| 29 matches found in 16 documents. Search time: 0.521 seconds. |
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Score: 6.00 |
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| Title: Overexpression of Rab16A gene in indica rice variety for generating enhanced salt tolerance .
| | Author: Ganguly M Datta K Roychoudhury A Gayen D Sengupta DN Datta SK | | Journal: Plant Signal Behav Citation: V : 7 P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22499169 Accession (PMID): 22499169 | | Abstract: We report here the overexpression of Rab16A full length gene ( promoter + ORF ) , from the salt-tolerant indica rice Pokkali , in the salt-susceptible indica rice variety Khitish , via particle bombardment .
Molecular analysis of the transgenics revealed stable integration of the transgene upto T2 generation .
High level of expression of the transgene ( driven by its own stress-inducible promoter ) , as well as the protein , was detectable in the leaves under simulated salinity stress ( 250 mM NaCl , 24 h ) ; the expression level being higher than wild type ( WT ) plants .
The Rab16A transcript also increased gradually with seed maturity , with its maximal accumulation at 30 d after pollination ( DAP ) ie , fully matured seeds , explaining the protective role of Rab16A gene during seed maturation .
Enhanced tolerance to salinity was observed in the plants transformed with Rab16A .
The superior physiological performances of the transgenics under salt treatment were also reflected in lesser shoot or root length inhibition , reduced chlorophyll damages , lesser accumulation of Na ( + ) and reduced loss of K ( + ) , increased proline content as compared with the WT plants .
All these results indicated that the overproduction of RAB16A protein in the transgenics enable them to display enhanced tolerance to salinity stress with improved physiological traits .
Our work demonstrates the profound potential of Group 2 LEA proteins ( to which RAB16A belongs to ) in conferring stress tolerance in crop plants through their genetic manipulation .
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[ Sen. 4, subscore: 2.00 ]: The Rab16A transcript also increased gradually with seed maturity , with its maximal accumulation at 30 d after pollination ( DAP ) ie , fully matured seeds , explaining the protective role of Rab16A gene during seed maturation .
[ Sen. 1, subscore: 1.00 ]: We report here the overexpression of Rab16A full length gene ( promoter + ORF ) , from the salt-tolerant indica rice Pokkali , in the salt-susceptible indica rice variety Khitish , via particle bombardment .
[ Sen. 5, subscore: 1.00 ]: Enhanced tolerance to salinity was observed in the plants transformed with Rab16A .
[ Sen. 7, subscore: 1.00 ]: All these results indicated that the overproduction of RAB16A protein in the transgenics enable them to display enhanced tolerance to salinity stress with improved physiological traits .
[ Sen. 8, subscore: 1.00 ]: Our work demonstrates the profound potential of Group 2 LEA proteins ( to which RAB16A belongs to ) in conferring stress tolerance in crop plants through their genetic manipulation .
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Score: 5.00 |
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| Title: Transgenic tobacco plants overexpressing the heterologous lea gene Rab16A from rice during high salt and water deficit display enhanced tolerance to salinity stress .
| | Author: RoyChoudhury A Roy C Sengupta DN | | Journal: Plant Cell Rep Citation: V : 26 P : 1839-59 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17554543 Accession (PMID): 17554543 | | Abstract: The full length Rab16A , from the indica rice Pokkali , was introduced into tobacco by Agrobacterium-mediated transformation .
The transgene was stably integrated into the genome and they originated from different lines of integration .
Expression of Rab16A transcript driven by its own promoter ( stress inducible ) in T2 progenies , only when triggered by salinity/ABA/PEG ( Polyethylene glycol ) -mediated dehydration , but not at the constitutive level , led to the stress-induced accumulation of RAB16A protein in the leaves of transgenic plants .
The selected independent transgenic lines showed normal growth , morphology and seed production as the WT plants without any yield penalty under stress conditions .
They exhibited significantly increased tolerance to salinity , sustained growth rates under stress conditions ; with concomitant increased osmolyte production like reducing sugars , proline and higher polyamines .
They also showed delayed development of damage symptoms with better antioxidative machinery and more favorable mineral balance , as reflected by reduced H2O2 levels and lipid peroxidation , lesser chlorophyll loss as well as lesser accumulation of Na+ and greater accumulation of K+ in 200 mM NaCl .
These findings establish the potential role of Rab16A gene in conferring salt tolerance without affecting growth and yield , as well as pointing to the fact that the upstream region of Rab16A behaves as an efficient stress-inducible promoter .
Our result also suggests the considerable potential of Group 2 lea genes as molecular tools for genetic engineering of plants towards stress tolerance .
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[ Sen. 3, subscore: 2.00 ]: Expression of Rab16A transcript driven by its own promoter ( stress inducible ) in T2 progenies , only when triggered by salinity/ABA/PEG ( Polyethylene glycol ) -mediated dehydration , but not at the constitutive level , led to the stress-induced accumulation of RAB16A protein in the leaves of transgenic plants .
[ Sen. 7, subscore: 2.00 ]: These findings establish the potential role of Rab16A gene in conferring salt tolerance without affecting growth and yield , as well as pointing to the fact that the upstream region of Rab16A behaves as an efficient stress-inducible promoter .
[ Sen. 1, subscore: 1.00 ]: The full length Rab16A , from the indica rice Pokkali , was introduced into tobacco by Agrobacterium-mediated transformation .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Glucose modulates the abscisic acid-inducible Rab16A gene in cereal embryos .
| | Author: Toyofuku K Loreti E Vernieri P Alpi A Perata P Yamaguchi J | | Journal: Plant Mol . Biol . Citation: V : 42 ( 3 ) P : 451-60 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10798615 Accession (PMID): 10798615 | | Abstract: Glucose effects on the expression of the abscisic acid-inducible Rab16A gene were examined in rice and barley embryos .
Glucose feeding to rice embryos negatively affects the endogenous abscisic acid content and represses the promoter activity of the Rab16A gene .
Glucose repression of the Rab16A gene takes place both at a transcriptional and a post-transcriptional level .
Modulation of the abscisic acid content in rice embryos triggered by glucose did not directly influence the expression of the rice alpha-amylase gene RAmy3D , which is known to be under glucose control .
The possible interaction between the glucose and abscisic acid signaling pathway is discussed . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Glucose effects on the expression of the abscisic acid-inducible Rab16A gene were examined in rice and barley embryos .
[ Sen. 2, subscore: 1.00 ]: Glucose feeding to rice embryos negatively affects the endogenous abscisic acid content and represses the promoter activity of the Rab16A gene .
[ Sen. 3, subscore: 1.00 ]: Glucose repression of the Rab16A gene takes place both at a transcriptional and a post-transcriptional level .
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Score: 3.00 |
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| Title: Comparative functional analysis of three abiotic stress-inducible promoters in transgenic rice .
| | Author: Rai M He C Wu R | | Journal: Transgenic Res Citation: V : P : Year: 2009 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub19357984 Accession (PMID): 19357984 | | Abstract: To identify minimal effective promoters for driving abiotic stress-inducible transgene expression in rice , we selected promoter elements of three stress-responsive genes , viz . rab16A coding for dehydrin , OsABA2 coding for zeaxanthin epoxidase , and a gene coding for a hypothetical protein ( HP1 ) based on the presence of ABA- , salt and drought-responsive cis-acting elements .
These were translationally fused to the gusA reporter gene and introduced into rice to study their effect on heterologous gene expression .
The OsABA2 promoter was found to be the most effective and desirable promoter among the three in terms of driving a low constitutive transgene expression under normal conditions and high induction in response to ABA , salt and drought stress , the highest being a 12-fold induction in response to ABA .
The rab16A and HP1 promoters resulted in high levels of constitutive expression .
While induction of GUS activity was generally two to threefold for all the treatments in roots for both the promoters , induction in leaves was generally insignificant , the exceptions being rab16A in response to continuous salt stress and HP1 in response to water deficit .
It was also observed that the three promoters , in general , resulted in lower constitutive expression , but higher induction in roots as compared to leaves .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: To identify minimal effective promoters for driving abiotic stress-inducible transgene expression in rice , we selected promoter elements of three stress-responsive genes , viz . rab16A coding for dehydrin , OsABA2 coding for zeaxanthin epoxidase , and a gene coding for a hypothetical protein ( HP1 ) based on the presence of ABA- , salt and drought-responsive cis-acting elements .
[ Sen. 4, subscore: 1.00 ]: The rab16A and HP1 promoters resulted in high levels of constitutive expression .
[ Sen. 5, subscore: 1.00 ]: While induction of GUS activity was generally two to threefold for all the treatments in roots for both the promoters , induction in leaves was generally insignificant , the exceptions being rab16A in response to continuous salt stress and HP1 in response to water deficit .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: The rab16B promoter of rice contains two distinct abscisic acid-responsive elements .
| | Author: Ono A Izawa T Chua NH Shimamoto K | | Journal: Plant Physiol .
Citation: V : 112 ( 2 ) P : 483-91 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8883374 Accession (PMID): 8883374 | | Abstract: To localize abscisic acid ( ABA ) -inducible gene expression of rab16 genes , rab16A promoter was linked to the gusA reporter gene encoding beta-glucuronidase and introduced into rice ( Oryza sativa L ) plants .
The activity of rab16A promoter was induced by ABA and osmotic stresses in various it issues of vegetative and floral organs .
In anthers and embryos , rab16A promoter was active in the absence of ABA .
To elucidate cis-elements of the rab16 promoter that confer ABA-inducible expression , variously modified 40-bp fragments ( -264 to -225 ) of the rab16B promoter were fused to a truncated ( -46 bp ) cauliflower mosaic virus 35S minimal promoter , and their activities in protoplasts were analyzed .
The transient assays revealed that the 40-bp fragment consists of two separate ABA-responsive elements , motif 1 ( AGTACGTGGC ) and motif III ( GCCGCGTGGC ) .
Motif I and motif III are both required for ABA induction ; however , each can substitute for the other .
Further analyses of these motifs indicated that motif III has a distinct DNA sequence specificity as an ABA-responsive element from motif I , suggesting that the two motifs interact with different transcription factors in vivo . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: To localize abscisic acid ( ABA ) -inducible gene expression of rab16 genes , rab16A promoter was linked to the gusA reporter gene encoding beta-glucuronidase and introduced into rice ( Oryza sativa L ) plants .
[ Sen. 2, subscore: 1.00 ]: The activity of rab16A promoter was induced by ABA and osmotic stresses in various it issues of vegetative and floral organs .
[ Sen. 3, subscore: 1.00 ]: In anthers and embryos , rab16A promoter was active in the absence of ABA .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
