Score: 8.00 |
|---|
| Title: Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins .
| | Author: Sakane I Kamataki C Takizawa Y Nakashima M Toki S Ichikawa H Ikawa S Shibata T Kurumizaka H | | Journal: Nucleic Acids Res Citation: V : 36 P : 4266-76 Year: 2008 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18583359 Accession (PMID): 18583359 | | Abstract: The DMC1 protein , a meiosis-specific DNA recombinase , catalyzes strand exchange between homologous chromosomes .
In rice , two Dmc1 genes , Dmc1A and Dmc1B , have been reported .
Although the Oryza sativa DMC1A protein has been partially characterized , however the biochemical properties of the DMC1B protein have not been defined .
In the present study , we expressed the Oryza sativa DMC1A and DMC1B proteins in bacteria and purified them .
The purified DMC1A and DMC1B proteins formed helical filaments along single-stranded DNA ( ssDNA ) and double-stranded DNA ( dsDNA ) , and promoted robust strand exchange between ssDNA and dsDNA over five thousand base pairs in the presence of RPA , as a co-factor .
The DMC1A and DMC1B proteins also promoted strand exchange in the absence of RPA with long DNA substrates containing several thousand base pairs .
In contrast , the human DMC1 protein strictly required RPA to promote strand exchange with these long DNA substrates .
The strand-exchange activity of the Oryza sativa DMC1A protein was much higher than that of the DMC1B protein .
Consistently , the DNA-binding activity of the DMC1A protein was higher than that of the DMC1B protein .
These biochemical differences between the DMC1A and DMC1B proteins may provide important insight into their functional differences during meiosis in rice .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: In rice , two Dmc1 genes , Dmc1A and Dmc1B , have been reported .
[ Sen. 3, subscore: 1.00 ]: Although the Oryza sativa DMC1A protein has been partially characterized , however the biochemical properties of the DMC1B protein have not been defined .
[ Sen. 4, subscore: 1.00 ]: In the present study , we expressed the Oryza sativa DMC1A and DMC1B proteins in bacteria and purified them .
[ Sen. 5, subscore: 1.00 ]: The purified DMC1A and DMC1B proteins formed helical filaments along single-stranded DNA ( ssDNA ) and double-stranded DNA ( dsDNA ) , and promoted robust strand exchange between ssDNA and dsDNA over five thousand base pairs in the presence of RPA , as a co-factor .
[ Sen. 6, subscore: 1.00 ]: The DMC1A and DMC1B proteins also promoted strand exchange in the absence of RPA with long DNA substrates containing several thousand base pairs .
[ Sen. 8, subscore: 1.00 ]: The strand-exchange activity of the Oryza sativa DMC1A protein was much higher than that of the DMC1B protein .
[ Sen. 9, subscore: 1.00 ]: Consistently , the DNA-binding activity of the DMC1A protein was higher than that of the DMC1B protein .
[ Sen. 10, subscore: 1.00 ]: These biochemical differences between the DMC1A and DMC1B proteins may provide important insight into their functional differences during meiosis in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins .
| | Author: Sakane I Kamataki C Takizawa Y Nakashima M Toki S Ichikawa H Ikawa S Shibata T Kurumizaka H | | Journal: Nucleic Acids Res Citation: V : 36 P : 4266-76 Year: 2008 Type: In-Process | | Literature: oryza Field: title Doc ID: pub18583359 Accession (PMID): 18583359 | | Abstract: The DMC1 protein , a meiosis-specific DNA recombinase , catalyzes strand exchange between homologous chromosomes .
In rice , two Dmc1 genes , Dmc1A and Dmc1B , have been reported .
Although the Oryza sativa DMC1A protein has been partially characterized , however the biochemical properties of the DMC1B protein have not been defined .
In the present study , we expressed the Oryza sativa DMC1A and DMC1B proteins in bacteria and purified them .
The purified DMC1A and DMC1B proteins formed helical filaments along single-stranded DNA ( ssDNA ) and double-stranded DNA ( dsDNA ) , and promoted robust strand exchange between ssDNA and dsDNA over five thousand base pairs in the presence of RPA , as a co-factor .
The DMC1A and DMC1B proteins also promoted strand exchange in the absence of RPA with long DNA substrates containing several thousand base pairs .
In contrast , the human DMC1 protein strictly required RPA to promote strand exchange with these long DNA substrates .
The strand-exchange activity of the Oryza sativa DMC1A protein was much higher than that of the DMC1B protein .
Consistently , the DNA-binding activity of the DMC1A protein was higher than that of the DMC1B protein .
These biochemical differences between the DMC1A and DMC1B proteins may provide important insight into their functional differences during meiosis in rice .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
