| 11 matches found in 4 documents. Search time: 0.032 seconds. |
|---|
|
Score: 9.00 |
|---|
| Title: A rice spotted leaf gene , Spl7 , encodes a heat stress transcription factor protein .
| | Author: Yamanouchi U Yano M Lin H Ashikari M Yamada K | | Journal: Proc . Natl . Acad . Sci . USA Citation: V : 99 ( 11 ) P : 7530-5 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12032317 Accession (PMID): 12032317 | | Abstract: A rice spotted leaf ( lesion-mimic ) gene , Spl7 , was identified by map-based cloning .
High-resolution mapping with cleaved amplified polymorphic sequence markers enabled us to define a genomic region of 3 kb as a candidate for Spl7 .
We found one ORF that showed high similarity to a heat stress transcription factor ( HSF ) .
Transgenic analysis verified the function of the candidate gene for Spl7 : leaf spot development was suppressed in spl7 mutants with a wild-type Spl7 transgene .
Thus , we conclude that Spl7 encodes the HSF protein .
The transcript of spl7 was observed in mutant plants .
The levels of mRNAs ( Spl7 in wild type and spl7 in mutant ) increased under heat stress .
Sequence analysis revealed only one base substitution in the HSF DNA-binding domain of the mutant allele , causing a change from tryptophan to cysteine . | Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: Transgenic analysis verified the function of the candidate gene for Spl7 : leaf spot development was suppressed in spl7 mutants with a wild-type Spl7 transgene .
[ Sen. 7, subscore: 2.00 ]: The levels of mRNAs ( Spl7 in wild type and spl7 in mutant ) increased under heat stress .
[ Sen. 1, subscore: 1.00 ]: A rice spotted leaf ( lesion-mimic ) gene , Spl7 , was identified by map-based cloning .
[ Sen. 2, subscore: 1.00 ]: High-resolution mapping with cleaved amplified polymorphic sequence markers enabled us to define a genomic region of 3 kb as a candidate for Spl7 .
[ Sen. 5, subscore: 1.00 ]: Thus , we conclude that Spl7 encodes the HSF protein .
[ Sen. 6, subscore: 1.00 ]: The transcript of spl7 was observed in mutant plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: Regulatory mechanisms of ROI generation are affected by rice spl mutations .
| | Author: Kojo K Yaeno T Kusumi K Matsumura H Fujisawa S Terauchi R Iba K | | Journal: Plant Cell Physiol .
Citation: V : 47 ( 8 ) P : 1035-44 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16816407 Accession (PMID): 16816407 | | Abstract: Reactive oxygen intermediates ( ROIs ) play a pivotal role in the hypersensitive response ( HR ) in disease resistance .
NADPH oxidase is a major source of ROI ; however , the mechanisms of its regulation are unclear .
Rice spl mutants spontaneously form lesions which resemble those occurring during the HR , suggesting that the mutations affect regulation of the HR .
We found that spl2 , spl7 and spl11 mutant cells accumulated increased amounts of H ( 2 ) O ( 2 ) in response to rice blast fungal elicitor .
Increased accumulation of ROIs was suppressed by inhibition of NADPH oxidase in the spl cells , and was also observed in the ozone-exposed spl plants .
These mutants have sufficient activities of ROI-scavenging enzymes compared with the wild type .
In addition , spl7 mutant cells accumulated higher amounts of H ( 2 ) O ( 2 ) when treated with calyculin A ( CA ) , an inhibitor of protein phosphatase .
Furthermore , spl2 mutant plants exhibited accelerated accumulation of H ( 2 ) O ( 2 ) and increased rates of cell death in response to wounding .
These results suggest that the spl2 , spl7 and spl11 mutants are defective in the regulation of NADPH oxidase , and the spl7 mutation may give rise to enhancement of the signaling pathway which protein dephosphorylation controls , while the spl2 mutation affects both the pathogen-induced and wound-induced signaling pathways . | Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: These results suggest that the spl2 , spl7 and spl11 mutants are defective in the regulation of NADPH oxidase , and the spl7 mutation may give rise to enhancement of the signaling pathway which protein dephosphorylation controls , while the spl2 mutation affects both the pathogen-induced and wound-induced signaling pathways .
[ Sen. 4, subscore: 1.00 ]: We found that spl2 , spl7 and spl11 mutant cells accumulated increased amounts of H ( 2 ) O ( 2 ) in response to rice blast fungal elicitor .
[ Sen. 7, subscore: 1.00 ]: In addition , spl7 mutant cells accumulated higher amounts of H ( 2 ) O ( 2 ) when treated with calyculin A ( CA ) , an inhibitor of protein phosphatase .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Copper delivery by the copper chaperone for chloroplast and cytosolic copper/zinc-superoxide dismutases : regulation and unexpected phenotypes in an Arabidopsis mutant .
| | Author: Cohu CM Abdel-Ghany SE Gogolin Reynolds KA Onofrio AM Bodecker JR Kimbrel JA Niyogi KK Pilon M | | Journal: Mol Plant Citation: V : 2 P : 1336-50 Year: 2009 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub19969519 Accession (PMID): 19969519 | | Abstract: Copper ( Cu ) is an important mineral nutrient found in chloroplasts as a cofactor associated with plastocyanin and Cu/Zn superoxide dismutase ( Cu/ZnSOD ) .
Superoxide dismutases are metallo-enzymes found in most oxygenic organisms with proposed roles in reducing oxidative stress .
Several recent studies in Arabidopsis have shown that microRNAs and a SQUAMOSA promoter binding protein-like7 ( SPL7 ) transcription factor function to down-regulate the expression of many Cu-proteins , including Cu/ZnSOD in both plastids and the cytosol , during growth on low Cu .
Plants contain the Cu Chaperone for SOD ( CCS ) that delivers Cu to Cu/ZnSODs , and , in Arabidopsis , both cytosolic and plastidic CCS versions are encoded by one gene .
In this study , we demonstrate that Arabidopsis CCS transcript levels are regulated by Cu , mediated by microRNA 398 that was not previously predicted to target CCS .
The microRNA target site is conserved in CCS of Oryza sativa .
The data suggest that Cu-regulated microRNAs may have more mRNA targets than was previously predicted .
A CCS null mutant has no measurable SOD activity in the chloroplast and cytosol , indicating an absolute requirement for CCS .
When the CCS null mutant was grown on high Cu media , it lacked both Fe superoxide dismutase ( FeSOD ) and Cu/ZnSOD activity .
However , this did not lead to a visual phenotype and no photosynthetic deficiencies were detected , even after high light stress .
These results indicate that Cu/ZnSOD is not a pivotal component of the photosynthetic anti-oxidant system during growth in laboratory conditions .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Several recent studies in Arabidopsis have shown that microRNAs and a SQUAMOSA promoter binding protein-like7 ( SPL7 ) transcription factor function to down-regulate the expression of many Cu-proteins , including Cu/ZnSOD in both plastids and the cytosol , during growth on low Cu .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: A rice spotted leaf gene , Spl7 , encodes a heat stress transcription factor protein .
| | Author: Yamanouchi U Yano M Lin H Ashikari M Yamada K | | Journal: Proc . Natl . Acad . Sci . USA Citation: V : 99 ( 11 ) P : 7530-5 Year: 2002 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub12032317 Accession (PMID): 12032317 | | Abstract: A rice spotted leaf ( lesion-mimic ) gene , Spl7 , was identified by map-based cloning .
High-resolution mapping with cleaved amplified polymorphic sequence markers enabled us to define a genomic region of 3 kb as a candidate for Spl7 .
We found one ORF that showed high similarity to a heat stress transcription factor ( HSF ) .
Transgenic analysis verified the function of the candidate gene for Spl7 : leaf spot development was suppressed in spl7 mutants with a wild-type Spl7 transgene .
Thus , we conclude that Spl7 encodes the HSF protein .
The transcript of spl7 was observed in mutant plants .
The levels of mRNAs ( Spl7 in wild type and spl7 in mutant ) increased under heat stress .
Sequence analysis revealed only one base substitution in the HSF DNA-binding domain of the mutant allele , causing a change from tryptophan to cysteine . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A rice spotted leaf gene , Spl7 , encodes a heat stress transcription factor protein .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
