Score: 5.00 | Title: Evidence of an auxin signal pathway , microRNA167-ARF8-GH3 , and its response to exogenous auxin in cultured rice cells .
| Author: Yang JH Han SJ Yoon EK Lee WS .
| Journal: Nucleic Acids Res .
Citation: V : 34 ( 6 ) P : 1892-9 Year: Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub16598073 | Abstract: MicroRNA167 ( miR167 ) was shown to cleave auxin responsive factor 8 ( ARF8 ) mRNA in cultured rice cells .
MiR167 level was found to be controlled by the presence of auxin in the growth medium .
When cells grew in auxin-free medium , miR167 level decreased , resulting in an increase in the level of ARF8 mRNA .
Cells growing in the normal growth medium containing auxin showed a reversed trend .
It was also shown that expression of OsGH3-2 , an rice IAA-conjugating enzyme , was positively regulated by ARF8 .
Delivery of synthesized miR167 into cells led to decrease of both ARF8 mRNA and OsGH3-2 mRNA .
This study provides an evidence in which the exogeneous auxin signal is transduced to OsGH3-2 through miR167 and ARF8 in sequence .
This proposed auxin signal transduction pathway , auxin-miR167-ARF8-OsGH3-2 , could be , in conjunction with the other microRNA-mediated auxin signals , an important one for responding to exogeneous auxin and for determining the cellular free auxin level which guides appropriate auxin responses . | Matching Sentences: [ Sen. 1, subscore: 1.00 ]: MicroRNA167 ( miR167 ) was shown to cleave auxin responsive factor 8 ( ARF8 ) mRNA in cultured rice cells . [ Sen. 3, subscore: 1.00 ]: When cells grew in auxin-free medium , miR167 level decreased , resulting in an increase in the level of ARF8 mRNA . [ Sen. 5, subscore: 1.00 ]: It was also shown that expression of OsGH3-2 , an rice IAA-conjugating enzyme , was positively regulated by ARF8 . [ Sen. 6, subscore: 1.00 ]: Delivery of synthesized miR167 into cells led to decrease of both ARF8 mRNA and OsGH3-2 mRNA . [ Sen. 7, subscore: 1.00 ]: This study provides an evidence in which the exogeneous auxin signal is transduced to OsGH3-2 through miR167 and ARF8 in sequence .
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