| 10 matches found in 7 documents. Search time: 0.208 seconds. |
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| Title: Mutagenesis study of rice nonspecific lipid transfer protein 2 reveals residues that contribute to structure and ligand binding .
| | Author: Cheng CS Chen MN Lai YT Chen T Lin KF Liu YJ Lyu PC | | Journal: Proteins Citation: V : 70 P : 695-706 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17729272 Accession (PMID): 17729272 | | Abstract: Plant nonspecific lipid transfer protein 2 ( nsLTP2 ) is a small ( 7 kDa ) protein that binds lipid-like ligands .
An inner hydrophobic cavity surrounded by alpha-helices is the defining structural feature of nsLTP2 .
Although nsLTP2 structures have been reported earlier , the detailed mechanisms of ligand binding and lipid transfer remain unclear .
In this study , we used site-directed mutagenesis to determine the role of various hydrophobic residues ( L8 , I15 , F36 , F39 , Y45 , Y48 , and V49 ) in the structure , stability , ligand binding , and lipid transfer activity of rice nsLTP2 .
Three single mutations ( L8A , F36A , and V49A ) drastically alter the native tertiary structure and perturb ligand binding and lipid transfer activity .
Therefore , these three residues are structurally important .
The Y45A mutant , however , retains a native-like structure but has decreased lipid binding affinity and lipid transfer activity , implying that this aromatic residue is critical for these biological functions .
The mutants , I15A and Y48A , exhibit quite different ligand binding affinities .
Y48 is involved in planar sterol binding but not linear lysophospholipid association .
As for I15A , it had the highest dehydroergosterol binding affinity in spite of the lower lipid binding and transfer abilities .
Our results suggest that the long alkyl side chain of I15 would restrict the flexibility of loop I ( G13-A19 ) for sterol entry .
Finally , F39A can markedly increase the exposed hydrophobic surface to maintain its transfer efficiency despite reduced ligand binding affinity .
These findings suggest that the residues forming the hydrophobic cavity play various important roles in the structure and function of rice nsLTP2 .
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[ Sen. 4, subscore: 1.00 ]: In this study , we used site-directed mutagenesis to determine the role of various hydrophobic residues ( L8 , I15 , F36 , F39 , Y45 , Y48 , and V49 ) in the structure , stability , ligand binding , and lipid transfer activity of rice nsLTP2 .
[ Sen. 5, subscore: 1.00 ]: Three single mutations ( L8A , F36A , and V49A ) drastically alter the native tertiary structure and perturb ligand binding and lipid transfer activity .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Oral vaccination of chickens against Newcastle disease with V4 vaccine delivered on processed rice grains .
| | Author: Jayawardane GW De Alwis MC Bandara D | | Journal: Aust Vet .
J Citation: V : 67 ( 10 ) P : 364-6 Year: 1990 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub2288540 Accession (PMID): 2288540 | | Abstract: An international effort ( sponsored by the Australian Centre for International Agricultural Research ) is being made to develop oral vaccines that will protect village chickens against Newcastle disease .
The vaccines being used are derivatives of the avirulent Australian V4 strain that have been selected for enhanced heat resistance .
The present study , undertaken in Sri Lanka , used local processed ( parboiled ) rice as a vehicle for the vaccine .
Chickens receiving two doses of vaccine on cooked , parboiled rice were completely protected against contact challenge with the virulent SL 88/1 Sri Lankan strain of Newcastle disease virus Chickens kept in contact with these vaccinated chickens were similarly protected .
Lower levels of protection were achieved with vaccine given on uncooked parboiled rice .
V4 vaccine administered intranasally also gave complete protection .
Serums from vaccinated chickens that survived challenge were tested for haemagglutination-inhibition antibodies , using both vaccine virus and challenge virus as antigens .
Titres were higher against vaccine virus . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The vaccines being used are derivatives of the avirulent Australian V4 strain that have been selected for enhanced heat resistance .
[ Sen. 6, subscore: 1.00 ]: V4 vaccine administered intranasally also gave complete protection .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: The contribution of lectins to the interaction between oral Newcastle disease vaccine and grains .
| | Author: Rehmani SF Spradbrow PB .
| | Journal: Vet . Microbiol . Citation: V : 46 ( 1-3 ) P : 55-62 Year: 1995 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8545979 Accession (PMID): 8545979 | | Abstract: Successful oral vaccination of chickens with Newcastle disease ( ND ) depends on the survival of vaccine virus on the grains that are used as carriers .
Some interactions between grains and the V4 strain of ND virus ( NDV ) were studied .
Crude saline washings were prepared from several grains - rice ( unhusked , brown , white and boiled white ) , sorghum , millet , wheat , maize and barley - and tested for lectin activity , as indicated by agglutination of chicken erythrocytes .
Only washings from unhusked rice , sorghum and millet failed to haemagglutinate .
None of the crude washings antagonised the haemagglutinating activity of NDV , and the washing from white rice produced an 8-fold enhancement .
The presence of lectins in the washings from rice , wheat and barley was confirmed by purifying a substance with N-acetyl-D-glucosamine specificity .
Only the crude extract from white rice had any profound effect on infectivity , reducing the infectivity titre by 99 . 99% .
It is not known if the viricidal substance is identical with the lectin .
Of 9 commercial lectins tested , only ConA bound the V4 virus .
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[ Sen. 2, subscore: 1.00 ]: Some interactions between grains and the V4 strain of ND virus ( NDV ) were studied .
[ Sen. 9, subscore: 1.00 ]: Of 9 commercial lectins tested , only ConA bound the V4 virus .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Characterization of archaeal community in contaminated and uncontaminated surface stream sediments .
| | Author: Porat I Vishnivetskaya TA Mosher JJ Brandt CC Yang ZK Brooks SC Liang L Drake MM Podar M Brown SD Palumbo AV | | Journal: Microb Ecol Citation: V : 60 P : 784-95 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20725722 Accession (PMID): 20725722 | | Abstract: Archaeal communities from mercury and uranium-contaminated freshwater stream sediments were characterized and compared to archaeal communities present in an uncontaminated stream located in the vicinity of Oak Ridge , TN , USA .
The distribution of the Archaea was determined by pyrosequencing analysis of the V4 region of 16S rRNA amplified from 12 streambed surface sediments .
Crenarchaeota comprised 76% of the 1 , 670 archaeal sequences and the remaining 24% were from Euryarchaeota .
Phylogenetic analysis further classified the Crenarchaeota as a Freshwater Group , Miscellaneous Crenarchaeota group , Group I3 , Rice Cluster VI and IV , Marine Group I and Marine Benthic Group B ; and the Euryarchaeota into Methanomicrobiales , Methanosarcinales , Methanobacteriales , Rice Cluster III , Marine Benthic Group D , Deep Sea Hydrothermal Vent Euryarchaeota 1 and Eury 5 .
All groups were previously described .
Both hydrogen and acetate-dependent methanogens were found in all samples .
Most of the groups ( with 60% of the sequences ) described in this study were not similar to any cultivated isolates , making it difficult to discern their function in the freshwater microbial community .
A significant decrease in the number of sequences , as well as in the diversity of archaeal communities was found in the contaminated sites .
The Marine Group I , including the ammonia oxidizer Nitrosopumilus maritimus , was the dominant group in both mercury and uranium/nitrate-contaminated sites .
The uranium-contaminated site also contained a high concentration of nitrate , thus Marine Group I may play a role in nitrogen cycle .
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[ Sen. 2, subscore: 1.00 ]: The distribution of the Archaea was determined by pyrosequencing analysis of the V4 region of 16S rRNA amplified from 12 streambed surface sediments .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Oral vaccination of chickens with the V4 strain of Newcastle disease virus .
Cooked and raw white rice as a vehicle .
| | Author: Samuel JL Bensink Z Spradbrow PB .
| | Journal: Citation: V : 25 ( 1 ) P : 2-10 Year: 1993 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8465439 Accession (PMID): 8465439 | | Abstract: Uncooked white rice and cooked white rice were tested as vehicles for the V4 strain of oral Newcastle disease vaccine .
The results of feeding experiments were evaluated by the measurement of haemagglutination inhibition antibodies against Newcastle disease virus .
Little of the virus applied to uncooked white rice could be recovered , even immediately after mixing , whereas when the virus was applied to cooked white rice most of it could be recovered .
In 4 separate experiments , chickens failed to respond serologically to vaccine supplied on uncooked white rice .
In all of 4 experiments with cooked white rice , there were serological responses in vaccinated chickens , from 45% to 100% of the chickens developing titres sufficiently high to indicate protection against challenge with virulent virus .
Development of haemagglutination inhibition antibodies in some control chickens indicated the ability of the vaccine virus for lateral spread or persistence in the environment . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Uncooked white rice and cooked white rice were tested as vehicles for the V4 strain of oral Newcastle disease vaccine .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
