| 56 matches found in 17 documents. Search time: 0.301 seconds. |
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Score: 10.00 |
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| Title: Spotted leaf11 , a negative regulator of plant cell death and defense , encodes a U-box/armadillo repeat protein endowed with E3 ubiquitin ligase activity .
| | Author: Zeng LR Qu S Bordeos A Yang C Baraoidan M Yan H Xie Q Nahm BH Leung H Wang GL .
| | Journal: Plant Cell Citation: V : 16 ( 10 ) P : 2795-808 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15377756 Accession (PMID): 15377756 | | Abstract: The rice ( Oryza sativa ) spotted leaf11 ( spl11 ) mutant was identified from an ethyl methanesulfonate-mutagenized indica cultivar IR68 population and was previously shown to display a spontaneous cell death phenotype and enhanced resistance to rice fungal and bacterial pathogens .
Here , we have isolated Spl11 via a map-based cloning strategy .
The isolation of the Spl11 gene was facilitated by the identification of three additional spl11 alleles from an IR64 mutant collection .
The predicted SPL11 protein contains both a U-box domain and an armadillo ( ARM ) repeat domain , which were demonstrated in yeast and mammalian systems to be involved in ubiquitination and protein-protein interactions , respectively .
Amino acid sequence comparison indicated that the similarity between SPL11 and other plant U-box-ARM proteins is mostly restricted to the U-box and ARM repeat regions .
A single base substitution was detected in spl11 , which results in a premature stop codon in the SPL11 protein .
Expression analysis indicated that Spl11 is induced in both incompatible and compatible rice-blast interactions .
In vitro ubiquitination assay indicated that the SPL11 protein possesses E3 ubiquitin ligase activity that is dependent on an intact U-box domain , suggesting a role of the ubiquitination system in the control of plant cell death and defense . | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: The isolation of the Spl11 gene was facilitated by the identification of three additional spl11 alleles from an IR64 mutant collection .
[ Sen. 6, subscore: 2.00 ]: A single base substitution was detected in spl11 , which results in a premature stop codon in the SPL11 protein .
[ Sen. 1, subscore: 1.00 ]: The rice ( Oryza sativa ) spotted leaf11 ( spl11 ) mutant was identified from an ethyl methanesulfonate-mutagenized indica cultivar IR68 population and was previously shown to display a spontaneous cell death phenotype and enhanced resistance to rice fungal and bacterial pathogens .
[ Sen. 2, subscore: 1.00 ]: Here , we have isolated Spl11 via a map-based cloning strategy .
[ Sen. 4, subscore: 1.00 ]: The predicted SPL11 protein contains both a U-box domain and an armadillo ( ARM ) repeat domain , which were demonstrated in yeast and mammalian systems to be involved in ubiquitination and protein-protein interactions , respectively .
[ Sen. 5, subscore: 1.00 ]: Amino acid sequence comparison indicated that the similarity between SPL11 and other plant U-box-ARM proteins is mostly restricted to the U-box and ARM repeat regions .
[ Sen. 7, subscore: 1.00 ]: Expression analysis indicated that Spl11 is induced in both incompatible and compatible rice-blast interactions .
[ Sen. 8, subscore: 1.00 ]: In vitro ubiquitination assay indicated that the SPL11 protein possesses E3 ubiquitin ligase activity that is dependent on an intact U-box domain , suggesting a role of the ubiquitination system in the control of plant cell death and defense .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 10.00 |
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| Title: SPIN1 , a K Homology Domain Protein Negatively Regulated and Ubiquitinated by the E3 Ubiquitin Ligase SPL11 , Is Involved in Flowering Time Control in Rice .
| | Author: Vega-Sanchez ME Zeng L Chen S Leung H Wang GL | | Journal: Plant Cell Citation: V : 20 P : 1456-69 Year: 2008 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub18586868 Accession (PMID): 18586868 | | Abstract: The rice ( Oryza sativa ) E3 ligase SPOTTED LEAF11 ( SPL11 ) negatively regulates programmed cell death and disease resistance .
We demonstrate here that SPL11 also regulates flowering via interaction with SPIN1 ( for SPL11-interacting protein1 ) , a Signal Transduction and Activation of RNA family member .
SPIN1 binds RNA and DNA in vitro and interacts with SPL11 in the nucleus .
Spl11 mutants have delayed flowering under long-day conditions .
Spin1 overexpression causes late flowering independently of daylength ; expression analyses of flowering marker genes in these lines suggested that SPIN1 represses flowering by downregulating the flowering promoter gene Heading date3a ( Hd3a ) via Hd1-dependent mechanisms in short days and by targeting Hd1-independent factors in long days .
Both Spin1 and Spl11 are regulated diurnally in opposing phases .
SPL11 negatively regulates Spin1 transcript levels , while SPIN1 also affects Spl11 expression .
Moreover , we show that coincidence of high accumulation of Spin1 mRNA with the light in the morning and early evening is needed to repress flowering .
SPIN1 is monoubiquitinated by SPL11 , suggesting that it is not targeted for degradation .
Our data are consistent with a model in which SPIN1 acts as a negative regulator of flowering that itself is negatively regulated by SPL11 , possibly via ubiquitination .
| Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: We demonstrate here that SPL11 also regulates flowering via interaction with SPIN1 ( for SPL11-interacting protein1 ) , a Signal Transduction and Activation of RNA family member .
[ Sen. 7, subscore: 2.00 ]: SPL11 negatively regulates Spin1 transcript levels , while SPIN1 also affects Spl11 expression .
[ Sen. 1, subscore: 1.00 ]: The rice ( Oryza sativa ) E3 ligase SPOTTED LEAF11 ( SPL11 ) negatively regulates programmed cell death and disease resistance .
[ Sen. 3, subscore: 1.00 ]: SPIN1 binds RNA and DNA in vitro and interacts with SPL11 in the nucleus .
[ Sen. 4, subscore: 1.00 ]: Spl11 mutants have delayed flowering under long-day conditions .
[ Sen. 6, subscore: 1.00 ]: Both Spin1 and Spl11 are regulated diurnally in opposing phases .
[ Sen. 9, subscore: 1.00 ]: SPIN1 is monoubiquitinated by SPL11 , suggesting that it is not targeted for degradation .
[ Sen. 10, subscore: 1.00 ]: Our data are consistent with a model in which SPIN1 acts as a negative regulator of flowering that itself is negatively regulated by SPL11 , possibly via ubiquitination .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
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| Title: Fine genetic mapping and physical delimitation of the lesion mimic gene Spl11 to a 160-kb DNA segment of the rice genome .
| | Author: Zeng L Yin Z Chen J Leung H Wang GL .
| | Journal: Mol . Genet . Genomics Citation: V : 268 ( 2 ) P : 253-61 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12395200 Accession (PMID): 12395200 | | Abstract: The rice lesion mimic mutant spl11 was previously found to confer broad-spectrum disease resistance to both Magnaporthe grisea and Xanthomonas oryzae pv . oryzae .
To better understand the molecular basis underlying cell death and disease resistance in rice , a map-based cloning strategy has been employed to isolate Spl11 .
Five Spl11-linked RAPD markers were developed and four of them were mapped to rice chromosome 12 .
A high-resolution genetic map was developed using a segregating population consisting of 1138 lesion mimic individuals .
Recombination suppression was observed in the vicinity of Spl11 .
Three molecular markers tightly linked to Spl11 were identified and used to screen a BAC library .
A contig spanning the Spl11 locus was constructed and physical mapping delimited Spl11 to a 160-kb DNA segment within a single BAC clone .
These results provide the essential information for the final isolation of this important gene in the rice defense pathway . | Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: A contig spanning the Spl11 locus was constructed and physical mapping delimited Spl11 to a 160-kb DNA segment within a single BAC clone .
[ Sen. 1, subscore: 1.00 ]: The rice lesion mimic mutant spl11 was previously found to confer broad-spectrum disease resistance to both Magnaporthe grisea and Xanthomonas oryzae pv . oryzae .
[ Sen. 2, subscore: 1.00 ]: To better understand the molecular basis underlying cell death and disease resistance in rice , a map-based cloning strategy has been employed to isolate Spl11 .
[ Sen. 3, subscore: 1.00 ]: Five Spl11-linked RAPD markers were developed and four of them were mapped to rice chromosome 12 .
[ Sen. 5, subscore: 1.00 ]: Recombination suppression was observed in the vicinity of Spl11 .
[ Sen. 6, subscore: 1.00 ]: Three molecular markers tightly linked to Spl11 were identified and used to screen a BAC library .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
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| Title: The U-box E3 Ligase SPL11/PUB13 Is a Convergence Point of Defense and Flowering Signaling in Plants .
| | Author: Liu J Li W Ning Y Shirsekar G Wang X Dai L Wang Z Liu W Wang GL | | Journal: Plant Physiol Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22659522 Accession (PMID): 22659522 | | Abstract: Plants use the ubiquitin-proteasome system ( UPS ) to regulate nearly every aspect of growth and development and to respond to abiotic and biotic stresses .
Among the three major enzymes involved in the UPS , E3 ligases determine substrate specificity and actively participate in many biological processes in plants .
Emerging evidence shows that some E3 ligases have multiple functions and serve as a connection node in plant signaling .
Here , we review the dual functions of the U-box and armadillo ( ARM ) repeat domain E3 ligase SPL11 in rice and of its ortholog PUB13 in Arabidopsis in modulating innate immunity and flowering .
Both SPL11 and PUB13 negatively regulate programmed cell death ( PCD ) and defense .
Intriguingly , SPL11 promotes flowering under long-day ( LD ) conditions in rice while PUB13 suppresses flowering under LD conditions in Arabidopsis .
SPL11 regulates defense through a putative GAP protein and regulates flowering through an RNA-binding protein .
PUB13 modulates defense through FLS2 and may control flowering through HFR1 .
Moreover , PUB13-mediated defense and flowering depend on the plant hormone salicylic acid ( SA ) .
The similar functions of SPL11 and PUB13 , and the complementation of the pub13 cell death and flowering phenotypes by Spl11 indicate that Spl11/PUB13 is an ancient , functionally conserved locus in monocot and dicot plants .
In the process of speciation , the downstream signaling components have , however , diversified in these two species .
We conclude by proposing working models of how SPL11 and PUB13 and their associated proteins modulate both defense and flowering in monocot and dicot plants .
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[ Sen. 10, subscore: 2.00 ]: The similar functions of SPL11 and PUB13 , and the complementation of the pub13 cell death and flowering phenotypes by Spl11 indicate that Spl11/PUB13 is an ancient , functionally conserved locus in monocot and dicot plants .
[ Sen. 4, subscore: 1.00 ]: Here , we review the dual functions of the U-box and armadillo ( ARM ) repeat domain E3 ligase SPL11 in rice and of its ortholog PUB13 in Arabidopsis in modulating innate immunity and flowering .
[ Sen. 5, subscore: 1.00 ]: Both SPL11 and PUB13 negatively regulate programmed cell death ( PCD ) and defense .
[ Sen. 6, subscore: 1.00 ]: Intriguingly , SPL11 promotes flowering under long-day ( LD ) conditions in rice while PUB13 suppresses flowering under LD conditions in Arabidopsis .
[ Sen. 7, subscore: 1.00 ]: SPL11 regulates defense through a putative GAP protein and regulates flowering through an RNA-binding protein .
[ Sen. 12, subscore: 1.00 ]: We conclude by proposing working models of how SPL11 and PUB13 and their associated proteins modulate both defense and flowering in monocot and dicot plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
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| Title: Physical mapping of a rice lesion mimic gene , Spl1 , to a 70-kb segment of rice chromosome 12 .
| | Author: Liu G Wang L Zhou Z Leung H Wang GL He C | | Journal: Mol . Genet . Genomics Citation: V : 272 ( 1 ) P : 108-15 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15258851 Accession (PMID): 15258851 | | Abstract: The rice lesion mimic mutant spotted leaf 1 ( spl1 ) was first identified in the rice ( Oryza sativa ) cultivar Asahi in 1965 .
This mutant displayed spontaneous disease-like lesions in the absence of any pathogen , and was found to confer resistance to multiple isolates of rice blast We employed a map-based cloning strategy to localize the Spl1 gene .
A total of ten cleaved amplified polymorphic sequence ( CAPS ) markers linked to the Spl1 gene were identified and mapped to an 8 . 5-cM region on chromosome 12 .
A high-resolution genetic map was developed using these ten CAPS markers and a segregating population consisting of 3202 individuals .
A BAC contig containing four BAC clones was constructed , and Spl1 was localized to a 423-kb region .
Seven spl1 mutants were obtained from the IR64 deletion mutant collection , and molecular analysis using these mutants delimited the Spl1 gene to a 70-kb interval , covered by two BAC clones .
These results provide the basis for cloning this gene , which is involved in cell death and disease resistance in rice . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Seven spl1 mutants were obtained from the IR64 deletion mutant collection , and molecular analysis using these mutants delimited the Spl1 gene to a 70-kb interval , covered by two BAC clones .
[ Sen. 1, subscore: 1.00 ]: The rice lesion mimic mutant spotted leaf 1 ( spl1 ) was first identified in the rice ( Oryza sativa ) cultivar Asahi in 1965 .
[ Sen. 2, subscore: 1.00 ]: This mutant displayed spontaneous disease-like lesions in the absence of any pathogen , and was found to confer resistance to multiple isolates of rice blast We employed a map-based cloning strategy to localize the Spl1 gene .
[ Sen. 3, subscore: 1.00 ]: A total of ten cleaved amplified polymorphic sequence ( CAPS ) markers linked to the Spl1 gene were identified and mapped to an 8 . 5-cM region on chromosome 12 .
[ Sen. 5, subscore: 1.00 ]: A BAC contig containing four BAC clones was constructed , and Spl1 was localized to a 423-kb region .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
