13 matches found in 6 documents. Search time: 0.042 seconds. |
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Score: 4.00 | Title: OsDREB genes in rice , Oryza sativa L , encode transcription activators that function in drought- , high-salt and cold-responsive gene expression .
| Author: Dubouzet JG Sakuma Y Ito Y Kasuga M Dubouzet EG Miura S Seki M Shinozaki K Yamaguchi-Shinozaki K | Journal: Plant J Citation: V : 33 ( 4 ) P : 751-63 Year: 2003 | Literature: oryza Field: abstract Doc ID: pub12609047 Accession (PMID): 12609047 | Abstract: The transcription factors DREBs/CBFs specifically interact with the dehydration-responsive element/C-repeat ( DRE/CRT ) cis-acting element ( core motif : G/ACCGAC ) and control the expression of many stress-inducible genes in Arabidopsis .
In rice , we isolated five cDNAs for DREB homologs : OsDREB1A , OsDREB1B , OsDREB1C , OsDREB1D , and OsDREB2A .
Expression of OsDREB1A and OsDREB1B was induced by cold , whereas expression of OsDREB2A was induced by dehydration and high-salt stresses .
The OsDREB1A and OsDREB2A proteins specifically bound to DRE and activated the transcription of the GUS reporter gene driven by DRE in rice protoplasts .
Over-expression of OsDREB1A in transgenic Arabidopsis induced over-expression of target stress-inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought , high-salt , and freezing stresses .
This indicated that OsDREB1A has functional similarity to DREB1A .
However , in microarray and RNA blot analyses , some stress-inducible target genes of the DREB1A proteins that have only ACCGAC as DRE were not over-expressed in the OsDREB1A transgenic Arabidopsis .
The OsDREB1A protein bound to GCCGAC more preferentially than to ACCGAC whereas the DREB1A proteins bound to both GCCGAC and ACCGAC efficiently .
The structures of DREB1-type ERF/AP2 domains in monocots are closely related to each other as compared with that in the dicots .
OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought , high-salt , and/or cold stresses . | Matching Sentences: [ Sen. 5, subscore: 1.00 ]: Over-expression of OsDREB1A in transgenic Arabidopsis induced over-expression of target stress-inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought , high-salt , and freezing stresses . [ Sen. 6, subscore: 1.00 ]: This indicated that OsDREB1A has functional similarity to DREB1A . [ Sen. 7, subscore: 1.00 ]: However , in microarray and RNA blot analyses , some stress-inducible target genes of the DREB1A proteins that have only ACCGAC as DRE were not over-expressed in the OsDREB1A transgenic Arabidopsis . [ Sen. 8, subscore: 1.00 ]: The OsDREB1A protein bound to GCCGAC more preferentially than to ACCGAC whereas the DREB1A proteins bound to both GCCGAC and ACCGAC efficiently .
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Score: 3.00 | Title: Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions .
| Author: Pellegrineschi A Reynolds M Pacheco M Brito RM Almeraya R Yamaguchi-Shinozaki K Hoisington D | Journal: Genome Citation: V : 47 ( 3 ) P : 493-500 Year: 2004 | Literature: oryza Field: abstract Doc ID: pub15190366 Accession (PMID): 15190366 | Abstract: One of the major environmental factors limiting plant productivity is lack of water .
This is especially true for the major cereals maize , rice , and wheat , which demonstrate a range of susceptibility to moisture deficit .
Although conventional breeding and marker-assisted selection are being used to develop varieties more tolerant to water stress , these methods are time and resource consuming and germplasm dependent .
Genetic engineering is attractive because of its potential to improve abiotic stress tolerance more rapidly .
Transcription factors have been shown to produce multiple phenotypic alterations , many of which are involved in stress responses .
DREB1A , a transcription factor that recognizes dehydration response elements , has been shown in Arabidopsis thaliana to play a crucial role in promoting the expression of drought-tolerance genes .
In our efforts to enhance drought tolerance in wheat , the A thaliana DREB1A gene was placed under control of a stress-inducible promoter from the rd29A gene and transferred via biolistic transformation into bread wheat .
Plants expressing the DREB1A gene demonstrated substantial resistance to water stress in comparison with checks under experimental greenhouse conditions , manifested by a 10-day delay in wilting when water was withheld .
| Matching Sentences: [ Sen. 6, subscore: 1.00 ]: DREB1A , a transcription factor that recognizes dehydration response elements , has been shown in Arabidopsis thaliana to play a crucial role in promoting the expression of drought-tolerance genes . [ Sen. 7, subscore: 1.00 ]: In our efforts to enhance drought tolerance in wheat , the A thaliana DREB1A gene was placed under control of a stress-inducible promoter from the rd29A gene and transferred via biolistic transformation into bread wheat . [ Sen. 8, subscore: 1.00 ]: Plants expressing the DREB1A gene demonstrated substantial resistance to water stress in comparison with checks under experimental greenhouse conditions , manifested by a 10-day delay in wilting when water was withheld .
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Score: 3.00 | Title: Overexpression of Arabidopsis and rice stress genes inducible transcription factor confers drought and salinity tolerance to rice .
| Author: Datta K Baisakh N Ganguly M Krishnan S Yamaguchi Shinozaki K Datta SK | Journal: Plant Biotechnol J Citation: V : 10 P : 579-86 Year: 2012 | Literature: oryza Field: abstract Doc ID: pub22385556 Accession (PMID): 22385556 | Abstract: Rice yield is greatly affected by environmental stresses such as drought and salinity .
In response to the challenge of producing rice plants tolerant to these stresses , we introduced cDNA encoding the transcription factors DREB1A and DREB1B under the control of the stress inducible rd29 promoter .
Two different indica rice cultivars were used , BR29 , an improved commercially cultivated variety from Bangladesh and IR68899B , an IRRI bred maintainer line for hybrid rice .
Agrobacterium mediated transformation of BR29 was done independently with DREB1A isolated from rice and Arabidopsis and DREB1B isolated from rice , whereas biolistic transformation was done with rice DREB1B in the case of IR68899B .
Initial genetic integration was confirmed by PCR and Southern blot analysis .
Salinity tolerance was assayed in very young seedlings .
Drought stress tests were found to be more reliable when they were carried out at the pre-flowering booting stage .
RNA gel blot analysis as well as quantitative PCR analysis was performed to estimate the transcription level under stressed and unstressed conditions .
Agronomic performance studies were done with stressed and unstressed plants to compare the yield losses due to dehydration and salt loading stresses .
Noticeably enhanced tolerance to dehydration was observed in the plants transformed with DREB1A isolated from Arabidopsis while DREB1B was found to be more effective for salt tolerance .
| Matching Sentences: [ Sen. 2, subscore: 1.00 ]: In response to the challenge of producing rice plants tolerant to these stresses , we introduced cDNA encoding the transcription factors DREB1A and DREB1B under the control of the stress inducible rd29 promoter . [ Sen. 4, subscore: 1.00 ]: Agrobacterium mediated transformation of BR29 was done independently with DREB1A isolated from rice and Arabidopsis and DREB1B isolated from rice , whereas biolistic transformation was done with rice DREB1B in the case of IR68899B . [ Sen. 10, subscore: 1.00 ]: Noticeably enhanced tolerance to dehydration was observed in the plants transformed with DREB1A isolated from Arabidopsis while DREB1B was found to be more effective for salt tolerance .
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Score: 1.00 | Title: Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays L | Author: Qin F Sakuma Y Li J Liu Q Li YQ Shinozaki K Yamaguchi-Shinozaki K | Journal: Plant Cell Physiol .
Citation: V : 45 ( 8 ) P : 1042-52 Year: 2004 | Literature: oryza Field: abstract Doc ID: pub15356330 Accession (PMID): 15356330 | Abstract: The transcription factors DREB1s/CBFs specifically interact with the DRE/CRT cis-acting element ( core motif : G/ACCGAC ) and control the expression of many stress-inducible genes in Arabidopsis .
We isolated a cDNA for a DREB1/CBF homolog , ZmDREB1A in maize using a yeast one-hybrid system .
The ZmDREB1A proteins specifically bound to DRE and the highly conserved valine at the 14th residue in the ERF/AP2 DNA binding domain was a key to determining the specific interaction between this protein and the DRE sequence .
Expression of ZmDREB1A was induced by cold stress and slightly increased by high-salinity stress .
This gene was also transiently expressed by mechanical attack .
ZmDREB1A activated the transcription of the GUS reporter gene driven by DRE in rice protoplasts .
Overexpression of ZmDREB1A in transgenic Arabidopsis induced overexpression of target stress-inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought and freezing stresses .
This indicated that ZmDREB1A has functional similarity to DREB1s/CBFs in Arabidopsis .
The structure of the ERF/AP2 domain of ZmDREB1A in maize is closely related to DREB1-type ERF/AP2 domains in the monocots as compared with that in the dicots .
ZmDREB1A is suggested to be potentially useful for producing transgenic plants that is tolerant to drought , high-salinity and/or cold stresses .
| Matching Sentences: [ Sen. 7, subscore: 1.00 ]: Overexpression of ZmDREB1A in transgenic Arabidopsis induced overexpression of target stress-inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought and freezing stresses .
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Score: 1.00 | Title: Generation of chimeric repressors that confer salt tolerance in Arabidopsis and rice .
| Author: Mito T Seki M Shinozaki K Ohme-Takagi M Matsui K | Journal: Plant Biotechnol J Citation: V : 9 P : 736-46 Year: 2011 | Literature: oryza Field: abstract Doc ID: pub21114612 Accession (PMID): 21114612 | Abstract: We show here that transgenic Arabidopsis plants that expressed chimeric repressors derived from the AtMYB102 , ANAC047 , HRS1 , ZAT6 and AtERF5 transcription factors were tolerant to treatment with 400 mm NaCl , which was lethal to wild-type plants .
The transgenic plants grew well , without any apparent differences from the wild-type plants under normal growth condition .
The transgenic lines expressing the AtMYB102 , ANAC047 and HRS1 chimeric repressors germinated in the presence of 225 mm NaCl , while those expressing the ZAT6 and AtERF5 did not .
However , the latter lines were tolerant to osmotic stress and germinated in the presence of 600 mm mannitol , suggesting a link between responses to salt and osmotic stress .
Expression of the AtMYB102 , ANAC047 , ZAT6 and AtERF5 genes was induced by salt treatment , while that of HRS1 was repressed .
HRS1 has transcriptional repressive activity and appears to suppress the expression of factors that negatively regulate salt tolerance .
Microarray analysis revealed that the levels of expression of DREB1A , DREB2B and several genes for ZAT transcription factors rose 10 to 100-fold in the AtMYB102 chimeric repressor line under both normal and stress conditions .
Elevated expression of DREB and ZAT related genes might be involved in the salt tolerance of the AtMYB102 chimeric repressor line .
Transgenic rice plants expressing chimeric repressors derived from Os02g0325600 and Os03g0327800 , rice homologues of HRS1 and ANAC047 , were tolerant to salinity stress demonstrated by suppression of growth inhibition and ion leakages .
Expression of a chimeric repressor provides an effective strategy for enhancing tolerance of plants to abiotic stress .
| Matching Sentences: [ Sen. 7, subscore: 1.00 ]: Microarray analysis revealed that the levels of expression of DREB1A , DREB2B and several genes for ZAT transcription factors rose 10 to 100-fold in the AtMYB102 chimeric repressor line under both normal and stress conditions .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |