11 matches found in 8 documents. Search time: 0.036 seconds. |
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Score: 2.00 | Title: Pathogen resistance of transgenic rice plants expressing mitogen-activated protein kinase 1 , MK1 , from Capsicum annuum .
| Author: Lee DE Lee IJ Han O Baik MG Han SS Back K | Journal: Mol .
Cells Citation: V : 17 ( 1 ) P : 81-5 Year: 2004 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub15055532 Accession (PMID): 15055532 | Abstract: Mitogen-activated protein kinase 1 ( MK1 ) from Capsicum annuum was previously cloned and characterized .
MK1 is highly conserved in plants and its amino acid sequence is 92% identical to wound-inducible protein kinase ( WIPK ) from tobacco In C annuum , MK1 is transcriptionally activated by wounding .
In the present work , the MK1 gene was introduced into the rice genome by Agrobacterium-mediated transformation .
Seven independent transgenic rice plants were isolated and characterized .
All seven lines had a single-copy insertion of the MK1 transgene .
MK1 mRNA and protein were detected in the transgenic rice , but not in wild type rice .
In unwounded transgenic rice plants , the level of jasmonic acid was 3-fold higher than in the wild type , whereas in wounded leaves the level of jasmonic acid was the same in transgenic and wild type plants .
Expression of the wound-inducible pathogenesis-related gene PR1a was also higher in transgenic than in wild type plants .
Either overexpression of PR1a or increased PR1b and PR10 expression in transgenic rice plants may confer increased resistance to rice blast | Matching Sentences: [ Sen. 8, subscore: 1.00 ]: Expression of the wound-inducible pathogenesis-related gene PR1a was also higher in transgenic than in wild type plants . [ Sen. 9, subscore: 1.00 ]: Either overexpression of PR1a or increased PR1b and PR10 expression in transgenic rice plants may confer increased resistance to rice blast
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Score: 2.00 | Title: Expression of defense genes and antioxidant defense responses in rice resistance to neck blast at the preliminary heading stage and full heading stage .
| Author: Hao Z Wang L Huang F Tao R | Journal: Plant Physiol Biochem Citation: V : 57 P : 222-30 Year: 2012 Type: In-Data-Review | Literature: oryza Field: abstract Doc ID: pub22738867 Accession (PMID): 22738867 | Abstract: The rice variety Xiushui227 is resistant to neck blast at three crucial panicle stages ( the booting stage , the preliminary heading stage ( PHS ) , and the full heading stage ( FHS ) ) that controlling neck blast The other rice variety Liangyou6326 is susceptible to neck blast at these three panicle stages .
These two varieties were used to analyze the expression patterns of defense genes and antioxidant defense responses at the PHS and FHS , after inoculating the necks in vitro with Magnaporthe oryzae , respectively .
All defense genes ( pathogenesis-related genes PR1a , Gns1 ( 1 , 3 ; 1 , 4-beta-glucanase ) , Cht-1 ( chitinase ) , PR4 , PR5 , and PR10a , secondary metabolite pathway genes PAL ( phenylalanine ammonia-lyase ) , CHS ( chalcone synthase ) , and LOX ( lipoxygenase ) , and oxidative stress-related protein genes POX22 . 3 ( peroxidase ) , and PPO ( polyphenol oxidase ) ) used in this experiment except Cht-1 , CHS and PPO could mainly play important roles in the resistance to neck blast at the PHS in Xiushui227 , and CHS and PPO may primarily participate in fighting back against to neck blast at the FHS .
Conversely , PR1a , Cht-1 , PR4 , PR10a , CHS , LOX-RLL , and PPO could chiefly play participate in defending Liangyou6326 against neck blast at the FHS , and PR5 , PAL , and POX22 . 3 may be primarily involved in fighting back against to neck blast at the PHS .
Furthermore , the antioxidant enzymes superoxide dismutase , peroxidase , and catalase may mainly participate in defending Xiushui227 against neck blast at the PHS and defending Liangyou6326 against neck blast at the FHS , respectively .
Therefore oxidative damage is less at the PHS in Xiushui227 and at the FHS in Liangyou6326 , respectively .
| Matching Sentences: [ Sen. 3, subscore: 1.00 ]: All defense genes ( pathogenesis-related genes PR1a , Gns1 ( 1 , 3 ; 1 , 4-beta-glucanase ) , Cht-1 ( chitinase ) , PR4 , PR5 , and PR10a , secondary metabolite pathway genes PAL ( phenylalanine ammonia-lyase ) , CHS ( chalcone synthase ) , and LOX ( lipoxygenase ) , and oxidative stress-related protein genes POX22 . 3 ( peroxidase ) , and PPO ( polyphenol oxidase ) ) used in this experiment except Cht-1 , CHS and PPO could mainly play important roles in the resistance to neck blast at the PHS in Xiushui227 , and CHS and PPO may primarily participate in fighting back against to neck blast at the FHS . [ Sen. 4, subscore: 1.00 ]: Conversely , PR1a , Cht-1 , PR4 , PR10a , CHS , LOX-RLL , and PPO could chiefly play participate in defending Liangyou6326 against neck blast at the FHS , and PR5 , PAL , and POX22 . 3 may be primarily involved in fighting back against to neck blast at the PHS .
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Score: 2.00 | Title: Abnormal cell divisions in leaf primordia caused by the expression of the rice homeobox gene OSH1 lead to altered morphology of leaves in transgenic tobacco | Author: Sato Y Tamaoki M Murakami T Yamamoto N Kano-Murakami Y Matsuoka M | Journal: Mol . Gen . Genet . Citation: V : 251 ( 1 ) P : 13-22 Year: 1996 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub8628242 Accession (PMID): 8628242 | Abstract: Transgenic tobacco plants were generated carrying a rice homeobox gene , OSH1 , controlled by the promoter of a gene encoding a tobacco pathogenesis-related protein ( PR1a ) .
These lines were morphologically abnormal , with wrinkled and/or lobed leaves .
Histological analysis of shoot apex primordia indicates arrest of lateral leaf blade expansion , often resulting in asymmetric and anisotrophic growth of leaf blades .
Other notable abnormalities included abnormal or arrested development of leaf lateral veins .
Interestingly , OHS1 expression was undetectable in mature leaves with the aberrant morphological features .
Thus , OSH1 expression in mature leaves is not necessary for abnormal leaf development .
Northern blot and in situ hybridization analyses indicate that PR1a-OSH1 is expressed only in the shoot apical meristem and in very young leaf primordia .
Therefore , the aberrant morphological features are an indirect consequence of ectopic OSH1 gene expression .
The only abnormality observed in it issues expressing the transgene was periclinal ( rather than anticlinal ) division in mesophyll cells during leaf blade initiation .
This generates thicker leaf blades and disrupts the mesophyll cell layers , from which vascular it issues differentiate .
The OSH1 product appears to affect the mechanism controlling the orientation of the plane of cell division , resulting in abnormal periclinal division of mesophyll cell , which in turn results in the gross morphological abnormalities observed in the transgenic lines . | Matching Sentences: [ Sen. 1, subscore: 1.00 ]: Transgenic tobacco plants were generated carrying a rice homeobox gene , OSH1 , controlled by the promoter of a gene encoding a tobacco pathogenesis-related protein ( PR1a ) . [ Sen. 7, subscore: 1.00 ]: Northern blot and in situ hybridization analyses indicate that PR1a-OSH1 is expressed only in the shoot apical meristem and in very young leaf primordia .
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Score: 1.00 | Title: Inducible overexpression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level , PR gene expression , and host resistance to fungal infection .
| Author: Mei C Qi M Sheng G Yang Y | Journal: Mol .
Plant Microbe Interact .
Citation: V : 19 ( 10 ) P : 1127-37 Year: 2006 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub17022177 Accession (PMID): 17022177 | Abstract: Many studies in dicotyledonous plants have shown that jasmonates , including jasmonic acid ( JA ) and methyl jasmonate , are important signal molecules involved in induced resistance to pathogen infection and insect herbivory .
However , very little genetic and molecular evidence is available to demonstrate their role in host defense response of rice and other economically important monocot plants .
In this study , we have shown that exogenous application of JA was able to activate defense gene expression and local induced resistance in rice seedlings against the rice blast fungus ( Magnaporthe grisea ) .
Furthermore , we have characterized a pathogen-inducible rice OsAOS2 gene ( which encodes allene oxide synthase , a key enzyme in the JA biosynthetic pathway ) and examined the role of endogenous JA in rice defense response through transgenic manipulation of the JA biosynthesis .
Sequence analysis indicated that OsAOS2 contains four common domains of the cytochrome P450 enzyme , but does not have the signal peptide for chloroplast targeting .
The basal level of OsAOS2 expression is very low in leaves but relatively high in the sheath , culm , and flower of rice plants .
Interestingly , the expression of OsAOS2 in rice leaves can be induced significantly upon M grisea infection .
Transgenic rice lines carrying the OsAOS2 transgene under the control of a strong , pathogen-inducible PBZ1 promoter accumulated abundant OsAOS2 transcripts and higher levels of JA , especially after the pathogen infection .
These transgenic lines also exhibited enhanced activation of pathogenesis-related ( PR ) genes such as PR1a , PR3 , and PR5 and increased resistance to M grisea infection .
Our results suggest that JA plays a significant role in PR gene induction and blast resistance in rice plants . | Matching Sentences: [ Sen. 9, subscore: 1.00 ]: These transgenic lines also exhibited enhanced activation of pathogenesis-related ( PR ) genes such as PR1a , PR3 , and PR5 and increased resistance to M grisea infection .
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Score: 1.00 | Title: Opposite functions of a rice mitogen-activated protein kinase during the process of resistance against Xanthomonas oryzae .
| Author: Shen X Yuan B Liu H Li X Xu C Wang S | Journal: Plant J Citation: V : 64 P : 86-99 Year: 2010 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub20663089 Accession (PMID): 20663089 | Abstract: The pathogen-induced plant defense signaling network consists of multiple components , although only some of them are characterized .
Most of the known components function either as activators or repressors in host-pathogen interactions .
Here we report that a mitogen-activated protein kinase , OsMPK6 , functions both as an activator and a repressor in rice resistance against Xanthomonas oryzae pv . oryzae ( Xoo ) , the causal organism of bacterial blight disease .
Activation of OsMPK6 resulted in the formation of lesion mimics and local resistance to Xoo , accompanied by the accumulation of salicylic acid ( SA ) and jasmonic acid ( JA ) , and the induced expression of SA and JA-signaling genes .
Nuclear localization of OsMPK6 was essential for local resistance , suggesting that modulating the expression of defense-responsive genes through transcription regulators may be the primary mechanism of OsMPK6-mediated local resistance .
The knock-out of OsMPK6 resulted in enhanced Xoo resistance , increased accumulation of SA and enhanced resistance to X oryzae pv . oryzicola , the causal organism of bacterial streak disease , in systemic it issues .
Xoo infection induced the expression of PR1a , the marker gene of systemic acquired resistance ( SAR ) , in systemic health it issues of OsMPK6-knock-out plants .
These results suggest that OsMPK6 negatively regulates SAR .
Thus OsMPK6 is a two-faced player in the rice-Xoo interaction .
| Matching Sentences: [ Sen. 7, subscore: 1.00 ]: Xoo infection induced the expression of PR1a , the marker gene of systemic acquired resistance ( SAR ) , in systemic health it issues of OsMPK6-knock-out plants .
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Score: 1.00 | Title: Expression of defense genes and activities of antioxidant enzymes in rice resistance to rice stripe virus and small brown planthopper .
| Author: Hao Z Wang L He Y Liang J Tao R | Journal: Plant Physiol Biochem Citation: V : 49 P : 744-51 Year: 2011 Type: In-Process | Literature: oryza Field: abstract Doc ID: pub21300551 Accession (PMID): 21300551 | Abstract: The rice variety Tai06-1 is resistant to rice stripe disease and Xiushui63 is a highly susceptible rice variety to this disease .
These two varieties were used to analyze the expression patterns of defense genes and antioxidant defense responses at the seedling stage , upon feeding with viruliferous small brown planthopper ( SBPH ) and nonviruliferous SBPH , respectively .
The expression levels of CP ( coat protein ) gene of rice stripe virus ( RSV ) were higher upon feeding with viruliferous SBPH in Xiushui63 than in Tai06-1 throughout most of the experimental period , suggesting that RSV replicaiton is disturbed in Tai06-1 but not in Xiushui63 , therefore , the resistance to RSV is higher in Tai06-1 than in Xiuhsui63 .
We found that defense genes PR1a ( pathogenesis-related class 1a ) , PAL ( phenylalanine ammonia-lyase ) , and CHS ( chalcone synthase ) may play roles in the defense responses to both RSV and SBPH in Tai06-1 , and PR4 and PR10a may only participate in defending against SBPH attack but not against RSV infection in Tai06-1 .
Our data reveal that Gns1 ( 1 , 3 ; 1 , 4-beta-glucanase ) may participate in the defense responses to both RSV and SBPH in Xiushui63 but not in Tai06-1 , and LOX ( lipoxygenase ) may only participate in defending against to SBPH in both Tai06-1 and Xiushui63 .
The antioxidant enzymes superoxide dismutase , peroxidase , catalase , hydrogen peroxide , and malondialdehyde coordinately participate in the resistance to RSV in Tai06-1 , and that oxidative damage is less in Tai06-1 than in Xiushui63 .
| Matching Sentences: [ Sen. 4, subscore: 1.00 ]: We found that defense genes PR1a ( pathogenesis-related class 1a ) , PAL ( phenylalanine ammonia-lyase ) , and CHS ( chalcone synthase ) may play roles in the defense responses to both RSV and SBPH in Tai06-1 , and PR4 and PR10a may only participate in defending against SBPH attack but not against RSV infection in Tai06-1 .
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Score: 1.00 | Title: Phytochromes regulate SA and JA signaling pathways in rice and are required for developmentally controlled resistance to Magnaporthe grisea .
| Author: Xie XZ Xue YJ Zhou JJ Zhang B Chang H Takano M | Journal: Mol Plant Citation: V : 4 P : 688-96 Year: 2011 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub21357645 Accession (PMID): 21357645 | Abstract: Old leaves of wild-type rice plants ( Oryza sativa L cv .
Nipponbare ) are more resistant to blast fungus ( Magnaporthe grisea ) than new leaves .
In contrast , both old and new leaves of the rice phytochrome triple mutant ( phyAphyBphyC ) are susceptible to blast fungus .
We demonstrate that pathogenesis-related class 1 ( PR1 ) proteins are rapidly and strongly induced during M grisea infection and following exogenous jasmonate ( JA ) or salicylic acid ( SA ) exposure in the old leaves , but not in the new leaves of the wild-type .
In contrast , the accumulation of PR1 proteins was significantly attenuated in old and new leaves of the phyAphyBphyC mutant .
These results suggest that phytochromes are required for the induction of PR1 proteins in rice .
Basal transcription levels of PR1a and PR1b were substantially higher in the wild-type as compared to the phyAphyBphyC mutant , suggesting that phytochromes also are required for basal expression of PR1 genes .
Moreover , the transcript levels of genes known to function in SA or JA-dependent defense pathways were regulated by leaf age and functional phytochromes .
Taken together , our findings demonstrate that phytochromes are required in rice for age-related resistance to M grisea and may indirectly increase PR1 gene expression by regulating SA and JA-dependent defense pathways .
| Matching Sentences: [ Sen. 7, subscore: 1.00 ]: Basal transcription levels of PR1a and PR1b were substantially higher in the wild-type as compared to the phyAphyBphyC mutant , suggesting that phytochromes also are required for basal expression of PR1 genes .
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Score: 1.00 | Title: The salicylic acid-inducible alternative oxidase gene aox1 and genes encoding pathogenesis-related proteins share regions of sequence similarity in their promoters .
| Author: Rhoads DM McIntosh L | Journal: Plant Mol . Biol . Citation: V : 21 ( 4 ) P : 615-24 Year: 1993 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub8448361 Accession (PMID): 8448361 | Abstract: We have isolated and characterized a genomic clone , lambda AOSG11 , corresponding to aox1 , which encodes the 42 kDa alternative oxidase precursor protein of Sauromatum guttatum Schott .
The sequence of lambda AOSG11 revealed that aox1 consists of four exons separated by three short introns .
Exon three contains the region of aox1 that ( 1 ) is highly conserved in the corresponding genes of potato , rice , and yeast , and ( 2 ) encodes a region of the deduced protein that is predicted to form two transmembrane alpha-helices .
Southern blot analysis of restriction endonuclease-digested genomic DNA , indicated that aox1 is a single , nuclear-encoded gene in S guttatum .
We have determined the transcriptional start site of aox1 using nuclease protection and primer extension experiments .
Comparison of the putative promoter region of aox1 to promoters of PR1a and GRP8 revealed some sequence similarity .
| Matching Sentences: [ Sen. 6, subscore: 1.00 ]: Comparison of the putative promoter region of aox1 to promoters of PR1a and GRP8 revealed some sequence similarity .
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