| 457 matches found in 226 documents. Search time: 0.753 seconds. |
|---|
|
Score: 14.00 |
|---|
| Title: Detection of QTLs with additive effects and additive-by-environment interaction effects on panicle number in rice ( Oryza sativa L ) with single-segment substitution lines .
| | Author: Liu G Zhang Z Zhu H Zhao F Ding X Zeng R Li W Zhang G | | Journal: Theor Appl Genet Citation: V : 116 P : 923-31 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18274724 Accession (PMID): 18274724 | | Abstract: A novel population consisting of 35 single-segment substitution lines ( SSSLs ) originating from crosses between the recipient parent , Hua-jing-xian 74 ( HJX74 ) , and 17 donor parents was evaluated in six cropping season environments to reveal the genetic basis of genetic main effect ( G ) and genotype-by-environment interaction effect ( GE ) for panicle number ( PN ) in rice .
Subsets of lines were grown in up to six environments .
An indirect analysis method was applied , in which the total genetic effect was first partitioned into G and GE by using the mixed linear-model approach , and then QTL ( quantitative trait locus ) analyses on these effects were conducted separately .
At least 18 QTLs for PN in rice were detected and identified on 9 of 12 rice chromosomes .
A single QTL effect ( a + ae ) ranging from -1 . 5 to 1 . 2 was divided into two components , additive effect ( a ) and additive x environment interaction effect ( ae ) .
A total number of 9 and 16 QTLs were identified with a ranging from -0 . 4 to 0 . 6 and ae ranging from -1 . 0 to 0 . 6 , respectively , the former being stable but the latter unstable across environments .
Three types of QTLs were suggested according to their effects expressed .
Two QTLs ( Pn-1b and Pn-6d ) expressed stably across environments due to the association with only a , nine QTLs ( Pn-1a , Pn-3c , Pn-3d , Pn-4 , Pn-6a , Pn-6b , Pn-8 , Pn-9 and Pn-12 ) with only ae were unstable , and the remaining seven of QTLs were identified with both a and ae , which also were unstable across environments .
This is the first report on the detection of QE ( QTL-by-environment interaction effect ) of QTLs with SSSLs .
Our results illustrate the efficiency of characterizing QTLs and analyzing action of QTLs through SSSLs , and further demonstrate that QE is an important property of many QTLs .
Information provided in this paper could be used in the application of marker-assisted selection to manipulate PN in rice .
| Matching Sentences:
[ Sen. 8, subscore: 11.00 ]: Two QTLs ( Pn-1b and Pn-6d ) expressed stably across environments due to the association with only a , nine QTLs ( Pn-1a , Pn-3c , Pn-3d , Pn-4 , Pn-6a , Pn-6b , Pn-8 , Pn-9 and Pn-12 ) with only ae were unstable , and the remaining seven of QTLs were identified with both a and ae , which also were unstable across environments .
[ Sen. 1, subscore: 1.00 ]: A novel population consisting of 35 single-segment substitution lines ( SSSLs ) originating from crosses between the recipient parent , Hua-jing-xian 74 ( HJX74 ) , and 17 donor parents was evaluated in six cropping season environments to reveal the genetic basis of genetic main effect ( G ) and genotype-by-environment interaction effect ( GE ) for panicle number ( PN ) in rice .
[ Sen. 4, subscore: 1.00 ]: At least 18 QTLs for PN in rice were detected and identified on 9 of 12 rice chromosomes .
[ Sen. 11, subscore: 1.00 ]: Information provided in this paper could be used in the application of marker-assisted selection to manipulate PN in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 |
|---|
| Title: Purification and characterization of beta-N-acetylhexosaminidase from rice seeds .
| | Author: Jin YL Jo YY Kim KY Shim JH Kim YW Park RD .
| | Journal: J Biochem . Mol . Biol . Citation: V : 35 ( 3 ) P : 313-9 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12297015 Accession (PMID): 12297015 | | Abstract: N-Acetyl-beta-D-hexosaminidase ( beta-HexNAcase ) ( EC 3 . 2 . 1 . 52 ) was purified from rice seeds ( Oryza sativa L var .
Dongjin ) using ammonium sulfate ( 80% ) precipitation , Sephadex G-150 , CM-Sephadex , and DEAE-Sephadex chromatography , sequentially .
The activities were separated into 7 fractions ( Fsub1 ; - F7sub7 ) by CM-Sephadex chromatography .
Among them , F6 was further purified to homogeneity with a 13 . 0% yield and 123 . 3 purification-fold .
The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration .
The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase .
The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 .
However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside .
The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively .
The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site .
The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively .
The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity .
Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase .
[ Sen. 7, subscore: 2.00 ]: The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 .
[ Sen. 8, subscore: 2.00 ]: However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside .
[ Sen. 10, subscore: 2.00 ]: The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site .
[ Sen. 9, subscore: 1.00 ]: The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively .
[ Sen. 11, subscore: 1.00 ]: The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively .
[ Sen. 12, subscore: 1.00 ]: The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 9.00 |
|---|
| Title: A circadian rhythm set by dusk determines the expression of FT homologs and the short-day photoperiodic flowering response in Pharbitis .
| | Author: Hayama R Agashe B Luley E King R Coupland G | | Journal: Plant Cell Citation: V : 19 P : 2988-3000 Year: 2007 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub17965272 Accession (PMID): 17965272 | | Abstract: Seasonal control of flowering through responsiveness to daylength shows extreme variation .
Different species flower in response to long days or short days ( SDs ) , and this difference evolved several times .
The molecular mechanisms conferring these responses have been compared in detail only in Arabidopsis thaliana and rice ( Oryza sativa ) and suggest that a conserved pathway confers daylength responses through regulation of FLOWERING LOCUS T ( FT ) transcription by CONSTANS ( CO ) .
We studied Pharbitis ( Ipomoea nil ; formerly , Pharbitis nil ) , a widely used SD model species and a member of the Convolvulaceae , and showed using transgenic plants together with detailed expression analysis that two putative orthologs of FT ( Pn FT1 and Pn FT2 ) promote flowering specifically under SDs .
These genes are expressed only under SDs , and light flashes given during the night reduce their expression and prevent flowering .
We demonstrate that in Pharbitis a circadian rhythm set by the light-to-dark transition at dusk regulates Pn FT expression , which rises only when the night is longer than 11 h .
Furthermore , Pharbitis accessions that differ in their critical night-length responses express Pn FT at different times after dusk , demonstrating that natural genetic variation influencing the clock regulating Pn FT expression alters the flowering response .
In these assays , Pn FT mRNA abundance was not related to Pn CO expression , suggesting that Pn FT may be regulated by a different transcription factor in Pharbitis .
We conclude that SD response in Pharbitis is controlled by a dedicated light sensitive clock , set by dusk , that activates Pn FT transcription in darkness , a different mechanism for measuring daylength than described for Arabidopsis and rice .
| Matching Sentences:
[ Sen. 8, subscore: 3.00 ]: In these assays , Pn FT mRNA abundance was not related to Pn CO expression , suggesting that Pn FT may be regulated by a different transcription factor in Pharbitis .
[ Sen. 4, subscore: 2.00 ]: We studied Pharbitis ( Ipomoea nil ; formerly , Pharbitis nil ) , a widely used SD model species and a member of the Convolvulaceae , and showed using transgenic plants together with detailed expression analysis that two putative orthologs of FT ( Pn FT1 and Pn FT2 ) promote flowering specifically under SDs .
[ Sen. 7, subscore: 2.00 ]: Furthermore , Pharbitis accessions that differ in their critical night-length responses express Pn FT at different times after dusk , demonstrating that natural genetic variation influencing the clock regulating Pn FT expression alters the flowering response .
[ Sen. 6, subscore: 1.00 ]: We demonstrate that in Pharbitis a circadian rhythm set by the light-to-dark transition at dusk regulates Pn FT expression , which rises only when the night is longer than 11 h .
[ Sen. 9, subscore: 1.00 ]: We conclude that SD response in Pharbitis is controlled by a dedicated light sensitive clock , set by dusk , that activates Pn FT transcription in darkness , a different mechanism for measuring daylength than described for Arabidopsis and rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
|---|
| Title: [ Analysis of additive and AE interaction effects of QTLs controlling plant height , heading date and panicle number in rice ( Oryza sativa L ) ] | | Author: Yuan AP Cao LY Zhuang JY Li RZ Zheng KL Zhu J Cheng SH .
| | Journal: Yi Chuan Xue Bao Citation: V : 30 ( 10 ) P : 899-906 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14669505 Accession (PMID): 14669505 | | Abstract: Plant height ( PH ) , heading date ( HD ) and productive panicle number ( PN ) are important agronomic trait in rice .
Appropriate plant height , heading date and panicle number are prerequisites for the desired high and stable yield level in rice breeding programs .
A recombinant inbred line ( RIL ) population consisting of 304 individuals was derived from a cross between indica varieties Zhong156 and Gumei2 , from which a linkage map consisting of 168 RFLP , SSLP , RAPD and RGA markers that distribute on all the 12 rice chromosomes was constructed , and covers 1447 . 9 cM of the rice genome .
The parents and 304 F9 lines were grown in the paddy field in China National Rice Research Institute ( CNRRI ) , Hangzhou , China in 2001 .
The experiments were carried out in two seasons followed a randomized complete block design .
QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well .
A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction .
Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction .
These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects .
In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively .
Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only .
Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction .
No epistatic QE interactions was detected .
In addition , conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well .
[ Sen. 1, subscore: 1.00 ]: Plant height ( PH ) , heading date ( HD ) and productive panicle number ( PN ) are important agronomic trait in rice .
[ Sen. 8, subscore: 1.00 ]: Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction .
[ Sen. 9, subscore: 1.00 ]: These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects .
[ Sen. 10, subscore: 1.00 ]: In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively .
[ Sen. 12, subscore: 1.00 ]: Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction .
[ Sen. 14, subscore: 1.00 ]: In addition , conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
|---|
| Title: Nucleic acid binding activity of pns6 encoded by genome segment 6 of rice ragged stunt oryzavirus .
| | Author: Shao CG L HJ Wu JH Gong ZX .
| | Journal: Acta Biochim . Biophys . Sin . ( Shanghai ) Citation: V : 36 ( 7 ) P : 457-66 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15248020 Accession (PMID): 15248020 | | Abstract: The ORF of genome segment 6 ( S6 ) of rice ragged stunt oryzavirus ( RRSV ) Philippines isolate was cloned and sequenced based on the S6 sequence of the Thailand isolate .
Pns6 , the 71 kD product of S6 expressed in E coli , was demonstrated to be a viral non-structural protein of RRSV by Western blotting .
The gel mobility shift assays showed that Pns6 had nucleic acid binding activity .
Pns6 could interact with single and double-stranded forms of DNA and RNA , showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA , as demonstrated by both competition and displacement assays .
The binding of Pns6 to nucleic acids is strong and sequence non-specific .
By using five truncated derivatives of Pns6 , it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain .
Subcellular fractionation of leaf it issues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction .
The possible role of RRSV Pns6 in virus replication and assembly is discussed . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: By using five truncated derivatives of Pns6 , it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain .
[ Sen. 2, subscore: 1.00 ]: Pns6 , the 71 kD product of S6 expressed in E coli , was demonstrated to be a viral non-structural protein of RRSV by Western blotting .
[ Sen. 3, subscore: 1.00 ]: The gel mobility shift assays showed that Pns6 had nucleic acid binding activity .
[ Sen. 4, subscore: 1.00 ]: Pns6 could interact with single and double-stranded forms of DNA and RNA , showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA , as demonstrated by both competition and displacement assays .
[ Sen. 5, subscore: 1.00 ]: The binding of Pns6 to nucleic acids is strong and sequence non-specific .
[ Sen. 7, subscore: 1.00 ]: Subcellular fractionation of leaf it issues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction .
[ Sen. 8, subscore: 1.00 ]: The possible role of RRSV Pns6 in virus replication and assembly is discussed .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
|---|
| Title: Treatment of p-nitrophenol in an adsorbent-supplemented sequencing batch reactor .
| | Author: Loo YM Lim PE Seng CE | | Journal: Environ Technol Citation: V : 31 P : 479-87 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20480823 Accession (PMID): 20480823 | | Abstract: The objective of this research was to evaluate the treatment ofp-nitrophenol ( PNP ) as a sole organic carbon source using a sequencing batch reactor ( SBR ) with the addition of adsorbent .
Two types of adsorbents , namely powdered activated carbon ( PAC ) and pyrolysed rice husk ( PRH ) were used in this study .
Two identical SBRs , each with a working volume of 10 L , were operated with fill , react , settle , draw and idle periods in the ratio of 2 : 8 : 1 : 0 . 75 : 0 . 25 for a cycle time of 12 h .
The results showed that , without the addition of adsorbent , increasing the influent PNP concentration to 200 mg/L resulted in the deterioration of chemical oxygen demand ( COD ) removal efficiency and PNP removal efficiency in the SBRs .
Improvement in the performance of the SBR was observed with the addition of PAC .
When the dosage of 1 . 0 g PAC/cycle was applied , COD removal of 95% and almost complete removal of PNP were achieved at the influent PNP concentration of 300 mg/L The kinetic study showed that the rates of COD and PNP removal can be described by the first-order kinetics .
The enhancement of performance in the PAC-supplemented SBR was postulated to be due to the initial adsorption of PNP by the freshly added and the bioregenerated PAC , thus reducing the inhibition on the microorganisms .
The PRH was found to be ineffective because of its relatively low adsorption capacity for PNP , compared with that of PAC .
| Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: When the dosage of 1 . 0 g PAC/cycle was applied , COD removal of 95% and almost complete removal of PNP were achieved at the influent PNP concentration of 300 mg/L The kinetic study showed that the rates of COD and PNP removal can be described by the first-order kinetics .
[ Sen. 4, subscore: 2.00 ]: The results showed that , without the addition of adsorbent , increasing the influent PNP concentration to 200 mg/L resulted in the deterioration of chemical oxygen demand ( COD ) removal efficiency and PNP removal efficiency in the SBRs .
[ Sen. 1, subscore: 1.00 ]: The objective of this research was to evaluate the treatment ofp-nitrophenol ( PNP ) as a sole organic carbon source using a sequencing batch reactor ( SBR ) with the addition of adsorbent .
[ Sen. 7, subscore: 1.00 ]: The enhancement of performance in the PAC-supplemented SBR was postulated to be due to the initial adsorption of PNP by the freshly added and the bioregenerated PAC , thus reducing the inhibition on the microorganisms .
[ Sen. 8, subscore: 1.00 ]: The PRH was found to be ineffective because of its relatively low adsorption capacity for PNP , compared with that of PAC .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
|---|
| Title: Rice ragged stunt virus segment S6-encoded nonstructural protein Pns6 complements cell-to-cell movement of Tobacco mosaic virus-based chimeric virus .
| | Author: Wu Z Wu J Adkins S Xie L Li W | | Journal: Virus Res Citation: V : 152 P : 176-9 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20541571 Accession (PMID): 20541571 | | Abstract: The protein ( s ) that support intercellular movement of Rice ragged stunt virus ( RRSV ) have not yet been identified .
In this study , the role of three nonstructural proteins Pns6 , Pns7 and Pns10 in cell-to-cell movement were determined with a movement-deficient Tobacco mosaic virus ( TMV ) vector .
The results showed that only the Pns6 could complement the cell-to-cell movement of the movement-deficient TMV in Nicotiana tabacum Xanthi nc and N benthamiana plants , and both N and C-terminal 50 amino acids of Pns6 were essential for the cell-to-cell movement .
Transient expression in epidermal cells from N benthamiana showed that the Pns6-eGFP fusion protein was present predominantly along the cell wall as well as a few punctate sites perhaps indicating plasmodesmata .
Taken together with previous finding that the Pns6 has nucleic acid-binding activity ( Shao et al , 2004 ) , the possible role of Pns6 in cell-to-cell movement of RRSV were discussed .
| Matching Sentences:
[ Sen. 2, subscore: 3.00 ]: In this study , the role of three nonstructural proteins Pns6 , Pns7 and Pns10 in cell-to-cell movement were determined with a movement-deficient Tobacco mosaic virus ( TMV ) vector .
[ Sen. 3, subscore: 2.00 ]: The results showed that only the Pns6 could complement the cell-to-cell movement of the movement-deficient TMV in Nicotiana tabacum Xanthi nc and N benthamiana plants , and both N and C-terminal 50 amino acids of Pns6 were essential for the cell-to-cell movement .
[ Sen. 5, subscore: 2.00 ]: Taken together with previous finding that the Pns6 has nucleic acid-binding activity ( Shao et al , 2004 ) , the possible role of Pns6 in cell-to-cell movement of RRSV were discussed .
[ Sen. 4, subscore: 1.00 ]: Transient expression in epidermal cells from N benthamiana showed that the Pns6-eGFP fusion protein was present predominantly along the cell wall as well as a few punctate sites perhaps indicating plasmodesmata .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
|---|
| Title: Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure .
| | Author: Akita F Miyazaki N Hibino H Shimizu T Higashiura A Uehara-Ichiki T Sasaya T Tsukihara T Nakagawa A Iwasaki K Omura T | | Journal: J Gen Virol Citation: V : 92 P : 2214-21 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21613445 Accession (PMID): 21613445 | | Abstract: The non-structural Pns9 protein of rice gall dwarf virus ( RGDV ) accumulates in viroplasm inclusions , which are structures that appear to play an important role in viral morphogenesis and are commonly found in host cells infected by viruses in the family Reoviridae .
Immunofluorescence and immunoelectron microscopy of RGDV-infected vector cells in monolayers , using antibodies against Pns9 of RGDV and expression of Pns9 in Spodoptera frugiperda cells , demonstrated that Pns9 is the minimal viral factor necessary for formation of viroplasm inclusion during infection by RGDV .
When Pns9 in solution was observed under a conventional electron microscope , it appeared as ring-like aggregates of approximately 100 A in diameter .
Cryo-electron microscopic analysis of these aggregates revealed cylinders of octameric Pns9 , whose dimensions were similar to those observed under the conventional electron microscope .
Octamerization of Pns9 in solution was confirmed by the results of size-exclusion chromatography .
Among proteins of viruses that belong to the family Reoviridae whose three-dimensional structures are available , a matrix protein of the viroplasm of rotavirus , NSP2 , forms similar octamers , an observation that suggests similar roles for Pns9 and NSP2 in morphogenesis in animal-infecting and in plant-infecting reoviruses .
| Matching Sentences:
[ Sen. 2, subscore: 3.00 ]: Immunofluorescence and immunoelectron microscopy of RGDV-infected vector cells in monolayers , using antibodies against Pns9 of RGDV and expression of Pns9 in Spodoptera frugiperda cells , demonstrated that Pns9 is the minimal viral factor necessary for formation of viroplasm inclusion during infection by RGDV .
[ Sen. 1, subscore: 1.00 ]: The non-structural Pns9 protein of rice gall dwarf virus ( RGDV ) accumulates in viroplasm inclusions , which are structures that appear to play an important role in viral morphogenesis and are commonly found in host cells infected by viruses in the family Reoviridae .
[ Sen. 3, subscore: 1.00 ]: When Pns9 in solution was observed under a conventional electron microscope , it appeared as ring-like aggregates of approximately 100 A in diameter .
[ Sen. 4, subscore: 1.00 ]: Cryo-electron microscopic analysis of these aggregates revealed cylinders of octameric Pns9 , whose dimensions were similar to those observed under the conventional electron microscope .
[ Sen. 5, subscore: 1.00 ]: Octamerization of Pns9 in solution was confirmed by the results of size-exclusion chromatography .
[ Sen. 6, subscore: 1.00 ]: Among proteins of viruses that belong to the family Reoviridae whose three-dimensional structures are available , a matrix protein of the viroplasm of rotavirus , NSP2 , forms similar octamers , an observation that suggests similar roles for Pns9 and NSP2 in morphogenesis in animal-infecting and in plant-infecting reoviruses .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
|---|
| Title: Molecular marker dissection of rice ( Oryza sativa L ) plant architecture under temperate and tropical climates .
| | Author: Kobayashi S Fukuta Y Sato T Osaki M Khush GS .
| | Journal: Theor . Appl . Genet . Citation: V : 107 ( 8 ) P : 1350-6 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12920520 Accession (PMID): 12920520 | | Abstract: Rice ( Oryza sativa L ) plants develop vertically with shoot elongation and horizontally with tillering .
The purpose of this study was to identify and characterize genomic regions influencing the rice plant architecture by quantitative trait locus ( QTL ) analysis for the component traits : culm length ( CL ) , panicle length ( PnL ) , panicle number ( PnN ) and tiller number ( TN ) .
For this QTL analysis , 191 recombinant inbred lines ( F ( 7 ) ) derived from a cross of Milyang 23 ( M23 ) and Akihikari ( AK ) were grown in 1995 , 1996 and 1997 ( May-Oct ) in Joetsu , Japan ( temperate climate ) , and in the 2000 dry season ( Jan-Apr ) , the 2000 wet season ( Jun-Oct ) and the 2001 dry season in Los Baos , The Philippines ( tropical climate ) .
Results showed that rice plant architecture was influenced by 19 genomic regions categorized into five groups .
In Group I , two regions ( on chrs .
6 and 11 ) affected shoot elongation ( CL and PnL ) and tillering ( PnN and TN ) in opposite directions more significantly in Los Baos than in Joetsu .
In Group II , two regions ( chrs .
3 and 12 ) affected shoot elongation , whereas in Group III , five regions [ chrs .
1 ( two ) , 2 , 3 and 9 ] affected only culm length ( CL ) .
Expressions of four regions of Group III were influenced by either tropical or temperate environments .
In Group IV , seven regions ( chrs .
1 , 2 , 4 , 5 , 6 , 8 and 9 ) controlled panicle development ( PnN or PnL ) , and in Group V , three regions ( chrs .
1 , 2 and 3 ) regulated tillering ( PnN or TN ) .
Characterizing these 19 genomic regions provided a detailed analysis of rice plant architecture with emphasis on the multiple effect and environmental responsive regions . | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: The purpose of this study was to identify and characterize genomic regions influencing the rice plant architecture by quantitative trait locus ( QTL ) analysis for the component traits : culm length ( CL ) , panicle length ( PnL ) , panicle number ( PnN ) and tiller number ( TN ) .
[ Sen. 6, subscore: 2.00 ]: 6 and 11 ) affected shoot elongation ( CL and PnL ) and tillering ( PnN and TN ) in opposite directions more significantly in Los Baos than in Joetsu .
[ Sen. 12, subscore: 2.00 ]: 1 , 2 , 4 , 5 , 6 , 8 and 9 ) controlled panicle development ( PnN or PnL ) , and in Group V , three regions ( chrs .
[ Sen. 13, subscore: 1.00 ]: 1 , 2 and 3 ) regulated tillering ( PnN or TN ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
|---|
| Title: Silencing by RNAi of the gene for Pns12 , a viroplasm matrix protein of Rice dwarf virus , results in strong resistance of transgenic rice plants to the virus .
| | Author: Shimizu T Yoshii M Wei T Hirochika H Omura T | | Journal: Plant Biotechnol J Citation: V : 7 P : 24-32 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18761654 Accession (PMID): 18761654 | | Abstract: The non-structural protein Pns12 of Rice dwarf virus is one of the early proteins expressed in cultured insect cells , and it is one of 12 proteins that initiate the formation of the viroplasm , the putative site of viral replication .
Pns4 is also a non-structural protein , visible as minitubules after nucleation of the viroplasm .
We introduced Pns12 and Pns4-specific RNA interference ( RNAi ) constructs into rice plants .
The resultant transgenic plants accumulated short interfering RNAs specific to the constructs .
The progeny of rice plants with Pns12-specific RNAi constructs , after self-fertilization , were strongly resistant to viral infection .
By contrast , resistance was less apparent in the case of rice plants with Pns4-specific RNAi constructs , and delayed symptoms appeared in some plants of each line .
Our results suggest that interference with the expression of a protein that is critical for viral replication , such as the viroplasm matrix protein Pns12 , might be a practical and effective way to control viral infection in crop plants .
| Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: We introduced Pns12 and Pns4-specific RNA interference ( RNAi ) constructs into rice plants .
[ Sen. 1, subscore: 1.00 ]: The non-structural protein Pns12 of Rice dwarf virus is one of the early proteins expressed in cultured insect cells , and it is one of 12 proteins that initiate the formation of the viroplasm , the putative site of viral replication .
[ Sen. 2, subscore: 1.00 ]: Pns4 is also a non-structural protein , visible as minitubules after nucleation of the viroplasm .
[ Sen. 5, subscore: 1.00 ]: The progeny of rice plants with Pns12-specific RNAi constructs , after self-fertilization , were strongly resistant to viral infection .
[ Sen. 6, subscore: 1.00 ]: By contrast , resistance was less apparent in the case of rice plants with Pns4-specific RNAi constructs , and delayed symptoms appeared in some plants of each line .
[ Sen. 7, subscore: 1.00 ]: Our results suggest that interference with the expression of a protein that is critical for viral replication , such as the viroplasm matrix protein Pns12 , might be a practical and effective way to control viral infection in crop plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
