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63 matches found in 28 documents. Search time: 0.002 seconds.
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Score: 10.00
Title: Characterization of beta-glucosidase from a strain of Penicillium purpurogenum KJS506 .
Author: Jeya M Joo AR Lee KM Tiwari MK Lee KM Kim SH Lee JK
Literature: oryza Field: abstract Doc ID: pub20043150
Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: An extracellular BGL was purified to homogeneity by sequential chromatography of P purpurogenum culture supernatants , and the purified BGL showed higher activity ( V ( max ) = 934 U mg protein ( -1 ) ) than most BGLs from other sources .
[ Sen. 2, subscore: 2.00 ]: When rice straw and corn steep powder were used as carbon and nitrogen sources , respectively , the maximal BGL activity of 12 . 3 U ml ( -1 ) , one of the highest levels among BGL-producing microorganisms was observed .
[ Sen. 1, subscore: 1.00 ]: A novel beta-glucosidase ( BGL ) -producing strain was isolated and identified as Penicillium purpurogenum KJS506 based on its morphology and internal transcribed spacer ( ITS ) rDNA gene sequence .
[ Sen. 3, subscore: 1.00 ]: The optimum temperature and pH for BGL production were 32 degrees C and 4 , respectively .
[ Sen. 5, subscore: 1.00 ]: The complete ORF of bgl3 was cloned from P purpurogenum by a modified thermal asymmetric interlaced polymerase chain reaction .
[ Sen. 6, subscore: 1.00 ]: The bgl3 gene consists of a 2 , 571-bp ORF and encodes a putative protein containing 856 amino acids with a calculated molecular mass of 89 , 624 Da .
[ Sen. 8, subscore: 1.00 ]: The present results should contribute to improved industrial production of BGL by P purpurogenum KJS506 .
Score: 7.00
Title: Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition .
Author: Pengthaisong S Chen CF Withers SG Kuaprasert B Ketudat Cairns JR
Literature: oryza Field: abstract Doc ID: pub22418094
Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Inhibition of the BGlu1 beta-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1 .
[ Sen. 1, subscore: 1.00 ]: The rice BGlu1 beta-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from alpha-d-glucopyranosyl fluoride ( GlcF ) donor and p-nitrophenyl ( pNP ) cellobioside ( Glc2-pNP ) or cello-oligosaccharide acceptors .
[ Sen. 2, subscore: 1.00 ]: When activity with other donors and acceptors was tested , the initial enzyme preparation cleaved pNP-beta-D-glucopyranoside ( Glc-pNP ) and pNP-beta-D-fucopyranoside ( Fuc-pNP ) to pNP and glucose and fucose , suggesting contamination with wild type BGlu1 beta-glucosidase .
[ Sen. 3, subscore: 1.00 ]: The products from reaction of GlcF and Fuc-pNP included Fuc-beta- ( 1-->3 ) -Fuc-pNP , Glc-beta- ( 1-->3 ) -Fuc-pNP , and Fuc-beta- ( 1-->4 ) -Glc-beta- ( 1-->3 ) -Fuc-pNP , suggesting the presence of both wild type BGlu1 and its glycosynthase .
[ Sen. 5, subscore: 1.00 ]: Rice BGlu1 E386G-2 , generated from a new construct designed to minimize back-mutation , showed glycosynthase activity without wild type hydrolytic or transglycosylation activity .
[ Sen. 10, subscore: 1.00 ]: Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities .
Score: 6.00
Title: Complex arrangement of dispersed repeated DNA sequences in Oryza officinalis .
Author: Kiefer-Meyer MC Reddy AS Delseny M
Literature: oryza Field: abstract Doc ID: pub8851805
Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: From one genomic clone , the sequences adjacent to the BglII element were subcloned and used as probes to demonstrate that the sequences flanking the BglII element are variable in different genomic clones and that some of them are also dispersed repetitive sequences .
[ Sen. 1, subscore: 1.00 ]: A 525-bp BglII fragment was isolated from Oryza officinalis DNA ( accession W1278 ) and shown to correspond to a new dispersed repetitive DNA sequence with specificity restricted to a subset of the wild rice with a C genome .
[ Sen. 4, subscore: 1.00 ]: Larger genomic clones ( 12-18 kbp ) were isolated and all contain sequences homologous to the BglII element .
[ Sen. 5, subscore: 1.00 ]: Analysis of these clones confirms that the BglII element is dispersed in the O officinalis genome .
[ Sen. 7, subscore: 1.00 ]: The genomic specificity of two of these dispersed repeats was evaluated and shown to be different from that of the initial BglII element .
Score: 5.00
Title: Expression of thermostable bacterial beta-glucosidase ( BglB ) in transgenic tobacco plants .
Author: Jung S Kim S Bae H Lim HS Bae HJ
Literature: oryza Field: abstract Doc ID: pub20427180
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Transgenic tobacco plants expressing the hyperthermostable beta-glucosidase BglB of Thermotoga maritima were generated with the goal of cost-effective production of the enzyme for the application in bioconversion of lignocellulosic biomass .
[ Sen. 3, subscore: 1.00 ]: The optimal temperature and pH of the plant-expressed BglB was 80 degrees C and 4 . 5 , respectively .
[ Sen. 4, subscore: 1.00 ]: BglB activity was preserved in leaves after lyophilization , but decreased by over 70% with drying at room temperature .
[ Sen. 5, subscore: 1.00 ]: When BglB was synergistically supplied in a 1% ( w/v ) rice straw with Cel5A for efficient cellulase conversion , a 37% increase in glucose was observed .
[ Sen. 6, subscore: 1.00 ]: This report demonstrates the potential of utilizing transgenic tobacco for mass production of BglB .
Score: 4.00
Title: Purification , crystallization and preliminary X-ray analysis of rice BGlu1 beta-glucosidase with and without 2-deoxy-2-fluoro-beta-D-glucoside .
Author: Chuenchor W Pengthaisong S Yuvaniyama J Opassiri R Svasti J Ketudat Cairns JR .
Literature: oryza Field: abstract Doc ID: pub16880561
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice ( Oryza sativa ) BGlu1 beta-glucosidase was expressed in Escherichia coli with N-terminal thioredoxin and hexahistidine tags and purified by immobilized metal-affinity chromatography ( IMAC ) .
[ Sen. 2, subscore: 1.00 ]: After removal of the N-terminal tags , cation-exchange and S-200 gel-filtration chromatography yielded a 50 kDa BGlu1 with >95% purity .
[ Sen. 4, subscore: 1.00 ]: Small tetragonal crystals of BGlu1 with and without inhibitor grew in 18% ( w/v ) PEG 8000 with 0 . 1 M sodium cacodylate pH 6 . 5 and 0 . 2 M zinc acetate .
[ Sen. 5, subscore: 1.00 ]: Crystals of BGlu1 with inhibitor were streak-seeded into 23% ( w/v ) PEG MME 5000 , 0 . 2 M ammonium sulfate , 0 . 1 M MES pH 6 . 7 to yield larger crystals .

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