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| Title: Methanol is an endogenous elicitor molecule for the synthesis of tryptophan and tryptophan-derived secondary metabolites upon senescence of detached rice leaves .
| | Author: Kang K Park S Natsagdorj U Kim YS Back K | | Journal: Plant J Citation: V : 66 P : 247-57 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21205035 Accession (PMID): 21205035 | | Abstract: During senescence of detached rice leaves , tryptophan ( Trp ) and Trp-derived secondary metabolites such as serotonin and 4-coumaroylserotonin accumulated in concert with methanol ( MeOH ) production .
This senescence-induced MeOH induction was closely associated with levels of pectin methylesterase ( PME ) 1 mRNA and PME enzyme activity .
Exogenous challenge of detached rice leaves with 1% MeOH accelerated Trp and serotonin biosynthesis with induction of the corresponding genes .
No other solvents , including ethanol , resulted in a Trp-inducing effect .
This MeOH-induced Trp synthesis was positively regulated by abscisic acid but negatively regulated by cytokinin , suggesting hormonal involvement in the action of MeOH .
Endogenous overproduction or suppression of MeOH either by PME1 overexpression or RNA interference ( RNAi ) gene silencing revealed that PME1 overexpressing lines produced twofold higher Trp levels with elevated Trp biosynthetic gene expression , whereas RNAi lines showed twofold reduction in Trp level in healthy control rice leaves , suggesting that MeOH acts as an endogenous elicitor to enhance Trp biosynthesis .
Among many transcription factors induced following MeOH treatment , the WRKY family showed significant induction patterns , of which WRKY14 appeared to play a key regulatory role in MeOH-induced Trp and Trp-derived secondary metabolite biosynthesis .
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[ Sen. 7, subscore: 1.00 ]: Among many transcription factors induced following MeOH treatment , the WRKY family showed significant induction patterns , of which WRKY14 appeared to play a key regulatory role in MeOH-induced Trp and Trp-derived secondary metabolite biosynthesis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
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| Title: Comprehensive sequence and whole-life-cycle expression profile analysis of the phosphate transporter gene family in rice .
| | Author: Liu F Chang XJ Ye Y Xie WB Wu P Lian XM | | Journal: Mol Plant Citation: V : 4 P : 1105-22 Year: 2011 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub21832284 Accession (PMID): 21832284 | | Abstract: Plant phosphate transporter ( PT ) genes comprise a large family with important roles in various physiological and biochemical processes .
In this study , a database search yielded 26 potential PT family genes in rice ( Oryza sativa ) .
Analysis of these genes led to identification of eight conserved motifs and 5-12 trans-membrane segments , most of them conserved .
A total of 237 putative cis elements were found in the 2-kb upstream region of these genes .
Of these , a majority were Pi-response and other stress-related cis regulatory elements , such as PHO-like , TATA-box-like , PHR1 , or Helix-loop-helix elements , and WRKY1 and ABRE elements , suggesting gene regulation by these signals .
Comprehensive expression analysis of these genes was performed using data from microarrays hybridized with RNA from 27 it issues covering the entire lifecycle from three rice genotypes : Minghui 63 , Zhenshan 97 , and Shanyou 63 .
Real-time PCR analysis confirmed that three rice PT genes are preferentially expressed in stamen at 1 d before flowering , two in panicle at the heading stage , and two in flag leaf at 14 d after the heading stage .
Hormone-treatment experiments revealed differential up-regulation or down-regulation of 11 rice PT genes in seedlings exposed to five hormones , respectively .
These results will be useful for elucidating the roles of these genes in the growth , development , and stress response of the rice plant .
| Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Of these , a majority were Pi-response and other stress-related cis regulatory elements , such as PHO-like , TATA-box-like , PHR1 , or Helix-loop-helix elements , and WRKY1 and ABRE elements , suggesting gene regulation by these signals .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
