| 201 matches found in 91 documents. Search time: 0.602 seconds. |
|---|
|
Score: 14.00 |
|---|
| Title: d14 , a strigolactone-insensitive mutant of rice , shows an accelerated outgrowth of tillers .
| | Author: Arite T Umehara M Ishikawa S Hanada A Maekawa M Yamaguchi S Kyozuka J | | Journal: Plant Cell Physiol Citation: V : 50 P : 1416-24 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19542179 Accession (PMID): 19542179 | | Abstract: Recent studies using highly branched mutants of pea , Arabidopsis and rice have demonstrated that strigolactones , a group of terpenoid lactones , act as a new hormone class , or its biosynthetic precursors , in inhibiting shoot branching .
Here , we provide evidence that DWARF14 ( D14 ) inhibits rice tillering and may act as a new compo-nent of the strigolactone-dependent branching inhibition pathway .
The d14 mutant exhibits increased shoot branch-ing with reduced plant height like the previously characterized strigolactone-deficient and -insensitive mutants d10 and d3 , respectively .
The d10-1 d14-1 double mutant is phenotypically indistinguishable from the d10-1 and d14-1 single mutants , consistent with the idea that D10 and D14 function in the same pathway .
However , unlike with d10 , the d14 branching phenotype could not be rescued by exogenous strigolactones .
In addition , the d14 mutant contained a higher level of 2-epi-5-deoxystrigol than the wild type .
Positional cloning revealed that D14 encodes a protein of the alpha/beta-fold hydrolase superfamily , some members of which play a role in metabolism or signaling of plant hormones .
We propose that D14 functions downstream of strigolactone synthesis , as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form .
| Matching Sentences:
[ Sen. 4, subscore: 6.00 ]: The d10-1 d14-1 double mutant is phenotypically indistinguishable from the d10-1 and d14-1 single mutants , consistent with the idea that D10 and D14 function in the same pathway .
[ Sen. 3, subscore: 2.00 ]: The d14 mutant exhibits increased shoot branch-ing with reduced plant height like the previously characterized strigolactone-deficient and -insensitive mutants d10 and d3 , respectively .
[ Sen. 5, subscore: 2.00 ]: However , unlike with d10 , the d14 branching phenotype could not be rescued by exogenous strigolactones .
[ Sen. 2, subscore: 1.00 ]: Here , we provide evidence that DWARF14 ( D14 ) inhibits rice tillering and may act as a new compo-nent of the strigolactone-dependent branching inhibition pathway .
[ Sen. 6, subscore: 1.00 ]: In addition , the d14 mutant contained a higher level of 2-epi-5-deoxystrigol than the wild type .
[ Sen. 7, subscore: 1.00 ]: Positional cloning revealed that D14 encodes a protein of the alpha/beta-fold hydrolase superfamily , some members of which play a role in metabolism or signaling of plant hormones .
[ Sen. 8, subscore: 1.00 ]: We propose that D14 functions downstream of strigolactone synthesis , as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 13.00 |
|---|
| Title: Study of novel d1 alleles , defective mutants of the alpha subunit of heterotrimeric G-protein in rice .
| | Author: Oki K Inaba N Kitano H Takahashi S Fujisawa Y Kato H Iwasaki Y | | Journal: Genes Genet Syst Citation: V : 84 P : 35-42 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19420799 Accession (PMID): 19420799 | | Abstract: It has been shown that the disruption of the alpha-subunit gene of heterotorimeric G-proteins ( Galpha ) results in dwarf traits , the erection of leaves and the setting of small seeds in rice .
These mutants are called d1 .
We have studied the expression profiles of the transcripts and translation products of rice Galpha in ten alleles of d1 including five additional alleles newly identified .
By RT-PCR , the transcripts of the Galpha gene were detected in the all d1 alleles .
By western blot , the Galpha proteins were not detected in the plasma membrane fractions of the d1 alleles with the exception of d1-4 .
In d1-4 , one amino acid change in the GTP-binding box A of the Galpha protein was occurred and even in this case the Galpha protein was only just detectable in the plasma membrane fraction .
Given that the Galpha protein did not accumulate in the plasma membrane fraction in d1-8 which has a deletion of just a single amino acid in the Galpha protein , it is likely that a proper conformation of the Galpha is necessary for accumulation of Galpha protein in the plasma membrane .
Nine alleles of d1 showed a severer phenotype whilst d1-4 exhibited a mild phenotype with respect to seed size and elongation pattern of internodes .
As brassinosteroid signaling was known to be partially impaired in d1s , the sensitivity to 24-epibrassinolide ( 24-epiBL ) was compared among d1 alleles in a T65 genetic background .
Only d1-4 showed responses similar to wild type rice .
The results show that the d1-4 mutant is a mild allele in terms of the phenotype and mild hyposensitivity to the exogenously applied 24-epiBL .
| Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: By western blot , the Galpha proteins were not detected in the plasma membrane fractions of the d1 alleles with the exception of d1-4 .
[ Sen. 8, subscore: 2.00 ]: Nine alleles of d1 showed a severer phenotype whilst d1-4 exhibited a mild phenotype with respect to seed size and elongation pattern of internodes .
[ Sen. 9, subscore: 2.00 ]: As brassinosteroid signaling was known to be partially impaired in d1s , the sensitivity to 24-epibrassinolide ( 24-epiBL ) was compared among d1 alleles in a T65 genetic background .
[ Sen. 2, subscore: 1.00 ]: These mutants are called d1 .
[ Sen. 3, subscore: 1.00 ]: We have studied the expression profiles of the transcripts and translation products of rice Galpha in ten alleles of d1 including five additional alleles newly identified .
[ Sen. 4, subscore: 1.00 ]: By RT-PCR , the transcripts of the Galpha gene were detected in the all d1 alleles .
[ Sen. 6, subscore: 1.00 ]: In d1-4 , one amino acid change in the GTP-binding box A of the Galpha protein was occurred and even in this case the Galpha protein was only just detectable in the plasma membrane fraction .
[ Sen. 7, subscore: 1.00 ]: Given that the Galpha protein did not accumulate in the plasma membrane fraction in d1-8 which has a deletion of just a single amino acid in the Galpha protein , it is likely that a proper conformation of the Galpha is necessary for accumulation of Galpha protein in the plasma membrane .
[ Sen. 10, subscore: 1.00 ]: Only d1-4 showed responses similar to wild type rice .
[ Sen. 11, subscore: 1.00 ]: The results show that the d1-4 mutant is a mild allele in terms of the phenotype and mild hyposensitivity to the exogenously applied 24-epiBL .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 |
|---|
| Title: Rice dwarf mutant d1 , which is defective in the alpha subunit of the heterotrimeric G protein , affects gibberellin signal transduction .
| | Author: Ueguchi-Tanaka M Fujisawa Y Kobayashi M Ashikari M Iwasaki Y Kitano H Matsuoka M | | Journal: Proc . Natl . Acad . Sci . USA Citation: V : 97 ( 21 ) P : 11638-43 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11027362 Accession (PMID): 11027362 | | Abstract: Previously , we reported that the rice dwarf mutant , d1 , is defective in the alpha subunit of the heterotrimeric G protein ( Galpha ) .
In the present study , gibberellin ( GA ) signaling in d1 and the role of the Galpha protein in the GA-signaling pathway were investigated .
Compared with the wild type , GA induction of alpha-amylase activity in aleurone cells of d1 was greatly reduced .
Relative to the wild type , the GA ( 3 ) -treated aleurone layer of d1 had lower expression of Ramy1A , which encodes alpha-amylase , and OsGAMYB , which encodes a GA-inducible transcriptional factor , and no increase in expression of Ca ( 2 + ) -ATPase .
However , in the presence of high GA concentrations , alpha-amylase induction occurred even in d1 .
The GA sensitivity of second leaf sheath elongation in d1 was similar to that of the wild type in terms of dose responsiveness , but the response of internode elongation to GA was much lower in d1 .
Furthermore , Os20ox expression was up-regulated , and the GA content was elevated in the stunted internodes of d1 .
All these results suggest that d1 affects a part of the GA-signaling pathway , namely the induction of alpha-amylase in the aleurone layer and internode elongation .
In addition , a double mutant between d1 and another GA-signaling mutant , slr , revealed that SLR is epistatic to the D1 , supporting that the Galpha protein is involved in GA signaling .
However , the data also provide evidence for the presence of an alternative GA-signaling pathway that does not involve the Galpha protein .
It is proposed that GA signaling via the Galpha protein may be more sensitive than that of the alternative pathway , as indicated by the low GA responsiveness of this Galpha-independent pathway . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: The GA sensitivity of second leaf sheath elongation in d1 was similar to that of the wild type in terms of dose responsiveness , but the response of internode elongation to GA was much lower in d1 .
[ Sen. 9, subscore: 2.00 ]: In addition , a double mutant between d1 and another GA-signaling mutant , slr , revealed that SLR is epistatic to the D1 , supporting that the Galpha protein is involved in GA signaling .
[ Sen. 1, subscore: 1.00 ]: Previously , we reported that the rice dwarf mutant , d1 , is defective in the alpha subunit of the heterotrimeric G protein ( Galpha ) .
[ Sen. 2, subscore: 1.00 ]: In the present study , gibberellin ( GA ) signaling in d1 and the role of the Galpha protein in the GA-signaling pathway were investigated .
[ Sen. 3, subscore: 1.00 ]: Compared with the wild type , GA induction of alpha-amylase activity in aleurone cells of d1 was greatly reduced .
[ Sen. 4, subscore: 1.00 ]: Relative to the wild type , the GA ( 3 ) -treated aleurone layer of d1 had lower expression of Ramy1A , which encodes alpha-amylase , and OsGAMYB , which encodes a GA-inducible transcriptional factor , and no increase in expression of Ca ( 2 + ) -ATPase .
[ Sen. 5, subscore: 1.00 ]: However , in the presence of high GA concentrations , alpha-amylase induction occurred even in d1 .
[ Sen. 7, subscore: 1.00 ]: Furthermore , Os20ox expression was up-regulated , and the GA content was elevated in the stunted internodes of d1 .
[ Sen. 8, subscore: 1.00 ]: All these results suggest that d1 affects a part of the GA-signaling pathway , namely the induction of alpha-amylase in the aleurone layer and internode elongation .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 |
|---|
| Title: The interactions among DWARF10 , auxin and cytokinin underlie lateral bud outgrowth in rice .
| | Author: Zhang S Li G Fang J Chen W Jiang H Zou J Liu X Zhao X Li X Chu C Xie Q Jiang X Zhu L | | Journal: J Integr Plant Biol Citation: V : 52 P : 626-38 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20590993 Accession (PMID): 20590993 | | Abstract: Previous studies have shown that DWARF10 ( D10 ) is a rice ortholog of MAX4/RMS1/DAD1 , encoding a carotenoid cleavage dioxygenase and functioning in strigolactones/strigolactone-derivatives ( SL ) biosynthesis .
Here we use D10 RNA interference ( RNAi ) transgenic plants similar to d10 mutant in phenotypes to investigate the interactions among D10 , auxin and cytokinin in regulating rice shoot branching .
Auxin levels in node 1 of both decapitated D10-RNAi and wild type plants decreased significantly , showing that decapitation does reduce endogenous auxin concentration , but decapitation has no clear effects on auxin levels in node 2 of the same plants .
This implies that node 1 may be the location where a possible interaction between auxin and D10 gene would be detected .
D10 expression in node 1 is inhibited by decapitation , and this inhibition can be restored by exogenous auxin application , indicating that D10 may play an important role in auxin regulation of SL .
The decreased expression of most OsPINs in shoot nodes of D10-RNAi plants may cause a reduced auxin transport capacity .
Furthermore , effects of auxin treatment of decapitated plants on the expression of cytokinin biosynthetic genes suggest that D10 promotes cytokinin biosynthesis by reducing auxin levels .
Besides , in D10-RNAi plants , decreased storage cytokinin levels in the shoot node may partly account for the increased active cytokinin contents , resulting in more tillering phenotypes .
| Matching Sentences:
[ Sen. 2, subscore: 3.00 ]: Here we use D10 RNA interference ( RNAi ) transgenic plants similar to d10 mutant in phenotypes to investigate the interactions among D10 , auxin and cytokinin in regulating rice shoot branching .
[ Sen. 5, subscore: 2.00 ]: D10 expression in node 1 is inhibited by decapitation , and this inhibition can be restored by exogenous auxin application , indicating that D10 may play an important role in auxin regulation of SL .
[ Sen. 1, subscore: 1.00 ]: Previous studies have shown that DWARF10 ( D10 ) is a rice ortholog of MAX4/RMS1/DAD1 , encoding a carotenoid cleavage dioxygenase and functioning in strigolactones/strigolactone-derivatives ( SL ) biosynthesis .
[ Sen. 3, subscore: 1.00 ]: Auxin levels in node 1 of both decapitated D10-RNAi and wild type plants decreased significantly , showing that decapitation does reduce endogenous auxin concentration , but decapitation has no clear effects on auxin levels in node 2 of the same plants .
[ Sen. 4, subscore: 1.00 ]: This implies that node 1 may be the location where a possible interaction between auxin and D10 gene would be detected .
[ Sen. 6, subscore: 1.00 ]: The decreased expression of most OsPINs in shoot nodes of D10-RNAi plants may cause a reduced auxin transport capacity .
[ Sen. 7, subscore: 1.00 ]: Furthermore , effects of auxin treatment of decapitated plants on the expression of cytokinin biosynthetic genes suggest that D10 promotes cytokinin biosynthesis by reducing auxin levels .
[ Sen. 8, subscore: 1.00 ]: Besides , in D10-RNAi plants , decreased storage cytokinin levels in the shoot node may partly account for the increased active cytokinin contents , resulting in more tillering phenotypes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 9.00 |
|---|
| Title: DWARF10 , an RMS1/MAX4/DAD1 ortholog , controls lateral bud outgrowth in rice .
| | Author: Arite T Iwata H Ohshima K Maekawa M Nakajima M Kojima M Sakakibara H Kyozuka J | | Journal: Plant J Citation: V : 51 P : 1019-29 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17655651 Accession (PMID): 17655651 | | Abstract: Plant architecture is mostly determined by shoot branching patterns .
Apical dominance is a well-known control mechanism in the development of branching patterns , but little is known regarding its role in monocots such as rice .
Here , we show that the concept of apical dominance can be applied to tiller bud outgrowth of rice .
In dwarf10 ( d10 ) , an enhanced branching mutant of rice , apical dominance can be observed , but the inhibitory effects of the apical meristem was reduced .
D10 is a rice ortholog of MAX4/RMS1/DAD1 that encodes a carotenoid cleavage dioxygenase 8 and is supposed to be involved in the synthesis of an unidentified inhibitor of shoot branching .
D10 expression predominantly occurs in vascular cells in most organs .
Real-time polymerase chain reaction analysis revealed that accumulation of D10 mRNA is induced by exogenous auxin .
Moreover , D10 expression is upregulated in six branching mutants , d3 , d10 , d14 , d17 , d27 and high tillering dwarf ( htd1 ) .
No such effects were found for D3 or HTD1 , the MAX2 and MAX3 orthologs , respectively , of rice .
These findings imply that D10 transcription might be a critical step in the regulation of the branching inhibitor pathway .
In addition , we present observations that suggest that FINE CULM1 ( FC1 ) , a rice ortholog of teosinte branched 1 ( tb1 ) , possibly works independently of the branching inhibitor pathway .
| Matching Sentences:
[ Sen. 8, subscore: 4.00 ]: Moreover , D10 expression is upregulated in six branching mutants , d3 , d10 , d14 , d17 , d27 and high tillering dwarf ( htd1 ) .
[ Sen. 4, subscore: 1.00 ]: In dwarf10 ( d10 ) , an enhanced branching mutant of rice , apical dominance can be observed , but the inhibitory effects of the apical meristem was reduced .
[ Sen. 5, subscore: 1.00 ]: D10 is a rice ortholog of MAX4/RMS1/DAD1 that encodes a carotenoid cleavage dioxygenase 8 and is supposed to be involved in the synthesis of an unidentified inhibitor of shoot branching .
[ Sen. 6, subscore: 1.00 ]: D10 expression predominantly occurs in vascular cells in most organs .
[ Sen. 7, subscore: 1.00 ]: Real-time polymerase chain reaction analysis revealed that accumulation of D10 mRNA is induced by exogenous auxin .
[ Sen. 10, subscore: 1.00 ]: These findings imply that D10 transcription might be a critical step in the regulation of the branching inhibitor pathway .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
|---|
| Title: A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant , dwarf11 , with reduced seed length .
| | Author: Tanabe S Ashikari M Fujioka S Takatsuto S Yoshida S Yano M Yoshimura A Kitano H Matsuoka M Fujisawa Y Kato H Iwasaki Y | | Journal: Plant Cell Citation: V : 17 ( 3 ) P : 776-90 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15705958 Accession (PMID): 15705958 | | Abstract: We have characterized a rice ( Oryza sativa ) dwarf mutant , dwarf11 ( d11 ) , that bears seeds of reduced length .
To understand the mechanism by which seed length is regulated , the D11 gene was isolated by a map-based cloning method .
The gene was found to encode a novel cytochrome P450 ( CYP724B1 ) , which showed homology to enzymes involved in brassinosteroid ( BR ) biosynthesis .
The dwarf phenotype of d11 mutants was restored by the application of the brassinolide ( BL ) .
Compared with wild-type plants , the aberrant D11 mRNA accumulated at higher levels in d11 mutants and was dramatically reduced by treatment with BL , implying that the gene is feedback-regulated by BL .
Precise determination of the defective step ( s ) in BR synthesis in d11 mutants proved intractable because of it issue specificity and the complex control of BR accumulation in plants .
However , 6-deoxotyphasterol ( 6-DeoxoTY ) and typhasterol ( TY ) , but not any upstream intermediates before these compounds , effectively restored BR response in d11 mutants in a lamina joint bending assay .
Multiple lines of evidence together suggest that the D11/CYP724B1 gene plays a role in BR synthesis and may be involved in the supply of 6-DeoxoTY and TY in the BR biosynthesis network in rice . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Compared with wild-type plants , the aberrant D11 mRNA accumulated at higher levels in d11 mutants and was dramatically reduced by treatment with BL , implying that the gene is feedback-regulated by BL .
[ Sen. 1, subscore: 1.00 ]: We have characterized a rice ( Oryza sativa ) dwarf mutant , dwarf11 ( d11 ) , that bears seeds of reduced length .
[ Sen. 2, subscore: 1.00 ]: To understand the mechanism by which seed length is regulated , the D11 gene was isolated by a map-based cloning method .
[ Sen. 4, subscore: 1.00 ]: The dwarf phenotype of d11 mutants was restored by the application of the brassinolide ( BL ) .
[ Sen. 6, subscore: 1.00 ]: Precise determination of the defective step ( s ) in BR synthesis in d11 mutants proved intractable because of it issue specificity and the complex control of BR accumulation in plants .
[ Sen. 7, subscore: 1.00 ]: However , 6-deoxotyphasterol ( 6-DeoxoTY ) and typhasterol ( TY ) , but not any upstream intermediates before these compounds , effectively restored BR response in d11 mutants in a lamina joint bending assay .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
|---|
| Title: Function and expression pattern of the alpha subunit of the heterotrimeric G protein in rice .
| | Author: Izawa Y Takayanagi Y Inaba N Abe Y Minami M Fujisawa Y Kato H Ohki S Kitano H Iwasaki Y | | Journal: Plant Cell Physiol Citation: V : 51 P : 271-81 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20040584 Accession (PMID): 20040584 | | Abstract: The d1 mutant , which is deficient for the heterotrimeric G-protein alpha subunit ( Galpha ) gene of rice , shows dwarfism and sets small round seeds .
To determine whether dwarfism in d1 is due to a reduction in cell number or to shortened cell length , the cell number of the leaf sheath , the internode , the root and the lemma was compared between Nipponbare , a wild-type rice and d1-5 , a d1 allele derived from Nipponbare .
Our results indicate that the cell number was reduced in all organs analyzed in d1-5 .
In addition , cell enlargement was found in roots and lemma of d1-5 , although the organ length in d1-5 was shorter than that of wild-type rice .
These results suggest that rice Galpha participates in cell proliferation in rice .
Western blot analyses using anti-Galpha antibody and RT-PCR analyses indicate that Galpha is mostly expressed in the developing organs .
Galpha promoter activity studies using the GUS reporter gene confirmed that the expression of Galpha was highest in developing organs .
We conclude that rice Galpha participates in the regulation of cell number in a developmental stage-dependent manner .
| Matching Sentences:
[ Sen. 2, subscore: 3.00 ]: To determine whether dwarfism in d1 is due to a reduction in cell number or to shortened cell length , the cell number of the leaf sheath , the internode , the root and the lemma was compared between Nipponbare , a wild-type rice and d1-5 , a d1 allele derived from Nipponbare .
[ Sen. 4, subscore: 2.00 ]: In addition , cell enlargement was found in roots and lemma of d1-5 , although the organ length in d1-5 was shorter than that of wild-type rice .
[ Sen. 1, subscore: 1.00 ]: The d1 mutant , which is deficient for the heterotrimeric G-protein alpha subunit ( Galpha ) gene of rice , shows dwarfism and sets small round seeds .
[ Sen. 3, subscore: 1.00 ]: Our results indicate that the cell number was reduced in all organs analyzed in d1-5 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
|---|
| Title: Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the alpha-subunit of GTP-binding protein .
| | Author: Ashikari M Wu J Yano M Sasaki T Yoshimura A | | Journal: Proc . Natl . Acad . Sci . USA Citation: V : 96 ( 18 ) P : 10284-9 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10468600 Accession (PMID): 10468600 | | Abstract: A rice Dwarf 1 gene was identified by using a map-based cloning strategy .
Its recessive mutant allele confers a dwarf phenotype .
Linkage analysis revealed that a cDNA encoding the alpha-subunit of GTP-binding protein cosegregated with d1 in 3 , 185 d1 segregants .
Southern hybridization analysis with this cDNA as a probe showed different band patterns in several d1 mutant lines .
In at least four independent d1 mutants , no gene transcript was observed by Northern hybridization analysis .
Sequencing analysis revealed that an 833-bp deletion had occurred in one of the mutant alleles , which resulted in an inability to express GTP-binding protein .
A transgenic d1 mutant with GTP-binding protein gene restored the normal phenotype .
We conclude that the rice Dwarf 1 gene encodes GTP-binding protein and that the protein plays an important role in plant growth and development .
Because the d1 mutant is classified as gibberellin-insensitive , we suggest that the GTP-binding protein might be associated with gibberellin signal transduction .
| Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: Linkage analysis revealed that a cDNA encoding the alpha-subunit of GTP-binding protein cosegregated with d1 in 3 , 185 d1 segregants .
[ Sen. 4, subscore: 1.00 ]: Southern hybridization analysis with this cDNA as a probe showed different band patterns in several d1 mutant lines .
[ Sen. 5, subscore: 1.00 ]: In at least four independent d1 mutants , no gene transcript was observed by Northern hybridization analysis .
[ Sen. 7, subscore: 1.00 ]: A transgenic d1 mutant with GTP-binding protein gene restored the normal phenotype .
[ Sen. 9, subscore: 1.00 ]: Because the d1 mutant is classified as gibberellin-insensitive , we suggest that the GTP-binding protein might be associated with gibberellin signal transduction .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
|---|
| Title: Proteomic analysis of rice embryo : an approach for investigating Galpha protein-regulated proteins .
| | Author: Komatsu S Abbasi F Kobori E Fujisawa Y Kato H Iwasaki Y | | Journal: Proteomics Citation: V : 5 ( 15 ) P : 3932-41 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16152656 Accession (PMID): 16152656 | | Abstract: The rice dwarf1 ( d1 ) mutant , which lacks the alpha subunit of a heterotrimeric G protein ( Galpha protein ) , shows abnormal morphology due to shortened internodes , dark green leaves and grains that are small and round .
Proteome analysis was used in this study to aid in determining the function of Galpha protein in rice embryos .
Using 2-DE , seven seed embryo proteins were shown to be down-regulated in the d1 mutant as compared with its wild type .
These seven proteins included a receptor for activated C-kinase ( RACK ) and six rice embryo globulin-2 proteins ( REG2 ) .
The six REG2 have similar molecular masses with minor differences in pI .
In addition to the reduced accumulation of RACK in the d1 mutant , the increase in QL/d1 , in which a constitutively active form of the Galpha protein is expressed , was significantly higher as compared with wild type .
The level of accumulation of these seven proteins during seed development and maturation did not change significantly until the 2nd wk after pollination .
Reduced accumulation of these seven proteins started in the d1 mutant at the 3rd wk after pollination , and continued until seed maturation was complete .
All seven proteins were completely absent 24 h after imbibition in both d1 mutant and its wild type .
However , the phytohormone abscisic acid promoted the expression level of RACK after imbibition in the wild type as compared with d1 mutant .
These results suggest that RACK is regulated by Galpha-protein and plays an important role in a basic cellular process as well as in rice embryogenesis and germination . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice dwarf1 ( d1 ) mutant , which lacks the alpha subunit of a heterotrimeric G protein ( Galpha protein ) , shows abnormal morphology due to shortened internodes , dark green leaves and grains that are small and round .
[ Sen. 3, subscore: 1.00 ]: Using 2-DE , seven seed embryo proteins were shown to be down-regulated in the d1 mutant as compared with its wild type .
[ Sen. 6, subscore: 1.00 ]: In addition to the reduced accumulation of RACK in the d1 mutant , the increase in QL/d1 , in which a constitutively active form of the Galpha protein is expressed , was significantly higher as compared with wild type .
[ Sen. 8, subscore: 1.00 ]: Reduced accumulation of these seven proteins started in the d1 mutant at the 3rd wk after pollination , and continued until seed maturation was complete .
[ Sen. 9, subscore: 1.00 ]: All seven proteins were completely absent 24 h after imbibition in both d1 mutant and its wild type .
[ Sen. 10, subscore: 1.00 ]: However , the phytohormone abscisic acid promoted the expression level of RACK after imbibition in the wild type as compared with d1 mutant .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
|---|
| Title: Global patterns of gene expression in the aleurone of wild-type and dwarf1 mutant rice .
| | Author: Bethke PC Hwang YS Zhu T Jones RL .
| | Journal: Plant Physiol .
Citation: V : 140 ( 2 ) P : 484-98 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16384900 Accession (PMID): 16384900 | | Abstract: The cereal aleurone layer is a model system for studying the regulation of transcription by gibberellin ( GA ) and abscisic acid ( ABA ) .
GA stimulates and ABA prevents the transcription of genes for alpha-amylases and other secreted hydrolytic enzymes , but how GA and ABA affect the transcription of other genes is largely unknown .
We characterized gene expression in rice ( Oryza sativa ) aleurone using a half-genome rice microarray .
Of the 23 , 000 probe sets on the chip , approximately 11 , 000 hybridized with RNA from rice aleurone treated with ABA , GA , or no hormone .
As expected , GA regulated the expression of many genes , and 3 times as many genes were up-regulated by GA at 8 h than were down-regulated .
Changes in gene expression resulting from ABA treatment were not consistent with the hypothesis that the role of ABA in this it issue is primarily to repress gene expression , and 10 times more genes were up-regulated by ABA at 8 h than were down-regulated by ABA .
We also measured transcript abundance in aleurone of dwarf1 ( d1 ) mutant rice .
The d1 protein is the sole alpha-subunit of heterotrimeric G-proteins in rice .
Genes up-regulated by GA or ABA had higher expression in wild type than in d1 aleurone , and genes down-regulated by GA had lower expression in wild type relative to d1 aleurone .
The d1 mutation did not result in a decrease in sensitivity to GA at the level of transcription .
Rather , changes in transcript abundance were smaller in the d1 mutant than in wild type . | Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: Genes up-regulated by GA or ABA had higher expression in wild type than in d1 aleurone , and genes down-regulated by GA had lower expression in wild type relative to d1 aleurone .
[ Sen. 7, subscore: 1.00 ]: We also measured transcript abundance in aleurone of dwarf1 ( d1 ) mutant rice .
[ Sen. 8, subscore: 1.00 ]: The d1 protein is the sole alpha-subunit of heterotrimeric G-proteins in rice .
[ Sen. 10, subscore: 1.00 ]: The d1 mutation did not result in a decrease in sensitivity to GA at the level of transcription .
[ Sen. 11, subscore: 1.00 ]: Rather , changes in transcript abundance were smaller in the d1 mutant than in wild type .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
