Score: 9.00 |
|---|
| Title: Identification of a rice zinc finger protein whose expression is transiently induced by drought , cold but not by salinity and abscisic acid .
| | Author: Huang J Wang JF Wang QH Zhang HS .
| | Journal: DNA Seq .
Citation: V : 16 ( 2 ) P : 130-6 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16147864 Accession (PMID): 16147864 | | Abstract: A C2H2-type zinc finger protein gene ZFP245 was cloned by RT-PCR approach from cold treated rice seedlings .
ZFP245 is 712 bp in length and encodes a 24 . 5 KDa protein , which has 35% identity in amino acid with SCOF-1 , a cold-inducible zinc finger protein from soybean .
By database search , ZFP245 was mapped on chromosome 7 and clustered together with another C2H2 zinc finger gene .
Tissue expression analysis showed that ZFP245 was constitutively expressed in various rice it issues including roots , stems , leaves and spikes .
The semi-quantitative-RT-PCR assay revealed ZFP245 was strongly induced after 6 h exposure to cold and drought stresses , and then reduced to the baseline .
However , ZFP245 was not regulated by high salt or abscisic acid treatment .
The promoter sequence of 1017 nucleotides , upstream of ZFP245 , was cloned directly by PCR approach .
Sequence analysis revealed a CRT/DRE element was found within the ZFP245 promoter region .
Taken together , ZFP245 , as the first identified C2H2-type zinc finger protein involved in stress response in monocots probably plays a role as a transcription regulator in plant cold and drought responses through an ABA-independent pathway . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A C2H2-type zinc finger protein gene ZFP245 was cloned by RT-PCR approach from cold treated rice seedlings .
[ Sen. 2, subscore: 1.00 ]: ZFP245 is 712 bp in length and encodes a 24 . 5 KDa protein , which has 35% identity in amino acid with SCOF-1 , a cold-inducible zinc finger protein from soybean .
[ Sen. 3, subscore: 1.00 ]: By database search , ZFP245 was mapped on chromosome 7 and clustered together with another C2H2 zinc finger gene .
[ Sen. 4, subscore: 1.00 ]: Tissue expression analysis showed that ZFP245 was constitutively expressed in various rice it issues including roots , stems , leaves and spikes .
[ Sen. 5, subscore: 1.00 ]: The semi-quantitative-RT-PCR assay revealed ZFP245 was strongly induced after 6 h exposure to cold and drought stresses , and then reduced to the baseline .
[ Sen. 6, subscore: 1.00 ]: However , ZFP245 was not regulated by high salt or abscisic acid treatment .
[ Sen. 7, subscore: 1.00 ]: The promoter sequence of 1017 nucleotides , upstream of ZFP245 , was cloned directly by PCR approach .
[ Sen. 8, subscore: 1.00 ]: Sequence analysis revealed a CRT/DRE element was found within the ZFP245 promoter region .
[ Sen. 9, subscore: 1.00 ]: Taken together , ZFP245 , as the first identified C2H2-type zinc finger protein involved in stress response in monocots probably plays a role as a transcription regulator in plant cold and drought responses through an ABA-independent pathway .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
|---|
| Title: Increased tolerance of rice to cold , drought and oxidative stresses mediated by the overexpression of a gene that encodes the zinc finger protein ZFP245 .
| | Author: Huang J Sun SJ Xu DQ Yang X Bao YM Wang ZF Tang HJ Zhang H | | Journal: Biochem Biophys Res Commun Citation: V : 389 P : 556-61 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19751706 Accession (PMID): 19751706 | | Abstract: ZFP245 is a cold and drought-responsive gene that encodes a zinc finger protein in rice .
The ZFP245 protein localizes in the nucleus and exhibits trans-activation activity .
Transgenic rice plants overexpressing ZFP245 were generated and found to display high tolerance to cold and drought stresses .
The transgenic plants did not exhibit growth retardation , but showed growth sensitivity against exogenous abscisic acid , increased free proline levels and elevated expression of rice pyrroline-5-carboxylatesynthetase and proline transporter genes under stress conditions .
Overproduction of ZFP245 enhanced the activities of reactive oxygen species-scavenging enzymes under stress conditions and increased the tolerance of rice seedlings to oxidative stress .
Our data suggest that ZFP245 may contribute to the tolerance of rice plants to cold and drought stresses by regulating proline levels and reactive oxygen species-scavenging activities , and therefore may be useful for developing transgenic crops with enhanced tolerance to abiotic stress .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: ZFP245 is a cold and drought-responsive gene that encodes a zinc finger protein in rice .
[ Sen. 2, subscore: 1.00 ]: The ZFP245 protein localizes in the nucleus and exhibits trans-activation activity .
[ Sen. 3, subscore: 1.00 ]: Transgenic rice plants overexpressing ZFP245 were generated and found to display high tolerance to cold and drought stresses .
[ Sen. 5, subscore: 1.00 ]: Overproduction of ZFP245 enhanced the activities of reactive oxygen species-scavenging enzymes under stress conditions and increased the tolerance of rice seedlings to oxidative stress .
[ Sen. 6, subscore: 1.00 ]: Our data suggest that ZFP245 may contribute to the tolerance of rice plants to cold and drought stresses by regulating proline levels and reactive oxygen species-scavenging activities , and therefore may be useful for developing transgenic crops with enhanced tolerance to abiotic stress .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Increased tolerance of rice to cold , drought and oxidative stresses mediated by the overexpression of a gene that encodes the zinc finger protein ZFP245 .
| | Author: Huang J Sun SJ Xu DQ Yang X Bao YM Wang ZF Tang HJ Zhang H | | Journal: Biochem Biophys Res Commun Citation: V : 389 P : 556-61 Year: 2009 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub19751706 Accession (PMID): 19751706 | | Abstract: ZFP245 is a cold and drought-responsive gene that encodes a zinc finger protein in rice .
The ZFP245 protein localizes in the nucleus and exhibits trans-activation activity .
Transgenic rice plants overexpressing ZFP245 were generated and found to display high tolerance to cold and drought stresses .
The transgenic plants did not exhibit growth retardation , but showed growth sensitivity against exogenous abscisic acid , increased free proline levels and elevated expression of rice pyrroline-5-carboxylatesynthetase and proline transporter genes under stress conditions .
Overproduction of ZFP245 enhanced the activities of reactive oxygen species-scavenging enzymes under stress conditions and increased the tolerance of rice seedlings to oxidative stress .
Our data suggest that ZFP245 may contribute to the tolerance of rice plants to cold and drought stresses by regulating proline levels and reactive oxygen species-scavenging activities , and therefore may be useful for developing transgenic crops with enhanced tolerance to abiotic stress .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Increased tolerance of rice to cold , drought and oxidative stresses mediated by the overexpression of a gene that encodes the zinc finger protein ZFP245 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
