Score: 3.00 |
|---|
| Title: Functional analysis of six drought-inducible promoters in transgenic rice plants throughout all stages of plant growth .
| | Author: Yi N Kim YS Jeong MH Oh SJ Jeong JS Park SH Jung H Choi YD Kim JK | | Journal: Planta Citation: V : 232 P : 743-54 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20567981 Accession (PMID): 20567981 | | Abstract: There are few efficient promoters for use with stress-inducible gene expression in plants , and in particular for monocotyledonous crops .
Here , we report the identification of six genes , Rab21 , Wsi18 , Lea3 , Uge1 , Dip1 , and R1G1B that were induced by drought stress in rice microarray experiments .
Gene promoters were linked to the gfp reporter and their activities were analyzed in transgenic rice plants throughout all stages of plant growth , from dry seeds to vegetative it issues to flowers , both before and after drought treatments .
In fold induction levels , Rab21 and Wsi18 promoters ranged from 65 and 36-fold in leaves to 1 , 355 and 492-fold in flowers , respectively , whereas Lea3 and Uge1 were higher in leaves , but lower in roots and flowers , as compared with Rab21 and Wsi18 .
Dip1 and R1G1B promoters had higher basal levels of activity under normal growth conditions in all it issues , resulting in smaller fold-induction levels than those of the others .
In drought treatment time course , activities of Dip1 and R1G1B promoters rapidly increased , peaked at 2 h , and remained constant until 8 h , while that of Lea3 slowly yet steadily increased until 8 h .
Interestingly , Rab21 activity increased rapidly and steadily in response to drought stress until expression peaked at 8 h .
Thus , we have isolated and characterized six rice promoters that are all distinct in fold induction , it issue specificity , and induction kinetics under drought conditions , providing a variety of drought-inducible promoters for crop biotechnology .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Here , we report the identification of six genes , Rab21 , Wsi18 , Lea3 , Uge1 , Dip1 , and R1G1B that were induced by drought stress in rice microarray experiments .
[ Sen. 4, subscore: 1.00 ]: In fold induction levels , Rab21 and Wsi18 promoters ranged from 65 and 36-fold in leaves to 1 , 355 and 492-fold in flowers , respectively , whereas Lea3 and Uge1 were higher in leaves , but lower in roots and flowers , as compared with Rab21 and Wsi18 .
[ Sen. 6, subscore: 1.00 ]: In drought treatment time course , activities of Dip1 and R1G1B promoters rapidly increased , peaked at 2 h , and remained constant until 8 h , while that of Lea3 slowly yet steadily increased until 8 h .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses ( Poaceae ) .
| | Author: Yu L Wu X Tang X Yan B | | Journal: Genet Mol Res Citation: V : 9 P : 1321-5 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20623457 Accession (PMID): 20623457 | | Abstract: cDNA fragments of lea3 genes with a high GC content ( from 68 to 77% ) were found in several Poaceae , including Sorghum vulgare , Saccharum officinarum , Oryza officinalis , Oryza meyeriana , Ampelocalamus calcareus , Cynodon dactylon , and Zizania latifoli .
They were successfully isolated by means of optimal experimental parameters , which included dimethyl sulfoxide as additive and degenerate primers "AGETKAS" and "AGKDKTG" , and their sequences were analyzed .
Compared to the method of isolating genes by screening of a cDNA library using abscisic acid and other stress-responsive cDNA clones , which is time-consuming and costly , this method is relatively easy and inexpensive .
Using this new method , many new homologue lea3 genes were rapidly determined .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: cDNA fragments of lea3 genes with a high GC content ( from 68 to 77% ) were found in several Poaceae , including Sorghum vulgare , Saccharum officinarum , Oryza officinalis , Oryza meyeriana , Ampelocalamus calcareus , Cynodon dactylon , and Zizania latifoli .
[ Sen. 4, subscore: 1.00 ]: Using this new method , many new homologue lea3 genes were rapidly determined .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses ( Poaceae ) .
| | Author: Yu L Wu X Tang X Yan B | | Journal: Genet Mol Res Citation: V : 9 P : 1321-5 Year: 2010 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub20623457 Accession (PMID): 20623457 | | Abstract: cDNA fragments of lea3 genes with a high GC content ( from 68 to 77% ) were found in several Poaceae , including Sorghum vulgare , Saccharum officinarum , Oryza officinalis , Oryza meyeriana , Ampelocalamus calcareus , Cynodon dactylon , and Zizania latifoli .
They were successfully isolated by means of optimal experimental parameters , which included dimethyl sulfoxide as additive and degenerate primers "AGETKAS" and "AGKDKTG" , and their sequences were analyzed .
Compared to the method of isolating genes by screening of a cDNA library using abscisic acid and other stress-responsive cDNA clones , which is time-consuming and costly , this method is relatively easy and inexpensive .
Using this new method , many new homologue lea3 genes were rapidly determined .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses ( Poaceae ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
