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Score: 14.00 |
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| Title: [ Analysis of the molecular motif for inducing response to jasmonic acid and ethylene in Pib promoter via rice transformation ] .
| | Author: Yu L Yang SH Jin YK Wan JM Zhao BQ | | Journal: Yi Chuan Citation: V : 32 P : 73-80 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20085889 Accession (PMID): 20085889 | | Abstract: The expression of Pib gene in rice was induced by hormone , such as jasmonic acid and ethylene .
In order to determine the necessary regions of sequence or motifs for response to jasmonic acid and ethylene in Pib promoter , the full length promoter of Pib ( -3 , 572 approximately 2 bp ) and three different 5 deletion fragments of Pib promoter ( -2 , 692 approximately 2 bp , -1 , 335 approximately 2 bp , -761 approximately 2 bp ) were synthesized by PCR and then were substituted for 35S upstream gus in a binary plasmid to construct re-combined plasmids of Pib promoter-gus fusions .
Transgenic rice plants of the four recombined plasmids were produced by Agrobacterium-mediated transformation .
Quality and quantum analysis of gus activities in transgenic plants at both protein and mRNA levels were conducted .
The promotion activity of the full length promoter of Pib ( -3 , 572 approximately 2 bp , pNAR901 ) was the highest in the four recombinants and the gus activities in its transgenic plant organs were enhanced obviously at 6 h after treatment with jasmonic acid or ethylene .
The promotion activity of the deleted Pib promoters was significantly decreased and the response to jasmonic acid or ethylene treatment was not present when the -3 , 572 approximately -2 , 692 bp sequence was knocked out from the Pib promoter .
Although the disparity in the lengths of the deleted Pib promoter of pNAR902 ( -2 , 692 approximately 2 bp ) , pNAR903 ( -1 , 335 approximately 2 bp ) , and pNAR904 ( -761 approximately 2 bp ) was more than 2 or 3 times , the response to jasmonic acid or ethylene treatment was not different among their transgenic plants .
All these results indicated that the common deleted sequences ( -3 , 572 approximately -2 , 692 bp ) in the three deleted Pib promoter constructs were the essential region to the response to jasmonic acid and ethylene treatment .
The result of pib promoter sequence searching indicated that there was only one GCCGCC motif at -2 , 722 bp of this common deleted segment in the Pib promoter sequence .
Our rice transgenic results showed that the GCCGCC may be a cis-motif for Pib gene conferring response to jasmonic acid and ethylene for Pib gene .
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[ Sen. 2, subscore: 4.00 ]: In order to determine the necessary regions of sequence or motifs for response to jasmonic acid and ethylene in Pib promoter , the full length promoter of Pib ( -3 , 572 approximately 2 bp ) and three different 5 deletion fragments of Pib promoter ( -2 , 692 approximately 2 bp , -1 , 335 approximately 2 bp , -761 approximately 2 bp ) were synthesized by PCR and then were substituted for 35S upstream gus in a binary plasmid to construct re-combined plasmids of Pib promoter-gus fusions .
[ Sen. 6, subscore: 2.00 ]: The promotion activity of the deleted Pib promoters was significantly decreased and the response to jasmonic acid or ethylene treatment was not present when the -3 , 572 approximately -2 , 692 bp sequence was knocked out from the Pib promoter .
[ Sen. 9, subscore: 2.00 ]: The result of pib promoter sequence searching indicated that there was only one GCCGCC motif at -2 , 722 bp of this common deleted segment in the Pib promoter sequence .
[ Sen. 10, subscore: 2.00 ]: Our rice transgenic results showed that the GCCGCC may be a cis-motif for Pib gene conferring response to jasmonic acid and ethylene for Pib gene .
[ Sen. 1, subscore: 1.00 ]: The expression of Pib gene in rice was induced by hormone , such as jasmonic acid and ethylene .
[ Sen. 5, subscore: 1.00 ]: The promotion activity of the full length promoter of Pib ( -3 , 572 approximately 2 bp , pNAR901 ) was the highest in the four recombinants and the gus activities in its transgenic plant organs were enhanced obviously at 6 h after treatment with jasmonic acid or ethylene .
[ Sen. 7, subscore: 1.00 ]: Although the disparity in the lengths of the deleted Pib promoter of pNAR902 ( -2 , 692 approximately 2 bp ) , pNAR903 ( -1 , 335 approximately 2 bp ) , and pNAR904 ( -761 approximately 2 bp ) was more than 2 or 3 times , the response to jasmonic acid or ethylene treatment was not different among their transgenic plants .
[ Sen. 8, subscore: 1.00 ]: All these results indicated that the common deleted sequences ( -3 , 572 approximately -2 , 692 bp ) in the three deleted Pib promoter constructs were the essential region to the response to jasmonic acid and ethylene treatment .
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Score: 11.00 |
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| Title: Expression of the Pib rice-blast-resistance gene family is up-regulated by environmental conditions favouring infection and by chemical signals that trigger secondary plant defences .
| | Author: Wang ZX Yamanouchi U Katayose Y Sasaki T Yano M | | Journal: Plant Mol . Biol . Citation: V : 47 ( 5 ) P : 653-61 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11725950 Accession (PMID): 11725950 | | Abstract: The rice blast resistance gene Pib is a member of the nucleotide binding site ( NBS ) and leucine-rich repeat ( LRR ) class of plant disease resistance ( R ) genes and belongs to a small gene family .
We describe here the isolation and characterization of a Pib homologue ( PibH8 ) , and extensive investigation of the expression of the Pib gene family ( Pib , PibH8 , HPibH8-1 , HPibH8-2 ) under various environmental and chemical treatments .
PibH8 shows 42% identity and 60% similarity to Pib and , like Pib , has a duplication of the kinase 1a , 2 , and 3a motifs of the NBS region in the N-terminal half of the protein .
Interestingly , genes of the Pib family exhibit a diurnal rhythm of expression .
RNA gel blot analysis revealed that their expression was regulated dramatically by environmental signals . such as temperature , light and water availability .
Their expression was also induced by chemical treatments , such as jasmonic acid , salicylic acid , ethylene and probenazole .
Our findings suggest that expression of the Pib gene family is up-regulated by environmental conditions that would favour pathogen infection .
This may reflect the evolution of anticipatory control of R gene expression . | Matching Sentences:
[ Sen. 2, subscore: 5.00 ]: We describe here the isolation and characterization of a Pib homologue ( PibH8 ) , and extensive investigation of the expression of the Pib gene family ( Pib , PibH8 , HPibH8-1 , HPibH8-2 ) under various environmental and chemical treatments .
[ Sen. 3, subscore: 3.00 ]: PibH8 shows 42% identity and 60% similarity to Pib and , like Pib , has a duplication of the kinase 1a , 2 , and 3a motifs of the NBS region in the N-terminal half of the protein .
[ Sen. 1, subscore: 1.00 ]: The rice blast resistance gene Pib is a member of the nucleotide binding site ( NBS ) and leucine-rich repeat ( LRR ) class of plant disease resistance ( R ) genes and belongs to a small gene family .
[ Sen. 4, subscore: 1.00 ]: Interestingly , genes of the Pib family exhibit a diurnal rhythm of expression .
[ Sen. 7, subscore: 1.00 ]: Our findings suggest that expression of the Pib gene family is up-regulated by environmental conditions that would favour pathogen infection .
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Score: 8.00 |
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| Title: Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice .
| | Author: Li Y Xia Q Kou H Wang D Lin X Wu Y Xu C Xing S Liu B | | Journal: J Integr Plant Biol Citation: V : P : Year: 2011 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub21781278 Accession (PMID): 21781278 | | Abstract: Pib is a well-characterized rice blast-resistance gene belonging to the nucleotide binding site ( NBS ) and leucine-rich repeat ( LRR ) superfamily .
Expression of Pib was low under non-challenged conditions , but strongly induced by the blast-causing fungal pathogen Magnaporthe grisea , thereby conferring resistance to the pathogen .
It is generally established that cytosine methylation of the promoter-region often plays a repressive role in modulating expression of the gene in question .
We report here that two critical regions of the Pib promoter were heavily CG cytosine-methylated in both cultivars studied .
Surprisingly , induced expression of Pib by M grisea infection did not entail its promoter demethylation , and partial demethylation by 5-azacytidine-treatment actually reduced Pib expression relative to wild-type plants .
Accordingly , the blast disease-resistance was compromised in the 5-azaC-treated plants relative to wild-type .
In contrast , the disease susceptibility was not affected by the 5-azaC treatment in another two rice cultivars that did not contain the Pib gene , ruling out effects of other R genes and non-specific genotoxic effects by the drug-treatment as a cause for the compromised Pib-conditioned blast-resistance .
Taken together , our results suggest that promoter DNA methylation plays a novel enhancing role in conditioning high-level of induced expression of the Pib gene in times of M grisea infection , and its conferred resistance to the pathogen .
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[ Sen. 5, subscore: 2.00 ]: Surprisingly , induced expression of Pib by M grisea infection did not entail its promoter demethylation , and partial demethylation by 5-azacytidine-treatment actually reduced Pib expression relative to wild-type plants .
[ Sen. 7, subscore: 2.00 ]: In contrast , the disease susceptibility was not affected by the 5-azaC treatment in another two rice cultivars that did not contain the Pib gene , ruling out effects of other R genes and non-specific genotoxic effects by the drug-treatment as a cause for the compromised Pib-conditioned blast-resistance .
[ Sen. 1, subscore: 1.00 ]: Pib is a well-characterized rice blast-resistance gene belonging to the nucleotide binding site ( NBS ) and leucine-rich repeat ( LRR ) superfamily .
[ Sen. 2, subscore: 1.00 ]: Expression of Pib was low under non-challenged conditions , but strongly induced by the blast-causing fungal pathogen Magnaporthe grisea , thereby conferring resistance to the pathogen .
[ Sen. 4, subscore: 1.00 ]: We report here that two critical regions of the Pib promoter were heavily CG cytosine-methylated in both cultivars studied .
[ Sen. 8, subscore: 1.00 ]: Taken together , our results suggest that promoter DNA methylation plays a novel enhancing role in conditioning high-level of induced expression of the Pib gene in times of M grisea infection , and its conferred resistance to the pathogen .
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Score: 7.00 |
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| Title: Identification of the rice blast resistance gene Pib in the National Small Grains Collection .
| | Author: Roychowdhury M Jia Y Jia MH Fjellstrom R Cartwright RD | | Journal: Phytopathology Citation: V : 102 P : 700-6 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22667447 Accession (PMID): 22667447 | | Abstract: The Pib gene in rice confers resistance to a wide range of races of the rice blast pathogen , Magnaporthe oryzae , including race IE1k that overcomes Pita , another broad-spectrum resistance gene .
In this study , the presence of Pib was determined in 164 rice germplasm accessions from a core subset of the National Small Grains Collection utilizing DNA markers and pathogenicity assays .
The presence of Pib was evaluated with two simple sequence repeat ( SSR ) markers and a dominant marker ( Pib-dom ) derived from the Pib gene sequence .
Pathogenicity assays using two avirulent races ( IE1k and IB1 ) and a virulent race ( IB54 ) were performed to verify the resistance responses of accessions .
Of the 164 accessions evaluated , 109 contained the Pib gene as determined using both SSR markers and pathogenicity assays , albeit different haplotypes were detected .
The remaining 52 germplasm accessions were different in their responses to the blast races IB54 , IE1k , and IB1 , thus indicating the presence of R gene ( s ) other than Pib .
The accessions characterized in this study could be used for marker-assisted breeding to improve blast resistance in indica and japonica cultivars worldwide .
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[ Sen. 3, subscore: 3.00 ]: The presence of Pib was evaluated with two simple sequence repeat ( SSR ) markers and a dominant marker ( Pib-dom ) derived from the Pib gene sequence .
[ Sen. 1, subscore: 1.00 ]: The Pib gene in rice confers resistance to a wide range of races of the rice blast pathogen , Magnaporthe oryzae , including race IE1k that overcomes Pita , another broad-spectrum resistance gene .
[ Sen. 2, subscore: 1.00 ]: In this study , the presence of Pib was determined in 164 rice germplasm accessions from a core subset of the National Small Grains Collection utilizing DNA markers and pathogenicity assays .
[ Sen. 5, subscore: 1.00 ]: Of the 164 accessions evaluated , 109 contained the Pib gene as determined using both SSR markers and pathogenicity assays , albeit different haplotypes were detected .
[ Sen. 6, subscore: 1.00 ]: The remaining 52 germplasm accessions were different in their responses to the blast races IB54 , IE1k , and IB1 , thus indicating the presence of R gene ( s ) other than Pib .
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Score: 6.00 |
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| Title: The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes .
| | Author: Wang ZX Yano M Yamanouchi U Iwamoto M Monna L Hayasaka H Katayose Y Sasaki T | | Journal: Plant J Citation: V : 19 ( 1 ) P : 55-64 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10417726 Accession (PMID): 10417726 | | Abstract: Rice blast , caused by the fungal pathogen Magnaporthe grisea , is one of the most serious diseases of rice .
Here we describe the isolation and characterization of Pib , one of the rice blast resistance genes .
The Pib gene was isolated by a map-based cloning strategy .
The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site ( NBS ) and leucine-rich repeats ( LRRs ) ; thus , Pib is a member of the NBS-LRR class of plant disease resistance genes .
Interestingly , a duplication of the kinase 1a , 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein .
In addition , eight cysteine residues are clustered in the middle of the LRRs , a feature which has not been reported for other R genes .
Pib gene expression was induced upon altered environmental conditions , such as altered temperatures and darkness . | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site ( NBS ) and leucine-rich repeats ( LRRs ) ; thus , Pib is a member of the NBS-LRR class of plant disease resistance genes .
[ Sen. 2, subscore: 1.00 ]: Here we describe the isolation and characterization of Pib , one of the rice blast resistance genes .
[ Sen. 3, subscore: 1.00 ]: The Pib gene was isolated by a map-based cloning strategy .
[ Sen. 5, subscore: 1.00 ]: Interestingly , a duplication of the kinase 1a , 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein .
[ Sen. 7, subscore: 1.00 ]: Pib gene expression was induced upon altered environmental conditions , such as altered temperatures and darkness .
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Score: 5.00 |
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| Title: [ The inductive activation of the promoter of pib gene ] | | Author: Li CJ Yang SH Wu L Wan JM .
| | Journal: Yi Chuan Citation: V : 28 ( 6 ) P : 689-94 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16818431 Accession (PMID): 16818431 | | Abstract: A 5 . 7 kb putative promoter region of pib gene was isolated from the pib genomic clone and substituted for the 35S promoter upstream of gus gene in plasmid pCAMBIA1301 to construct a new plant expression vector pNAR604 ( putative pib promoter-GUS + 35S-hpt ) .
From Agrobacterium-mediated transformation and hygromycin selective culture in vitro , hygromycin resistant calli and 36 transgenic rice ( Oryza sativa L ) plants were obtained .
Histochemical assays of GUS activity showed that no expression was observed in the resistant calli and roots from transgenic rice if cultured under light , but after 24 h dark treatment there was strong GUS staining .
Fluorimetric quantitative analysis indicated that GUS expression was organ-specific in transgenic rice .
Without the dark treatment , GUS activity in roots and stems were about 7 and 3 times higher than in leaves in which GUS activity was only trace detected .
After 24 h dark treatment , GUS activity in roots , stems and leaves of transgenic plants were all promoted and the largest increase was observed in leaves .
Twenty-four hours after spraying with 5 mmol/L SA ( Salicylic Acid ) or 0 . 3 mol/L NaCl , GUS activity in leaves of the transgenic plants was 2 . 7 or 3 . 6 times respectively higher than untreated control .
It was confirmed that an inductive promoter was involved in this 5 . 7 kb upstream region of pib gene , and dark , SA and NaCl treatments were inductive factors for pib promoter . | Matching Sentences:
[ Sen. 1, subscore: 3.00 ]: A 5 . 7 kb putative promoter region of pib gene was isolated from the pib genomic clone and substituted for the 35S promoter upstream of gus gene in plasmid pCAMBIA1301 to construct a new plant expression vector pNAR604 ( putative pib promoter-GUS + 35S-hpt ) .
[ Sen. 8, subscore: 2.00 ]: It was confirmed that an inductive promoter was involved in this 5 . 7 kb upstream region of pib gene , and dark , SA and NaCl treatments were inductive factors for pib promoter .
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Score: 4.00 |
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| Title: Characterization of rice mutants with enhanced susceptibility to rice blast | | Author: Kim HK Lee SK Cho JI Lee S An G Jwa NS Kim BR Cho YC Han SS Bhoo SH Lee YH Hong YK Yi G Park DS Hahn TR Jeon JS .
| | Journal: Mol .
Cells Citation: V : 20 ( 3 ) P : 385-91 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16404154 Accession (PMID): 16404154 | | Abstract: As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance , we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 ( 91-033 ) from a T-DNA insertion library of the japonica rice cultivar , Hwayeong .
Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning strategy to isolate the gene ( s ) responsible for the enhanced susceptibility of the Hwayeong mutants .
A genetic mapping population was produced by crossing the resistant wild type Hwayeong with the susceptible cultivar , Nagdong .
Chi-square analysis of the F2 segregating population indicated that resistance in Hwayeong was controlled by a single major gene that we tentatively named Pi-hy .
Randomly selected susceptible plants in the F2 population were used to build an initial map of Pi-hy .
The SSLP marker RM2265 on chromosome 2 was closely linked to resistance .
High resolution mapping using 105 F2 plants revealed that the resistance gene was tightly linked , or identical , to Pib , a resistance gene with a nucleotide binding sequence and leucine-rich repeats ( NB-LRR ) previously isolated .
Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene , demonstrating that the Pi-hy gene is Pib .
The Pib mutations in 1D-22-10-13 , 1D-54-16-8 , and 1C-143-16-1 were , respectively , a missense mutation in the conserved NB domain 3 , a nonsense mutation in the 5th LRR , and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus .
These findings provide evidence that NB domain 3 and the C terminus are required for full activity of the plant R gene .
They also suggest that alterations of the resistance gene can cause major differences in pathogen specificity by affecting interactions with an avirulence factor . | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene , demonstrating that the Pi-hy gene is Pib .
[ Sen. 7, subscore: 1.00 ]: High resolution mapping using 105 F2 plants revealed that the resistance gene was tightly linked , or identical , to Pib , a resistance gene with a nucleotide binding sequence and leucine-rich repeats ( NB-LRR ) previously isolated .
[ Sen. 9, subscore: 1.00 ]: The Pib mutations in 1D-22-10-13 , 1D-54-16-8 , and 1C-143-16-1 were , respectively , a missense mutation in the conserved NB domain 3 , a nonsense mutation in the 5th LRR , and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus .
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Score: 3.00 |
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| Title: [ Initial functional analysis of the promoter region and coding region of Pib gene in transgenic rice ] | | Author: Zhou M Yang SH Lan Y Jin YK Wan JM | | Journal: Yi Chuan Citation: V : 30 P : 367-72 Year: 2008 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18332008 Accession (PMID): 18332008 | | Abstract: The promoter region and intact coding region of Pib gene ( 9 . 9 kb ) was inserted into the downstream of CaMV 35S promoter in a binary vector pPZP2Ha3 ( + ) , resulting a plasmid pNAR701 .
And a fragment of Pib gene from 6 986 to 9 392 bp was placed into pPZP2Ha3 ( - ) under the control of CaMV 35S promoter , producing an antisense expression vector pNAR703 .
These two recombined vectors were transferred into a blast medium susceptible rice cultivar R109 by an Agrobecterium-mediated method .
Tests of PCR and Southern blotting for transgenic plants as well as the segregation of hygromycin resistance in T1 generation confirmed that the target DNA fragments were integrated into genome of R109 and hereditable .
Northern blotting analysis showed the coding region of Pib gene double driven by 35S and its native promoter was able to transcript in T1 transgenic plants .
Rice blast resistance test for T1 transgenic seedlings of 3-4 leaves stage and in vitro leaves in tillering stage showed that transgenic plants of pNAR701 were more resistant to blast race ZD1 and ZG1 than the wild type plants , but the resistance of antisense transgenic plants from pNAR703 was decreased compared to the controls .
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[ Sen. 1, subscore: 1.00 ]: The promoter region and intact coding region of Pib gene ( 9 . 9 kb ) was inserted into the downstream of CaMV 35S promoter in a binary vector pPZP2Ha3 ( + ) , resulting a plasmid pNAR701 .
[ Sen. 2, subscore: 1.00 ]: And a fragment of Pib gene from 6 986 to 9 392 bp was placed into pPZP2Ha3 ( - ) under the control of CaMV 35S promoter , producing an antisense expression vector pNAR703 .
[ Sen. 5, subscore: 1.00 ]: Northern blotting analysis showed the coding region of Pib gene double driven by 35S and its native promoter was able to transcript in T1 transgenic plants .
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Score: 3.00 |
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| Title: A rice blast-resistance genetic resource from wild rice in Yunnan , China .
| | Author: Yang MZ Cheng ZQ Chen SN Qian J Xu LL Huang XQ | | Journal: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao Citation: V : 33 P : 589-95 Year: 2007 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18349514 Accession (PMID): 18349514 | | Abstract: Compared to Pi-ta ( - ) alleles , Pi-ta ( + ) alleles can cause blast resistance response .
In this work , Pi-ta gene in multiple rice materials , including local rice cultivars , different types of O rufipogon and O longistaminata was detected by molecular cloning and sequence analysis .
Results indicated that Pi-ta ( + ) alleles were rare alleles , because in all the tested materials , only the Erect type of O rufipogon ( ETOR ) from Jinghong county in Yunnan province contains a Pi-ta ( + ) allele .
Another rice blast resistance gene , Pib , confers resistance to the Japanese strain of M grisea , was also confirmed to be functional in this type of O rufipogon .
The results of pathogen inoculation test show that ETOR is more strongly resistant to the tested blast pathogen races than other types of O rufipogon .
The resistance of ETOR may at least partially depend upon the functioning of Pi-ta and Pib gene .
As O rufipogon has the same type of genome with the cultivated rice ( O sativa ) , Pi-ta ( + ) and Pib gene in Erect type of O rufipogon can be used to improve the tolerance of cultivated rice to blast , either by traditional hybridization or by genetic engineering .
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[ Sen. 4, subscore: 1.00 ]: Another rice blast resistance gene , Pib , confers resistance to the Japanese strain of M grisea , was also confirmed to be functional in this type of O rufipogon .
[ Sen. 6, subscore: 1.00 ]: The resistance of ETOR may at least partially depend upon the functioning of Pi-ta and Pib gene .
[ Sen. 7, subscore: 1.00 ]: As O rufipogon has the same type of genome with the cultivated rice ( O sativa ) , Pi-ta ( + ) and Pib gene in Erect type of O rufipogon can be used to improve the tolerance of cultivated rice to blast , either by traditional hybridization or by genetic engineering .
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Score: 1.00 |
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| Title: Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates .
| | Author: Wu JL Fan YY Li DB Zheng KL Leung H Zhuang JY .
| | Journal: Theor . Appl . Genet . Citation: V : 111 ( 1 ) P : 50-6 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15856160 Accession (PMID): 15856160 | | Abstract: To further our understanding of the genetic control of blast resistance in rice cultivar Gumei 2 and , consequently , to facilitate the utilization of this durably blast-resistant cultivar , we studied 304 recombinant inbred lines of indica rice cross Zhong 156/Gumei 2 and a linkage map comprising 181 markers .
An analysis of segregation for resistance against five isolates of rice blast suggested that one gene cluster and three additional major genes that are independently inherited are responsible for the complete resistance of Gumei 2 .
The gene cluster was located to chromosome 6 and includes two genes mapped previously , Pi25 ( t ) , against Chinese rice blast isolate 92-183 ( race ZC15 ) and Pi26 ( t ) against Philippine rice blast isolate Ca89 ( lineage 4 ) , and a gene for resistance against Philippine rice blast isolate 92330-5 ( lineage 17 ) .
Of the two genes conferring resistance against the Philippine isolates V86013 ( lineage 15 ) and C923-39 ( lineage 46 ) , we identified one as Pi26 ( t ) and mapped the other onto the distal end of chromosome 2 where Pib is located .
We used three components of partial blast resistance , percentage diseased leaf area ( DLA ) , lesion number and lesion size , all measured in the greenhouse , to measure the degree of susceptibility to isolates Ca89 and C923-39 and subsequently identified nine and eight quantitative trait loci ( QTLs ) , respectively .
Epistasis was determined to play an important role in partial resistance against Ca89 .
Using DLA measured on lines susceptible in a blast nursery , we detected six QTLs .
While different QTLs were detected for partial resistance to Ca89 and C923-39 , respectively , most were involved in the partial resistance in the field .
Our results suggest that the blast resistance in Gumei 2 is controlled by multiple major genes and minor genes with epistatic effects . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Of the two genes conferring resistance against the Philippine isolates V86013 ( lineage 15 ) and C923-39 ( lineage 46 ) , we identified one as Pi26 ( t ) and mapped the other onto the distal end of chromosome 2 where Pib is located .
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