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7 matches found in 3 documents. Search time: 0.002 seconds.
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Score: 4.00
Title: An AFLP marker that differentiates biotypes of the Asian rice gall midge ( Orseolia oryzae , Wood-Mason ) is sex-linked and also linked to avirulence .
Author: Behura SK Nair S Sahu SC Mohan M
Journal: Mol . Gen . Genet . Citation: V : 263 ( 2 ) P : 328-34 Year: 2000 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub10778752 Accession (PMID): 10778752
Abstract: In an attempt to identify a specific marker for biotype 2 of the Asian rice gall midge ( Orseolia oryzae , Wood-Mason ) , we used AFLP ( amplified fragment length polymorphism ) fingerprinting . We identified an AFLP marker that is specifically amplified in biotypes 1 , 2 and 5 of the rice gall midge , but not in biotype 4 . Biotypes 1 , 2 and 5 are avirulent to hosts bearing the Gm2 resistance gene ( found in rice variety Phalguna ) , whereas biotype 4 is virulent to Gm2 . Based on the sequence of this AFLP marker , SCAR ( sequence characterized amplified region ) primers were designed and used in combination with previously developed SCAR primers to distinguish effectively all five biotypes in a multiplex PCR-based assay . The inheritance pattern of this marker in the progenies of inter-biotype crosses between biotypes 1 , 2 and 4 shows that the marker can be amplified by PCR from all F1 females , irrespective of the biotype status of their parents . However , the marker is present only in those male progenies whose mother was of a Gm2 avirulent biotype . The specific amplification of this marker in the avirulent biotypes and its pattern of inheritance show that avirulence with respect to carriers of the Gm2 gene in rice gall midge is sex-linked .
Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: Biotypes 1 , 2 and 5 are avirulent to hosts bearing the Gm2 resistance gene ( found in rice variety Phalguna ) , whereas biotype 4 is virulent to Gm2 .
[ Sen. 6, subscore: 1.00 ]: However , the marker is present only in those male progenies whose mother was of a Gm2 avirulent biotype .
[ Sen. 7, subscore: 1.00 ]: The specific amplification of this marker in the avirulent biotypes and its pattern of inheritance show that avirulence with respect to carriers of the Gm2 gene in rice gall midge is sex-linked .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 4.00
Title: Identification and mapping of an AFLP marker linked to Gm7 , a gall midge resistance gene and its conversion to a SCAR marker for its utility in marker aided selection in rice .
Author: Sardesai N Kumar A Rajyashri R Nair S Mohan M
Journal: Citation: V : 105 ( 5 ) P : 691-698 Year: 2002 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub12582482 Accession (PMID): 12582482
Abstract: We have identified an AFLP marker SA598 that is linked to Gm7 , a gene conferring resistance to biotypes 1 , 2 and 4 of the gall midge ( Orseolia oryzae ) , a major dipteran pest of rice . A set of PCR primers specific to an RFLP marker , previously identified to be linked to another gall midge resistance gene Gm2 , also amplified a 1 . 5-kb ( F8LB ) fragment that is linked to Gm7 . Gm7 is a dominant gene and non-allelic to Gm2 . Hybridization experiments with clones from a YAC library of Nipponbare , a japonica variety , a BAC library of IR-BB21 , an indica variety , and cosmid clones encompassing Gm2 from Phalguna , an indica variety , with F8LB and SA598 as probes , revealed that Gm7 is tightly linked to Gm2 and is located on chromosome 4 of rice . SA598 was sequenced and the sequence information was used to design sequence-characterized amplified region ( SCAR ) primers . The potential use of these SCAR primers in marker-aided selection of Gm7 in a rice breeding program has been demonstrated .
Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Hybridization experiments with clones from a YAC library of Nipponbare , a japonica variety , a BAC library of IR-BB21 , an indica variety , and cosmid clones encompassing Gm2 from Phalguna , an indica variety , with F8LB and SA598 as probes , revealed that Gm7 is tightly linked to Gm2 and is located on chromosome 4 of rice .
[ Sen. 2, subscore: 1.00 ]: A set of PCR primers specific to an RFLP marker , previously identified to be linked to another gall midge resistance gene Gm2 , also amplified a 1 . 5-kb ( F8LB ) fragment that is linked to Gm7 .
[ Sen. 3, subscore: 1.00 ]: Gm7 is a dominant gene and non-allelic to Gm2 .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: Tagging and mapping of a rice gall midge resistance gene , Gm8 , and development of SCARs for use in marker-aided selection and gene pyramiding .
Author: Jain A Ariyadasa R Kumar A Srivastava MN Mohan M Nair S
Journal: Theor . Appl . Genet . Citation: V : 109 ( 7 ) P : 1377-84 Year: 2004 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub15322754 Accession (PMID): 15322754
Abstract: Using amplified fragment length polymorphisms ( AFLPs ) and random amplified polymorphic DNAs ( RAPDs ) , we have tagged and mapped Gm8 , a gene conferring resistance to the rice gall midge ( Orseolia oryzae ) , a major insect pest of rice , onto rice chromosome 8 . Using AFLPs , two fragments , AR257 and AS168 , were identified that were linked to the resistant and susceptible phenotypes , respectively . Another resistant phenotype-specific marker , AP19 ( 587 ) , was also identified using RAPDs . SCAR primers based on the sequence of the fragments AR257 and AS168 failed to reveal polymorphism between the resistant and the susceptible parents . However , PCR using primers based on the regions flanking AR257 revealed polymorphism that was phenotype-specific . In contrast , PCR carried out using primers flanking the susceptible phenotype-associated fragment AS168 produced a monomorphic fragment . Restriction digestion of these monomorphic fragments revealed polymorphism between the susceptible and resistant parents . Nucleotide BLAST searches revealed that the three fragments show strong homology to rice PAC and BAC clones that formed a contig representing the short arm of chromosome 8 . PCR amplification using the above-mentioned primers on a larger population , derived from a cross between two indica rice varieties , Jhitpiti ( resistant parent ) and TN1 ( susceptible parent ) , showed that there is a tight linkage between the markers and the Gm8 locus . These markers , therefore , have potential for use in marker-aided selection and pyramiding of Gm8 along with other previously tagged gall midge resistance genes [ Gm2 , Gm4 ( t ) , and Gm7 ] .
Matching Sentences:
[ Sen. 10, subscore: 1.00 ]: These markers , therefore , have potential for use in marker-aided selection and pyramiding of Gm8 along with other previously tagged gall midge resistance genes [ Gm2 , Gm4 ( t ) , and Gm7 ] .
Supplemental links/files: reference in endnote online text related articles pubmed citation
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