| 6 matches found in 4 documents. Search time: 0.002 seconds. |
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Score: 3.00 |
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| Title: Rice APOPTOSIS INHIBITOR5 coupled with two DEAD-box adenosine 5-triphosphate-dependent RNA helicases regulates tapetum degeneration .
| | Author: Li X Gao X Wei Y Deng L Ouyang Y Chen G Li X Zhang Q Wu C | | Journal: Plant Cell Citation: V : 23 P : 1416-34 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21467577 Accession (PMID): 21467577 | | Abstract: Programmed cell death ( PCD ) during tapetum degeneration in postmeiotic anthers is critical for the proper development of male gametophytes in flowering plants .
Although several genes involved in this process have been identified recently , the molecular mechanism is still poorly understood .
Here , we show that knockout of rice ( Oryza sativa ) APOPTOSIS INHIBITOR5 ( API5 ) , which encodes a putative homolog of antiapoptosis protein Api5 in animals , results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes .
Os API5 is a nuclear protein that interacts with two DEAD-box ATP-dependent RNA helicases , API5-INTERACTING PROTEIN1 ( AIP1 ) and AIP2 .
AIP1 and AIP2 are homologs of yeast ( Saccharomyces cerevisiae ) Suppressor of Bad Response to Refrigeration1 protein 2 ( SUB2p ) that have critical roles in transcription elongation and pre-mRNA splicing .
Os AIP1 and AIP2 can form dimers and interact directly with the promoter region of CP1 , a rice cysteine protease gene .
Suppression of Os AIP1/2 leads to down-regulation of CP1 , resulting in sterility , which is highly similar to the effects of suppressed expression of Os CP1 .
Our results uncover a previously unknown pathway for regulating PCD during tapetum degeneration in rice , one that may be conserved among eukaryotic organisms .
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[ Sen. 7, subscore: 2.00 ]: Programmed cell death ( PCD ) during tapetum degeneration in postmeiotic anthers is critical for the proper development of male gametophytes in flowering plants . Although several genes involved in this process have been identified recently , the molecular mechanism is still poorly understood . Here , we show that knockout of rice ( Oryza sativa ) APOPTOSIS INHIBITOR5 ( API5 ) , which encodes a putative homolog of antiapoptosis protein Api5 in animals , results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes . Os API5 is a nuclear protein that interacts with two DEAD-box ATP-dependent RNA helicases , API5-INTERACTING PROTEIN1 ( AIP1 ) and AIP2 . AIP1 and AIP2 are homologs of yeast ( Saccharomyces cerevisiae ) Suppressor of Bad Response to Refrigeration1 protein 2 ( SUB2p ) that have critical roles in transcription elongation and pre-mRNA splicing . Os AIP1 and AIP2 can form dimers and interact directly with the promoter region of CP1 , a rice cysteine protease gene . Suppression of Os AIP1/2 leads to down-regulation of CP1 , resulting in sterility , which is highly similar to the effects of suppressed expression of Os CP1 . Our results uncover a previously unknown pathway for regulating PCD during tapetum degeneration in rice , one that may be conserved among eukaryotic organisms .
[ Sen. 6, subscore: 1.00 ]: Programmed cell death ( PCD ) during tapetum degeneration in postmeiotic anthers is critical for the proper development of male gametophytes in flowering plants . Although several genes involved in this process have been identified recently , the molecular mechanism is still poorly understood . Here , we show that knockout of rice ( Oryza sativa ) APOPTOSIS INHIBITOR5 ( API5 ) , which encodes a putative homolog of antiapoptosis protein Api5 in animals , results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes . Os API5 is a nuclear protein that interacts with two DEAD-box ATP-dependent RNA helicases , API5-INTERACTING PROTEIN1 ( AIP1 ) and AIP2 . AIP1 and AIP2 are homologs of yeast ( Saccharomyces cerevisiae ) Suppressor of Bad Response to Refrigeration1 protein 2 ( SUB2p ) that have critical roles in transcription elongation and pre-mRNA splicing . Os AIP1 and AIP2 can form dimers and interact directly with the promoter region of CP1 , a rice cysteine protease gene . Suppression of Os AIP1/2 leads to down-regulation of CP1 , resulting in sterility , which is highly similar to the effects of suppressed expression of Os CP1 . Our results uncover a previously unknown pathway for regulating PCD during tapetum degeneration in rice , one that may be conserved among eukaryotic organisms .
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Score: 2.00 |
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| Title: Immunological characterization of rice tungro spherical virus coat proteins and differentiation of isolates from the Philippines and India .
| | Author: Druka A Burns T Zhang S Hull R | | Journal: J Gen . Virol . Citation: V : 77 ( Pt 8 ) ( ) P : 1975-83 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8760450 Accession (PMID): 8760450 | | Abstract: Rice tungro spherical virus ( RTSV ) has an RNA genome of more than 12 kb with various features which classify it as a plant picornavirus .
The capsid comprises three coat protein ( CP ) species , CP1 , CP2 and CP3 , with predicted molecular masses of 22 . 5 , 22 . 0 and 33 kDa , respectively , which are cleaved from a polyprotein .
In order to obtain information on the properties of these proteins , each was expressed in E coli , purified as a fusion to the maltose-binding protein and used for raising a polyclonal antiserum .
CP1 , CP2 and CP3 with the expected molecular masses were detected specifically in virus preparations .
CP3 is probably the major antigenic determinant on the surface of RTSV particles , as was shown by ELISA , Western blotting and immunogold electron microscopy using antisera obtained against whole virus particles and to each CP separately .
In some cases , especially in crude extracts , CP3 antiserum detected several other proteins ( 40-42 kDa ) , which could be products of CP3 post-translational modification .
No serological differences were detected between the three CPs from isolates from the Philippines , Thailand , Malaysia and India .
The CP3-related 40-42 kDa proteins of the Indian RTSV isolate have a slightly higher electrophoretic mobility ( 42-44 kDa ) and a different response to cellulolytic enzyme preparations , which allows them to be differentiated from south-east Asian isolates . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Rice tungro spherical virus ( RTSV ) has an RNA genome of more than 12 kb with various features which classify it as a plant picornavirus . The capsid comprises three coat protein ( CP ) species , CP1 , CP2 and CP3 , with predicted molecular masses of 22 . 5 , 22 . 0 and 33 kDa , respectively , which are cleaved from a polyprotein . In order to obtain information on the properties of these proteins , each was expressed in E coli , purified as a fusion to the maltose-binding protein and used for raising a polyclonal antiserum . CP1 , CP2 and CP3 with the expected molecular masses were detected specifically in virus preparations . CP3 is probably the major antigenic determinant on the surface of RTSV particles , as was shown by ELISA , Western blotting and immunogold electron microscopy using antisera obtained against whole virus particles and to each CP separately . In some cases , especially in crude extracts , CP3 antiserum detected several other proteins ( 40-42 kDa ) , which could be products of CP3 post-translational modification . No serological differences were detected between the three CPs from isolates from the Philippines , Thailand , Malaysia and India . The CP3-related 40-42 kDa proteins of the Indian RTSV isolate have a slightly higher electrophoretic mobility ( 42-44 kDa ) and a different response to cellulolytic enzyme preparations , which allows them to be differentiated from south-east Asian isolates .
[ Sen. 4, subscore: 1.00 ]: Rice tungro spherical virus ( RTSV ) has an RNA genome of more than 12 kb with various features which classify it as a plant picornavirus . The capsid comprises three coat protein ( CP ) species , CP1 , CP2 and CP3 , with predicted molecular masses of 22 . 5 , 22 . 0 and 33 kDa , respectively , which are cleaved from a polyprotein . In order to obtain information on the properties of these proteins , each was expressed in E coli , purified as a fusion to the maltose-binding protein and used for raising a polyclonal antiserum . CP1 , CP2 and CP3 with the expected molecular masses were detected specifically in virus preparations . CP3 is probably the major antigenic determinant on the surface of RTSV particles , as was shown by ELISA , Western blotting and immunogold electron microscopy using antisera obtained against whole virus particles and to each CP separately . In some cases , especially in crude extracts , CP3 antiserum detected several other proteins ( 40-42 kDa ) , which could be products of CP3 post-translational modification . No serological differences were detected between the three CPs from isolates from the Philippines , Thailand , Malaysia and India . The CP3-related 40-42 kDa proteins of the Indian RTSV isolate have a slightly higher electrophoretic mobility ( 42-44 kDa ) and a different response to cellulolytic enzyme preparations , which allows them to be differentiated from south-east Asian isolates .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development .
| | Author: Li N Zhang DS Liu HS Yin CS Li XX Liang WQ Yuan Z Xu B Chu HW Wang J Wen TQ Huang H Luo D Ma H Zhang DB .
| | Journal: Plant Cell Citation: V : 18 ( 11 ) P : 2999-3014 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17138695 Accession (PMID): 17138695 | | Abstract: In flowering plants , tapetum degeneration is proposed to be triggered by a programmed cell death ( PCD ) process during late stages of pollen development ; the PCD is thought to provide cellular contents supporting pollen wall formation and to allow the subsequent pollen release .
However , the molecular basis regulating tapetum PCD in plants remains poorly understood .
We report the isolation and characterization of a rice ( Oryza sativa ) male sterile mutant tapetum degeneration retardation ( tdr ) , which exhibits degeneration retardation of the tapetum and middle layer as well as collapse of microspores .
The TDR gene is preferentially expressed in the tapetum and encodes a putative basic helix-loop-helix protein , which is likely localized to the nucleus .
More importantly , two genes , Os CP1 and Os c6 , encoding a Cys protease and a protease inhibitor , respectively , were shown to be the likely direct targets of TDR through chromatin immunoprecipitation analyses and the electrophoretic mobility shift assay .
These results indicate that TDR is a key component of the molecular network regulating rice tapetum development and degeneration . | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: In flowering plants , tapetum degeneration is proposed to be triggered by a programmed cell death ( PCD ) process during late stages of pollen development ; the PCD is thought to provide cellular contents supporting pollen wall formation and to allow the subsequent pollen release . However , the molecular basis regulating tapetum PCD in plants remains poorly understood . We report the isolation and characterization of a rice ( Oryza sativa ) male sterile mutant tapetum degeneration retardation ( tdr ) , which exhibits degeneration retardation of the tapetum and middle layer as well as collapse of microspores . The TDR gene is preferentially expressed in the tapetum and encodes a putative basic helix-loop-helix protein , which is likely localized to the nucleus . More importantly , two genes , Os CP1 and Os c6 , encoding a Cys protease and a protease inhibitor , respectively , were shown to be the likely direct targets of TDR through chromatin immunoprecipitation analyses and the electrophoretic mobility shift assay . These results indicate that TDR is a key component of the molecular network regulating rice tapetum development and degeneration .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Sequences of the three coat protein genes of a Malaysian isolate of rice tungro spherical virus reveal a close relationship to the Philippine isolate .
| | Author: Zhang S Davies JW Hull R | | Journal: Virus Genes Citation: V : 15 ( 1 ) P : 61-4 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9354271 Accession (PMID): 9354271 | | Abstract: Coat protein genes CP1 , CP2 and CP3 of an isolate ( MaP1 ) of rice tungro spherical virus ( RTSV ) from Malaysia were isolated , cloned and sequenced .
Comparative analysis indicated that MaP1 isolate is closely related to the Philippine isolate . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Coat protein genes CP1 , CP2 and CP3 of an isolate ( MaP1 ) of rice tungro spherical virus ( RTSV ) from Malaysia were isolated , cloned and sequenced . Comparative analysis indicated that MaP1 isolate is closely related to the Philippine isolate .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
