| 12 matches found in 5 documents. Search time: 0.349 seconds. |
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Score: 6.00 |
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| Title: Rice Undeveloped Tapetum1 is a major regulator of early tapetum development .
| | Author: Jung KH Han MJ Lee YS Kim YW Hwang I Kim MJ Kim YK Nahm BH An G | | Journal: Plant Cell Citation: V : 17 ( 10 ) P : 2705-22 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16141453 Accession (PMID): 16141453 | | Abstract: The tapetum , the innermost of four sporophytic layers in the anther wall , comes in direct contact with the developing male gametophyte and is thought to play a crucial role in the development and maturation of microspores .
Here , we report the identification of rice ( Oryza sativa ) Undeveloped Tapetum1 ( Udt1 ) , which is required for the differentiation of secondary parietal cells to mature tapetal cells .
T-DNA or retrotransposon Tos17 insertions in the Udt1 gene caused male sterility .
The anther walls and meiocytes of the mutants were normal during the early premeiosis stage , but their tapeta failed to differentiate and became vacuolated during the meiotic stage .
In addition , meiocytes did not develop to microspores , and middle layer degeneration was inhibited .
Consequently , the anther locules contained no pollen .
The UDT1 : green fluorescent protein fusion protein was localized to the nucleus .
This , together with its homology with other basic helix-loop-helix proteins , suggests that UDT1 is a transcription factor .
DNA microarray analysis identified 958 downregulated and 267 upregulated genes in the udt1-1 anthers , suggesting that Udt1 plays a major role in maintaining tapetum development , starting in early meiosis . | Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: DNA microarray analysis identified 958 downregulated and 267 upregulated genes in the udt1-1 anthers , suggesting that Udt1 plays a major role in maintaining tapetum development , starting in early meiosis .
[ Sen. 2, subscore: 1.00 ]: Here , we report the identification of rice ( Oryza sativa ) Undeveloped Tapetum1 ( Udt1 ) , which is required for the differentiation of secondary parietal cells to mature tapetal cells .
[ Sen. 3, subscore: 1.00 ]: T-DNA or retrotransposon Tos17 insertions in the Udt1 gene caused male sterility .
[ Sen. 7, subscore: 1.00 ]: The UDT1 : green fluorescent protein fusion protein was localized to the nucleus .
[ Sen. 8, subscore: 1.00 ]: This , together with its homology with other basic helix-loop-helix proteins , suggests that UDT1 is a transcription factor .
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Score: 5.00 |
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| Title: Identification of gamyb-4 and analysis of the regulatory role of GAMYB in rice anther development .
| | Author: Liu Z Bao W Liang W Yin J Zhang D | | Journal: J Integr Plant Biol Citation: V : 52 P : 670-8 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20590996 Accession (PMID): 20590996 | | Abstract: In higher plants , male reproductive development is a complex biological process that includes cell division and differentiation , cell to cell communication etc , while the mechanism underlying plant male reproductive development remains less understood .
GAMYB encodes a gibberellins acid ( GA ) inducible transcription factor that is required for the early anther development in rice ( Oryza sativa L ) .
Here , we report the isolation and characterization of a new allele gamyb-4 with a C base deletion in the second exon ( +2308 ) , causing a frame shift and premature translational termination .
Histological analysis showed that gamyb-4 developed abnormal enlarged tapetum and could not undergo normal meiosis .
To understand the regulatory role of GAMYB , we carried out quantitative reverse transcription-polymerase chain reaction analysis and comparison of microarray data .
These results revealed that the expression of TDR ( TAPETUM DEGENERATION RETARDATION ) , a tapetal cell death regulator , was downregulated in gamyb-4 and udt1 ( undeveloped tapetum1 ) .
While the GAMYB expression was not obviously changed in tdr and udt1-1 , and no apparent expression fold change of UDT1 in tdr and gamyb-4 , suggesting that TDR may act downstream of GAMYB and UDT1 , and GAMYB and UDT1 work in parallel to regulate rice early anther development .
This work is helpful in understanding the regulatory network in rice anther development .
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[ Sen. 7, subscore: 4.00 ]: While the GAMYB expression was not obviously changed in tdr and udt1-1 , and no apparent expression fold change of UDT1 in tdr and gamyb-4 , suggesting that TDR may act downstream of GAMYB and UDT1 , and GAMYB and UDT1 work in parallel to regulate rice early anther development .
[ Sen. 6, subscore: 1.00 ]: These results revealed that the expression of TDR ( TAPETUM DEGENERATION RETARDATION ) , a tapetal cell death regulator , was downregulated in gamyb-4 and udt1 ( undeveloped tapetum1 ) .
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Score: 3.00 |
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| Title: The rice gene DEFECTIVE TAPETUM AND MEIOCYTES 1 ( DTM1 ) is required for early tapetum development and meiosis .
| | Author: Yi J Kim SR Lee DY Moon S Lee YS Jung KH Hwang I An G | | Journal: Plant J Citation: V : 70 P : 256-70 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22111585 Accession (PMID): 22111585 | | Abstract: Tapetum development and meiosis play crucial roles in anther development .
Here we identified a rice gene , DEFECTIVE TAPETUM AND MEIOCYTES 1 ( DTM1 ) , which controls the early stages of that development .
This gene encodes for an endoplasmic reticulum ( ER ) membrane protein that is present only in cereals .
Our T-DNA insertion mutations gave rise to abnormal tapetal formation .
Cellular organelles , especially the ER , were underdeveloped , which led to hampered differentiation and degeneration of the tapetum .
In addition , the development of pollen mother cells was arrested at the early stages of meiotic prophase I RNA in-situ hybridization analyses showed that DTM1 transcripts were most abundant in tapetal cells at stages 6 and 7 , and moderately in the pollen mother cells and meiocytes .
Transcripts of UDT1 , which functions in tapetum development during early meiosis , were reduced in dtm1 anthers , as were those of PAIR1 , which is involved in chromosome pairing and synapsis during meiosis .
However , expression of MSP1 and MEL1 , which function in anther wall specification and germ cell division , respectively , was not altered in the dtm1 mutant .
Moreover , transcripts of DTM1 were reduced in msp1 mutant anthers , but not in udt1 and pair1 mutants .
These results , together with their mutant phenotypes , suggest that DTM1 plays important roles in the ER membrane during early tapetum development , functioning after MSP1 and before UDT1 , and also in meiocyte development , after MEL1 and before PAIR1 .
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[ Sen. 7, subscore: 1.00 ]: Transcripts of UDT1 , which functions in tapetum development during early meiosis , were reduced in dtm1 anthers , as were those of PAIR1 , which is involved in chromosome pairing and synapsis during meiosis .
[ Sen. 9, subscore: 1.00 ]: Moreover , transcripts of DTM1 were reduced in msp1 mutant anthers , but not in udt1 and pair1 mutants .
[ Sen. 10, subscore: 1.00 ]: These results , together with their mutant phenotypes , suggest that DTM1 plays important roles in the ER membrane during early tapetum development , functioning after MSP1 and before UDT1 , and also in meiocyte development , after MEL1 and before PAIR1 .
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Score: 2.00 |
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| Title: [ Identification of the rice ( Oryza sativa L ) mutant msp1-4 and expression analysis of its UDT1 and GAMYB genes ] | | Author: Wang Y Wang YF Zhang DB .
| | Journal: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao Citation: V : 32 ( 5 ) P : 527-34 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17075175 Accession (PMID): 17075175 | | Abstract: A rice male-sterile mutant msp1-4 ( MULTIPLE SPOROCYTE ) with japonica cultivar 9522 background , was obtained in M ( 3 ) population treated with ( 60 ) Co gamma-ray .
Results of genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive locus .
To map this locus , an F ( 2 ) population was constructed from the cross between the msp1-4 ( japonica ) and LongTeFu B ( indica ) .
This locus was mapped between the two InDel markers , WY-4 and WY-8 , with physical distance of 247 kb .
A deletion with 10 base pairs between 758 bp and 767 bp in MSP1 open reading frame was confirmed by sequence analysis , which led to pre-termination of MSP1 translation .
Phenotype analysis of msp1-4 indicated that it was similar to the msp1 mutant .
To insight the expression change of rice anther developmental genes in this mutant , semi-quantitative RT-PCR analysis was carried out .
The results showed that the expression level of rice UDT1 and GAMYB were reduced in msp1-4 , implying that UDT1 and GAMYB are possibly the downstream genes of MSP1 gene in rice pollen development . | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: The results showed that the expression level of rice UDT1 and GAMYB were reduced in msp1-4 , implying that UDT1 and GAMYB are possibly the downstream genes of MSP1 gene in rice pollen development .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: [ Identification of the rice ( Oryza sativa L ) mutant msp1-4 and expression analysis of its UDT1 and GAMYB genes ] | | Author: Wang Y Wang YF Zhang DB .
| | Journal: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao Citation: V : 32 ( 5 ) P : 527-34 Year: 2006 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub17075175 Accession (PMID): 17075175 | | Abstract: A rice male-sterile mutant msp1-4 ( MULTIPLE SPOROCYTE ) with japonica cultivar 9522 background , was obtained in M ( 3 ) population treated with ( 60 ) Co gamma-ray .
Results of genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive locus .
To map this locus , an F ( 2 ) population was constructed from the cross between the msp1-4 ( japonica ) and LongTeFu B ( indica ) .
This locus was mapped between the two InDel markers , WY-4 and WY-8 , with physical distance of 247 kb .
A deletion with 10 base pairs between 758 bp and 767 bp in MSP1 open reading frame was confirmed by sequence analysis , which led to pre-termination of MSP1 translation .
Phenotype analysis of msp1-4 indicated that it was similar to the msp1 mutant .
To insight the expression change of rice anther developmental genes in this mutant , semi-quantitative RT-PCR analysis was carried out .
The results showed that the expression level of rice UDT1 and GAMYB were reduced in msp1-4 , implying that UDT1 and GAMYB are possibly the downstream genes of MSP1 gene in rice pollen development . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: [ Identification of the rice ( Oryza sativa L ) mutant msp1-4 and expression analysis of its UDT1 and GAMYB genes ]
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
