Score: 4.00 |
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| Title: Action of aluminum , novel TPC1-type channel inhibitor , against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells .
| | Author: Lin C Yu Y Kadono T Iwata M Umemura K Furuichi T Kuse M Isobe M Yamamoto Y Matsumoto H Yoshizuka K Kawano T | | Journal: Biochem . Biophys . Res . Commun . Citation: V : 332 ( 3 ) P : 823-30 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15913561 Accession (PMID): 15913561 | | Abstract: Previously , effect of Al ions on calcium signaling was assessed in tobacco cells expressing a Ca2+-monitoring luminescent protein , aequorin and a newly isolated putative plant Ca2+ channel protein from Arabidopsis thaliana , AtTPC1 ( two-pore channel 1 ) .
TPC1 channels were shown to be the only channel known to be sensitive to Al and they are responsive to reactive oxygen species and cryptogein , a fungal elicitor protein .
Thus , involvement of TPC1 channels in calcium signaling leading to development of plant defense mechanism has been suggested .
Then , the use of Al as a specific inhibitor of TPC1-type plant calcium channels has been proposed .
Here , using transgenic tobacco BY-2 cells expressing aequorin , we report on the evidence in support of the involvement of Al-sensitive signaling pathway requiring TPC1-type channel-dependent Ca2+ influx in response to salicylic acid , a key plant defense-inducing agent , but not to an elicitor prepared from the cell wall of rice blast disease fungus Magnaporthe grisea .
In addition , involvement of Al-sensitive Ca2+ channels in response to cold shock was also tested .
The data suggested that the elicitor used here induces the Ca2+ influx via Al-insensitive path , while salicylic acid and cold-shock-stimulate the influx of Ca2+ via Al-sensitive mechanism .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: TPC1 channels were shown to be the only channel known to be sensitive to Al and they are responsive to reactive oxygen species and cryptogein , a fungal elicitor protein .
[ Sen. 3, subscore: 1.00 ]: Thus , involvement of TPC1 channels in calcium signaling leading to development of plant defense mechanism has been suggested .
[ Sen. 4, subscore: 1.00 ]: Then , the use of Al as a specific inhibitor of TPC1-type plant calcium channels has been proposed .
[ Sen. 5, subscore: 1.00 ]: Here , using transgenic tobacco BY-2 cells expressing aequorin , we report on the evidence in support of the involvement of Al-sensitive signaling pathway requiring TPC1-type channel-dependent Ca2+ influx in response to salicylic acid , a key plant defense-inducing agent , but not to an elicitor prepared from the cell wall of rice blast disease fungus Magnaporthe grisea .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Action of aluminum , novel TPC1-type channel inhibitor , against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells .
| | Author: Lin C Yu Y Kadono T Iwata M Umemura K Furuichi T Kuse M Isobe M Yamamoto Y Matsumoto H Yoshizuka K Kawano T | | Journal: Biochem . Biophys . Res . Commun . Citation: V : 332 ( 3 ) P : 823-30 Year: 2005 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub15913561 Accession (PMID): 15913561 | | Abstract: Previously , effect of Al ions on calcium signaling was assessed in tobacco cells expressing a Ca2+-monitoring luminescent protein , aequorin and a newly isolated putative plant Ca2+ channel protein from Arabidopsis thaliana , AtTPC1 ( two-pore channel 1 ) .
TPC1 channels were shown to be the only channel known to be sensitive to Al and they are responsive to reactive oxygen species and cryptogein , a fungal elicitor protein .
Thus , involvement of TPC1 channels in calcium signaling leading to development of plant defense mechanism has been suggested .
Then , the use of Al as a specific inhibitor of TPC1-type plant calcium channels has been proposed .
Here , using transgenic tobacco BY-2 cells expressing aequorin , we report on the evidence in support of the involvement of Al-sensitive signaling pathway requiring TPC1-type channel-dependent Ca2+ influx in response to salicylic acid , a key plant defense-inducing agent , but not to an elicitor prepared from the cell wall of rice blast disease fungus Magnaporthe grisea .
In addition , involvement of Al-sensitive Ca2+ channels in response to cold shock was also tested .
The data suggested that the elicitor used here induces the Ca2+ influx via Al-insensitive path , while salicylic acid and cold-shock-stimulate the influx of Ca2+ via Al-sensitive mechanism .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Action of aluminum , novel TPC1-type channel inhibitor , against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
