Score: 2.00 |
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| Title: Host strain specific sex pheromone variation in Spodoptera frugiperda .
| | Author: Groot AT Marr M Schofl G Lorenz S Svatos A Heckel DG | | Journal: Front Zool Citation: V : 5 P : 20 Year: 2008 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub19109878 Accession (PMID): 19109878 | | Abstract: ABSTRACT : BACKGROUND : The fall armyworm Spodoptera frugiperda ( Lepidoptera ; Noctuidae ) consists of two distinct strains with different host plant preferences for corn and rice .
To assess whether pheromonal-mediated behavioral isolation accompanies the habitat isolation on different host plants , we compared the sex pheromone composition among females of the two strains .
Pheromone glands were extracted with or without injection of pheromone biosynthesis activating neuropeptide ( PBAN ) .
To assess the mode of inheritance of this variation , we also analyzed the pheromone composition of F1 hybrid females .
RESULTS : Relative to intra-strain variation , the pheromone composition of the two strains differed significantly .
Corn strain females contained significantly more of the second most abundant pheromone compound Z11-16 : Ac ( m ) , and significantly less of most other compounds , than rice strain females .
When females were injected with PBAN before their glands were extracted , the differences between the strains were less pronounced but still statistically significant .
The pheromone composition of hybrid females showed a maternal inheritance of the major component Z9-14 : Ac ( M ) as well as of Z11-16 : Ac ( m ) .
Most other compounds showed an inheritance indicating genetic dominance of the corn strain .
The within-strain phenotypic correlations among the various components were consistent with their hypothesized biosynthetic pathway , and between-strain differences in the correlation structure suggested candidate genes that may explain the pheromone differences between the two strains .
These include Delta9 and Delta11 desaturases , and possibly also a Delta7-desaturase , although the latter has not been identified in insects so far .
CONCLUSION : The two host strains of S frugiperda produce systematically differing female sex pheromone blends .
Previously-documented geographic variation in the sexual communication of this species did not take strain identity into account , and thus may be partly explained by different strain occurrence in different regions .
The finding of pheromone differences reinforces the possibility of incipient reproductive isolation among these strains , previously shown to differ in the timing of nocturnal mating activity and host plant use .
Finding the genetic basis of the pheromone differences , as well as these other biological traits , will help to elucidate the role of premating isolation in the continuing differentiation of these two strains that may eventually lead to speciation .
| Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Corn strain females contained significantly more of the second most abundant pheromone compound Z11-16 : Ac ( m ) , and significantly less of most other compounds , than rice strain females .
[ Sen. 8, subscore: 1.00 ]: The pheromone composition of hybrid females showed a maternal inheritance of the major component Z9-14 : Ac ( M ) as well as of Z11-16 : Ac ( m ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Cloning , expression and functional analysis of a general odorant-binding protein 2 gene of the rice striped stem borer , Chilo suppressalis ( Walker ) ( Lepidoptera : Pyralidae ) .
| | Author: Gong ZJ Zhou WW Yu HZ Mao CG Zhang CX Cheng JA Zhu ZR | | Journal: Insect Mol Biol Citation: V : 18 P : 405-17 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19523072 Accession (PMID): 19523072 | | Abstract: A full-length cDNA encoding a general odorant binding protein 2 ( GOBP2 ) was cloned from the antennae of the rice striped stem borer , Chilo suppressalis ( Lepidoptera : Pyralidae ) , by the combination of reverse transcription PCR ( RT-PCR ) and rapid amplification of cDNA ends PCR ( RACE-PCR ) .
The cDNA contains a 489 bp open reading frame , which encodes a 162 amino acid protein , termed as Ch .
suppressalis GOBP2 ( CsupGOBP2 ) .
CsupGOBP2 is similar in the number of amino acids and protein sequence to GOBP2s in other species of Lepidoptera .
RT-PCR results showed that CsupGOBP2 mRNA was highly expressed in the adult antennae of both females and males , as was CsupGOBP2 protein as revealed by Western blot analysis .
CsupGOBP2 expressed in Escherichia coli was purified by affinity chromatography , refolding and gel filtration from the inclusion body .
Fluorescence emission spectra and competitive binding assays by using N-phenyl-1-naphthylamine as first binding ligand and odorants as potential competitors revealed that the CsupGOBP2 protein has significant affinity to cis-11-hexadecenal ( Z11-16 : Ald ) , the main component of Ch . suppressalis pheromone and to laurinaldehyd and benzaldehyde , two general plant volatile aldehydes .
| Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Fluorescence emission spectra and competitive binding assays by using N-phenyl-1-naphthylamine as first binding ligand and odorants as potential competitors revealed that the CsupGOBP2 protein has significant affinity to cis-11-hexadecenal ( Z11-16 : Ald ) , the main component of Ch . suppressalis pheromone and to laurinaldehyd and benzaldehyde , two general plant volatile aldehydes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
