in-phase AUG triplet ( positions 30 to 32 ) nearby , which conforms to the Kozak consensus sequence .
Two forms of the protein were identified by using an in vitro transcription and translation system in which a tailored full-length cDNA was the initial template .
The abolition of the first AUG codon by site-directed mutagenesis resulted in disappearance of the larger translation product .
These results strongly suggest that the two products are translated from the first and second AUG codons .
Whether the two proteins are expressed in vivo is at present unclear . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The complete nucleotide sequence of rice dwarf phytoreovirus ( RDV ) genome segment S11 was determined .
[ Sen. 2, subscore: 1.00 ]: S11 is 1067 nucleotides long .
[ Sen. 4, subscore: 1.00 ]: A single large open reading frame found in the plus strand of S11 begins with the first AUG codon ( bases 6 to 8 ) and extends for 567 bases .
[ Sen. 5, subscore: 1.00 ]: Evolutionary relatedness between RDV S11 and wound tumour phytoreovirus S12 based on amino acid sequence similarity ( 25 . 8% ) was found .
[ Sen. 6, subscore: 1.00 ]: In addition to the first AUG triplet , RDV S11 possesses a second in-phase AUG triplet ( positions 30 to 32 ) nearby , which conforms to the Kozak consensus sequence .
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Score: 3.00 |
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| Title: Sequence analysis of Pns11 , a nonstructural protein of rice gall dwarf virus , and its expression and detection in infected rice plants and vector insects .
| | Author: Moriyasu Y Ishikawa K Kikuchi A Imanishi S Tomita S Akutsu K Omura T | | Journal: Virus Genes Citation: V : 20 ( 3 ) P : 237-41 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10949951 | | Abstract: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined .
The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses , which are other members of Phytoreovirus .
A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA .
An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects .
However , the antiserum did not react with purified viral proteins .
These results suggest that S11 encodes a nonstructural protein of RGDV .
This protein was named Pns11 . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined .
[ Sen. 3, subscore: 1.00 ]: A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA .
[ Sen. 6, subscore: 1.00 ]: These results suggest that S11 encodes a nonstructural protein of RGDV .
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Score: 3.00 |
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| Title: Targeting presequence acquisition after mitochondrial gene transfer to the nucleus occurs by duplication of existing targeting signals .
| | Author: Kadowaki K Kubo N Ozawa K Hirai A | | Journal: EMBO J Citation: V : 15 ( 23 ) P : 6652-61 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8978691 | | Abstract: We have cloned a gene for mitochondrial ribosomal protein S11 ( RPS11 ) , which is encoded in lower plants by the mitochondrial genome , in higher plants by the nuclear genome , demonstrating genetic information transfer from the mitochondrial genome to the nucleus during flowering plant evolution .
The sequence s11-1 encodes an N-terminal extension as well as an organelle-derived RPS11 region .
Surprisingly , the N-terminal region has high amino acid sequence similarity with the presequence of the beta-subunit of ATP synthase from plant mitochondria , suggesting a common lineage of the presequences .
The deduced N-terminal region of s11-2 , a second nuclear-encoded homolog of rps11 , shows high sequence similarity with the putative presequence of cytochrome oxidase subunit Vb .
The sharing of the N-terminal region together with its 5 flanking untranslated nucleotide sequence in different proteins strongly suggests an involvement of duplication/recombination for targeting signal acquisition after gene migration .
A remnant of ancestral rps11 sequence , transcribed and subjected to RNA editing , is found in the mitochondrial genome , indicating that inactivation of mitochondrial rps11 gene expression was initiated at the translational level prior to termination of transcription . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: We have cloned a gene for mitochondrial ribosomal protein S11 ( RPS11 ) , which is encoded in lower plants by the mitochondrial genome , in higher plants by the nuclear genome , demonstrating genetic information transfer from the mitochondrial genome to the nucleus during flowering plant evolution .
[ Sen. 2, subscore: 1.00 ]: The sequence s11-1 encodes an N-terminal extension as well as an organelle-derived RPS11 region .
[ Sen. 4, subscore: 1.00 ]: The deduced N-terminal region of s11-2 , a second nuclear-encoded homolog of rps11 , shows high sequence similarity with the putative presequence of cytochrome oxidase subunit Vb .
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Score: 2.00 |
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| Title: Molecular analysis of six segments of Tobacco leaf enation virus , a novel phytoreovirus from tobacco | | Author: Picton A Potgieter C Rey ME .
| | Journal: Citation: V : ( ) P : Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17356907 | | Abstract: Tobacco leaf enation virus ( TLEV ) is a putative member of the genus Phytoreovirus within the family Reoviridae .
Previous western blot analysis of structural viral proteins ( apparent molecular weights of 93 kDa ; 58 kDa ; 48 kDa ; 39 kDa and 36 kDa ) associated with TLEV , isolated from infected tobacco in South Africa , suggested that these proteins may correspond to structural Wound tumor virus ( WTV ) proteins .
To further establish the nature of this novel virus disease phenotype in tobacco , molecular characterization of six dsRNA components was undertaken .
Full-length cDNA clones were obtained by an optimized modified single-primer amplification sequence-independent dsRNA cloning method .
Results of this study revealed the conserved terminal sequence : 5GG ( U/C ) . . . UGAU 3 of segments S6-S12 , while adjacent to these conserved terminal sequences are imperfect inverted repeats ( 7-15 bp in length ) , both features being common to reoviruses .
The complete nucleotide sequences of segments S5 ( 2 , 610 bp ) , S7 ( 1 , 740 bp ) , S8 ( 1 , 439 bp ) , S10 ( 1 , 252 bp ) , S11 ( 1 , 187 bp ) and S12 ( 836 bp ) were determined .
Comparison of full-length nucleotide sequences with corresponding segments of other phytoreoviruses , Rice gall dwarf virus ( RGDV ) , Rice dwarf virus ( RDV ) and WTV has shown nucleotide and predicted amino acid identities within the range of 30-60% .
TLEV consistently shows a higher identity to WTV than to other phytoreovirus species where sequence data is available .
Each segment had a single predicted open reading frame encoding proteins with calculated molecular weights of S5 ( 90 . 6 kDa ) ; S7 ( 58 . 1 kDa ) ; S8 ( 47 . 7 kDa ) ; S10 ( 39 . 8 kDa ) ; S11 ( 35 kDa ) and S12 ( 19 . 5 kDa ) .
The relatively low nucleotide and amino acid identity to other members of the genus demonstrates that TLEV is a novel phytoreovirus , distinct from the only other reported dicotyledenous-infecting WTV and is the first phytoreovirus reported to emerge in Africa .
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[ Sen. 6, subscore: 1.00 ]: The complete nucleotide sequences of segments S5 ( 2 , 610 bp ) , S7 ( 1 , 740 bp ) , S8 ( 1 , 439 bp ) , S10 ( 1 , 252 bp ) , S11 ( 1 , 187 bp ) and S12 ( 836 bp ) were determined .
[ Sen. 9, subscore: 1.00 ]: Each segment had a single predicted open reading frame encoding proteins with calculated molecular weights of S5 ( 90 . 6 kDa ) ; S7 ( 58 . 1 kDa ) ; S8 ( 47 . 7 kDa ) ; S10 ( 39 . 8 kDa ) ; S11 ( 35 kDa ) and S12 ( 19 . 5 kDa ) .
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Score: 2.00 |
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| Title: Immunodetection of rice dwarf phytoreoviral proteins in both insect and plant hosts .
| | Author: Suzuki N Sugawara M Kusano T Mori H Matsuura Y | | Journal: Virology Citation: V : 202 ( 1 ) P : 41-8 Year: 1994 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8009852 | | Abstract: Peptides encoded by truncated ( S1 and S2 ) or full-length ( S3 to S11 ) cDNAs of 11 of the 12 rice dwarf phytoreovirus ( RDV ) genome segments were expressed in a baculovirus vector system .
Antibodies raised against each of the expressed peptides were used as probes to detect the authentic proteins encoded by the RDV open reading frame .
Polypeptides identified as gene products of S1 to S11 in both RDV-infected rice leaf and leafhopper ( Nephotettix cincticeps ) homogenates were the P1 minor core ( 170 kDa ) , P2 ( 130 kDa ) , P3 major core ( 110 kDa ) , Pns9 nonstructural ( 83 kDa ) , P5 ( 89 kDa ) , Pns6 nonstructural ( 56 kDa ) , P7 minor core ( 58 kDa ) , P8 outercapsid ( 43 kDA ) , Pns9 nonstructural ( 49 kDa ) , Pns10 nonstructural ( 35 kDa ) , and Pns11a nonstructural ( 23 kDa ) proteins .
These molecular masses were in accord with those obtained from previous in vitro translation analysis .
The locations of P2 and P5 remain to be determined although both of these are assumed to be outer layer proteins .
Quantitative detection showed that accumulation ( per gram of total proteins ) of the virus-coded proteins in rice leaves is much greater ( more than 15 times ) than that in leafhoppers and that the content of the individual proteins varies within a sample from rice or leafhopper and also varies among different samples . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Peptides encoded by truncated ( S1 and S2 ) or full-length ( S3 to S11 ) cDNAs of 11 of the 12 rice dwarf phytoreovirus ( RDV ) genome segments were expressed in a baculovirus vector system .
[ Sen. 3, subscore: 1.00 ]: Polypeptides identified as gene products of S1 to S11 in both RDV-infected rice leaf and leafhopper ( Nephotettix cincticeps ) homogenates were the P1 minor core ( 170 kDa ) , P2 ( 130 kDa ) , P3 major core ( 110 kDa ) , Pns9 nonstructural ( 83 kDa ) , P5 ( 89 kDa ) , Pns6 nonstructural ( 56 kDa ) , P7 minor core ( 58 kDa ) , P8 outercapsid ( 43 kDA ) , Pns9 nonstructural ( 49 kDa ) , Pns10 nonstructural ( 35 kDa ) , and Pns11a nonstructural ( 23 kDa ) proteins .
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Score: 1.00 |
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| Title: Two whitebacked planthopper resistance genes in rice share the same loci with those for brown planthopper resistance .
| | Author: Tan GX Weng QM Ren X Huang Z Zhu LL He GC .
| | Journal: Heredity Citation: V : 92 ( 3 ) P : 212-7 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14666132 | | Abstract: The whitebacked planthopper ( WBPH ) , Sogatella furcifera , and brown planthopper ( BPH ) Nilaparvata lugens Stl are important sucking insects of rice ( Oryza sativa L ) crops throughout the world .
Rice B5 , which has derived its resistance genes from the wild rice O officinalis Wall ex Watt , is a line that is highly resistant to both WBPH and BPH .
Previously , two resistance genes against BPH , Qbp1 , and Qbp2 in B5 had been mapped onto chromosome 3 and chromosome 4 , respectively .
In this study , we employed a mapping population composed of 187 recombinant inbred lines ( RILs ) , produced from a cross between B5 and susceptible variety Minghui63 , to locate the WBPH and BPH resistance genes .
A RFLP survey of the bulked extremes from the RIL population identified two genomic regions , one on chromosome 3 and the other on chromosome 4 , likely containing the resistance genes to planthoppers .
QTL analysis of the RILs further confirmed that two WBPH resistance genes were mapped on the same loci as Qbp1 and Qbp2 , using a linkage map with 242 molecular markers distributed on 12 rice chromosomes .
Of the two WBPH resistance genes , one designated Wbph7 ( t ) was located within a 1 . 1-cM region between R1925 and G1318 on chromosome 3 , the other designated Wbph8 ( t ) was within a 0 . 3-cM region flanked by R288 and S11182 on chromosome 4 .
A two-way analysis of variance showed that two loci acted independently with each other in determining WBPH resistance .
The results have significant implications in studying the interactions between sucking insects and plants and in breeding programs of resistance to rice planthoppers . | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Of the two WBPH resistance genes , one designated Wbph7 ( t ) was located within a 1 . 1-cM region between R1925 and G1318 on chromosome 3 , the other designated Wbph8 ( t ) was within a 0 . 3-cM region flanked by R288 and S11182 on chromosome 4 .
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Score: 1.00 |
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| Title: High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice ( Oryza sativa L ) .
| | Author: Yang H You A Yang Z Zhang F He R Zhu L He G | | Journal: Theor . Appl . Genet . Citation: V : 110 ( 1 ) P : 182-91 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15549231 | | Abstract: Resistance to the brown planthopper ( BPH ) , Nilaparvata lugens Stal , a devastating sucking insect pest of rice , is an important breeding objective in rice improvement programs .
Bph15 , one of the 17 major BPH resistance genes so far identified in both cultivated and wild rice , has been identified in an introgression line , B5 , and mapped on chromosome 4 flanked by restriction fragment length polymorphism markers C820 and S11182 .
In order to pave the way for positional cloning of this gene , we have developed a high-resolution genetic map of Bph15 by positioning 21 DNA markers in the target chromosomal region .
Mapping was based on a PCR-based screening of 9 , 472 F ( 2 ) individuals derived from a cross between RI93 , a selected recombinant inbred line of B5 bearing the resistance gene Bph15 , and a susceptible variety , Taichung Native 1 , in order to identify recombinant plants within the Bph15 region .
Recombinant F ( 2 ) individuals with the Bph15 genotype were determined by phenotype evaluation .
Analysis of recombination events in the Bph15 region delimited the gene locus to an interval between markers RG1 and RG2 that co-segregated with the M1 marker .
A genomic library of B5 was screened using these markers , and bacterial artificial chromosome clones spanning the Bph15 chromosome region were obtained .
An assay of the recombinants using the sub-clones of these clones in combination with sequence analysis delimited the Bph15 gene to a genomic segment of approximately 47 kb .
This result should serve as the basis for eventual isolation of the Bph15 resistance gene . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Bph15 , one of the 17 major BPH resistance genes so far identified in both cultivated and wild rice , has been identified in an introgression line , B5 , and mapped on chromosome 4 flanked by restriction fragment length polymorphism markers C820 and S11182 .
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Score: 1.00 |
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| Title: Molecular characterization of the largest and smallest genome segments , S1 and S12 , of Rice gall dwarf virus .
| | Author: Zhang HM Yang J Xin X Chen JP Adams MJ | | Journal: Virus Genes Citation: V : 35 P : 815-23 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17674177 | | Abstract: The nucleotide sequences of segments S1 and S12 of a Chinese isolate of Rice gall dwarf virus ( RGDV ) were determined .
This provides the first complete sequences of these segments .
The complete sequence of S1 , the largest genome segment of RGDV , was 4 , 505 nucleotides in length and was predicted to encode a large protein of 1 , 458 amino acids with a calculated molecular mass of nearly 166 . 2 kDa .
The protein was related to that encoded by S1 of Rice dwarf virus ( RDV ; 50% identity and 67% similarity ) and ( to a lesser extent ) to some large proteins of other reoviruses .
It appears to be an RNA-dependent RNA polymerase ( RdRp ) and is probably present in particles as a minor core protein .
S12 , the smallest genome segment of RGDV , was 853 nucleotides in length , encoding a single major protein of 206 amino acids with a calculated molecular mass of nearly 23 . 6 kDa .
This protein , though a little larger than those of RDV S11 and Wound tumor virus ( WTV ) S12 in size , showed some similarity to them , especially in the conserved N-terminal region and may have RNA-binding properties .
Despite having a common host plant , RDV and RGDV were not more closely related to one another than either of them was to WTV .
Phylogenetic analysis of the RdRp showed that members of the genus Phytoreovirus were more closely related to those of the genus Rotavirus than to any other genus within the family Reoviridae .
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[ Sen. 7, subscore: 1.00 ]: This protein , though a little larger than those of RDV S11 and Wound tumor virus ( WTV ) S12 in size , showed some similarity to them , especially in the conserved N-terminal region and may have RNA-binding properties .
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Score: 1.00 |
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| Title: Nucleotide sequences of genome segments S8 , encoding a capsid protein , and S10 , encoding a 36K protein , of rice gall dwarf virus .
| | Author: Noda H Ishikawa K Hibino H Kato H Omura T | | Journal: J Gen . Virol . Citation: V : 72 ( Pt 11 ) ( ) P : 2837-42 Year: 1991 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1940872 | | Abstract: The nucleotide sequences of DNAs complementary to the eighth ( S8 ) and the tenth ( S10 ) largest of the 12 genome segments of rice gall dwarf virus ( RGDV ) were determined .
The S8 and S10 segments consist of 1578 and 1198 nucleotides , each with a single open reading frame extending for 1278 nucleotides from nucleotide 21 , and 960 nucleotides from nucleotide 22 , respectively .
S8 encodes a polypeptide of 426 amino acids with an Mr of 47419 .
The amino acid sequences of several peptide fragments of the major outer capsid protein reported as 45K were contained in the predicted polypeptide .
This protein , renamed the 47K protein , showed high homology with the outer capsid proteins of rice dwarf virus ( RDV ) and wound tumour virus ( WTV ) ; there was 56 , 52 and 48% amino acid sequence identity between RGDV and WTV , RGDV and RDV , and RDV and WTV , respectively .
S10 had the coding potential for a polypeptide of 320 amino acids with an Mr of 36 , 095 ( 36K protein ) , which exhibits 32% and 35% amino acid sequence identity with the predicted translation product of RDV S9 and the P9 capsid protein encoded by WTV S11 , respectively .
The conserved terminal sequences 5 GG . . . GAU 3 which are present in all genome segments of WTV and RDV so far analysed , and in S9 of RGDV , were also found in RGDV S8 and S10 .
This conserved sequence together with the segment-specific inverted repeats found in the terminal sequence of RGDV S8 and S10 are thus characteristic structures common to all three phytoreoviruses .
The nucleotide sequence of the region surrounding the inverted repeats was more similar between RGDV and WTV than between RGDV and RDV . | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: S10 had the coding potential for a polypeptide of 320 amino acids with an Mr of 36 , 095 ( 36K protein ) , which exhibits 32% and 35% amino acid sequence identity with the predicted translation product of RDV S9 and the P9 capsid protein encoded by WTV S11 , respectively .
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Score: 1.00 |
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| Title: Nucleotide sequence of rice dwarf phytoreovirus genome segment 2 : completion of sequence analyses of rice dwarf virus .
| | Author: Uyeda I Suda N Yamada N Kudo H Murao K Suga H Kimura I Shikata E Kitagawa Y Kusano T | | Journal: Intervirology Citation: V : 37 ( 1 ) P : 6-11 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub7928290 | | Abstract: The complete nucleotide sequence of rice dwarf phytoreovirus genome segment 2 ( S2 ) was determined to be 3 , 512 nucleotides long with one open reading frame initiating at nucleotide 15 and terminating at nucleotide 3363 .
The encoded polypeptide was predicted to have 1 , 116 residues with a relative molecular weight of 123 kD .
Comparison of S2 of two isolates showed they had identical lengths and 97 and 98 . 3% nucleotide and amino acid sequence identities , respectively .
A search of the Swiss-Prot data base ( R 22 . 0 ) failed to find any proteins with significant homology to the S2-encoded protein .
Determination of the nucleotide sequence of the S2 has completed the sequence determination of the genome of rice dwarf virus .
Homology searches made for proteins encoded by each of the genomic segments showed that the polypeptide encoded by S11 has similarity to histone H1 protein and VP6 of blue tongue virus , indicating it might possess nucleic acid binding properties . | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Homology searches made for proteins encoded by each of the genomic segments showed that the polypeptide encoded by S11 has similarity to histone H1 protein and VP6 of blue tongue virus , indicating it might possess nucleic acid binding properties .
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Score: 1.00 |
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| Title: Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12 .
| | Author: Suzuki N Harada M Kusano T | | Journal: J Gen . Virol . Citation: V : 72 ( Pt 9 ) ( ) P : 2233-7 Year: 1991 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub1840611 | | Abstract: The complete nucleotide sequence of rice dwarf phytoreovirus ( RDV ) genome segment S11 was determined .
S11 is 1067 nucleotides long .
There is an inverted repeat of 10 bp adjacent to the conserved 5-terminal hexanucleotide ( 5 GGUAAA 3 ) and 3-terminal tetranucleotide ( 5 UAGU 3 ) sequences .
A single large open reading frame found in the plus strand of S11 begins with the first AUG codon ( bases 6 to 8 ) and extends for 567 bases .
Evolutionary relatedness between RDV S11 and wound tumour phytoreovirus S12 based on amino acid sequence similarity ( 25 . 8% ) was found .
In addition to the first AUG triplet , RDV S11 possesses a second in-phase AUG triplet ( positions 30 to 32 ) nearby , which conforms to the Kozak consensus sequence .
Two forms of the protein were identified by using an in vitro transcription and translation system in which a tailored full-length cDNA was the initial template .
The abolition of the first AUG codon by site-directed mutagenesis resulted in disappearance of the larger translation product .
These results strongly suggest that the two products are translated from the first and second AUG codons .
Whether the two proteins are expressed in vivo is at present unclear . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Overrepresentation of the proarrhythmic , sudden death predisposing sodium channel polymorphism S1103Y in a population-based cohort of African-American sudden infant death syndrome .
| | Author: Van Norstrand DW Tester DJ Ackerman MJ | | Journal: Heart Rhythm Citation: V : 5 P : 712-5 Year: 2008 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub18452875 | | Abstract: BACKGROUND : The S1103Y-SCN5A polymorphism has been implicated as a proarrhythmic , sudden death predisposing risk factor in African Americans , including one postmortem investigation of African-American infants with sudden infant death syndrome ( SIDS ) .
OBJECTIVE : The purpose of this study was to assess whether the relatively African-American-specific common polymorphism S1103Y in the SCN5A-encoded cardiac sodium channel is overrepresented in SIDS among African Americans .
METHODS : Seventy-one cases from a population-based cohort of unexplained infant deaths among African Americans ( 37 females and 34 males , average age 3 +/- 2 months , age range birth to 11 months ) were submitted to the Mayo Clinic Windland Smith Rice Sudden Death Genomics Laboratory for postmortem genetic testing .
Polymerase chain reaction and a restriction digest assay were performed to genotype this cohort for S1103Y .
RESULTS : Targeted mutational analysis of exon 18 in SCN5A of the African-American SIDS cohort ( n = 71 ) revealed the S1103Y polymorphism in 16 ( 22 . 5% ) of 71 African-American cases of SIDS compared to 135 ( 11 . 6% ) of 1 , 161 ostensibly healthy adult African Americans ( P = . 01 ) .
CONCLUSION : This study provides an independent assessment of the prevalence of S1103Y-SCN5A among African-American infants with sudden , unexpected , unexplained death prior to their first birthday .
Further scrutiny and quantification of the risk apparently associated with S1103Y appear warranted .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Overrepresentation of the proarrhythmic , sudden death predisposing sodium channel polymorphism S1103Y in a population-based cohort of African-American sudden infant death syndrome .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Rice dwarf phytoreovirus segment S11 encodes a nucleic acid binding protein .
| | Author: Xu H Li Y Mao Z Li Y Wu Z Qu L An C Ming X Schiemann J Casper R Chen Z | | Journal: Virology Citation: V : 240 ( 2 ) P : 267-72 Year: 1998 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub9454700 | | Abstract: The function of rice dwarf virus segment 11 and the corresponding segments of other phytoreoviruses is not yet determined .
The amino acid sequence of Pns11 , encoded by segment 11 , contains a putative zinc finger and five flanking basic regions at the C-terminus .
The full-length Pns11 protein and three truncated derivatives , which lack the N-terminus , the zinc-finger or the C-terminal five basic regions were expressed in Escherichia coli and their nucleic acid binding properties were studied .
Pns11 interacts with single and double-stranded forms of DNA and RNA in a sequence-nonspecific manner .
The truncated derivative which contains both the zinc-finger and the C-terminal basic regions has the same binding properties as the full-length Pns11 .
However , removal of either of these domains prevents binding activity .
The binding activity of Pns11 was drastically reduced when the blots were treated with a high concentration of EDTA .
Moreover , Pns11 extracted from infected rice also binds to single-stranded RNA .
These data suggest that RDV Pns11 binding activity is structure-dependent and it may play an important role in virus replication and/or genome assortment .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice dwarf phytoreovirus segment S11 encodes a nucleic acid binding protein .
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