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45 matches found in 19 documents. Search time: 0.011 seconds.
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Score: 11.00
Title: Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes .
Author: Lee SK Song MY Seo YS Kim HK Ko S Cao PJ Suh JP Yi G Roh JH Lee S An G Hahn TR Wang GL Ronald P Jeon JS
Journal: Genetics Citation: V : 181 P : 1627-38 Year: 2009 Type: In-Process
Literature: oryza Field: abstract Doc ID: pub19153255 Accession (PMID): 19153255
Abstract: Rice blast , caused by the fungus Magnaporthe oryzae , is one of the most devastating diseases of rice . To understand the molecular basis of Pi5-mediated resistance to M oryzae , we cloned the resistance ( R ) gene at this locus using a map-based cloning strategy . Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50 , a susceptible rice cultivar , and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval Sequence analysis of this genomic region identified two candidate genes , Pi5-1 and Pi5-2 , which encode proteins carrying three motifs characteristic of R genes : an N-terminal coiled-coil ( CC ) motif , a nucleotide-binding ( NB ) domain , and a leucine-rich repeat ( LRR ) motif . In genetic transformation experiments of a susceptible rice cultivar , neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M oryzae . In contrast , transgenic rice plants expressing both of these genes , generated by crossing transgenic lines carrying each gene individually , conferred Pi5-mediated resistance to M oryzae . Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge , whereas the Pi5-2 gene is constitutively expressed . These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M oryzae .
Matching Sentences:
[ Sen. 3, subscore: 4.00 ]: Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50 , a susceptible rice cultivar , and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval Sequence analysis of this genomic region identified two candidate genes , Pi5-1 and Pi5-2 , which encode proteins carrying three motifs characteristic of R genes : an N-terminal coiled-coil ( CC ) motif , a nucleotide-binding ( NB ) domain , and a leucine-rich repeat ( LRR ) motif .
[ Sen. 4, subscore: 2.00 ]: In genetic transformation experiments of a susceptible rice cultivar , neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M oryzae .
[ Sen. 6, subscore: 2.00 ]: Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge , whereas the Pi5-2 gene is constitutively expressed .
[ Sen. 2, subscore: 1.00 ]: To understand the molecular basis of Pi5-mediated resistance to M oryzae , we cloned the resistance ( R ) gene at this locus using a map-based cloning strategy .
[ Sen. 5, subscore: 1.00 ]: In contrast , transgenic rice plants expressing both of these genes , generated by crossing transgenic lines carrying each gene individually , conferred Pi5-mediated resistance to M oryzae .
[ Sen. 7, subscore: 1.00 ]: These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M oryzae .
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Score: 9.00
Title: A novel blast resistance gene , Pi54rh cloned from wild species of rice , Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae .
Author: Das A Soubam D Singh PK Thakur S Singh NK Sharma TR
Journal: Funct Integr Genomics Citation: V : 12 P : 215-28 Year: 2012 Type: In-Process
Literature: oryza Field: abstract Doc ID: pub22592658 Accession (PMID): 22592658
Abstract: The dominant rice blast resistance gene , Pi54 confers resistance to Magnaporthe oryzae in different parts of India . In our effort to identify more effective forms of this gene , we isolated an orthologue of Pi54 named as Pi54rh from the blast-resistant wild species of rice , Oryza rhizomatis , using allele mining approach and validated by complementation . The Pi54rh belongs to CC-NBS-LRR family of disease resistance genes with a unique Zinc finger ( C ( 3 ) H type ) domain . The 1 , 447 bp Pi54rh transcript comprises of 101 bp 5-UTR , 1 , 083 bp coding region and 263 bp 3-UTR , driven by pathogen inducible promoter . We showed the extracellular localization of Pi54rh protein and the presence of glycosylation , myristoylation and phosphorylation sites which implicates its role in signal transduction process . This is in contrast to other blast resistance genes that are predicted to be intracellular NBS-LRR-type resistance proteins . The Pi54rh was found to express constitutively at basal level in the leaves , but upregulates 3 . 8-fold at 96 h post-inoculation with the pathogen . Functional validation of cloned Pi54rh gene using complementation test showed high degree of resistance to seven isolates of M oryzae collected from different geographical locations of India . In this study , for the first time , we demonstrated that a rice blast resistance gene Pi54rh cloned from wild species of rice provides broad spectrum resistance to M oryzae hence can be used in rice improvement breeding programme .
Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: In our effort to identify more effective forms of this gene , we isolated an orthologue of Pi54 named as Pi54rh from the blast-resistant wild species of rice , Oryza rhizomatis , using allele mining approach and validated by complementation .
[ Sen. 1, subscore: 1.00 ]: The dominant rice blast resistance gene , Pi54 confers resistance to Magnaporthe oryzae in different parts of India .
[ Sen. 3, subscore: 1.00 ]: The Pi54rh belongs to CC-NBS-LRR family of disease resistance genes with a unique Zinc finger ( C ( 3 ) H type ) domain .
[ Sen. 4, subscore: 1.00 ]: The 1 , 447 bp Pi54rh transcript comprises of 101 bp 5-UTR , 1 , 083 bp coding region and 263 bp 3-UTR , driven by pathogen inducible promoter .
[ Sen. 5, subscore: 1.00 ]: We showed the extracellular localization of Pi54rh protein and the presence of glycosylation , myristoylation and phosphorylation sites which implicates its role in signal transduction process .
[ Sen. 7, subscore: 1.00 ]: The Pi54rh was found to express constitutively at basal level in the leaves , but upregulates 3 . 8-fold at 96 h post-inoculation with the pathogen .
[ Sen. 8, subscore: 1.00 ]: Functional validation of cloned Pi54rh gene using complementation test showed high degree of resistance to seven isolates of M oryzae collected from different geographical locations of India .
[ Sen. 9, subscore: 1.00 ]: In this study , for the first time , we demonstrated that a rice blast resistance gene Pi54rh cloned from wild species of rice provides broad spectrum resistance to M oryzae hence can be used in rice improvement breeding programme .
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Score: 7.00
Title: Genetic and physical mapping of Pi5 ( t ) , a locus associated with broad-spectrum resistance to rice blast
Author: Jeon JS Chen D Yi GH Wang GL Ronald PC .
Journal: Mol . Genet . Genomics Citation: V : 269 ( 2 ) P : 280-9 Year: 2003 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub12756540 Accession (PMID): 12756540
Abstract: To gain an understanding of the molecular basis for resistance to rice blast ( Magnaporthe grisea ) , we have initiated a project to clone Pi5 ( t ) , a locus associated with broad-spectrum resistance to diverse blast isolates . AFLP-derived markers linked to Pi5 ( t ) -mediated resistance were isolated using bulked segregant analysis of F ( 2 ) populations generated by crossing three recombinant inbred lines ( RILs ) , RIL125 , RIL249 , and RIL260 with the susceptible line CO39 . The most tightly linked AFLP marker , S04G03 , was positioned on chromosome 9 of the fingerprint-based physical map of Nipponbare , a well-characterized rice genotype . Flanking BAC-based Nipponbare markers were generated for saturation mapping using four populations , the three initial RILs and an additional one derived from a cross between M202 and RIL260 . A BIBAC ( binary BAC ) library was constructed from RIL260 . Using these resources Pi5 ( t ) was mapped to a 170-kb interval , and a contiguous set of BIBAC clones spanning this region was constructed . It had previously been suggested that Pi3 ( t ) and Pi5 ( t ) might be allelic , due to their identical resistance spectrum and tight linkage . We therefore compared genomic regions for lines containing Pi3 ( t ) using the Pi5 ( t ) -linked markers . DNA gel-blot analyses indicated that the region around Pi3 ( t ) is identical to that of Pi5 ( t ) , suggesting that Pi3 ( t ) and Pi5 ( t ) are the same resistance gene .
Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: DNA gel-blot analyses indicated that the region around Pi3 ( t ) is identical to that of Pi5 ( t ) , suggesting that Pi3 ( t ) and Pi5 ( t ) are the same resistance gene .
[ Sen. 1, subscore: 1.00 ]: To gain an understanding of the molecular basis for resistance to rice blast ( Magnaporthe grisea ) , we have initiated a project to clone Pi5 ( t ) , a locus associated with broad-spectrum resistance to diverse blast isolates .
[ Sen. 2, subscore: 1.00 ]: AFLP-derived markers linked to Pi5 ( t ) -mediated resistance were isolated using bulked segregant analysis of F ( 2 ) populations generated by crossing three recombinant inbred lines ( RILs ) , RIL125 , RIL249 , and RIL260 with the susceptible line CO39 .
[ Sen. 6, subscore: 1.00 ]: Using these resources Pi5 ( t ) was mapped to a 170-kb interval , and a contiguous set of BIBAC clones spanning this region was constructed .
[ Sen. 7, subscore: 1.00 ]: It had previously been suggested that Pi3 ( t ) and Pi5 ( t ) might be allelic , due to their identical resistance spectrum and tight linkage .
[ Sen. 8, subscore: 1.00 ]: We therefore compared genomic regions for lines containing Pi3 ( t ) using the Pi5 ( t ) -linked markers .
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Score: 6.00
Title: Use of Pi5 ( t ) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea .
Author: Yi G Lee SK Hong YK Cho YC Nam MH Kim SC Han SS Wang GL Hahn TR Ronald PC Jeon JS .
Journal: Theor . Appl . Genet . Citation: V : 109 ( 5 ) P : 978-85 Year: 2004 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub15141293 Accession (PMID): 15141293
Abstract: Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for marker-assisted selection ( MAS ) . The rice Pi5 ( t ) locus confers broad-spectrum resistance to Magnaporthe grisea , the causal agent of rice blast disease . It has been hypothesized that the Pi5 ( t ) locus carries the same gene as that encoded by the Pi3 ( t ) and Pii ( t ) loci . We developed three PCR-based dominant markers ( JJ80-T3 , JJ81-T3 , and JJ113-T3 ) from three previously identified BIBAC clones-JJ80 , JJ81 , and JJ113-that are linked to the Pi5 ( t ) locus . PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5 ( t ) , Pi3 ( t ) , and Pii ( t ) . PCR and DNA gel-blot analysis of candidate resistance lines using JJ80-T3 , JJ81-T3 , and JJ113-T3 indicated that Tetep is the likely donor of Pi5 ( t ) . Of the 184 rice varieties tested , 34 carried the JJ80-T3- , JJ81-T3- , and JJ113-T3-specific bands . Disease evaluation of those 34 varieties revealed that all conferred resistance to PO6-6 . The genomic structure of three of these resistant varieties ( ie , IR72 , Taebaeg , Jahyangdo ) is most similar to that of Pi5 ( t ) . Our results demonstrate the usefulness of the JJ80-T3 , JJ81-T3 , and JJ113-T3 markers for MAS for M grisea resistance .
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The rice Pi5 ( t ) locus confers broad-spectrum resistance to Magnaporthe grisea , the causal agent of rice blast disease .
[ Sen. 3, subscore: 1.00 ]: It has been hypothesized that the Pi5 ( t ) locus carries the same gene as that encoded by the Pi3 ( t ) and Pii ( t ) loci .
[ Sen. 4, subscore: 1.00 ]: We developed three PCR-based dominant markers ( JJ80-T3 , JJ81-T3 , and JJ113-T3 ) from three previously identified BIBAC clones-JJ80 , JJ81 , and JJ113-that are linked to the Pi5 ( t ) locus .
[ Sen. 5, subscore: 1.00 ]: PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5 ( t ) , Pi3 ( t ) , and Pii ( t ) .
[ Sen. 6, subscore: 1.00 ]: PCR and DNA gel-blot analysis of candidate resistance lines using JJ80-T3 , JJ81-T3 , and JJ113-T3 indicated that Tetep is the likely donor of Pi5 ( t ) .
[ Sen. 9, subscore: 1.00 ]: The genomic structure of three of these resistant varieties ( ie , IR72 , Taebaeg , Jahyangdo ) is most similar to that of Pi5 ( t ) .
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Score: 3.00
Title: Molecular mapping of the blast resistance genes Pi2-1 and Pi51 ( t ) in the durably resistant rice Tianjingyeshengdao .
Author: Wang Y Wang D Deng X Liu J Sun P Liu Y Huang H Jiang N Kang H Ning Y Wang Z Xiao Y Liu X Liu E Dai L Wang GL
Journal: Phytopathology Citation: V : 102 P : 779-86 Year: 2012 Type: In-Process
Literature: oryza Field: abstract Doc ID: pub22779744 Accession (PMID): 22779744
Abstract: Tianjingyeshengdao ( TY ) is a rice cultivar with durable resistance to populations of Magnaporthe oryzae ( the causal agent of blast ) in China . To understand the genetic basis of its resistance to blast , we developed a population of recombinant inbred lines from a cross between TY and the highly susceptible CO39 for gene mapping analysis . In total , 22 quantitative trait loci ( QTLs ) controlling rice blast resistance were identified on chromosomes 1 , 3 , 4 , 5 , 6 , 9 , 11 , and 12 from the evaluation of four disease parameters in both greenhouse and blast nursery conditions . Among these QTLs , 19 were contributed by TY and three by CO39 . Two QTL clusters on chromosome 6 and 12 were named Pi2-1 and Pi51 ( t ) , respectively . Pi2-1 was detected under both growth chamber and natural blast nursery conditions , and explained 31 . 24 to 59 . 73% of the phenotypic variation . Pi51 ( t ) was only detected in the natural blast nursery and explained 3 . 67 to 10 . 37% of the phenotypic variation . Our results demonstrate that the durable resistance in TY is controlled by two major and seven minor genes . Identification of the markers linked to both Pi2-1 and Pi51 ( t ) in this study should be useful for marker-aided selection in rice breeding programs as well as for molecular cloning of the identified resistance genes .
Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Two QTL clusters on chromosome 6 and 12 were named Pi2-1 and Pi51 ( t ) , respectively .
[ Sen. 7, subscore: 1.00 ]: Pi51 ( t ) was only detected in the natural blast nursery and explained 3 . 67 to 10 . 37% of the phenotypic variation .
[ Sen. 9, subscore: 1.00 ]: Identification of the markers linked to both Pi2-1 and Pi51 ( t ) in this study should be useful for marker-aided selection in rice breeding programs as well as for molecular cloning of the identified resistance genes .
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Score: 2.00
Title: The single functional blast resistance gene Pi54 activates a complex defence mechanism in rice .
Author: Gupta SK Rai AK Kanwar SS Chand D Singh NK Sharma TR
Journal: J Exp Bot Citation: V : 63 P : 757-72 Year: 2012 Type: In-Data-Review
Literature: oryza Field: abstract Doc ID: pub22058403 Accession (PMID): 22058403
Abstract: The Pi54 gene ( Pi-k ( h ) ) confers a high degree of resistance to diverse strains of the fungus Magnaporthe oryzae . In order to understand the genome-wide co-expression of genes in the transgenic rice plant Taipei 309 ( TP ) containing the Pi54 gene , microarray analysis was performed at 72 h post-inoculation of the M oryzae strain PLP-1 . A total of 1154 differentially expressing genes were identified in TP-Pi54 plants . Of these , 587 were up-regulated , whereas 567 genes were found to be down-regulated . 107 genes were found that were exclusively up-regulated and 58 genes that were down regulated in the case of TP-Pi54 . Various defence response genes , such as callose , laccase , PAL , and peroxidase , and genes related to transcription factors like NAC6 , Dof zinc finger , MAD box , bZIP , and WRKY were found to be up-regulated in the transgenic line . The enzymatic activities of six plant defence response enzymes , such as peroxidase , polyphenol oxidase , phenylalanine ammonia lyase , beta-glucosidase , beta-1 , 3-glucanase , and chitinase , were found to be significantly high in TP-Pi54 at different stages of inoculation by M oryzae . The total phenol content also increased significantly in resistant transgenic plants after pathogen inoculation . This study suggests the activation of defence response and transcription factor-related genes and a higher expression of key enzymes involved in the defence response pathway in the rice line TP-Pi54 , thus leading to incompatible host-pathogen interaction .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The Pi54 gene ( Pi-k ( h ) ) confers a high degree of resistance to diverse strains of the fungus Magnaporthe oryzae .
[ Sen. 2, subscore: 1.00 ]: In order to understand the genome-wide co-expression of genes in the transgenic rice plant Taipei 309 ( TP ) containing the Pi54 gene , microarray analysis was performed at 72 h post-inoculation of the M oryzae strain PLP-1 .
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Score: 2.00
Title: Identification of the novel recessive gene pi55 ( t ) conferring resistance to Magnaporthe oryzae .
Author: He X Liu X Wang L Wang L Lin F Cheng Y Chen Z Liao Y Pan Q
Journal: Sci China Life Sci Citation: V : 55 P : 141-9 Year: 2012 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub22415685 Accession (PMID): 22415685
Abstract: The elite rice cultivar Yuejingsimiao 2 ( YJ2 ) is characterized by a high level of grain quality and yield , and resistance against Magnaporthe oryzae . YJ2 showed 100% resistance to four fungal populations collected from Guangdong , Sichuan , Liaoning , and Heilongjiang Provinces , which is a higher frequency than that shown by the well-known resistance ( R ) gene donor cultivars such as Sanhuangzhan 2 and 28zhan . Segregation analysis for resistance with F ( 2 ) and F ( 4 ) populations indicated the resistance of YJ2 was controlled by multiple genes that are dominant or recessive . The putative R genes of YJ2 were roughly tagged by SSR markers , located on chromosomes 2 , 6 , 8 , and 12 , in a bulked-segregant analysis using genome-wide selected SSR markers with F ( 4 ) lines that segregated into 3 resistant ( R ) : 1 susceptible ( S ) or 1R : 3S . The recessive R gene on chromosome 8 was further mapped to an interval approximately 1 . 9 cM/152 kb in length by linkage analysis with genomic position-ready markers in the mapping population derived from an F ( 4 ) line that segregated into 1R : 3S . Given that no major R gene was mapped to this interval , the novel R gene was designated as pi55 ( t ) . Out of 26 candidate genes predicted in the region based on the reference genomic sequence of the cultivar Nipponbare , two genes that encode a leucine-rich repeat-containing protein and heavy-metal-associated domain-containing protein , respectively , were suggested as the most likely candidates for pi55 ( t ) .
Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Given that no major R gene was mapped to this interval , the novel R gene was designated as pi55 ( t ) .
[ Sen. 7, subscore: 1.00 ]: Out of 26 candidate genes predicted in the region based on the reference genomic sequence of the cultivar Nipponbare , two genes that encode a leucine-rich repeat-containing protein and heavy-metal-associated domain-containing protein , respectively , were suggested as the most likely candidates for pi55 ( t ) .
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Score: 1.00
Title: Quantitative trait loci associated with leaf and neck blast resistance in recombinant inbred line population of rice ( Oryza sativa ) .
Author: Sirithunya P Tragoonrung S Vanavichit A Pa-In N Vongsaprom C Toojinda T
Journal: DNA Res . Citation: V : 9 ( 3 ) P : 79-88 Year: 2002 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub12168952 Accession (PMID): 12168952
Abstract: Blast is an economically important disease of rice . To map genes controlling blast resistance , recombinant inbred lines ( RIL ) were developed from Khao Dawk Mali 105 , an aromatic , blast-susceptible cultivar and the blast resistance donor , CT 9993-5-10-M ( CT ) . A linkage map encompassing 2112 cM was constructed from 141 RILs using 90 restriction fragment length polymorphisms ( RFLPs ) and 31 simple sequence repeats ( SSR ) . Virulent isolates of blast fungus were identified by screening differential host sets against 87 single-spore isolates collected from the north and northeast of Thailand . Fifteen virulent blast isolates were selected for leaf blast screening . Neck blast was evaluated both under natural conditions and controlled inoculations . Quantitative trait loci ( QTLs ) for broad resistance spectrum ( BRS ) to leaf blast were located on chromosomes 7 and 9 . In particular , the QTL ( ch9 ) was mapped near the Pi5 ( t ) locus . The QTL ( ch7 ) was located close to a previously mapped partial resistance QTL . Both loci showed significant allelic interaction . Genotypes having CT alleles at both QTL ( ch7 ) and QTL ( ch9 ) were the most resistant . Two neck-blast QTLs were mapped on chromosomes 5 and 6 . The inconsistent map locations between the leaf and neck blast QTLs indicate the complexity of fixing both leaf and neck blast resistance . The coincidence of BRS and field resistance QTLs on chromosome 7 supports the idea that BRS may reflect the broad resistance spectrum to leaf blast in rice . These findings laid the foundation for the development of a marker-assisted scheme for improving Khoa Dawk Mali 105 and the majority of aromatic Thai rice varieties that are susceptible to blast
Matching Sentences:
[ Sen. 8, subscore: 1.00 ]: In particular , the QTL ( ch9 ) was mapped near the Pi5 ( t ) locus .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: The highly charged region of plant beta-type phosphatidylinositol 4-kinase is involved in membrane targeting and phospholipid binding .
Author: Lou Y Ma H Lin WH Chu ZQ Mueller-Roeber B Xu ZH Xue HW .
Journal: Plant Mol . Biol . Citation: V : 60 ( 5 ) P : 729-46 Year: 2006 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub16649109 Accession (PMID): 16649109
Abstract: In Arabidopsis thaliana and Oryza sativa , two types of PI 4-kinase ( PI4Ks ) have been isolated and functionally characterized . The alpha-type PI4Ks ( approximately 220 kDa ) contain a PH domain , which is lacking in beta-type PI4Ks ( approximately 120 kDa ) . Beta-type PI4Ks , exemplified by Arabidopsis AtPI4Kbeta and rice OsPI4K2 , contain a highly charged repetitive segment designated PPC ( Plant PI4K Charged ) region , which is an unique domain only found in plant beta-type PI4Ks at present . The PPC region has a length of approximately 300 amino acids and harboring 11 ( AtPI4Kbeta ) and 14 ( OsPI4K2 ) repeats , respectively , of a 20-aa motif . Studies employing a modified yeast-based "Sequence of Membrane-Targeting Detection" system demonstrate that the PPC ( OsPI4K2 ) region , as well as the former 8 and latter 6 repetitive motifs within the PPC region , are able to target fusion proteins to the plasma membrane . Further detection on the transiently expressed GFP fusion proteins in onion epidermal cells showed that the PPC ( OsPI4K2 ) region alone , as well as the region containing repetitive motifs 1-8 , was able to direct GFP to the plasma membrane , while the regions containing less repetitive motifs , ie 6 , 4 , 2 or single motif ( s ) led to predominantly intracellular localization . Agrobacterium-mediated transient expression of PPC-GFP fusion protein further confirms the membrane-targeting capacities of PPC region . In addition , the predominant plasma membrane localization of AtPI4Kbeta was mediated by the PPC region . Recombinant PPC peptide , expressed in E coli , strongly binds phosphatidic acid , PI and PI4P , but not phosphatidylcholine , PI5P , or PI ( 4 , 5 ) P2 in vitro , providing insights into potential mechanisms for regulating sub-cellular localization and lipid binding for the plant beta-type PI4Ks .
Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: Recombinant PPC peptide , expressed in E coli , strongly binds phosphatidic acid , PI and PI4P , but not phosphatidylcholine , PI5P , or PI ( 4 , 5 ) P2 in vitro , providing insights into potential mechanisms for regulating sub-cellular localization and lipid binding for the plant beta-type PI4Ks .
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Score: 1.00
Title: The identification of Pi50 ( t ) , a new member of the rice blast resistance Pi2/Pi9 multigene family .
Author: Zhu X Chen S Yang J Zhou S Zeng L Han J Su J Wang L Pan Q
Journal: Theor Appl Genet Citation: V : 124 P : 1295-304 Year: 2012 Type: In-Process
Literature: oryza Field: abstract Doc ID: pub22270148 Accession (PMID): 22270148
Abstract: The deployment of broad-spectrum resistance genes is the most effective and economic means of controlling blast in rice . The cultivar Er-Ba-Zhan ( EBZ ) is a widely used donor of blast resistance in South China , with many cultivars derived from it displaying broad-spectrum resistance against blast Mapping in a set of recombinant inbred lines bred from the cross between EBZ and the highly blast-susceptible cultivar Liangjiangxintuanheigu ( LTH ) identified in EBZ a blast resistance gene on each of chromosomes 1 ( Pish ) , 6 ( Pi2/Pi9 ) and 12 ( Pita/Pita-2 ) . The resistance spectrum and race specificity of the allele at Pi2/Pi9 were both different from those present in other known Pi2/Pi9 carriers . Fine-scale mapping based on a large number of susceptible EBZ x LTH F ( 2 ) and EBZ x LTH BC ( 1 ) F ( 2 ) segregants placed the gene within a 53-kb segment , which includes Pi2/Pi9 . Sequence comparisons of the LRR motifs of the four functional NBS-LRR genes within Pi2/Pi9 revealed that the EBZ allele is distinct from other known Pi2/Pi9 alleles . As a result , the gene has been given the designation Pi50 ( t ) .
Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: As a result , the gene has been given the designation Pi50 ( t ) .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: Fructose 1 , 6-bisphosphate aldolase activity in leaves of a rice mutant selected for enhanced lysine .
Author: Schaeffer GW Sharpe FT Sicher RC .
Journal: Phytochemistry Citation: V : 46 ( 8 ) P : 1335-8 Year: 1997 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub9419899 Accession (PMID): 9419899
Abstract: Unknown proteins isolated from mutant it issues of rice ( Oryza sativa L ) recovered from inhibitor selections were subsequently peptide microsequenced . Database searches putatively identified one peptide as fructose 1 , 6-bisphosphate aldolase ( EC 4 . 1 . 2 . 13 ) . Tissues of mutant rice , PI564784 , and wild type ( cv Calrose 76 ) it issues were evaluated for aldolase activity . Total enzyme activities were slightly lower in the mutant than the control but the differences were not significant . Although the mutant phenotype is for enhanced lysine and protein , we ascribe the small aldolase differences to physiological adjustments , rather than to DNA modifications of the aldolase gene ( s ) . Homologies of rice peptides with aldolases from a range of species , as well as rice cell culture expressed sequence tags ( ESTs ) are presented . Some amino acids sequences are highly conserved . The mutant phenotype expressing stress proteins is not likely to be defined by a change in rice aldolases .
Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Tissues of mutant rice , PI564784 , and wild type ( cv Calrose 76 ) it issues were evaluated for aldolase activity .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: Genetic and physical mapping of Pi5 ( t ) , a locus associated with broad-spectrum resistance to rice blast
Author: Jeon JS Chen D Yi GH Wang GL Ronald PC .
Journal: Mol . Genet . Genomics Citation: V : 269 ( 2 ) P : 280-9 Year: 2003 Type: ARTICLE
Literature: oryza Field: title Doc ID: pub12756540 Accession (PMID): 12756540
Abstract: To gain an understanding of the molecular basis for resistance to rice blast ( Magnaporthe grisea ) , we have initiated a project to clone Pi5 ( t ) , a locus associated with broad-spectrum resistance to diverse blast isolates . AFLP-derived markers linked to Pi5 ( t ) -mediated resistance were isolated using bulked segregant analysis of F ( 2 ) populations generated by crossing three recombinant inbred lines ( RILs ) , RIL125 , RIL249 , and RIL260 with the susceptible line CO39 . The most tightly linked AFLP marker , S04G03 , was positioned on chromosome 9 of the fingerprint-based physical map of Nipponbare , a well-characterized rice genotype . Flanking BAC-based Nipponbare markers were generated for saturation mapping using four populations , the three initial RILs and an additional one derived from a cross between M202 and RIL260 . A BIBAC ( binary BAC ) library was constructed from RIL260 . Using these resources Pi5 ( t ) was mapped to a 170-kb interval , and a contiguous set of BIBAC clones spanning this region was constructed . It had previously been suggested that Pi3 ( t ) and Pi5 ( t ) might be allelic , due to their identical resistance spectrum and tight linkage . We therefore compared genomic regions for lines containing Pi3 ( t ) using the Pi5 ( t ) -linked markers . DNA gel-blot analyses indicated that the region around Pi3 ( t ) is identical to that of Pi5 ( t ) , suggesting that Pi3 ( t ) and Pi5 ( t ) are the same resistance gene .
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[ Sen. 1, subscore: 1.00 ]: Genetic and physical mapping of Pi5 ( t ) , a locus associated with broad-spectrum resistance to rice blast
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Title: Use of Pi5 ( t ) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea .
Author: Yi G Lee SK Hong YK Cho YC Nam MH Kim SC Han SS Wang GL Hahn TR Ronald PC Jeon JS .
Journal: Theor . Appl . Genet . Citation: V : 109 ( 5 ) P : 978-85 Year: 2004 Type: ARTICLE
Literature: oryza Field: title Doc ID: pub15141293 Accession (PMID): 15141293
Abstract: Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for marker-assisted selection ( MAS ) . The rice Pi5 ( t ) locus confers broad-spectrum resistance to Magnaporthe grisea , the causal agent of rice blast disease . It has been hypothesized that the Pi5 ( t ) locus carries the same gene as that encoded by the Pi3 ( t ) and Pii ( t ) loci . We developed three PCR-based dominant markers ( JJ80-T3 , JJ81-T3 , and JJ113-T3 ) from three previously identified BIBAC clones-JJ80 , JJ81 , and JJ113-that are linked to the Pi5 ( t ) locus . PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5 ( t ) , Pi3 ( t ) , and Pii ( t ) . PCR and DNA gel-blot analysis of candidate resistance lines using JJ80-T3 , JJ81-T3 , and JJ113-T3 indicated that Tetep is the likely donor of Pi5 ( t ) . Of the 184 rice varieties tested , 34 carried the JJ80-T3- , JJ81-T3- , and JJ113-T3-specific bands . Disease evaluation of those 34 varieties revealed that all conferred resistance to PO6-6 . The genomic structure of three of these resistant varieties ( ie , IR72 , Taebaeg , Jahyangdo ) is most similar to that of Pi5 ( t ) . Our results demonstrate the usefulness of the JJ80-T3 , JJ81-T3 , and JJ113-T3 markers for MAS for M grisea resistance .
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[ Sen. 1, subscore: 1.00 ]: Use of Pi5 ( t ) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea .
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Title: Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes .
Author: Lee SK Song MY Seo YS Kim HK Ko S Cao PJ Suh JP Yi G Roh JH Lee S An G Hahn TR Wang GL Ronald P Jeon JS
Journal: Genetics Citation: V : 181 P : 1627-38 Year: 2009 Type: In-Process
Literature: oryza Field: title Doc ID: pub19153255 Accession (PMID): 19153255
Abstract: Rice blast , caused by the fungus Magnaporthe oryzae , is one of the most devastating diseases of rice . To understand the molecular basis of Pi5-mediated resistance to M oryzae , we cloned the resistance ( R ) gene at this locus using a map-based cloning strategy . Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50 , a susceptible rice cultivar , and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval Sequence analysis of this genomic region identified two candidate genes , Pi5-1 and Pi5-2 , which encode proteins carrying three motifs characteristic of R genes : an N-terminal coiled-coil ( CC ) motif , a nucleotide-binding ( NB ) domain , and a leucine-rich repeat ( LRR ) motif . In genetic transformation experiments of a susceptible rice cultivar , neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M oryzae . In contrast , transgenic rice plants expressing both of these genes , generated by crossing transgenic lines carrying each gene individually , conferred Pi5-mediated resistance to M oryzae . Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge , whereas the Pi5-2 gene is constitutively expressed . These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M oryzae .
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[ Sen. 1, subscore: 1.00 ]: Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes .
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Title: The single functional blast resistance gene Pi54 activates a complex defence mechanism in rice .
Author: Gupta SK Rai AK Kanwar SS Chand D Singh NK Sharma TR
Journal: J Exp Bot Citation: V : 63 P : 757-72 Year: 2012 Type: In-Data-Review
Literature: oryza Field: title Doc ID: pub22058403 Accession (PMID): 22058403
Abstract: The Pi54 gene ( Pi-k ( h ) ) confers a high degree of resistance to diverse strains of the fungus Magnaporthe oryzae . In order to understand the genome-wide co-expression of genes in the transgenic rice plant Taipei 309 ( TP ) containing the Pi54 gene , microarray analysis was performed at 72 h post-inoculation of the M oryzae strain PLP-1 . A total of 1154 differentially expressing genes were identified in TP-Pi54 plants . Of these , 587 were up-regulated , whereas 567 genes were found to be down-regulated . 107 genes were found that were exclusively up-regulated and 58 genes that were down regulated in the case of TP-Pi54 . Various defence response genes , such as callose , laccase , PAL , and peroxidase , and genes related to transcription factors like NAC6 , Dof zinc finger , MAD box , bZIP , and WRKY were found to be up-regulated in the transgenic line . The enzymatic activities of six plant defence response enzymes , such as peroxidase , polyphenol oxidase , phenylalanine ammonia lyase , beta-glucosidase , beta-1 , 3-glucanase , and chitinase , were found to be significantly high in TP-Pi54 at different stages of inoculation by M oryzae . The total phenol content also increased significantly in resistant transgenic plants after pathogen inoculation . This study suggests the activation of defence response and transcription factor-related genes and a higher expression of key enzymes involved in the defence response pathway in the rice line TP-Pi54 , thus leading to incompatible host-pathogen interaction .
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[ Sen. 1, subscore: 1.00 ]: The single functional blast resistance gene Pi54 activates a complex defence mechanism in rice .
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Title: The identification of Pi50 ( t ) , a new member of the rice blast resistance Pi2/Pi9 multigene family .
Author: Zhu X Chen S Yang J Zhou S Zeng L Han J Su J Wang L Pan Q
Journal: Theor Appl Genet Citation: V : 124 P : 1295-304 Year: 2012 Type: In-Process
Literature: oryza Field: title Doc ID: pub22270148 Accession (PMID): 22270148
Abstract: The deployment of broad-spectrum resistance genes is the most effective and economic means of controlling blast in rice . The cultivar Er-Ba-Zhan ( EBZ ) is a widely used donor of blast resistance in South China , with many cultivars derived from it displaying broad-spectrum resistance against blast Mapping in a set of recombinant inbred lines bred from the cross between EBZ and the highly blast-susceptible cultivar Liangjiangxintuanheigu ( LTH ) identified in EBZ a blast resistance gene on each of chromosomes 1 ( Pish ) , 6 ( Pi2/Pi9 ) and 12 ( Pita/Pita-2 ) . The resistance spectrum and race specificity of the allele at Pi2/Pi9 were both different from those present in other known Pi2/Pi9 carriers . Fine-scale mapping based on a large number of susceptible EBZ x LTH F ( 2 ) and EBZ x LTH BC ( 1 ) F ( 2 ) segregants placed the gene within a 53-kb segment , which includes Pi2/Pi9 . Sequence comparisons of the LRR motifs of the four functional NBS-LRR genes within Pi2/Pi9 revealed that the EBZ allele is distinct from other known Pi2/Pi9 alleles . As a result , the gene has been given the designation Pi50 ( t ) .
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[ Sen. 1, subscore: 1.00 ]: The identification of Pi50 ( t ) , a new member of the rice blast resistance Pi2/Pi9 multigene family .
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Title: Identification of the novel recessive gene pi55 ( t ) conferring resistance to Magnaporthe oryzae .
Author: He X Liu X Wang L Wang L Lin F Cheng Y Chen Z Liao Y Pan Q
Journal: Sci China Life Sci Citation: V : 55 P : 141-9 Year: 2012 Type: MEDLINE
Literature: oryza Field: title Doc ID: pub22415685 Accession (PMID): 22415685
Abstract: The elite rice cultivar Yuejingsimiao 2 ( YJ2 ) is characterized by a high level of grain quality and yield , and resistance against Magnaporthe oryzae . YJ2 showed 100% resistance to four fungal populations collected from Guangdong , Sichuan , Liaoning , and Heilongjiang Provinces , which is a higher frequency than that shown by the well-known resistance ( R ) gene donor cultivars such as Sanhuangzhan 2 and 28zhan . Segregation analysis for resistance with F ( 2 ) and F ( 4 ) populations indicated the resistance of YJ2 was controlled by multiple genes that are dominant or recessive . The putative R genes of YJ2 were roughly tagged by SSR markers , located on chromosomes 2 , 6 , 8 , and 12 , in a bulked-segregant analysis using genome-wide selected SSR markers with F ( 4 ) lines that segregated into 3 resistant ( R ) : 1 susceptible ( S ) or 1R : 3S . The recessive R gene on chromosome 8 was further mapped to an interval approximately 1 . 9 cM/152 kb in length by linkage analysis with genomic position-ready markers in the mapping population derived from an F ( 4 ) line that segregated into 1R : 3S . Given that no major R gene was mapped to this interval , the novel R gene was designated as pi55 ( t ) . Out of 26 candidate genes predicted in the region based on the reference genomic sequence of the cultivar Nipponbare , two genes that encode a leucine-rich repeat-containing protein and heavy-metal-associated domain-containing protein , respectively , were suggested as the most likely candidates for pi55 ( t ) .
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[ Sen. 1, subscore: 1.00 ]: Identification of the novel recessive gene pi55 ( t ) conferring resistance to Magnaporthe oryzae .
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Title: A novel blast resistance gene , Pi54rh cloned from wild species of rice , Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae .
Author: Das A Soubam D Singh PK Thakur S Singh NK Sharma TR
Journal: Funct Integr Genomics Citation: V : 12 P : 215-28 Year: 2012 Type: In-Process
Literature: oryza Field: title Doc ID: pub22592658 Accession (PMID): 22592658
Abstract: The dominant rice blast resistance gene , Pi54 confers resistance to Magnaporthe oryzae in different parts of India . In our effort to identify more effective forms of this gene , we isolated an orthologue of Pi54 named as Pi54rh from the blast-resistant wild species of rice , Oryza rhizomatis , using allele mining approach and validated by complementation . The Pi54rh belongs to CC-NBS-LRR family of disease resistance genes with a unique Zinc finger ( C ( 3 ) H type ) domain . The 1 , 447 bp Pi54rh transcript comprises of 101 bp 5-UTR , 1 , 083 bp coding region and 263 bp 3-UTR , driven by pathogen inducible promoter . We showed the extracellular localization of Pi54rh protein and the presence of glycosylation , myristoylation and phosphorylation sites which implicates its role in signal transduction process . This is in contrast to other blast resistance genes that are predicted to be intracellular NBS-LRR-type resistance proteins . The Pi54rh was found to express constitutively at basal level in the leaves , but upregulates 3 . 8-fold at 96 h post-inoculation with the pathogen . Functional validation of cloned Pi54rh gene using complementation test showed high degree of resistance to seven isolates of M oryzae collected from different geographical locations of India . In this study , for the first time , we demonstrated that a rice blast resistance gene Pi54rh cloned from wild species of rice provides broad spectrum resistance to M oryzae hence can be used in rice improvement breeding programme .
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[ Sen. 1, subscore: 1.00 ]: A novel blast resistance gene , Pi54rh cloned from wild species of rice , Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae .
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Title: Molecular mapping of the blast resistance genes Pi2-1 and Pi51 ( t ) in the durably resistant rice Tianjingyeshengdao .
Author: Wang Y Wang D Deng X Liu J Sun P Liu Y Huang H Jiang N Kang H Ning Y Wang Z Xiao Y Liu X Liu E Dai L Wang GL
Journal: Phytopathology Citation: V : 102 P : 779-86 Year: 2012 Type: In-Process
Literature: oryza Field: title Doc ID: pub22779744 Accession (PMID): 22779744
Abstract: Tianjingyeshengdao ( TY ) is a rice cultivar with durable resistance to populations of Magnaporthe oryzae ( the causal agent of blast ) in China . To understand the genetic basis of its resistance to blast , we developed a population of recombinant inbred lines from a cross between TY and the highly susceptible CO39 for gene mapping analysis . In total , 22 quantitative trait loci ( QTLs ) controlling rice blast resistance were identified on chromosomes 1 , 3 , 4 , 5 , 6 , 9 , 11 , and 12 from the evaluation of four disease parameters in both greenhouse and blast nursery conditions . Among these QTLs , 19 were contributed by TY and three by CO39 . Two QTL clusters on chromosome 6 and 12 were named Pi2-1 and Pi51 ( t ) , respectively . Pi2-1 was detected under both growth chamber and natural blast nursery conditions , and explained 31 . 24 to 59 . 73% of the phenotypic variation . Pi51 ( t ) was only detected in the natural blast nursery and explained 3 . 67 to 10 . 37% of the phenotypic variation . Our results demonstrate that the durable resistance in TY is controlled by two major and seven minor genes . Identification of the markers linked to both Pi2-1 and Pi51 ( t ) in this study should be useful for marker-aided selection in rice breeding programs as well as for molecular cloning of the identified resistance genes .
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[ Sen. 1, subscore: 1.00 ]: Molecular mapping of the blast resistance genes Pi2-1 and Pi51 ( t ) in the durably resistant rice Tianjingyeshengdao .
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