423 matches found in 290 documents. Search time: 0.582 seconds. |
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Score: 9.00 | | Author: Prasad K Kushalappa K Vijayraghavan U | Journal: Mech . Dev . Citation: V : 120 ( 4 ) P : 491-502 Year: 2003 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12676326 Accession (PMID): 12676326 | Matching Sentences: [ Sen. 10, subscore: 2.00 ]: By sequence comparison of RFL upstream sequences , intron1 , intron2 and the Arabidopsis LFY promoter , we identify putative cis-regulatory elements unique to RFL . [ Sen. 2, subscore: 1.00 ]: The spatial and temporal RNA expression pattern of a rice LFY-like gene : RFL differs significantly from that in several other species studied thus far . [ Sen. 3, subscore: 1.00 ]: The onset of RFL expression coincides with inflorescence meristem ( panicle meristem ) initiation , and continues during panicle branching . [ Sen. 5, subscore: 1.00 ]: To explore mechanisms underlying this distinct expression pattern , we have tested the transcription regulatory functions of sequences upstream to RFL coding sequences either alone , or together with intragenic segments . [ Sen. 6, subscore: 1.00 ]: Sequences 3 . 0 kb upstream of the RFL reading frame do not confer correctly regulated reporter gene expression in transgenic rice . [ Sen. 7, subscore: 1.00 ]: In contrast , RFL intron1 or 2 can individually confer the expected profile in the developing panicle and floret . [ Sen. 9, subscore: 1.00 ]: Strikingly , RFL intron2 sequences can even utilize the Arabidopsis thaliana LFY promoter to confer regulated transcription in young rice panicles . [ Sen. 11, subscore: 1.00 ]: These data exemplify the use of regulatory circuits specific to rice RFL that may underlie its divergent function .
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Score: 9.00 | | Author: Rao NN Prasad K Kumar PR Vijayraghavan U | Journal: Proc Natl Acad Sci U S A Citation: V : 105 P : 3646-51 Year: 2008 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub18305171 Accession (PMID): 18305171 | Matching Sentences: [ Sen. 2, subscore: 1.00 ]: In Arabidopsis thaliana , a model eudicot species , the transcription factor LFY confers a floral fate to new meristems arising from the periphery of the reproductive shoot apex Diverse orthologous LFY genes regulate vegetative-to-reproductive phase transition when expressed in Arabidopsis , a property not shared by RFL , the homolog in the agronomically important grass , rice . [ Sen. 3, subscore: 1.00 ]: We have characterized RFL by knockdown of its expression and by its ectopic overexpression in transgenic rice . [ Sen. 4, subscore: 1.00 ]: We find that reduction in RFL expression causes a dramatic delay in transition to flowering , with the extreme phenotype being no flowering . [ Sen. 5, subscore: 1.00 ]: Conversely , RFL overexpression triggers precocious flowering . [ Sen. 6, subscore: 1.00 ]: In these transgenics , the expression levels of known flowering time genes reveal RFL as a regulator of OsSOC1 ( OsMADS50 ) , an activator of flowering . [ Sen. 7, subscore: 1.00 ]: Aside from facilitating a transition of the main growth axis to an inflorescence meristem , RFL expression status affects vegetative axillary meristems and therefore regulates tillering . [ Sen. 8, subscore: 1.00 ]: The unique spatially and temporally regulated RFL expression during the development of vegetative axillary bud ( tiller ) primordia and inflorescence branch primordia is therefore required to produce tillers and panicle branches , respectively . [ Sen. 9, subscore: 1.00 ]: Our data provide mechanistic insights into a unique role for RFL in determining the typical rice plant architecture by regulating distinct downstream pathways . [ Sen. 10, subscore: 1.00 ]: These results offer a means to alter rice flowering time and plant architecture by manipulating RFL-mediated pathways .
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Score: 9.00 | | Author: Kyozuka J Konishi S Nemoto K Izawa T Shimamoto K | Journal: Proc . Natl . Acad . Sci . USA Citation: V : 95 ( 5 ) P : 1979-82 Year: 1998 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub9482818 Accession (PMID): 9482818 | Matching Sentences: [ Sen. 7, subscore: 2.00 ]: Expression pattern of RFL raised a possibility that , unlike FLO and LFY , RFL might be involved in panicle branching . [ Sen. 2, subscore: 1.00 ]: To examine whether same mechanisms control floral development in distantly related species such as grasses , we isolated RFL , FLO-LFY homolog of rice , and examined its expression and function . [ Sen. 3, subscore: 1.00 ]: Northern analysis showed that RFL is expressed predominantly in very young panicle but not in mature florets , mature leaves , or roots . [ Sen. 4, subscore: 1.00 ]: In situ hybridization revealed that RFL RNA was expressed in epidermal cells in young leaves at vegetative growth stage . [ Sen. 5, subscore: 1.00 ]: After the transition to reproductive stage , RFL RNA was detected in all layers of very young panicle including the apical meristem , but absent in the incipient primary branches . [ Sen. 6, subscore: 1.00 ]: As development of branches proceeds , RFL RNA accumulation localized in the developing branches except for the apical meristems of the branches and secondary branch primordia . [ Sen. 8, subscore: 1.00 ]: Transgenic Arabidopsis plants constitutively expressing RFL from the cauliflower mosaic virus 35S promoter were produced to test whether 35S-RFL would cause similar phenotype as observed in 35S-LFY plants . [ Sen. 10, subscore: 1.00 ]: Instead , development of cotyledons , rosette leaves , petals , and stamens was severely affected , demonstrating that RFL function is distinct from that of LFY .
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Score: 7.00 | | Author: Jena KK Kochert G | Journal: Plant Mol . Biol . Citation: V : 16 ( 5 ) P : 831-9 Year: 1991 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub1677594 Accession (PMID): 1677594 | Matching Sentences: [ Sen. 6, subscore: 2.00 ]: By comparing RFLPs from the allotetraploids with those from a CC genome diploid wild species ( O officinalis ) , it was possible to detect RFLPs specific for both the CC and DD genomes of the allotetraploid . [ Sen. 1, subscore: 1.00 ]: Restriction fragment length polymorphisms ( RFLPs ) were studied in fourteen accessions of CCDD genome allotetraploid wild rice species ( Oryza latifolia , O alta and O grandiglumis ) . [ Sen. 2, subscore: 1.00 ]: Fourteen nuclear RFLP markers previously mapped in AA genome-cultivated rice were used as probes . [ Sen. 3, subscore: 1.00 ]: A phylogenetic tree , constructed by parsimony analysis based on RFLPs , grouped the accessions according to their geographic origin from Central or South America . [ Sen. 5, subscore: 1.00 ]: Duplicate loci , representing the two constituent genomes of the allotetraploid , were observed for most RFLP markers . [ Sen. 7, subscore: 1.00 ]: In inter-accession F2 populations , independent segregation of RFLP markers for CC and DD genomes was observed .
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Score: 6.00 | | Author: Leach JE Rhoads ML Vera Cruz CM White FF Mew TW Leung H | Journal: Appl . Environ . Microbiol . Citation: V : 58 ( 7 ) P : 2188-95 Year: 1992 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub1353345 Accession (PMID): 1353345 | Matching Sentences: [ Sen. 3, subscore: 2.00 ]: Twenty-seven RFLP types were identified ; there was no overlap of RFLP types among the six races from the Philippines . [ Sen. 2, subscore: 1.00 ]: Genomic DNA from X oryzae pv . oryzae was digested with EcoRI and analyzed for restriction fragment length polymorphisms ( RFLPs ) with repetitive DNA element as a probe . [ Sen. 4, subscore: 1.00 ]: Most variability ( 20 RFLP types ) was found in strains of races 1 , 2 , and 3 , which were isolated from tropical lowland areas . [ Sen. 5, subscore: 1.00 ]: Four RFLP types ( all race 5 ) were found among strains isolated from cultivars grown in the temperate highlands . [ Sen. 8, subscore: 1.00 ]: Cluster analysis based on RFLP banding patterns showed five groupings at 85% similarity .
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