| 22 matches found in 9 documents. Search time: 0.257 seconds. |
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Score: 12.00 |
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| Title: Rice Triosephosphate Isomerase Gene 5 [ prime ] Sequence Directs [ beta ] -Glucuronidase Activity in Transgenic Tobacco but Requires an Intron for Expression in Rice .
| | Author: Xu Y Yu H Hall TC .
| | Journal: Citation: V : 106 ( 2 ) P : 459-467 Year: 1994 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12232342 Accession (PMID): 12232342 | | Abstract: In rice ( Oryza sativa L ) , cytosolic triosephosphate isomerase ( TPI ) is encoded by a single gene .
TPI catalyzes a vital step in glycolysis , and RNA blots showed that the tpi gene is expressed in all vegetative it issues ( root , culm , and leaves ) and in rice suspension cells .
No effect of light on expression was detected , but submergence of rice seedlings resulted in elevated levels of TPI mRNA in roots and culms .
The 2767-bp 5 [ prime ] upstream sequence of the tpi gene was fused translationally with the [ beta ] -glucuronidase ( gusA ) gene , and the resulting construct , TPI-GUS , was found to express constitutive , high levels of GUS activity in transgenic tobacco ( Nicotiana tabacum ) plants .
However , the same construct yielded no GUS activity in stably transformed rice plants , and RNA blots showed that no GUS mRNA could be detected even though stable integration of functional copies of the construct was confirmed by Southern blot and genomic polymerase chain reaction analyses .
Transient assays using particle bombardment yielded high levels of GUS expression from the TPI-GUS construct in tobacco leaves , but essentially no expression in rice , barley , or maize leaves .
When the first intron of the tpi gene was included in the construct ( TPI-int1-GUS ) , transient GUS activity was routinely obtained in rice leaves , revealing that the first intron of the rice tpi gene is crucial for its expression in rice .
TPI-int1-GUS also directed transient GUS expression in maize and barley leaves , but little or no activity was obtained from this construct in tobacco , tomato , or soybean leaves .
These results with the rice tpi promoter are in accordance with mounting evidence that differences in gene expression exist between monocots and dicots . | Matching Sentences:
[ Sen. 7, subscore: 3.00 ]: When the first intron of the tpi gene was included in the construct ( TPI-int1-GUS ) , transient GUS activity was routinely obtained in rice leaves , revealing that the first intron of the rice tpi gene is crucial for its expression in rice .
[ Sen. 2, subscore: 2.00 ]: TPI catalyzes a vital step in glycolysis , and RNA blots showed that the tpi gene is expressed in all vegetative it issues ( root , culm , and leaves ) and in rice suspension cells .
[ Sen. 4, subscore: 2.00 ]: The 2767-bp 5 [ prime ] upstream sequence of the tpi gene was fused translationally with the [ beta ] -glucuronidase ( gusA ) gene , and the resulting construct , TPI-GUS , was found to express constitutive , high levels of GUS activity in transgenic tobacco ( Nicotiana tabacum ) plants .
[ Sen. 1, subscore: 1.00 ]: In rice ( Oryza sativa L ) , cytosolic triosephosphate isomerase ( TPI ) is encoded by a single gene .
[ Sen. 3, subscore: 1.00 ]: No effect of light on expression was detected , but submergence of rice seedlings resulted in elevated levels of TPI mRNA in roots and culms .
[ Sen. 6, subscore: 1.00 ]: Transient assays using particle bombardment yielded high levels of GUS expression from the TPI-GUS construct in tobacco leaves , but essentially no expression in rice , barley , or maize leaves .
[ Sen. 8, subscore: 1.00 ]: TPI-int1-GUS also directed transient GUS expression in maize and barley leaves , but little or no activity was obtained from this construct in tobacco , tomato , or soybean leaves .
[ Sen. 9, subscore: 1.00 ]: These results with the rice tpi promoter are in accordance with mounting evidence that differences in gene expression exist between monocots and dicots .
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Score: 7.00 |
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| Title: Cytosolic triosephosphate isomerase is a single gene in rice .
| | Author: Xu Y Hall TC .
| | Journal: Plant Physiol .
Citation: V : 101 ( 2 ) P : 683-7 Year: 1993 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8278510 Accession (PMID): 8278510 | | Abstract: A cDNA clone encoding rice ( Oryza sativa L ) cytosolic triosephosphate isomerase ( TPI ) , an important glycolytic enzyme , was isolated and characterized .
The clone ( pRTPI-6 ) contains an open reading frame of 759 base pairs , encoding a polypeptide chain of 253 amino acid residues ( M ( r ) 27 , 060 ) .
The identity of this clone was defined by its high homology ( 85% nucleotide sequence and 89% amino acid sequence identical match ) with a maize mRNA sequence encoding the cytosolic TPI and with TPIs from other species .
Genomic DNA blot analysis using the cDNA as a probe showed that the cytosolic TPI gene is present as a single copy per haploid rice genome , as opposed to that found in maize , in which multiple TPI gene copies exist A single TPI mRNA species of about 1100 nucleotides was detected by gel blot hybridization analysis of RNA isolated from root , culm , and leaf it issues , indicating that its expression is ubiquitous .
Based on sequence comparison and molecular analysis , we propose that the chloroplast-located TPI may be encoded by divergent structural nuclear genes in rice . | Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: Genomic DNA blot analysis using the cDNA as a probe showed that the cytosolic TPI gene is present as a single copy per haploid rice genome , as opposed to that found in maize , in which multiple TPI gene copies exist A single TPI mRNA species of about 1100 nucleotides was detected by gel blot hybridization analysis of RNA isolated from root , culm , and leaf it issues , indicating that its expression is ubiquitous .
[ Sen. 3, subscore: 2.00 ]: The identity of this clone was defined by its high homology ( 85% nucleotide sequence and 89% amino acid sequence identical match ) with a maize mRNA sequence encoding the cytosolic TPI and with TPIs from other species .
[ Sen. 1, subscore: 1.00 ]: A cDNA clone encoding rice ( Oryza sativa L ) cytosolic triosephosphate isomerase ( TPI ) , an important glycolytic enzyme , was isolated and characterized .
[ Sen. 5, subscore: 1.00 ]: Based on sequence comparison and molecular analysis , we propose that the chloroplast-located TPI may be encoded by divergent structural nuclear genes in rice .
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Score: 5.00 |
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| Title: Intron position affects expression from the tpi promoter in rice .
| | Author: Snowden KC Buchhholz WG Hall TC .
| | Journal: Plant Mol . Biol . Citation: V : 31 ( 3 ) P : 689-92 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8790301 Accession (PMID): 8790301 | | Abstract: A series of promoter-GUS fusion constructs containing a portion of the rice triosephosphate isomerase ( tpi ) promoter , the first tpi intron , and the gene encoding bacterial beta-glucuronidase ( GUS ) were made .
These constructs were electroporated into rice protoplasts and transient expression was monitored .
Inclusion of the first intron from the rice tpi gene enhanced expression of the GUS gene from the tpi promoter when it was placed 5 of the GUS gene .
When the tpi intron was placed in the 3-untranslated region no enhancement of GUS gene expression was observed , indicating the importance of position in intron-mediated enhancement of gene expression . | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: A series of promoter-GUS fusion constructs containing a portion of the rice triosephosphate isomerase ( tpi ) promoter , the first tpi intron , and the gene encoding bacterial beta-glucuronidase ( GUS ) were made .
[ Sen. 3, subscore: 2.00 ]: Inclusion of the first intron from the rice tpi gene enhanced expression of the GUS gene from the tpi promoter when it was placed 5 of the GUS gene .
[ Sen. 4, subscore: 1.00 ]: When the tpi intron was placed in the 3-untranslated region no enhancement of GUS gene expression was observed , indicating the importance of position in intron-mediated enhancement of gene expression .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Changes in the contents of metabolites and enzyme activities in rice plants responding to Rhizoctonia solani Kuhn infection : activation of glycolysis and connection to phenylpropanoid pathway .
| | Author: Mutuku JM Nose A | | Journal: Plant Cell Physiol Citation: V : 53 P : 1017-32 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22492233 Accession (PMID): 22492233 | | Abstract: Rhizoctonia solani Kuhn causes sheath blight disease in rice , and genetic resistance against it is the most desirable characteristic .
Current improvement efforts are based on analysis of polygenic quantitative trait loci ( QTLs ) , but interpretation is limited by the lack of information on the changes in metabolic pathways .
Our previous studies linked activation of the glycolytic pathway to enhanced generation of lignin in the phenylpropanoid pathway .
The current studies investigated the regulation of glycolysis by examining the time course of changes in enzymatic activities and metabolite contents .
The results showed that the activities of all glycolytic enzymes as well as fructose-6-phosphate ( F-6-P ) , fructose-1 , 6-bisphosphate ( F-1 , 6-P ( 2 ) ) , dihydroxyacetone phosphate ( DHAP ) , glyceraldehyde-3-phosphate ( GAP ) , 3-phosphoglycerate ( 3-PG ) , phosphoenolpyruvate ( PEP ) and pyruvate contents increased .
These results combined with our previous findings that the expression of phosphoglucomutase ( PGM ) , triosephosphate isomerase ( TPI ) , glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) , enolase and pyruvate kinase ( PK ) increased after infection suggested that the additional establishment of glycolysis in the cytosol compartment occurred after infection .
Further evidence for this was our recent findings that the increase in expression of the 6-phosphofructokinase ( PFK ) plastid isozyme Os06g05860 was accompanied by an increase in expression of three cytosolic PFK isozymes , ie Os01g09570 , Os01g53680 and Os04g39420 , as well as pyrophosphate-dependent phosphofrucokinase ( PFP ) isozymes Os08g25720 ( alpha-subunit ) and Os06g13810 ( beta-subunit ) in infected rice plants of the resistant line .
The results also showed that the reactions catalysed by PFK/PFP , aldolase , GAPDH + phosphoglycerate kinase ( PGK ) and PK in leaf sheaths of R solani-infected rice plants were non-equilibrium reactions in vivo .
This study showed that PGM , phosphoglucose isomerase ( PGI ) , TPI and phosphoglycerate mutase ( PGmu ) + enolase could be regulated through coarse control whereas , PFK/PFP , aldolase , GAPDH + PGK and PK could be regulated through coarse and fine controls simultaneously .
| Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: These results combined with our previous findings that the expression of phosphoglucomutase ( PGM ) , triosephosphate isomerase ( TPI ) , glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) , enolase and pyruvate kinase ( PK ) increased after infection suggested that the additional establishment of glycolysis in the cytosol compartment occurred after infection .
[ Sen. 9, subscore: 1.00 ]: This study showed that PGM , phosphoglucose isomerase ( PGI ) , TPI and phosphoglycerate mutase ( PGmu ) + enolase could be regulated through coarse control whereas , PFK/PFP , aldolase , GAPDH + PGK and PK could be regulated through coarse and fine controls simultaneously .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: [ Phosphorus fractions under different land uses in Sanjiang plain ] | | Author: Qin SJ Liu JS Wang GP Zhou WM | | Journal: Huan Jing Ke Xue Citation: V : 28 P : 2777-82 Year: 2007 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18290436 Accession (PMID): 18290436 | | Abstract: Five land-uses type ( soybean-filed , rice-field , abandoned cultivation , artificial forest , natural wetland ) were obtained before planting and soil P fractions were determined by a modified Hedley fraction method .
The result showed that the content of total P ( TP ) in natural wetland was higher than other soil .
While the proportion of total inorganic P ( TPi ) in TP of farm land soil was higher than that of wetland .
Adversely , the proportion of total organic P ( TPo ) was higher in wetland and least in rice-field .
There was no obvious difference between that of abandoned field and artificial forest The values of labile inorganic phosphorus ( labile Pi ) under different land-uses were similar .
The contents of Resin-P and NaHCO3-Pi varied at a range of 32-36 . 3 mg x kg ( -1 ) and 33 . 77-50 . 42 mg x kg ( -1 ) , respectively .
The contents of NaOH-Pi and CHCl-Pi in farmland were higher than those of wetland , especially in rice-field which was 152 . 4 mg x kg ( -1 ) .
But for DHCl-Pi , the content was higher in wetland with the value of 84 . 3 mg x kg ( -1 ) , and the contents of NaOH-Pi , C HCl-Pi and DHCl-Pi in abandoned field and artificial forest were in the middle of the above two land use patterns , which indicated that the restoration of soil P in abandoned farmland tended to approach the level of natural wetland .
The value and percentage of Po was decreased greatly with the reclamation of wetland , and the decrease of C HCl-Po was the most significant in all forms of organic P , and its values in soybean-filed and rice-field were only 25 . 96% and 19 . 26% of that in wetland .
As the time went by the content of Po in abandoned arable land increased with very slow speed , which indicated that the restoration of wetland after reclamation would need a long time .
The distribution of soil P in different P fractions has significant difference for the land-use change in Sanjiang plain .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: While the proportion of total inorganic P ( TPi ) in TP of farm land soil was higher than that of wetland .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
