| 6 matches found in 4 documents. Search time: 0.075 seconds. |
|---|
|
Score: 3.00 |
|---|
| Title: The rice SPINDLY gene functions as a negative regulator of gibberellin signaling by controlling the suppressive function of the DELLA protein , SLR1 , and modulating brassinosteroid synthesis .
| | Author: Shimada A Ueguchi-Tanaka M Sakamoto T Fujioka S Takatsuto S Yoshida S Sazuka T Ashikari M Matsuoka M | | Journal: Plant J Citation: V : 48 ( 3 ) P : 390-402 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17052323 Accession (PMID): 17052323 | | Abstract: SPINDLY ( SPY ) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin ( GA ) signaling through an unknown mechanism .
To understand the function of SPY in GA signaling in rice , we isolated a rice SPINDLY homolog ( OsSPY ) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct .
In knockdown plants , the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression , similar to the spindly phenotype of Arabidopsis spy mutants , suggesting that OsSPY also functions as a negative factor in GA signaling in rice .
The suppressive function of OsSPY in GA signaling was supported by the findings that the dwarfism was partially rescued and OsGA20ox2 ( GA20 oxidase ) expression was reduced in GA-deficient and GA-insensitive mutants by the knockdown of OsSPY function .
The suppression of OsSPY function in a GA-insensitive mutant , gid2 , also caused an increase in the phosphorylation of a rice DELLA protein , SLR1 , but did not change the amount of SLR1 .
This indicates that the function of OsSPY in GA signaling is not via changes in the amount or stability of SLR1 , but probably involves control of the suppressive function of SLR1 .
In addition to the GA-related phenotypes , OsSPY antisense and RNAi plants showed increased lamina joint bending , which is a brassinosteroid-related phenotype , indicating that OsSPY may play roles both in GA signaling and in the brassinosteroid pathway . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: SPINDLY ( SPY ) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin ( GA ) signaling through an unknown mechanism .
[ Sen. 2, subscore: 1.00 ]: To understand the function of SPY in GA signaling in rice , we isolated a rice SPINDLY homolog ( OsSPY ) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct .
[ Sen. 3, subscore: 1.00 ]: In knockdown plants , the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression , similar to the spindly phenotype of Arabidopsis spy mutants , suggesting that OsSPY also functions as a negative factor in GA signaling in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Gibbestatin B inhibits the GA-induced expression of alpha-amylase expression in cereal seeds .
| | Author: Hayashi K Inoguchi M Kondo H Nozaki H | | Journal: Phytochemistry Citation: V : 55 ( 1 ) P : 1-9 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11021637 Accession (PMID): 11021637 | | Abstract: The expression of alpha-amylase in aleurone layers of barley is known to be induced by gibberellin A3 ( GA ) .
In the present study , gibbestatin B ( GNB ) was isolated from Streptomyces sp .
C-39 as an inhibitor of the GA-induced expression of alpha-amylase in barley and rice , with IC50 values of 125 and 70 microM , respectively .
GNB suppressed accumulation of GA-induced barley high-pI type B and rice RAmylA alpha-amylase transcripts .
However , GNB showed no inhibitory activity on GUS expression in transgenic tobacco harboring the auxin-inducible par B promoter : : GUS fusion gene .
The transcription of an abscisic acid ( ABA ) -inducible gene , HVA1 , was unaffected by GNB .
In addition , GNB prevented aleurone cells from cell death induced by GA .
In tobacco and Arabidopsis plants , GNB suppressed the germination and retarded the growth of seedlings without toxicity .
The growth of gai , spy and abi mutants was also retarded by GNB .
Normal plants treated with GA-biosynthesis inhibitors and GA-defective and GA-signaling mutants normally have dwarf dark green leaves .
However , dwarfed healthy green leaves were observed in normal plants treated with GNB .
GA-induced stem elongation of plants was also detected in the presence of GNB .
These analyses indicate that GNB inhibits the GA-induced expression of alpha-amylase by regulating one of the steps involved in ABA signaling , but not by acting as a weak ABA analog . | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: The growth of gai , spy and abi mutants was also retarded by GNB .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: cDNA-AFLP analysis of inducible gene expression in rice seminal root tips under a water deficit .
| | Author: Yang L Zheng B Mao C Yi K Liu F Wu Y Tao Q Wu P | | Journal: Gene Citation: V : 314 ( ) P : 141-8 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14527726 Accession (PMID): 14527726 | | Abstract: The seminal roots of an upland rice variety , Azucena , showed accelerated elongation in response to a water deficit .
The elongation of cortical cells in the elongation zone is rapidly stimulated within 16 h by the water deficit . cDNA-AFLP analysis was used to examine gene expression in seminal root tips at four time points ( 4 , 16 , 48 and 72 h ) during the water deficit .
One hundred and six unique genes induced by the water deficit were obtained .
The expression patterns of these genes were confirmed by Northern blot analysis based on 21 selected genes representing different patterns .
The 106 upregulated genes were composed of 60 genes of known function , 28 genes of unknown function and 18 novel genes .
Sixty genes of known functions were involved in transport facilitation , metabolism and energy , stress and defense-related proteins , cellular organization and cell-wall biogenesis , signal transduction , expression regulator and transposable element , suggesting that seminal root tips undergo a complex adaptive process in response to the water deficit .
Expression of 22 genes reached a maximum within 16 h of water deficit treatment .
These included aquaporin ( PIP2a ) , 9-cis-epoxycarotenoid dioxygenase ( NCED1 ) and a negative regulator of gibberellin signal transduction ( SPY ) ; eight other genes participated in cell wall loosening or vesicle traffic . | Matching Sentences:
[ Sen. 8, subscore: 1.00 ]: These included aquaporin ( PIP2a ) , 9-cis-epoxycarotenoid dioxygenase ( NCED1 ) and a negative regulator of gibberellin signal transduction ( SPY ) ; eight other genes participated in cell wall loosening or vesicle traffic .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Expression of OsSPY and 14-3-3 genes involved in plant height variations of ion-beam-induced KDML 105 rice mutants .
| | Author: Phanchaisri B Samsang N Yu L Singkarat S Anuntalabhochai S | | Journal: Mutat Res Citation: V : 734 P : 56-61 Year: 2012 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub22445891 Accession (PMID): 22445891 | | Abstract: The culm length of two semidwarf rice mutants ( PKOS1 , HyKOS1 ) obtained from low-energy N-ion beam bombardments of dehusked Thai jasmine rice ( Oryza sativa L cv .
KDML 105 ) seeds showed 25 . 7% and 21 . 5% height reductions and one spindly rice mutant ( TKOS4 ) showed 21 . 4% increase in comparison with that of the KDML 105 control .
A cDNA-RAPD analysis identified differential gene expression in internode it issues of the rice mutants .
Two genes identified from the cDNA-RAPD were OsSPY and 14-3-3 , possibly associated with stem height variations of the semidwarf and spindly mutants , respectively .
The OsSPY gene encoded the SPY protein which is considered to be a negative regulator of gibberellin ( GA ) .
On the other hand , the 14-3-3 encoded a signaling protein which can bind and prevent the RSG ( repression of shoot growth ) protein function as a transcriptional repressor of the kaurene oxidase ( KO ) gene in the GA biosynthetic pathway .
Expression analysis of OsSPY , 14-3-3 , RSG , KO , and SLR1 was confirmed in rice internode it issues during the reproductive stage of the plants by semi-quantitative RT-PCR technique .
The expression analysis showed a clear increase of the levels of OsSPY transcripts in PKOS1 and HyKOS1 it issue samples compared to that of the KDML 105 and TKOS4 samples at the age of 50-60 days which were at the ages of internode elongation .
The 14-3-3 expression had the highest increase in the TKOS4 samples compared to those in KDML 105 , PKOS1 and HyKOS1 samples .
The expression analysis of RSG and KO showed an increase in TKOS4 samples compared to that of the KDML 105 and that of the two semidwarf mutants .
These results indicate that changes of OsSPY and 14-3-3 expression could affect internode elongation and cause the phenotypic changes of semidwarf and spindly rice mutants , respectively .
| Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: The OsSPY gene encoded the SPY protein which is considered to be a negative regulator of gibberellin ( GA ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
