| 7 matches found in 4 documents. Search time: 0.166 seconds. |
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| Title: Proteome analysis and characterization of phenotypes of lesion mimic mutant spotted leaf 6 in rice .
| | Author: Kang SG Matin MN Bae H Natarajan S | | Journal: Proteomics Citation: V : 7 P : 2447-58 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17623303 Accession (PMID): 17623303 | | Abstract: Rice spotted leaf 6 ( spl6 ) mutant produces lesions caused by spontaneous cell death in the absence of pathogenic infection .
Expression of this genetic trait was developmentally programmed .
After the tillering stage , small red and brown lesions were initiated in groups on the leaf blade .
Eventually , the lesions formed parallel lines along the midrib of the leaf .
Under light and transmission electron microscopy , we observed that thylakoid membranes of mesophyll chloroplasts were progressively damaged in the nonspotted section of the mutant leaf .
However , chloroplasts were absent in the mesophyll cells of the spotted area of the spl6 mutant .
These results indicated that lesion formation of the spl6 mutant might be caused by oxidative burst Proteome analysis revealed that 159 protein spots were up or downregulated in comparison between spotted leaves of the spl6 mutant plants and normal leaves of the wild type .
Among them , protein disulfide isomerase ( PDI ) , transketolase , thioredoxin peroxidase ( TPX ) , ATP synthase , RuBisCO large subunit , and RuBisCO activase small subunit were not identified in the spl6 mutant but were abundant in the wild type .
Especially , the absence of TPX and PDI might be the cause of the failure to protect cells against oxidative burst resulting in degradation of the thylakoid membranes and leading to programmed cell death and lesion development .
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[ Sen. 7, subscore: 2.00 ]: These results indicated that lesion formation of the spl6 mutant might be caused by oxidative burst Proteome analysis revealed that 159 protein spots were up or downregulated in comparison between spotted leaves of the spl6 mutant plants and normal leaves of the wild type .
[ Sen. 1, subscore: 1.00 ]: Rice spotted leaf 6 ( spl6 ) mutant produces lesions caused by spontaneous cell death in the absence of pathogenic infection .
[ Sen. 6, subscore: 1.00 ]: However , chloroplasts were absent in the mesophyll cells of the spotted area of the spl6 mutant .
[ Sen. 8, subscore: 1.00 ]: Among them , protein disulfide isomerase ( PDI ) , transketolase , thioredoxin peroxidase ( TPX ) , ATP synthase , RuBisCO large subunit , and RuBisCO activase small subunit were not identified in the spl6 mutant but were abundant in the wild type .
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| Title: Inheritance of alleles for glutelin alpha-2 subunit genes in rice and identification of their corresponding cDNA clone .
| | Author: Qu Q Wei L Satoh H Kumamaru T Ogawa M Takaiwa F | | Journal: Citation: V : 105 ( 8 ) P : 1099-1108 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12582887 Accession (PMID): 12582887 | | Abstract: Rice glutelins consist of acidic ( alpha ) and basic ( beta ) subunits which are further separated into three polypeptide components assigned as alpha-1 , alpha-2 , and alpha-3 subunit components and beta-1 , beta-2 and beta-3 subunit components .
Nine rice mutant lines with a decreased amount of the glutelin alpha-2 subunit component ( alpha-2L ) were obtained by screening about 6 , 800 potential mutant lines derived from the fertilized egg treatment with N-methyl-N-nitrosourea ( MNU ) using the SDS-PAGE method .
The mutants were classified into three types of the increased alpha-1 subunit ( alpha-1H/alpha-2L ) , the decreased beta-2 subunit ( beta-2L/alpha-2L ) and the increased alpha-3 subunit ( alpha-3H/alpha-2L ) represented by EM278 , CM1707 and EM659 , respectively .
Iso-electric focus ( IEF ) analysis revealed that all of the mutants had an extremely low amount of a polypeptide with a 6 . 71 pI value , whereas a polypeptide with either a 6 . 50 pI value or with a 6 . 90 pI value increased significantly in alpha-1H/alpha-2L mutants or in alpha-3H/alpha-2L mutants , respectively .
The beta-2L/alpha-2L mutants had a decreased amount of a basic polypeptide with a 8 . 74 pI value .
Genetic analysis revealed that the three types of mutants were controlled by a single incomplete dominant gene respectively , and the three are alleles .
The gene was temporarily named glu4 , which was found to be located on chromosome 1 linked with the eg and spl6 genes .
Two-dimensional electrophoresis analysis revealed that the glu4 encoded polypeptides of pI 6 . 71/alpha-2 and pI 8 . 74/beta-2 .
Amino acid sequence analysis suggested that the mutated acidic polypeptide was the product of a GluA subfamily gene .
Northern and RT-PCR analyses revealed that glu4 corresponded to the GluA-1 gene .
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[ Sen. 7, subscore: 1.00 ]: The gene was temporarily named glu4 , which was found to be located on chromosome 1 linked with the eg and spl6 genes .
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| Title: Rice lesion mimic mutants with enhanced resistance to diseases .
| | Author: Wu C Bordeos A Madamba MR Baraoidan M Ramos M Wang GL Leach JE Leung H | | Journal: Mol Genet Genomics Citation: V : 279 P : 605-19 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18357468 Accession (PMID): 18357468 | | Abstract: Lesion mimic mutants are characterized by the formation of necrotic lesions in the absence of pathogens .
Such genetic defects often result in enhanced resistance to pathogen infection and constitutive expression of defense response genes .
To understand the genetic mechanisms leading to these mutations , we characterized 21 lesion mimic mutants isolated from IR64 rice mutant populations produced by mutagenesis with diepoxybutane ( D ) , gamma rays ( G ) , and fast neutrons ( F ) .
Four mutations are controlled by single dominant genes , one of which is inherited maternally .
Five lesion mimics are allelic to known spotted leaf ( spl ) mutants spl1 , spl2 , spl3 , or spl6 .
In total , 11 new lesion mimic mutations , named spl16 , spl17 , and spl19 through Spl27 , were established based on allelism tests .
Two lesion mimics , spl17 and Spl26 showed enhanced resistance to multiple strains of Magnaporthe oryzae , the rice blast pathogen , and Xanthomonas oryzae pv . oryzae , the bacterial blight ( BB ) pathogen .
Co-segregation analyses of blast and BB resistance and lesion mimic phenotypes in segregating populations of spl17 and Spl26 indicate that enhanced resistance to the two diseases is conferred by mutations in the lesion mimic genes .
A double mutant produced from two independent lesion mimics showed more severe lesions and higher level of resistance to X o . pv . oryzae than their single mutant parents indicating a synergistic effect of the two mutations .
In mutants that exhibit enhanced disease resistance to both pathogens , increases in expression of defense response genes PR-10a , POX22 . 3 , and PO-C1 were correlated with lesion mimic development and enhancement of resistance .
These lesion mimic mutants may provide essential materials for a comprehensive dissection of the disease resistance pathways in rice .
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[ Sen. 5, subscore: 1.00 ]: Five lesion mimics are allelic to known spotted leaf ( spl ) mutants spl1 , spl2 , spl3 , or spl6 .
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| Title: Comparative phenotypic and physiological characteristics of spotted leaf 6 ( spl6 ) and brown leaf spot2 ( bl2 ) lesion mimic mutants ( LMM ) in rice .
| | Author: Matin MN Saief SA Rahman MM Lee DH Kang H Lee DS Kang SG | | Journal: Mol Cells Citation: V : 30 P : 533-43 Year: 2010 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub21110131 Accession (PMID): 21110131 | | Abstract: Spontaneous necrotic lesions were found in a lesion mimic mutant brown leaf spot 2 ( bl2 ) without pathogenic infection .
Small spots in the seedlings appeared at the four leaves stage and gradually grew into a large round and black area with a gray center on the leaf surfaces .
Lower growth habit and lower agronomic trait values with reduced stature , tiller , and panicle number , as well as lower yield potential were noted in the mutants relative to the trait values of the wild-type plants .
Microscopic analysis revealed that mesophyll chloroplast was severely damaged or absent in the spotted area of the mutant leaves .
Total chlorophyll content , hydrogen peroxide level , and catalase activity were increased at up to 45 days after germination and were dropped at 60 d in the mutant leaves .
However , the total protein contents were reduced slightly with a growth period of up to 45 days and were increased at 60 days after germination .
A gradual increment of the total ascorbic acid contents in the mutants were observed with advanced plant age , but increased until 45 days and dropped comparatively at 60 days in the wild-type leaves .
Increased gene transcriptions of OsPDI and OsGPX1 were noted in the spotted leaves as compared to the non-spotted leaves of the mutant and wild-type leaves , whereas transcripts of OsTPX were transcribed at lower levels in the spotted leaves as compared to the non-spotted leaves .
The genetic nature of the bl2 mutant indicated that the F ( 1 ) plants evidenced the wild-type phenotype and that bl2 was governed by a single recessive gene .
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[ Sen. 1, subscore: 1.00 ]: Comparative phenotypic and physiological characteristics of spotted leaf 6 ( spl6 ) and brown leaf spot2 ( bl2 ) lesion mimic mutants ( LMM ) in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
