| 7 matches found in 5 documents. Search time: 0.129 seconds. |
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Score: 4.00 |
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| Title: Mechanism of resistance to carpropamid in Magnaporthe grisea .
| | Author: Takagaki M Kaku K Watanabe S Kawai K Shimizu T Sawada H Kumakura K Nagayama K | | Journal: Pest Manag . Sci . Citation: V : 60 ( 9 ) P : 921-6 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15382507 Accession (PMID): 15382507 | | Abstract: The inhibitory activity of carpropamid on scytalone dehydratase ( SDH ) extracted from a carpropamid-resistant strain of Magnaporthe grisea ( Hebert ) Barr was dramatically reduced in comparison with that on SDH extracted from the sensitive strain .
A single-point mutation ( G to A ) located at the upstream region ( 233 bp downstream from the ATG codon ) resulting in a one-amino-acid substitution ( valine [ GTG ] 75 to methionine [ ATG ] : V75M ) was found in the resistant strain .
To examine whether the V75M mutation is the primary reason for decreasing the sensitivity of SDH to carpropamid , the SDH cDNAs of both the sensitive and the resistant strain were cloned into a GST-fused protein expression vector-system .
The recombinant SDHs of both strains exhibited the same sensitivities to carpropamid as those extracted from the mycelia of the respective strains .
These data clearly revealed that the V75M mutation causes the low sensitivities of the SDHs of the carpropamid-resistant strains , and strongly suggests that the V75M mutation confers resistance of these strains to carpropamid . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: These data clearly revealed that the V75M mutation causes the low sensitivities of the SDHs of the carpropamid-resistant strains , and strongly suggests that the V75M mutation confers resistance of these strains to carpropamid .
[ Sen. 2, subscore: 1.00 ]: A single-point mutation ( G to A ) located at the upstream region ( 233 bp downstream from the ATG codon ) resulting in a one-amino-acid substitution ( valine [ GTG ] 75 to methionine [ ATG ] : V75M ) was found in the resistant strain .
[ Sen. 3, subscore: 1.00 ]: To examine whether the V75M mutation is the primary reason for decreasing the sensitivity of SDH to carpropamid , the SDH cDNAs of both the sensitive and the resistant strain were cloned into a GST-fused protein expression vector-system .
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Score: 2.00 |
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| Title: [ Trichothecenes in staple food from a high-incidence area of carcinoma of the esophagus and gastric cardia and their carcinogenic potential ] | | Author: Xia QJ Lu XQ Wu JL Li YS .
| | Journal: Zhonghua Zhong Liu Za Zhi Citation: V : 10 ( 1 ) P : 4-8 Year: 1988 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub2970952 Accession (PMID): 2970952 | | Abstract: This is the first report to demonstrate the presence of a group of mycotoxins in corn-staple food of Linxian residents , including Nivalenoal ( NIV ) , Deoxynivalenol ( DON ) , 3-acetyl-deoxynivalenol ( 3-ADON ) , 15-acetyl-deoxynivalenol ( 15-ADON ) , T-2 toxin ( these 5 toxins belong to trichothecenes ) and zearalenone ( ZEN ) .
These 6 toxins were produced by Fusarium species .
Using TLC , HPLC , GC , monoclonal antibody affinity column chromatography combined with RIA , respectively , 107 corn samples from Linxian and 2 rice samples from USA were analyzed .
It was demonstrated that the average level of NIV was 757 +/- 707 ( 54-2 , 760 ) ng/g , DON was 5 , 376 +/- 4 , 460 ( 360-12 , 670 ) ng/g with 100% positivity in corn samples consumed as staple food by esophageal cancer patients and their families .
Corn samples collected from 5 villages in Linxian at different seasons in 1984-1986 all showed high levels of NIV and DON with 100% positivity which suggested that NIV and DON were consistently and widely existed in Linxian corn samples .
Levels of 3-ADON and 15-ADON in Linxian corn were 113 +/- 57 and 495 +/- 538 ng/g .
This paper also first demonstrates that the extracts of corn from esophageal cancer patients families and their NIV and DON fractions purified by HPLC could induce significant chromosome aberrations in V79 cells , indicating that they had carcinogenic potential Pure toxins of NIV , DON , T-2 and 3-ADON could induce chromosome aberrations in V79 cells starting from 1 ng/ml-a very low concentration .
When concentrations increased to certain range they all showed toxic effects . ( ABSTRACT TRUNCATED AT 250 WORDS ) | Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: This paper also first demonstrates that the extracts of corn from esophageal cancer patients families and their NIV and DON fractions purified by HPLC could induce significant chromosome aberrations in V79 cells , indicating that they had carcinogenic potential Pure toxins of NIV , DON , T-2 and 3-ADON could induce chromosome aberrations in V79 cells starting from 1 ng/ml-a very low concentration .
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Score: 1.00 |
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| Title: Antioxidative , antimutagenic , and anticarcinogenic activities of rice bran extracts in chemical and cell assays .
| | Author: Nam SH Choi SP Kang MY Kozukue N Friedman M | | Journal: J Agric .
Food Chem .
Citation: V : 53 ( 3 ) P : 816-22 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15686439 Accession (PMID): 15686439 | | Abstract: Ethanol-water ( 70 : 30 v/v ) extracts from rice brans removed from seeds of two blackish-purple pigmented ( Sanhaehyanghyulla and Suwon 415 ) and one nonpigmented ( Chuchung ) brown rice cultivars were evaluated for antioxidative , anti-tumor-promoting , and anticarcinogenic activities in chemical assays and in mammalian cells ( human leukemia HL-60 , marmoset B lymphoblastoid B95-8 , and Chinese hamster V79 lung cells ) by the following tests : inhibition of xanthine oxidase activity ; chelation of ferrous ions ; reduction of potassium ferricyanide ; scavenging of superoxide anions , hydroxyl radicals , and intracellular peroxides ; inhibition of 4-nitroquinoline N-oxide-induced mutagenesis ; and inhibition of phorbol ester-induced tumor promotion .
The extracts from the pigmented rice seeds had generally higher activities in all tests than did the extract from the nonpigmented variety .
The results suggest that brans from pigmented rice varieties may provide a source of new natural antioxidants and anticarcinogens and that such rice cultivars with high antioxidative potential also provide a genetic resource for the development of new , improved rice cultivars that may make it possible to enhance both the nutritional and medical value of rice-based diets . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Ethanol-water ( 70 : 30 v/v ) extracts from rice brans removed from seeds of two blackish-purple pigmented ( Sanhaehyanghyulla and Suwon 415 ) and one nonpigmented ( Chuchung ) brown rice cultivars were evaluated for antioxidative , anti-tumor-promoting , and anticarcinogenic activities in chemical assays and in mammalian cells ( human leukemia HL-60 , marmoset B lymphoblastoid B95-8 , and Chinese hamster V79 lung cells ) by the following tests : inhibition of xanthine oxidase activity ; chelation of ferrous ions ; reduction of potassium ferricyanide ; scavenging of superoxide anions , hydroxyl radicals , and intracellular peroxides ; inhibition of 4-nitroquinoline N-oxide-induced mutagenesis ; and inhibition of phorbol ester-induced tumor promotion .
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Score: 1.00 |
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| Title: Antioxidative and antimutagenic activities of 70% ethanolic extracts from four fungal mycelia-fermented specialty rices .
| | Author: Ra Yoon M Hyun Nam S Young Kang M | | Journal: J Clin Biochem Nutr Citation: V : 43 P : 118-25 Year: 2008 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub18818745 Accession (PMID): 18818745 | | Abstract: The health-promoting potential of 70% ethanolic extracts of 4 rice varieties fermented with Monascus ruber , Phellinus linteus , Cordyceps sinensis and Agaricus blazei was evaluated mainly focusing on their antioxidative and antimutagenic capacities based on the following parameters : phenolic compound and phytic acid content ; inhibitory activity on lipid peroxidation ; scavenging activity on DPPH radical ; suppressing ability on mitomycin C-induced mutagenesis in E coli cells ; and protective effect on 4-nitroquinoline oxide-triggered DNA lesion in V79 hamster cells .
The fermented rice extracts were superior in overall health-promoting parameters compared to the source material The higher antimutagenic activity of the fermented rice extracts might be in part caused by a larger amount of antioxidant constituents such as phenolic compounds or phytic acid .
Of the fungal species , Monascus ruber was found to impart a marked increase in both the antioxidative and antimutagenic abilities to the source material The current study suggests a possibility that such fermented rice may contribute to the prevention of lifestyle-related diseases such as cancer through a daily intake of rice-based diets .
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[ Sen. 1, subscore: 1.00 ]: The health-promoting potential of 70% ethanolic extracts of 4 rice varieties fermented with Monascus ruber , Phellinus linteus , Cordyceps sinensis and Agaricus blazei was evaluated mainly focusing on their antioxidative and antimutagenic capacities based on the following parameters : phenolic compound and phytic acid content ; inhibitory activity on lipid peroxidation ; scavenging activity on DPPH radical ; suppressing ability on mitomycin C-induced mutagenesis in E coli cells ; and protective effect on 4-nitroquinoline oxide-triggered DNA lesion in V79 hamster cells .
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Score: 1.00 |
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| Title: Effect of sodium azide on sister-chromatid exchanges in human lymphocytes and Chinese hamster cells .
| | Author: Arenaz P Nilan RA .
| | Journal: Mutat . Res . Citation: V : 88 ( 2 ) P : 217-21 Year: 1981 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub7219439 Accession (PMID): 7219439 | | Abstract: Previous reports from this laboratory and others indicate that sodium azide is a unique mutagen .
It is highly mutagenic in S typhimurium TA1530 as well as in barley , rice , peas , yeast and Chinese hamster V79 cells .
However , azide apparently does not produce chromosome breaks in barley , Vicia or human lymphocytes .
Therefore , a study of the effects of azide on sister-chromatid exchanges ( SCE ) appeared warranted .
Human whole blood and Chinese hamster K1 cell line were exposed for 4 and 2 h resp . to various concentrations of sodium azide ranging from 10 ( -3 ) to 10 ( -7 ) M Cells were harvested and chromosomes stained by the FPG technique .
In human lymphocytes , concentrations above 10 ( -4 ) induced lethality whereas the K1 cell line was sensitive to concentrations above 10 ( -5 ) M The lower concentrations of azide produced no significant increase in SCE frequency above controls .
Concurrent mitomycin C treatments produced significant increases in SCE levels .
This apparent lack of induction of SCEs above background combined with previous data demonstrating negative clastogenic but very positive mutagenic activity of azide confirms the uniqueness of this mutagen .
It would appear that azide is one of the few known potent mutagens that does not increase SCEs and/or break chromosomes .
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[ Sen. 2, subscore: 1.00 ]: It is highly mutagenic in S typhimurium TA1530 as well as in barley , rice , peas , yeast and Chinese hamster V79 cells .
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