Score: 1.00 | | Author: Peng H Chun J Ai TB Tong YA Zhang R Zhao MM Chen F Wang SH | Journal: Plant Mol Biol Citation: V : 80 P : 85-102 Year: 2012 Type: In-Data-Review | Literature: oryza Field: abstract Doc ID: pub22403030 Accession (PMID): 22403030 | Abstract: Plant microRNAs ( miRNAs ) act as negative regulators of gene expression by slicing target transcripts or inhibiting translation .
A number of miRNAs play important roles in development .
In order to investigate the potential function of miRNAs during male gametogenesis in rice , we obtained both gene and small RNA expression profiles by combining microarray and high-throughput sequencing technologies .
From the microarray datasets , 2 , 925 male gametophyte-specific genes were identified , including 107 transcription factors and three significant Argonaute genes ( AGO12 , AGO13 , and AGO17 ) .
From the sRNA-Seq datasets , 104 unique miRNAs ( miRus ) were identified , including 47 known miRus and 57 novel miRus ; interestingly , most of the new miRus are pollen-specific and not conserved among species .
Furthermore , an interactive network of miRNA-target was constructed based on the two datasets .
By employing enrichment analysis , the miRNA-regulated targets were found to be involved in both the up and down pathways , but predominantly in the down pathways , including 37 GO biological processes and 32 KEGG pathways .
These findings indicate that miRNAs play a broad regulatory role during male gametophyte development in rice .
| Matching Sentences: [ Sen. 4, subscore: 1.00 ]: From the microarray datasets , 2 , 925 male gametophyte-specific genes were identified , including 107 transcription factors and three significant Argonaute genes ( AGO12 , AGO13 , and AGO17 ) .
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