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13 matches found in 4 documents. Search time: 0.009 seconds.
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Score: 6.00
Title: Functional roles of protein domains on rice alpha-amylase activity .
Author: Terashima M Hosono M Katoh S
Journal: Appl . Microbiol . Biotechnol . Citation: V : 47 ( 4 ) P : 364-7 Year: 1997 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub9163949 Accession (PMID): 9163949
Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: The enzymes that have an Amy1A-type N-terminal domain , Amy1A and Amy1A/3D , showed high activity against soluble starch , while the enzymes that have an Amy3D-type barrel structure , Amy3D and Amy1A/3D , showed high activity in oligosaccharide hydrolysis .
[ Sen. 1, subscore: 1.00 ]: Characteristics of two rice alpha-amylases Amy1A and Amy3D , and those of two chimeric enzymes Amy1A/3D and Amy3D/1A , engineered from the two isozymes , were compared in the light of the functional roles of protein domains in alpha-amylase .
[ Sen. 5, subscore: 1.00 ]: We have already characterized two rice alpha-amylase isozymes , Amy1A and Amy3D , and a chimeric enzyme engineered from these two isozymes , Amy1A/3D ( Terashima et al 1995 , 1996a , b ) .
[ Sen. 6, subscore: 1.00 ]: In spite of the high homology ( 70% ) of their amino acid sequences , Amy1A and Amy3D showed distinct differences in their enzymatic characteristics .
[ Sen. 7, subscore: 1.00 ]: The chimeric enzyme Amy1A/3D , which consists of an Amy1A-type N-terminal domain and an Amy3D-type barrel structure , inherited enzymatic characteristics from the both isozymes .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 4.00
Title: Characterization of rice alpha-amylase isozymes expressed by Saccharomyces cerevisiae .
Author: Terashima M Katoh S Thomas BR Rodriguez RL .
Journal: Appl . Microbiol . Biotechnol . Citation: V : 43 ( 6 ) P : 1050-5 Year: 1995 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub8590656 Accession (PMID): 8590656
Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: The pH optima for Amy3D ( pH optimum 5 . 5 ) and Amy1A ( pH optimum 4 . 2 ) correlate with the pH of the endosperm it issue at the times in rice seedling development when these isozymes are produced .
[ Sen. 5, subscore: 1.00 ]: Amy3D showed 10-14 times higher reactivity to oligosaccharides than Amy1A .
[ Sen. 6, subscore: 1.00 ]: Amy1A , on the other hand , showed higher reactivity to soluble starch and starch granules than Amy3D .
[ Sen. 7, subscore: 1.00 ]: These results suggest that the isozyme Amy3D , which is expressed at an early stage of germination , produces sugars from soluble starch during the early stage of seed germination and that the isozyme Amy1A works to initiate hydrolysis of the starch granules .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 3.00
Title: The roles of the N-linked carbohydrate chain of rice alpha-amylase in thermostability and enzyme kinetics .
Author: Terashima M Kubo A Suzawa M Itoh Y Katoh S
Journal: Eur . J Biochem . Citation: V : 226 ( 1 ) P : 249-54 Year: 1994 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub7957256 Accession (PMID): 7957256
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The thermostability and kinetics of starch hydrolysis were compared between a rice alpha-amylase isozyme Amy1A and its mutant enzyme that lacks an N-linked carbohydrate chain .
[ Sen. 2, subscore: 1.00 ]: Elimination of the N-glycosylation site in Amy1A reduced the thermostability of the enzyme .
[ Sen. 4, subscore: 1.00 ]: These results suggest that the N-linked carbohydrate chain of Amy1A has important roles in the thermostability and reaction kinetics of the enzyme .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: Differential expression of rice alpha-amylase genes during seedling development under anoxia .
Author: Hwang YS Thomas BR Rodriguez RL .
Journal: Plant Mol . Biol . Citation: V : 40 ( 6 ) P : 911-20 Year: 1999 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub10527416 Accession (PMID): 10527416
Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Anoxic conditions greatly diminished the expression of the Amy1A gene in aleurone .
Supplemental links/files: reference in endnote online text related articles pubmed citation
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