Score: 6.00 |
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| Title: A bacterial-type ABC transporter is involved in aluminum tolerance in rice .
| | Author: Huang CF Yamaji N Mitani N Yano M Nagamura Y Ma JF | | Journal: Plant Cell Citation: V : 21 P : 655-67 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19244140 Accession (PMID): 19244140 | | Abstract: Aluminum ( Al ) toxicity is a major factor limiting crop production in acidic soil , but the molecular mechanisms of Al tolerance are poorly understood .
Here , we report that two genes , STAR1 ( for sensitive to Al rhizotoxicity1 ) and STAR2 , are responsible for Al tolerance in rice .
STAR1 encodes a nucleotide binding domain , while STAR2 encodes a transmembrane domain , of a bacterial-type ATP binding cassette ( ABC ) transporter .
Disruption of either gene resulted in hypersensitivity to aluminum toxicity .
Both STAR1 and STAR2 are expressed mainly in the roots and are specifically induced by Al exposure .
Expression in onion epidermal cells , rice protoplasts , and yeast showed that STAR1 interacts with STAR2 to form a complex that localizes to the vesicle membranes of all root cells , except for those in the epidermal layer of the mature zone .
When expressed together in Xenopus laevis oocytes , STAR1/2 shows efflux transport activity specific for UDP-glucose .
Furthermore , addition of exogenous UDP-glucose rescued root growth in the star1 mutant exposed to Al These results indicate that STAR1 and STAR2 form a complex that functions as an ABC transporter , which is required for detoxification of Al in rice .
The ABC transporter transports UDP-glucose , which may be used to modify the cell wall .
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[ Sen. 8, subscore: 2.00 ]: Furthermore , addition of exogenous UDP-glucose rescued root growth in the star1 mutant exposed to Al These results indicate that STAR1 and STAR2 form a complex that functions as an ABC transporter , which is required for detoxification of Al in rice .
[ Sen. 2, subscore: 1.00 ]: Here , we report that two genes , STAR1 ( for sensitive to Al rhizotoxicity1 ) and STAR2 , are responsible for Al tolerance in rice .
[ Sen. 3, subscore: 1.00 ]: STAR1 encodes a nucleotide binding domain , while STAR2 encodes a transmembrane domain , of a bacterial-type ATP binding cassette ( ABC ) transporter .
[ Sen. 5, subscore: 1.00 ]: Both STAR1 and STAR2 are expressed mainly in the roots and are specifically induced by Al exposure .
[ Sen. 6, subscore: 1.00 ]: Expression in onion epidermal cells , rice protoplasts , and yeast showed that STAR1 interacts with STAR2 to form a complex that localizes to the vesicle membranes of all root cells , except for those in the epidermal layer of the mature zone .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice .
| | Author: Yamaji N Huang CF Nagao S Yano M Sato Y Nagamura Y Ma JF | | Journal: Plant Cell Citation: V : 21 P : 3339-49 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19880795 Accession (PMID): 19880795 | | Abstract: Aluminum ( Al ) toxicity is the major limiting factor of crop production on acid soils , but some plant species have evolved ways of detoxifying Al Here , we report a C2H2-type zinc finger transcription factor ART1 ( for Al resistance transcription factor 1 ) , which specifically regulates the expression of genes related to Al tolerance in rice ( Oryza sativa ) .
ART1 is constitutively expressed in the root , and the expression level is not affected by Al treatment .
ART1 is localized in the nucleus of all root cells .
A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1 , an important factor in rice Al tolerance .
Microarray analysis revealed 31 downstream transcripts regulated by ART1 , including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants .
Some of these genes were implicated in both internal and external detoxification of Al at different cellular levels .
Our findings shed light on comprehensively understanding how plants detoxify aluminum to survive in an acidic environment .
| Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1 , an important factor in rice Al tolerance .
[ Sen. 5, subscore: 1.00 ]: Microarray analysis revealed 31 downstream transcripts regulated by ART1 , including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
