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2 matches found in 2 documents. Search time: 0.023 seconds.
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Title: Identification and application of a rice senescence-associated promoter .
Author: Liu L Zhou Y Szczerba MW Li X Lin Y
Journal: Plant Physiol Citation: V : 153 P : 1239-49 Year: 2010 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20439547 Accession (PMID): 20439547
Abstract: SAG39 is a rice ( Oryza sativa ) gene that encodes a cysteine protease . SAG39 shares 55% homology with the Arabidopsis ( Arabidopsis thaliana ) senescence-associated protein SAG12 . The promoter for SAG39 ( P ( SAG39 ) ) was isolated , and SAG39 expression was determined to be relatively low in mature leaves , while not expressed in the endosperm . SAG39 mRNA levels increased as senescence progressed , with maximum accumulation of transcripts at late senescence stages . Gel retardation assays indicated that two cis-acting elements in P ( SAG39 ) , HBOXCONSENSUSPVCHS and WRKY71OS , responded to leaf senescence . To test if P ( SAG39 ) could be useful for increasing rice yields by increasing cytokinin content and delaying senescence , homozygous transgenic plants were obtained by linking P ( SAG39 ) to the ipt gene and introducing it into Zhonghua 11 . The chlorophyll level of the flag leaf was used to monitor senescence , confirming the stay-green phenotype in P ( SAG39 ) : ipt transgenic rice versus wild-type plants . Changes in the cytokinin content led to early flowering and a greater number of emerged panicles 70 d after germination in the transgenic rice . Measurements of sugar and nitrogen contents in flag leaves demonstrated a transition in the source-sink relationship in transgenic plants triggered at the onset of leaf senescence , with the nitrogen content decreasing more slowly , while sugars were removed more rapidly than in wild-type plants . The importance of these changes to rice physiology , yield , and early maturation will be discussed .
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[ Sen. 5, subscore: 1.00 ]: Gel retardation assays indicated that two cis-acting elements in P ( SAG39 ) , HBOXCONSENSUSPVCHS and WRKY71OS , responded to leaf senescence .
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Title: The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice .
Author: Zhang CQ Xu Y Lu Y Yu HX Gu MH Liu QQ
Journal: Planta Citation: V : 234 P : 541-54 Year: 2011 Type: In-Process
Literature: oryza Field: abstract Doc ID: pub21547461 Accession (PMID): 21547461
Abstract: WRKY proteins are a large super family of transcriptional regulators primarily involved in various plant physiological programs . In present study , the expression profile and putative function of the WRKY transcriptional factor , WRKY78 , in rice were identified . Real-time RT-PCR analysis showed that OsWRKY78 transcript was most abundant in elongating stems though its expression was detected in all the tested organs . The expression profiles were further confirmed by using promoter-GUS analysis in transgenic rice . OsWRKY78 : : GFP fusion gene transient expression analysis demonstrated that OsWRKY78 targeted to the nuclei of onion epidermal cell . Furthermore , OsWRKY78 RNAi and overexpression transgenic rice lines were generated . Transgenic plants with OsWRKY78 overexpression exhibited a phenotype identical to the wild type , whereas inhibition of OsWRKY78 expression resulted in a semi-dwarf and small kernel phenotype due to reduced cell length in transgenic plants . In addition , a T-DNA insertion mutant line oswrky78 was identified and a phenotype similar to that of RNAi plants was also observed . Grain quality analysis data showed no significant differences , with the exception of minor changes in endosperm starch crystal structure in RNAi plants . Taken together , these results suggest that OsWRKY78 may acts as a stem elongation and seed development regulator in rice .
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: In present study , the expression profile and putative function of the WRKY transcriptional factor , WRKY78 , in rice were identified .
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