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63 matches found in 28 documents. Search time: 0.003 seconds.
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Score: 10.00
Title: Characterization of beta-glucosidase from a strain of Penicillium purpurogenum KJS506 .
Author: Jeya M Joo AR Lee KM Tiwari MK Lee KM Kim SH Lee JK
Journal: Appl Microbiol Biotechnol Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20043150
Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: An extracellular BGL was purified to homogeneity by sequential chromatography of P purpurogenum culture supernatants , and the purified BGL showed higher activity ( V ( max ) = 934 U mg protein ( -1 ) ) than most BGLs from other sources .
[ Sen. 2, subscore: 2.00 ]: When rice straw and corn steep powder were used as carbon and nitrogen sources , respectively , the maximal BGL activity of 12 . 3 U ml ( -1 ) , one of the highest levels among BGL-producing microorganisms was observed .
[ Sen. 1, subscore: 1.00 ]: A novel beta-glucosidase ( BGL ) -producing strain was isolated and identified as Penicillium purpurogenum KJS506 based on its morphology and internal transcribed spacer ( ITS ) rDNA gene sequence .
[ Sen. 3, subscore: 1.00 ]: The optimum temperature and pH for BGL production were 32 degrees C and 4 , respectively .
[ Sen. 5, subscore: 1.00 ]: The complete ORF of bgl3 was cloned from P purpurogenum by a modified thermal asymmetric interlaced polymerase chain reaction .
[ Sen. 6, subscore: 1.00 ]: The bgl3 gene consists of a 2 , 571-bp ORF and encodes a putative protein containing 856 amino acids with a calculated molecular mass of 89 , 624 Da .
[ Sen. 8, subscore: 1.00 ]: The present results should contribute to improved industrial production of BGL by P purpurogenum KJS506 .
Score: 7.00
Title: Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition .
Author: Pengthaisong S Chen CF Withers SG Kuaprasert B Ketudat Cairns JR
Journal: Carbohydr Res Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub22418094
Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Inhibition of the BGlu1 beta-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1 .
[ Sen. 1, subscore: 1.00 ]: The rice BGlu1 beta-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from alpha-d-glucopyranosyl fluoride ( GlcF ) donor and p-nitrophenyl ( pNP ) cellobioside ( Glc2-pNP ) or cello-oligosaccharide acceptors .
[ Sen. 2, subscore: 1.00 ]: When activity with other donors and acceptors was tested , the initial enzyme preparation cleaved pNP-beta-D-glucopyranoside ( Glc-pNP ) and pNP-beta-D-fucopyranoside ( Fuc-pNP ) to pNP and glucose and fucose , suggesting contamination with wild type BGlu1 beta-glucosidase .
[ Sen. 3, subscore: 1.00 ]: The products from reaction of GlcF and Fuc-pNP included Fuc-beta- ( 1-->3 ) -Fuc-pNP , Glc-beta- ( 1-->3 ) -Fuc-pNP , and Fuc-beta- ( 1-->4 ) -Glc-beta- ( 1-->3 ) -Fuc-pNP , suggesting the presence of both wild type BGlu1 and its glycosynthase .
[ Sen. 5, subscore: 1.00 ]: Rice BGlu1 E386G-2 , generated from a new construct designed to minimize back-mutation , showed glycosynthase activity without wild type hydrolytic or transglycosylation activity .
[ Sen. 10, subscore: 1.00 ]: Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities .
Score: 6.00
Title: Complex arrangement of dispersed repeated DNA sequences in Oryza officinalis .
Author: Kiefer-Meyer MC Reddy AS Delseny M
Journal: Genome Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub8851805
Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: From one genomic clone , the sequences adjacent to the BglII element were subcloned and used as probes to demonstrate that the sequences flanking the BglII element are variable in different genomic clones and that some of them are also dispersed repetitive sequences .
[ Sen. 1, subscore: 1.00 ]: A 525-bp BglII fragment was isolated from Oryza officinalis DNA ( accession W1278 ) and shown to correspond to a new dispersed repetitive DNA sequence with specificity restricted to a subset of the wild rice with a C genome .
[ Sen. 4, subscore: 1.00 ]: Larger genomic clones ( 12-18 kbp ) were isolated and all contain sequences homologous to the BglII element .
[ Sen. 5, subscore: 1.00 ]: Analysis of these clones confirms that the BglII element is dispersed in the O officinalis genome .
[ Sen. 7, subscore: 1.00 ]: The genomic specificity of two of these dispersed repeats was evaluated and shown to be different from that of the initial BglII element .
Score: 5.00
Title: Expression of thermostable bacterial beta-glucosidase ( BglB ) in transgenic tobacco plants .
Author: Jung S Kim S Bae H Lim HS Bae HJ
Journal: Bioresour Technol Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20427180
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Transgenic tobacco plants expressing the hyperthermostable beta-glucosidase BglB of Thermotoga maritima were generated with the goal of cost-effective production of the enzyme for the application in bioconversion of lignocellulosic biomass .
[ Sen. 3, subscore: 1.00 ]: The optimal temperature and pH of the plant-expressed BglB was 80 degrees C and 4 . 5 , respectively .
[ Sen. 4, subscore: 1.00 ]: BglB activity was preserved in leaves after lyophilization , but decreased by over 70% with drying at room temperature .
[ Sen. 5, subscore: 1.00 ]: When BglB was synergistically supplied in a 1% ( w/v ) rice straw with Cel5A for efficient cellulase conversion , a 37% increase in glucose was observed .
[ Sen. 6, subscore: 1.00 ]: This report demonstrates the potential of utilizing transgenic tobacco for mass production of BglB .
Score: 4.00
Title: Purification , crystallization and preliminary X-ray analysis of rice BGlu1 beta-glucosidase with and without 2-deoxy-2-fluoro-beta-D-glucoside .
Author: Chuenchor W Pengthaisong S Yuvaniyama J Opassiri R Svasti J Ketudat Cairns JR .
Journal: Acta Crystallograph . Sect . F Struct . Biol . Cryst Commun . Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub16880561
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice ( Oryza sativa ) BGlu1 beta-glucosidase was expressed in Escherichia coli with N-terminal thioredoxin and hexahistidine tags and purified by immobilized metal-affinity chromatography ( IMAC ) .
[ Sen. 2, subscore: 1.00 ]: After removal of the N-terminal tags , cation-exchange and S-200 gel-filtration chromatography yielded a 50 kDa BGlu1 with >95% purity .
[ Sen. 4, subscore: 1.00 ]: Small tetragonal crystals of BGlu1 with and without inhibitor grew in 18% ( w/v ) PEG 8000 with 0 . 1 M sodium cacodylate pH 6 . 5 and 0 . 2 M zinc acetate .
[ Sen. 5, subscore: 1.00 ]: Crystals of BGlu1 with inhibitor were streak-seeded into 23% ( w/v ) PEG MME 5000 , 0 . 2 M ammonium sulfate , 0 . 1 M MES pH 6 . 7 to yield larger crystals .
Score: 4.00
Title: Enzymatic synthesis of cello-oligosaccharides by rice BGlu1 { beta } -glucosidase glycosynthase mutants .
Author: Hommalai G Withers SG Chuenchor W Cairns JR Svasti J
Journal: Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub17405771
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice ( BGlu1 ) beta-glucosidase is a glycosyl hydrolase family 1 enzyme that acts as an exoglucanase on beta- ( 1 , 4 ) - and short beta- ( 1 , 3 ) -linked gluco-oligosaccharides .
[ Sen. 2, subscore: 1.00 ]: Mutations of BGlu1 beta-glucosidase at glutamate residue 414 of its natural precursor destroyed the enzymes catalytic activity , but the enzyme could be rescued in the presence of the anionic nucleophiles formate and azide , which verifies that this residue is the catalytic nucleophile .
[ Sen. 8, subscore: 1.00 ]: The BGlu1 E414G glycosynthase was found to prefer longer chain length oligosaccharides that occupy at least three sugar residue binding subsites as acceptors for productive transglucosylation .
[ Sen. 9, subscore: 1.00 ]: This is the first report of a beta-glucansynthase derived from an exoglycosidase that can produce long chain cello-oligosaccharides , which likely reflects the extended oligosaccharide binding site of rice BGlu1 beta-glucosidase .
Score: 3.00
Title: Beta-glucosidase , exo-beta-glucanase and pyridoxine transglucosylase activities of rice BGlu1 .
Author: Opassiri R Hua Y Wara-Aswapati O Akiyama T Svasti J Esen A Ketudat Cairns JR .
Journal: Biochem . J Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub14692878
Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: The bglu1 cDNA for a beta-glucosidase cloned from rice ( Oryza sativa L ) seedlings was expressed as a soluble and active protein in Escherichia coli and designated BGlu1 .
[ Sen. 6, subscore: 1.00 ]: BGlu1 also had high transglucosylation activity towards pyridoxine , producing pyridoxine 5-O-beta-D-glucopyranoside in the presence of the glucose donor p-nitrophenyl beta-D-glucoside .
Score: 3.00
Title: Structural insights into rice BGlu1 beta-glucosidase oligosaccharide hydrolysis and transglycosylation .
Author: Chuenchor W Pengthaisong S Robinson RC Yuvaniyama J Oonanant W Bevan DR Esen A Chen CJ Opassiri R Svasti J Cairns JR
Journal: J Mol Biol Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub18308333
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The structures of rice BGlu1 beta-glucosidase , a plant beta-glucosidase active in hydrolyzing cell wall-derived oligosaccharides , and its covalent intermediate with 2-deoxy-2-fluoroglucoside have been solved at 2 . 2 A and 1 . 55 A resolution , respectively .
[ Sen. 5, subscore: 1.00 ]: The coordination of the hydroxyl groups suggests that sugars are positioned as acceptors for transglycosylation by their interactions with E176 , the catalytic acid/base , and Y131 , which is conserved in barley BGQ60/beta-II beta-glucosidase , that has oligosaccharide hydrolysis and transglycosylation activity similar to rice BGlu1 .
[ Sen. 6, subscore: 1.00 ]: As the rice and barley enzymes have different preferences for cellobiose and cellotriose , residues that appeared to interact with docked oligosaccharides were mutated to those of the barley enzyme to see if the relative activities of rice BGlu1 toward these substrates could be changed to those of BGQ60 .
Score: 3.00
Title: The structural basis of oligosaccharide binding by rice BGlu1 beta-glucosidase .
Author: Chuenchor W Pengthaisong S Robinson RC Yuvaniyama J Svasti J Cairns JR
Journal: J Struct Biol Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20884352
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice BGlu1 beta-glucosidase is an oligosaccharide exoglucosidase that binds to six beta- ( 1-->4 ) -linked glucosyl residues in its active site cleft .
[ Sen. 2, subscore: 1.00 ]: Here , we demonstrate that a BGlu1 E176Q active site mutant can be effectively rescued by small nucleophiles , such as acetate , azide and ascorbate , for hydrolysis of aryl glycosides in a pH-independent manner above pH5 , consistent with the role of E176 as the catalytic acid-base .
[ Sen. 4, subscore: 1.00 ]: The structures of the BGlu1 E176Q , its complexes with cellotetraose , cellopentaose and laminaribiose , and its covalent intermediate with 2-deoxy-2-fluoroglucoside were determined at 1 . 65 , 1 . 95 , 1 . 80 , 2 . 80 , and 1 . 90A resolution , respectively .
Score: 3.00
Title: The rice bright green leaf ( bgl ) locus encodes OsRopGEF10 , which activates the development of small cuticular papillae on leaf surfaces .
Author: Yoo JH Park JH Cho SH Yoo SC Li J Zhang H Kim KS Koh HJ Paek NC
Journal: Plant Mol Biol Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub22038138
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: In the paddy field , the bright green leaf ( bgl ) mutants of rice ( Oryza sativa ) exhibit a luminous green color that is clearly distinguishable from the normal green of wild-type plants .
[ Sen. 3, subscore: 1.00 ]: Transmission and scanning electron microscopy revealed that small cuticular papillae ( or small papillae ; SP ) , nipple-like structures , are absent on the adaxial and abaxial leaf surfaces of bgl mutants , leading to more direct reflection and less diffusion of green light .
[ Sen. 4, subscore: 1.00 ]: Map-based cloning revealed that the bgl locus encodes OsRopGEF10 , one of eleven OsRopGEFs in rice .

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