| 23 matches found in 7 documents. Search time: 0.186 seconds. |
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Score: 12.00 |
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| Title: Rice WRKY45 plays important roles in fungal and bacterial disease resistance .
| | Author: Shimono M Koga H Akagi A Hayashi N Goto S Sawada M Kurihara T Matsushita A Sugano S Jiang CJ Kaku H Inoue H Takatsuji H | | Journal: Mol Plant Pathol Citation: V : 13 P : 83-94 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub21726399 Accession (PMID): 21726399 | | Abstract: Plant activators , such as benzothiadiazole ( BTH ) , protect plants from various diseases by priming the plant salicylic acid ( SA ) signalling pathway .
We have reported previously that a transcription factor identified in rice , WRKY45 ( OsWRKY45 ) , plays a pivotal role in BTH-induced disease resistance by mediating SA signalling .
Here , we report further functional characterization of WRKY45 .
Different plant activators vary in their action points , either downstream ( BTH and tiadinil ) or upstream ( probenazole ) of SA .
Rice resistance to Magnaporthe grisea , induced by both types of plant activator , was markedly reduced in WRKY45-knockdown ( WRKY45-kd ) rice , indicating a universal role for WRKY45 in chemical-induced resistance .
Fungal invasion into rice cells was blocked at most attempted invasion sites ( pre-invasive defence ) in WRKY45-overexpressing ( WRKY45-ox ) rice .
Hydrogen peroxide accumulated within the cell wall underneath invading fungus appressoria or between the cell wall and the cytoplasm , implying a possible role for H ( 2 ) O ( 2 ) in pre-invasive defence .
Moreover , a hypersensitive reaction-like reaction was observed in rice cells , in which fungal growth was inhibited after invasion ( post-invasive defence ) .
The two levels of defence mechanism appear to correspond to Type I and II nonhost resistances .
The leaf blast resistance of WRKY45-ox rice plants was much higher than that of other known blast-resistant varieties .
WRKY45-ox plants also showed strong panicle blast resistance .
BTH-induced resistance to Xanthomonas oryzae pv . oryzae was compromised in WRKY45-kd rice , whereas WRKY45-ox plants were highly resistant to this pathogen .
However , WRKY45-ox plants were susceptible to Rhizoctonia solani .
These results indicate the versatility and limitations of the application of this gene .
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[ Sen. 5, subscore: 3.00 ]: Rice resistance to Magnaporthe grisea , induced by both types of plant activator , was markedly reduced in WRKY45-knockdown ( WRKY45-kd ) rice , indicating a universal role for WRKY45 in chemical-induced resistance .
[ Sen. 6, subscore: 2.00 ]: Fungal invasion into rice cells was blocked at most attempted invasion sites ( pre-invasive defence ) in WRKY45-overexpressing ( WRKY45-ox ) rice .
[ Sen. 12, subscore: 2.00 ]: BTH-induced resistance to Xanthomonas oryzae pv . oryzae was compromised in WRKY45-kd rice , whereas WRKY45-ox plants were highly resistant to this pathogen .
[ Sen. 2, subscore: 1.00 ]: We have reported previously that a transcription factor identified in rice , WRKY45 ( OsWRKY45 ) , plays a pivotal role in BTH-induced disease resistance by mediating SA signalling .
[ Sen. 3, subscore: 1.00 ]: Here , we report further functional characterization of WRKY45 .
[ Sen. 10, subscore: 1.00 ]: The leaf blast resistance of WRKY45-ox rice plants was much higher than that of other known blast-resistant varieties .
[ Sen. 11, subscore: 1.00 ]: WRKY45-ox plants also showed strong panicle blast resistance .
[ Sen. 13, subscore: 1.00 ]: However , WRKY45-ox plants were susceptible to Rhizoctonia solani .
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Score: 10.00 |
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| Title: Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance .
| | Author: Shimono M Sugano S Nakayama A Jiang CJ Ono K Toki S Takatsuji H | | Journal: Plant Cell Citation: V : 19 P : 2064-76 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17601827 Accession (PMID): 17601827 | | Abstract: Benzothiadiazole ( BTH ) is a so-called plant activator and protects plants from diseases by activating the salicylic acid ( SA ) signaling pathway .
By microarray screening , we identified BTH and SA-inducible WRKY transcription factor ( TF ) genes that were upregulated within 3 h after BTH treatment .
Overexpression of one of them , WRKY45 , in rice ( Oryza sativa ) markedly enhanced resistance to rice blast fungus .
RNA interference-mediated knockdown of WRKY45 compromised BTH-inducible resistance to blast disease , indicating that it is essential for BTH-induced defense responses .
In a transient expression system , WRKY45 activated reporter gene transcription through W-boxes .
Epistasis analysis suggested that WRKY45 acts in the SA signaling pathway independently of NH1 , a rice ortholog of Arabidopsis thaliana NPR1 , which distinguishes WRKY45 from known Arabidopsis WRKY TFs .
Two defense-related genes , encoding a glutathione S-transferase and a cytochrome P450 , were found to be regulated downstream of WRKY45 but were not regulated by NH1 , consistent with the apparent independence of the WRKY45 and NH1-dependent pathways .
Defense gene expression in WRKY45-overexpressed rice plants varied with growth conditions , suggesting that some environmental factor ( s ) acts downstream of WRKY45 transcription .
We propose a role for WRKY45 in BTH-induced and SA-mediated defense signaling in rice and its potential utility in improving disease resistance of rice , an importance food resource worldwide .
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[ Sen. 6, subscore: 2.00 ]: Epistasis analysis suggested that WRKY45 acts in the SA signaling pathway independently of NH1 , a rice ortholog of Arabidopsis thaliana NPR1 , which distinguishes WRKY45 from known Arabidopsis WRKY TFs .
[ Sen. 7, subscore: 2.00 ]: Two defense-related genes , encoding a glutathione S-transferase and a cytochrome P450 , were found to be regulated downstream of WRKY45 but were not regulated by NH1 , consistent with the apparent independence of the WRKY45 and NH1-dependent pathways .
[ Sen. 8, subscore: 2.00 ]: Defense gene expression in WRKY45-overexpressed rice plants varied with growth conditions , suggesting that some environmental factor ( s ) acts downstream of WRKY45 transcription .
[ Sen. 3, subscore: 1.00 ]: Overexpression of one of them , WRKY45 , in rice ( Oryza sativa ) markedly enhanced resistance to rice blast fungus .
[ Sen. 4, subscore: 1.00 ]: RNA interference-mediated knockdown of WRKY45 compromised BTH-inducible resistance to blast disease , indicating that it is essential for BTH-induced defense responses .
[ Sen. 5, subscore: 1.00 ]: In a transient expression system , WRKY45 activated reporter gene transcription through W-boxes .
[ Sen. 9, subscore: 1.00 ]: We propose a role for WRKY45 in BTH-induced and SA-mediated defense signaling in rice and its potential utility in improving disease resistance of rice , an importance food resource worldwide .
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Score: 4.00 |
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| Title: Abscisic acid interacts antagonistically with salicylic acid signaling pathway in rice-Magnaporthe grisea interaction .
| | Author: Jiang CJ Shimono M Sugano S Kojima M Yazawa K Yoshida R Inoue H Hayashi N Sakakibara H Takatsuji H | | Journal: Mol Plant Microbe Interact Citation: V : 23 P : 791-8 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20459318 Accession (PMID): 20459318 | | Abstract: Plant hormones play pivotal signaling roles in plant-pathogen interactions .
Here , we report characterization of an antagonistic interaction of abscisic acid ( ABA ) with salicylic acid ( SA ) signaling pathways in the rice-Magnaporthe grisea interaction .
Exogenous application of ABA drastically compromised the rice resistance to both compatible and incompatible M grisea strains , indicating that ABA negatively regulates both basal and resistance gene-mediated blast resistance .
ABA markedly suppressed the transcriptional upregulation of WRKY45 and OsNPR1 , the two key components of the SA signaling pathway in rice , induced by SA or benzothiadiazole or by blast infection .
Overexpression of OsNPR1 or WRKY45 largely negated the enhancement of blast susceptibility by ABA , suggesting that ABA acts upstream of WRKY45 and OsNPR1 in the rice SA pathway .
ABA-responsive genes were induced during blast infection in a pattern reciprocal to those of WRKY45 and OsPR1b in the compatible rice-blast interaction but only marginally in the incompatible one .
These results suggest that the balance of SA and ABA signaling is an important determinant for the outcome of the rice-M grisea interaction .
ABA was detected in hyphae and conidia of M grisea as well as in culture media , implying that blast-fungus-derived ABA could play a role in triggering ABA signaling at host infection sites .
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[ Sen. 5, subscore: 2.00 ]: Overexpression of OsNPR1 or WRKY45 largely negated the enhancement of blast susceptibility by ABA , suggesting that ABA acts upstream of WRKY45 and OsNPR1 in the rice SA pathway .
[ Sen. 4, subscore: 1.00 ]: ABA markedly suppressed the transcriptional upregulation of WRKY45 and OsNPR1 , the two key components of the SA signaling pathway in rice , induced by SA or benzothiadiazole or by blast infection .
[ Sen. 6, subscore: 1.00 ]: ABA-responsive genes were induced during blast infection in a pattern reciprocal to those of WRKY45 and OsPR1b in the compatible rice-blast interaction but only marginally in the incompatible one .
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Score: 2.00 |
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| Title: Suppression of the rice fatty-acid desaturase gene OsSSI2 enhances resistance to blast and leaf blight diseases in rice .
| | Author: Jiang CJ Shimono M Maeda S Inoue H Mori M Hasegawa M Sugano S Takatsuji H | | Journal: Mol Plant Microbe Interact Citation: V : 22 P : 820-9 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19522564 Accession (PMID): 19522564 | | Abstract: Fatty acids and their derivatives play important signaling roles in plant defense responses .
It has been shown that suppressing a gene for stearoyl acyl carrier protein fatty-acid desaturase ( SACPD ) enhances the resistance of Arabidopsis ( SSI2 ) and soybean to multiple pathogens .
In this study , we present functional analyses of a rice homolog of SSI2 ( OsSSI2 ) in disease resistance of rice plants .
A transposon insertion mutation ( Osssi2-Tos17 ) and RNAi-mediated knockdown of OsSSI2 ( OsSSI2-kd ) reduced the oleic acid ( 18 : 1 ) level and increased that of stearic acid ( 18 : 0 ) , indicating that OsSSI2 is responsible for fatty-acid desaturase activity .
These plants displayed spontaneous lesion formation in leaf blades , retarded growth , slight increase in endogenous free salicylic acid ( SA ) levels , and SA/benzothiadiazole ( BTH ) -specific inducible genes , including WRKY45 , a key regulator of SA/BTH-induced resistance , in rice .
Moreover , the OsSSI2-kd plants showed markedly enhanced resistance to the blast fungus Magnaporthe grisea and leaf-blight bacteria Xanthomonas oryzae pv . oryzae .
These results suggest that OsSSI2 is involved in the negative regulation of defense responses in rice , as are its Arabidopsis and soybean counterparts .
Microarray analyses identified 406 genes that were differentially expressed ( >or=2-fold ) in OsSSI2-kd rice plants compared with wild-type rice and , of these , approximately 39% were BTH responsive .
Taken together , our results suggest that induction of SA-responsive genes , including WRKY45 , is likely responsible for enhanced disease resistance in OsSSI2-kd rice plants .
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[ Sen. 5, subscore: 1.00 ]: These plants displayed spontaneous lesion formation in leaf blades , retarded growth , slight increase in endogenous free salicylic acid ( SA ) levels , and SA/benzothiadiazole ( BTH ) -specific inducible genes , including WRKY45 , a key regulator of SA/BTH-induced resistance , in rice .
[ Sen. 9, subscore: 1.00 ]: Taken together , our results suggest that induction of SA-responsive genes , including WRKY45 , is likely responsible for enhanced disease resistance in OsSSI2-kd rice plants .
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Score: 1.00 |
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| Title: Isolation , fine mapping and expression profiling of a lesion mimic genotype , spl ( NF4050-8 ) that confers blast resistance in rice .
| | Author: Babu R Jiang CJ Xu X Kottapalli KR Takatsuji H Miyao A Hirochika H Kawasaki S | | Journal: Theor Appl Genet Citation: V : 122 P : 831-54 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21132425 Accession (PMID): 21132425 | | Abstract: We evaluated a large collection of Tos17 mutant panel lines for their reaction to three different races of Magnaporthe oryzae and identified a lesion mimic mutant , NF4050-8 , that showed lesions similar to naturally occurring spl5 mutant and enhanced resistance to all the three blast races tested .
Nested modified-AFLP using Tos17-specific primers and southern hybridization experiments of segregating individuals indicated that the lesion mimic phenotype in NF4050-8 is most likely due to a nucleotide change acquired during the culturing process and not due to Tos17 insertion per se .
Inheritance and genetic analyses in two japonica x indica populations identified an overlapping genomic region of 13 cM on short arm of chromosome 7 that was linked with the lesion mimic phenotype .
High-resolution genetic mapping using 950 F ( 3 ) and 3 , 821 F ( 4 ) plants of NF4050-8 x CO39 delimited a 35 kb region flanked by NBARC1 ( 5 . 262 Mb ) and RM8262 ( 5 . 297 Mb ) , which contained 6 ORFs ; 3 of them were resistance gene related with typical NBS-LRR signatures .
One of them harbored a NB-ARC domain , which had been previously demonstrated to be associated with cell death in animals .
Microarray analysis of NF4050-8 revealed significant up-regulation of numerous defense/pathogenesis-related genes and down-regulation of heme peroxidase genes .
Real-time PCR analysis of WRKY45 and PR1b genes suggested possible constitutive activation of a defense signaling pathway downstream of salicylic acid but independent of NH1 in these mutant lines of rice .
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[ Sen. 7, subscore: 1.00 ]: Real-time PCR analysis of WRKY45 and PR1b genes suggested possible constitutive activation of a defense signaling pathway downstream of salicylic acid but independent of NH1 in these mutant lines of rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
