| 13 matches found in 6 documents. Search time: 0.113 seconds. |
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| Title: Fine mapping of the rice thermo-sensitive genic male-sterile gene tms5 .
| | Author: Wang YG Xing QH Deng QY Liang FS Yuan LP Weng ML Wang B | | Journal: Theor . Appl . Genet . Citation: V : 107 ( 5 ) P : 917-21 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12827251 Accession (PMID): 12827251 | | Abstract: AnnongS-1 , a thermo-sensitive genic male-sterile ( TGMS ) rice line , has a new TGMS gene .
Genetic analysis indicated that the sterility of AnnongS-1 was controlled by a single resessive gene named tms5 .
In our previous studies based on an F ( 2 ) population from the cross between AnnongS-1 and Nanjing11 , tms5 was mapped on chromosome 2 .
Recently , a RIL ( recombinant inbred line ) population from the same cross was developed and used for the fine mapping of the tms5 gene .
Molecular marker techniques combined with BSA ( bulked segregant analysis ) were used .
As a result , two AFLP markers ( AF10 , AF8 ) , one RAPD marker ( RA4 ) , one STS marker ( C365-1 ) , one CAPs marker ( G227-1 ) and four SSR markers ( RM279 , RM492 , RM327 , RM324 ) were found to be closely linked to tms5 gene .
The DNA sequences of the RFLP marker of C365 and G227 were found in GenBank , and on the basis of these sequences , many primers were designed to amplify the two parents and their RIL population plants .
Finally , the tms5 gene was mapped between STS marker C365-1 and CAPs marker G227-1 at a distance of 1 . 04 cM from C365-1 and 2 . 08 cM from G227-1 . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Genetic analysis indicated that the sterility of AnnongS-1 was controlled by a single resessive gene named tms5 .
[ Sen. 3, subscore: 1.00 ]: In our previous studies based on an F ( 2 ) population from the cross between AnnongS-1 and Nanjing11 , tms5 was mapped on chromosome 2 .
[ Sen. 4, subscore: 1.00 ]: Recently , a RIL ( recombinant inbred line ) population from the same cross was developed and used for the fine mapping of the tms5 gene .
[ Sen. 6, subscore: 1.00 ]: As a result , two AFLP markers ( AF10 , AF8 ) , one RAPD marker ( RA4 ) , one STS marker ( C365-1 ) , one CAPs marker ( G227-1 ) and four SSR markers ( RM279 , RM492 , RM327 , RM324 ) were found to be closely linked to tms5 gene .
[ Sen. 8, subscore: 1.00 ]: Finally , the tms5 gene was mapped between STS marker C365-1 and CAPs marker G227-1 at a distance of 1 . 04 cM from C365-1 and 2 . 08 cM from G227-1 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
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| Title: Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping .
| | Author: Yang Q Liang C Zhuang W Li J Deng H Deng Q Wang B | | Journal: Planta Citation: V : 225 ( 2 ) P : 321-30 Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16896793 Accession (PMID): 16896793 | | Abstract: Previous research has demonstrated that the thermo-sensitive genic male-sterile ( TGMS ) gene in rice was regulated by temperature .
TGMS rice is important to hybrid rice production because the application of the TGMS system in two-line breeding is cost-effective , simple , efficient and overcomes the limitations of the cytoplasmic male sterility ( CMS ) system .
AnnongS is the first discovered and deeply studied TGMS rice line in China .
Previous studies have suggested that AnnongS-1 and Y58S , two derivative TGMS lines of AnnongS , were both controlled by a single recessive gene named tms5 , which was genetically mapped on chromosome 2 .
In the current study , three populations ( AnnongS-1 x Nanjing11 , Y58S x Q611 , and Y58S x Guanghui122 ) were developed to investigate the tms5 gene molecular map .
Analysis of recombination events of sterile samples , utilizing 125 probes covering the tms5 region , suggested that the tms5 gene was physically mapped to a 19 kb DNA fragment between two markers , 4039-1 and 4039-2 , located on the BAC clone AP004039 .
Following the construction of a physical map between the two markers , ONAC023 , a member of the NAC ( NAM-ATAF-CUC-related ) gene family , was identified as the candidate of the tms5 gene .
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[ Sen. 6, subscore: 2.00 ]: Analysis of recombination events of sterile samples , utilizing 125 probes covering the tms5 region , suggested that the tms5 gene was physically mapped to a 19 kb DNA fragment between two markers , 4039-1 and 4039-2 , located on the BAC clone AP004039 .
[ Sen. 4, subscore: 1.00 ]: Previous studies have suggested that AnnongS-1 and Y58S , two derivative TGMS lines of AnnongS , were both controlled by a single recessive gene named tms5 , which was genetically mapped on chromosome 2 .
[ Sen. 5, subscore: 1.00 ]: In the current study , three populations ( AnnongS-1 x Nanjing11 , Y58S x Q611 , and Y58S x Guanghui122 ) were developed to investigate the tms5 gene molecular map .
[ Sen. 7, subscore: 1.00 ]: Following the construction of a physical map between the two markers , ONAC023 , a member of the NAC ( NAM-ATAF-CUC-related ) gene family , was identified as the candidate of the tms5 gene .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Fine mapping of a gene for non-pollen type thermosensitive genic male sterility in rice ( Oryza sativa L ) .
| | Author: Peng HF Chen XH Lu YP Peng YF Wan BH Chen ND Wu B Xin SP Zhang GQ | | Journal: Theor Appl Genet Citation: V : 120 P : 1013-20 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20012261 Accession (PMID): 20012261 | | Abstract: The thermo-sensitive genic male sterility ( TGMS ) lines play a crucial role in two-line hybrid rice production .
For a practical TGMS line , the stability of male sterility is one of the most important technical indicators .
In this study , XianS , a spontaneous mutant with stable male sterility from an indica rice cultivar Xianhuangzhan , was classified as a non-pollen type TGMS line .
The critical non-pollen sterility point temperature of XianS was determined as 27 degrees C Genetic analysis demonstrated that the non-pollen sterility in XianS was controlled by a single recessive gene .
Using SSR markers and bulked segregant analysis , the TGMS gene in XianS was fine mapped to a 183 kb interval between RMAN81 and RMX21 on chromosome 2 .
Two markers , 4039-1 and RMX14 completely cosegregated with this gene .
Allelism test indicated that the non-pollen phenotype in seven non-pollen type TGMS lines from different sources , XianS , AnnongS-1 , Q523S , Q524S , N28S , G421S , and Q527S is caused by the same TGMS gene .
Although the location of TGMS gene in XianS is close to the gene OsNAC6 , a previously identified candidate gene of tms5 in AnnongS-1 , the sequence of OsNAC6 and its promoter region was identical in TGMS line XianS , AnnongS-1 , and wild-type Xianhuangzhan .
These results suggest that the non-pollen type TGMS trait probably be controlled by the same TGMS gene in different TGMS rice lines , but its real candidate gene still need to be further studied and identified .
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[ Sen. 8, subscore: 1.00 ]: Although the location of TGMS gene in XianS is close to the gene OsNAC6 , a previously identified candidate gene of tms5 in AnnongS-1 , the sequence of OsNAC6 and its promoter region was identical in TGMS line XianS , AnnongS-1 , and wild-type Xianhuangzhan .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Identification of amino acids important for substrate specificity in sucrose transporters using gene shuffling .
| | Author: Reinders A Sun Y Karvonen KL Ward JM | | Journal: J Biol Chem Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22807445 Accession (PMID): 22807445 | | Abstract: Plant sucrose transporters ( SUTs ) are H+-coupled uptake transporters .
Type I and II ( SUTs ) are phylogenetically related but have different substrate specificities .
Type I SUTs transport sucrose , maltose and a wide range of natural and synthetic alpha and beta-glucosides .
Type II SUTs are more selective for sucrose and maltose .
Here , we investigated the structural basis for this difference in substrate specificity .
We used a novel gene shuffling method called Synthetic Template Shuffling ( STS ) to introduce 62 differentially conserved amino acid residues from type I SUTs into OsSUT1 , a type II SUT from rice .
The OsSUT1 variants were tested for their ability to transport the fluorescent coumarin beta-glucoside esculin when expressed in yeast Fluorescent yeast cells were selected using fluorescence-activated cell sorting ( FACS ) .
Substitution of five amino acids present in type I SUTs in OsSUT1 was found to be sufficient to confer esculin uptake activity .
The changes clustered in two areas of the OsSUT1 protein , in the first loop and the top of TMS2 ( T80L , A86K ) and in TMS5 ( S220A , S221A , T224Y ) .
The substrate specificity of this OsSUT1 variant was almost identical to that of type I SUTs .
Corresponding changes in the sugarcane type II transporter ShSUT1 also changed substrate specificity indicating that these residues contribute to substrate specificity in type II SUTs in general | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: The changes clustered in two areas of the OsSUT1 protein , in the first loop and the top of TMS2 ( T80L , A86K ) and in TMS5 ( S220A , S221A , T224Y ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Fine mapping of the rice thermo-sensitive genic male-sterile gene tms5 .
| | Author: Wang YG Xing QH Deng QY Liang FS Yuan LP Weng ML Wang B | | Journal: Theor . Appl . Genet . Citation: V : 107 ( 5 ) P : 917-21 Year: 2003 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub12827251 Accession (PMID): 12827251 | | Abstract: AnnongS-1 , a thermo-sensitive genic male-sterile ( TGMS ) rice line , has a new TGMS gene .
Genetic analysis indicated that the sterility of AnnongS-1 was controlled by a single resessive gene named tms5 .
In our previous studies based on an F ( 2 ) population from the cross between AnnongS-1 and Nanjing11 , tms5 was mapped on chromosome 2 .
Recently , a RIL ( recombinant inbred line ) population from the same cross was developed and used for the fine mapping of the tms5 gene .
Molecular marker techniques combined with BSA ( bulked segregant analysis ) were used .
As a result , two AFLP markers ( AF10 , AF8 ) , one RAPD marker ( RA4 ) , one STS marker ( C365-1 ) , one CAPs marker ( G227-1 ) and four SSR markers ( RM279 , RM492 , RM327 , RM324 ) were found to be closely linked to tms5 gene .
The DNA sequences of the RFLP marker of C365 and G227 were found in GenBank , and on the basis of these sequences , many primers were designed to amplify the two parents and their RIL population plants .
Finally , the tms5 gene was mapped between STS marker C365-1 and CAPs marker G227-1 at a distance of 1 . 04 cM from C365-1 and 2 . 08 cM from G227-1 . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Fine mapping of the rice thermo-sensitive genic male-sterile gene tms5 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
