| 24 matches found in 9 documents. Search time: 1.019 seconds. |
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Score: 9.00 |
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| Title: RNAi knockdown of rice SE5 gene is sensitive to the herbicide methyl viologen by the down-regulation of antioxidant defense .
| | Author: Xu S Wang L Zhang B Han B Xie Y Yang J Zhong W Chen H Wang R Wang N Cui W Shen W | | Journal: Plant Mol Biol Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22829206 Accession (PMID): 22829206 | | Abstract: Plant heme oxygenase ( HO ) catalyzes the oxygenation of heme to biliverdin , carbon monoxide ( CO ) , and free iron ( Fe ( 2+ ) ) -and Arabidopsis and rice ( Oryza sativa ) HOs are involved in light signaling .
Here , we identified that the rice PHOTOPERIOD SENSITIVITY 5 ( SE5 ) gene , which encoded a putative HO with high similarity to HO-1 from Arabidopsis ( HY1 ) , exhibited HO activity , and localized in the chloroplasts .
Rice RNAi mutants silenced for SE5 were generated and displayed early flowering under long-day conditions , consistent with phenotypes of the null mutation in SE5 gene reported previously ( se5 and s73 ) .
The herbicide methyl viologen ( MV ) , which produces reactive oxygen species ( ROS ) , was applied to determine whether SE5 regulates oxidative stress response .
Compared with wild-type , SE5 RNAi transgenic plants aggravated seedling growth inhibition , chlorophyll loss and ROS overproduction , and decreased the transcripts of some representative antioxidative genes .
By contrast , administration of exogenous CO partially rescued corresponding MV hypersensitivity in the SE5 RNAi plants .
Alleviation of seed germination inhibition , chlorophyll loss and ROS overproduction , as well as the induction of antioxidant defense were further observed when SE5 or HY1 was overexpressed in transgenic Arabidopsis plants , indicating that SE5 may be useful for molecular breeding designed to improve plant tolerance to oxidative stress .
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[ Sen. 3, subscore: 3.00 ]: Rice RNAi mutants silenced for SE5 were generated and displayed early flowering under long-day conditions , consistent with phenotypes of the null mutation in SE5 gene reported previously ( se5 and s73 ) .
[ Sen. 7, subscore: 2.00 ]: Alleviation of seed germination inhibition , chlorophyll loss and ROS overproduction , as well as the induction of antioxidant defense were further observed when SE5 or HY1 was overexpressed in transgenic Arabidopsis plants , indicating that SE5 may be useful for molecular breeding designed to improve plant tolerance to oxidative stress .
[ Sen. 2, subscore: 1.00 ]: Here , we identified that the rice PHOTOPERIOD SENSITIVITY 5 ( SE5 ) gene , which encoded a putative HO with high similarity to HO-1 from Arabidopsis ( HY1 ) , exhibited HO activity , and localized in the chloroplasts .
[ Sen. 4, subscore: 1.00 ]: The herbicide methyl viologen ( MV ) , which produces reactive oxygen species ( ROS ) , was applied to determine whether SE5 regulates oxidative stress response .
[ Sen. 5, subscore: 1.00 ]: Compared with wild-type , SE5 RNAi transgenic plants aggravated seedling growth inhibition , chlorophyll loss and ROS overproduction , and decreased the transcripts of some representative antioxidative genes .
[ Sen. 6, subscore: 1.00 ]: By contrast , administration of exogenous CO partially rescued corresponding MV hypersensitivity in the SE5 RNAi plants .
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Score: 7.00 |
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| Title: Phytochromes confer the photoperiodic control of flowering in rice ( a short-day plant ) .
| | Author: Izawa T Oikawa T Tokutomi S Okuno K Shimamoto K | | Journal: Plant J Citation: V : 22 ( 5 ) P : 391-9 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10849355 Accession (PMID): 10849355 | | Abstract: The photoperiodic sensitivity 5 ( se5 ) mutant of rice , a short-day plant , has a very early flowering phenotype and is completely deficient in photoperiodic response .
We have cloned the SE5 gene by candidate cloning and demonstrated that it encodes a putative heme oxygenase .
Lack of responses of coleoptile elongation by light pulses and photoreversible phytochromes in crude extracts of se5 indicate that SE5 may function in phytochrome chromophore biosynthesis .
Ectopic expression of SE5 cDNA by the CaMV 35S promoter restored the photoperiodic response in the se5 mutant .
Our results indicate that phytochromes confer the photoperiodic control of flowering in rice .
Comparison of se5 with hy1 , a counterpart mutant of Arabidopsis , suggests distinct roles of phytochromes in the photoperiodic control of flowering in these two species . | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: Lack of responses of coleoptile elongation by light pulses and photoreversible phytochromes in crude extracts of se5 indicate that SE5 may function in phytochrome chromophore biosynthesis .
[ Sen. 4, subscore: 2.00 ]: Ectopic expression of SE5 cDNA by the CaMV 35S promoter restored the photoperiodic response in the se5 mutant .
[ Sen. 1, subscore: 1.00 ]: The photoperiodic sensitivity 5 ( se5 ) mutant of rice , a short-day plant , has a very early flowering phenotype and is completely deficient in photoperiodic response .
[ Sen. 2, subscore: 1.00 ]: We have cloned the SE5 gene by candidate cloning and demonstrated that it encodes a putative heme oxygenase .
[ Sen. 6, subscore: 1.00 ]: Comparison of se5 with hy1 , a counterpart mutant of Arabidopsis , suggests distinct roles of phytochromes in the photoperiodic control of flowering in these two species .
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Score: 4.00 |
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| Title: Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method .
| | Author: Hayama R Izawa T Shimamoto K | | Journal: Plant Cell Physiol .
Citation: V : 43 ( 5 ) P : 494-504 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12040096 Accession (PMID): 12040096 | | Abstract: To better understand the molecular mechanisms of the photoperiodic regulation of rice , a short-day plant , we isolated 27 cDNAs that were differentially expressed in the photoperiod-insensitive se5 mutant from approximately 8 , 400 independent mRNA species by the use of a fluorescent differential display ( FDD ) .
For this screening , we isolated mRNAs at five different time points during the night and compared their expression patterns between se5 and the wild type .
Of 27 cDNAs isolated , 12 showed diurnal expression patterns often associated with genes involved in the determination of the flowering time .
In se5 , expression of nine cDNAs was increased .
Five of these cDNAs were up-regulated under SD , suggesting that they may promote flowering under SD .
They included genes encoding a cDNA containing a putative NAC domain , the fructose-bisphosphate aldolase , and a protease inhibitor .
Expression of three cDNAs was decreased in se5 but not photoperiodically regulated .
These cDNAs included a rice homolog of Arabidopsis GIGANTEA ( GI ) , lir1 , and a gene for myo-inositol 1-phosphate synthase , all of which were previously shown to be under the control of circadian clocks .
The expression patterns of the rice homolog of GI , OsGI , were similar to those of the Arabidopsis GI , suggesting the conservation of some mechanisms for the photoperiodic regulation of flowering between these two species . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: To better understand the molecular mechanisms of the photoperiodic regulation of rice , a short-day plant , we isolated 27 cDNAs that were differentially expressed in the photoperiod-insensitive se5 mutant from approximately 8 , 400 independent mRNA species by the use of a fluorescent differential display ( FDD ) .
[ Sen. 2, subscore: 1.00 ]: For this screening , we isolated mRNAs at five different time points during the night and compared their expression patterns between se5 and the wild type .
[ Sen. 4, subscore: 1.00 ]: In se5 , expression of nine cDNAs was increased .
[ Sen. 7, subscore: 1.00 ]: Expression of three cDNAs was decreased in se5 but not photoperiodically regulated .
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Score: 4.00 |
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| Title: Analysis of PHOTOPERIOD SENSITIVITY5 sheds light on the role of phytochromes in photoperiodic flowering in rice .
| | Author: Andres F Galbraith DW Talon M Domingo C | | Journal: Plant Physiol Citation: V : 151 P : 681-90 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19675157 Accession (PMID): 19675157 | | Abstract: A great number of plants synchronize flowering with day length .
In rice ( Oryza sativa ) , photoperiod is the primary environmental cue that triggers flowering .
Here , we show that the s73 mutant , identified in a gamma-irradiated Bahia collection , displays early flowering and photoperiodic insensitivity due to a null mutation in the PHOTOPERIOD SENSITIVITY5 ( SE5 ) gene , which encodes an enzyme implicated in phytochrome chromophore biosynthesis . s73 mutant plants show a number of alterations in the characteristic diurnal expression patterns of master genes involved in photoperiodic control of flowering , resulting in up-regulation of the floral integrator Heading date3a ( Hd3a ) .
Early heading date1 ( Ehd1 ) , an additional rice floral activator , was also highly expressed in the s73 mutant , suggesting that SE5 represses Ehd1 in wild-type plants .
Silencing of Ehd1 in both Bahia and s73 backgrounds indicated that SE5 regulates Ehd1 expression .
The data also indicate that SE5 confers photoperiodic sensitivity through regulation of Hd1 .
These results provide direct evidence that phytochromes inhibit flowering by affecting both Hd1 and Ehd1 flowering pathways .
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[ Sen. 3, subscore: 1.00 ]: Here , we show that the s73 mutant , identified in a gamma-irradiated Bahia collection , displays early flowering and photoperiodic insensitivity due to a null mutation in the PHOTOPERIOD SENSITIVITY5 ( SE5 ) gene , which encodes an enzyme implicated in phytochrome chromophore biosynthesis . s73 mutant plants show a number of alterations in the characteristic diurnal expression patterns of master genes involved in photoperiodic control of flowering , resulting in up-regulation of the floral integrator Heading date3a ( Hd3a ) .
[ Sen. 4, subscore: 1.00 ]: Early heading date1 ( Ehd1 ) , an additional rice floral activator , was also highly expressed in the s73 mutant , suggesting that SE5 represses Ehd1 in wild-type plants .
[ Sen. 5, subscore: 1.00 ]: Silencing of Ehd1 in both Bahia and s73 backgrounds indicated that SE5 regulates Ehd1 expression .
[ Sen. 6, subscore: 1.00 ]: The data also indicate that SE5 confers photoperiodic sensitivity through regulation of Hd1 .
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Score: 2.00 |
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| Title: The Histone Methyltransferase SDG724 Mediates H3K36me2/3 Deposition at MADS50 and RFT1 and Promotes Flowering in Rice .
| | Author: Sun C Fang J Zhao T Xu B Zhang F Liu L Tang J Zhang G Deng X Chen F Qian Q Cao X Chu C | | Journal: Plant Cell Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22892321 Accession (PMID): 22892321 | | Abstract: Chromatin modifications affect flowering time in the long-day plant Arabidopsis thaliana , but the role of histone methylation in flowering time regulation of rice ( Oryza sativa ) , a short-day plant , remains to be elucidated .
We identified a late-flowering long vegetative phase1 ( lvp1 ) mutant in rice and used map-based cloning to reveal that lvp1 affects the SET domain group protein 724 ( SDG724 ) .
SDG724 functions as a histone methyltransferase in vitro and contributes to a major fraction of global histone H3 lysine 36 ( H3K36 ) methylation in vivo .
Expression analyses of flowering time genes in wild-type and lvp1 mutants revealed that Early heading date1 , but not Heading date1 , are misregulated in lvp1 mutants .
In addition , the double mutant of lvp1 with photoperiod sensitivity5 ( se5 ) flowered later than the se5 single mutant , indicating that lvp1 delays flowering time irrespective of photoperiod .
Chromatin immunoprecipitation assays showed that lvp1 had reduced levels of H3K36me2/3 at MADS50 and RFT1 .
This suggests that the divergent functions of paralogs RFT1 and Hd3a , and of MADS50 and MADS51 , are in part due to differential H3K36me2/3 deposition , which also correlates with higher expression levels of MADS50 and RFT1 in flowering promotion in rice .
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[ Sen. 5, subscore: 2.00 ]: In addition , the double mutant of lvp1 with photoperiod sensitivity5 ( se5 ) flowered later than the se5 single mutant , indicating that lvp1 delays flowering time irrespective of photoperiod .
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