| 8 matches found in 3 documents. Search time: 0.029 seconds. |
|---|
|
Score: 4.00 |
|---|
| Title: The combination of a novel stimulatory element in the first exon of the maize Shrunken-1 gene with the following intron 1 enhances reporter gene expression up to 1000-fold .
| | Author: Maas C Laufs J Grant S Korfhage C Werr W | | Journal: Plant Mol . Biol . Citation: V : 16 ( 2 ) P : 199-207 Year: 1991 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1893097 Accession (PMID): 1893097 | | Abstract: Both exon 1 and intron 1 of the maize Shrunken-1 ( Sh1 ) gene individually stimulate expression of reporter genes in transient gene expression experiments if present within the transcription unit .
The Sh1 exon 1 mediates a 10-fold increase in activity when inserted at the 5 end of the bacterial chloramphenicol transacetylase ( CAT ) marker gene in both monocot and dicot protoplasts .
The Sh1 intron 1 enhances chimeric gene expression in rice and maize protoplasts approximately 100-fold but inhibits CAT expression in tobacco protoplasts .
In combination , the stimulatory effects of Sh1 exon 1 and intron 1 are multiplicative in monocot protoplasts resulting in a final enhancement of up to 1000-fold compared to the unmodified CAT or luciferase marker genes .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Both exon 1 and intron 1 of the maize Shrunken-1 ( Sh1 ) gene individually stimulate expression of reporter genes in transient gene expression experiments if present within the transcription unit .
[ Sen. 2, subscore: 1.00 ]: The Sh1 exon 1 mediates a 10-fold increase in activity when inserted at the 5 end of the bacterial chloramphenicol transacetylase ( CAT ) marker gene in both monocot and dicot protoplasts .
[ Sen. 3, subscore: 1.00 ]: The Sh1 intron 1 enhances chimeric gene expression in rice and maize protoplasts approximately 100-fold but inhibits CAT expression in tobacco protoplasts .
[ Sen. 4, subscore: 1.00 ]: In combination , the stimulatory effects of Sh1 exon 1 and intron 1 are multiplicative in monocot protoplasts resulting in a final enhancement of up to 1000-fold compared to the unmodified CAT or luciferase marker genes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| Title: Parallel domestication of the Shattering1 genes in cereals .
| | Author: Lin Z Li X Shannon LM Yeh CT Wang ML Bai G Peng Z Li J Trick HN Clemente TE Doebley J Schnable PS Tuinstra MR Tesso TT White F Yu J | | Journal: Nat Genet Citation: V : 44 P : 720-4 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22581231 Accession (PMID): 22581231 | | Abstract: A key step during crop domestication is the loss of seed shattering .
Here , we show that seed shattering in sorghum is controlled by a single gene , Shattering1 ( Sh1 ) , which encodes a YABBY transcription factor .
Domesticated sorghums harbor three different mutations at the Sh1 locus .
Variants at regulatory sites in the promoter and intronic regions lead to a low level of expression , a 2 . 2-kb deletion causes a truncated transcript that lacks exons 2 and 3 , and a GT-to-GG splice-site variant in the intron 4 results in removal of the exon 4 .
The distributions of these non-shattering haplotypes among sorghum landraces suggest three independent origins .
The function of the rice ortholog ( OsSh1 ) was subsequently validated with a shattering-resistant mutant , and two maize orthologs ( ZmSh1-1 and ZmSh1-5 . 1+ZmSh1-5 . 2 ) were verified with a large mapping population .
Our results indicate that Sh1 genes for seed shattering were under parallel selection during sorghum , rice and maize domestication .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Here , we show that seed shattering in sorghum is controlled by a single gene , Shattering1 ( Sh1 ) , which encodes a YABBY transcription factor .
[ Sen. 3, subscore: 1.00 ]: Domesticated sorghums harbor three different mutations at the Sh1 locus .
[ Sen. 7, subscore: 1.00 ]: Our results indicate that Sh1 genes for seed shattering were under parallel selection during sorghum , rice and maize domestication .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: One genes shattering effects .
| | Author: Olsen KM | | Journal: Nat Genet Citation: V : 44 P : 616-7 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22641204 Accession (PMID): 22641204 | | Abstract: A new study shows that three independent mutations in the Sh1 gene , which encodes a YABBY transcription factor , gave rise to the non-shattering seed phenotype in domesticated sorghum .
This same gene may have also had a role in the domestication of other cereals , including maize and rice .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A new study shows that three independent mutations in the Sh1 gene , which encodes a YABBY transcription factor , gave rise to the non-shattering seed phenotype in domesticated sorghum .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
