45 matches found in 19 documents. Search time: 0.01 seconds. |
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Score: 11.00 | Title: Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes .
| Author: Lee SK Song MY Seo YS Kim HK Ko S Cao PJ Suh JP Yi G Roh JH Lee S An G Hahn TR Wang GL Ronald P Jeon JS | Journal: Genetics Citation: V : 181 P : 1627-38 Year: 2009 Type: In-Process | Literature: oryza Field: abstract Doc ID: pub19153255 Accession (PMID): 19153255 | Matching Sentences: [ Sen. 3, subscore: 4.00 ]: Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50 , a susceptible rice cultivar , and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval Sequence analysis of this genomic region identified two candidate genes , Pi5-1 and Pi5-2 , which encode proteins carrying three motifs characteristic of R genes : an N-terminal coiled-coil ( CC ) motif , a nucleotide-binding ( NB ) domain , and a leucine-rich repeat ( LRR ) motif . [ Sen. 4, subscore: 2.00 ]: In genetic transformation experiments of a susceptible rice cultivar , neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M oryzae . [ Sen. 6, subscore: 2.00 ]: Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge , whereas the Pi5-2 gene is constitutively expressed . [ Sen. 2, subscore: 1.00 ]: To understand the molecular basis of Pi5-mediated resistance to M oryzae , we cloned the resistance ( R ) gene at this locus using a map-based cloning strategy . [ Sen. 5, subscore: 1.00 ]: In contrast , transgenic rice plants expressing both of these genes , generated by crossing transgenic lines carrying each gene individually , conferred Pi5-mediated resistance to M oryzae . [ Sen. 7, subscore: 1.00 ]: These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M oryzae .
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Score: 9.00 | Title: A novel blast resistance gene , Pi54rh cloned from wild species of rice , Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae .
| Author: Das A Soubam D Singh PK Thakur S Singh NK Sharma TR | Journal: Funct Integr Genomics Citation: V : 12 P : 215-28 Year: 2012 Type: In-Process | Literature: oryza Field: abstract Doc ID: pub22592658 Accession (PMID): 22592658 | Matching Sentences: [ Sen. 2, subscore: 2.00 ]: In our effort to identify more effective forms of this gene , we isolated an orthologue of Pi54 named as Pi54rh from the blast-resistant wild species of rice , Oryza rhizomatis , using allele mining approach and validated by complementation . [ Sen. 1, subscore: 1.00 ]: The dominant rice blast resistance gene , Pi54 confers resistance to Magnaporthe oryzae in different parts of India . [ Sen. 3, subscore: 1.00 ]: The Pi54rh belongs to CC-NBS-LRR family of disease resistance genes with a unique Zinc finger ( C ( 3 ) H type ) domain . [ Sen. 4, subscore: 1.00 ]: The 1 , 447 bp Pi54rh transcript comprises of 101 bp 5-UTR , 1 , 083 bp coding region and 263 bp 3-UTR , driven by pathogen inducible promoter . [ Sen. 5, subscore: 1.00 ]: We showed the extracellular localization of Pi54rh protein and the presence of glycosylation , myristoylation and phosphorylation sites which implicates its role in signal transduction process . [ Sen. 7, subscore: 1.00 ]: The Pi54rh was found to express constitutively at basal level in the leaves , but upregulates 3 . 8-fold at 96 h post-inoculation with the pathogen . [ Sen. 8, subscore: 1.00 ]: Functional validation of cloned Pi54rh gene using complementation test showed high degree of resistance to seven isolates of M oryzae collected from different geographical locations of India . [ Sen. 9, subscore: 1.00 ]: In this study , for the first time , we demonstrated that a rice blast resistance gene Pi54rh cloned from wild species of rice provides broad spectrum resistance to M oryzae hence can be used in rice improvement breeding programme .
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Score: 7.00 | Title: Genetic and physical mapping of Pi5 ( t ) , a locus associated with broad-spectrum resistance to rice blast | Author: Jeon JS Chen D Yi GH Wang GL Ronald PC .
| Journal: Mol . Genet . Genomics Citation: V : 269 ( 2 ) P : 280-9 Year: 2003 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12756540 Accession (PMID): 12756540 | Matching Sentences: [ Sen. 9, subscore: 2.00 ]: DNA gel-blot analyses indicated that the region around Pi3 ( t ) is identical to that of Pi5 ( t ) , suggesting that Pi3 ( t ) and Pi5 ( t ) are the same resistance gene . [ Sen. 1, subscore: 1.00 ]: To gain an understanding of the molecular basis for resistance to rice blast ( Magnaporthe grisea ) , we have initiated a project to clone Pi5 ( t ) , a locus associated with broad-spectrum resistance to diverse blast isolates . [ Sen. 2, subscore: 1.00 ]: AFLP-derived markers linked to Pi5 ( t ) -mediated resistance were isolated using bulked segregant analysis of F ( 2 ) populations generated by crossing three recombinant inbred lines ( RILs ) , RIL125 , RIL249 , and RIL260 with the susceptible line CO39 . [ Sen. 6, subscore: 1.00 ]: Using these resources Pi5 ( t ) was mapped to a 170-kb interval , and a contiguous set of BIBAC clones spanning this region was constructed . [ Sen. 7, subscore: 1.00 ]: It had previously been suggested that Pi3 ( t ) and Pi5 ( t ) might be allelic , due to their identical resistance spectrum and tight linkage . [ Sen. 8, subscore: 1.00 ]: We therefore compared genomic regions for lines containing Pi3 ( t ) using the Pi5 ( t ) -linked markers .
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Score: 6.00 | Title: Use of Pi5 ( t ) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea .
| Author: Yi G Lee SK Hong YK Cho YC Nam MH Kim SC Han SS Wang GL Hahn TR Ronald PC Jeon JS .
| Journal: Theor . Appl . Genet . Citation: V : 109 ( 5 ) P : 978-85 Year: 2004 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub15141293 Accession (PMID): 15141293 | Matching Sentences: [ Sen. 2, subscore: 1.00 ]: The rice Pi5 ( t ) locus confers broad-spectrum resistance to Magnaporthe grisea , the causal agent of rice blast disease . [ Sen. 3, subscore: 1.00 ]: It has been hypothesized that the Pi5 ( t ) locus carries the same gene as that encoded by the Pi3 ( t ) and Pii ( t ) loci . [ Sen. 4, subscore: 1.00 ]: We developed three PCR-based dominant markers ( JJ80-T3 , JJ81-T3 , and JJ113-T3 ) from three previously identified BIBAC clones-JJ80 , JJ81 , and JJ113-that are linked to the Pi5 ( t ) locus . [ Sen. 5, subscore: 1.00 ]: PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5 ( t ) , Pi3 ( t ) , and Pii ( t ) . [ Sen. 6, subscore: 1.00 ]: PCR and DNA gel-blot analysis of candidate resistance lines using JJ80-T3 , JJ81-T3 , and JJ113-T3 indicated that Tetep is the likely donor of Pi5 ( t ) . [ Sen. 9, subscore: 1.00 ]: The genomic structure of three of these resistant varieties ( ie , IR72 , Taebaeg , Jahyangdo ) is most similar to that of Pi5 ( t ) .
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Score: 3.00 | Title: Molecular mapping of the blast resistance genes Pi2-1 and Pi51 ( t ) in the durably resistant rice Tianjingyeshengdao .
| Author: Wang Y Wang D Deng X Liu J Sun P Liu Y Huang H Jiang N Kang H Ning Y Wang Z Xiao Y Liu X Liu E Dai L Wang GL | Journal: Phytopathology Citation: V : 102 P : 779-86 Year: 2012 Type: In-Process | Literature: oryza Field: abstract Doc ID: pub22779744 Accession (PMID): 22779744 | Matching Sentences: [ Sen. 5, subscore: 1.00 ]: Two QTL clusters on chromosome 6 and 12 were named Pi2-1 and Pi51 ( t ) , respectively . [ Sen. 7, subscore: 1.00 ]: Pi51 ( t ) was only detected in the natural blast nursery and explained 3 . 67 to 10 . 37% of the phenotypic variation . [ Sen. 9, subscore: 1.00 ]: Identification of the markers linked to both Pi2-1 and Pi51 ( t ) in this study should be useful for marker-aided selection in rice breeding programs as well as for molecular cloning of the identified resistance genes .
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