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| Title: Complete loss of photoperiodic response in the rice mutant line X61 is caused by deficiency of phytochrome chromophore biosynthesis gene .
| | Author: Saito H Okumoto Y Yoshitake Y Inoue H Yuan Q Teraishi M Tsukiyama T Nishida H Tanisaka T | | Journal: Theor Appl Genet Citation: V : 122 P : 109-18 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20700573 Accession (PMID): 20700573 | | Abstract: In rice ( Oryza sativa ) , a short-day plant , photoperiod is the most favorable external signal for floral induction because of the constant seasonal change throughout the years .
Compared with Arabidopsis , however , a large part of the regulation mechanism of the photoperiodic response in rice still remains unclear due mainly to the lack of induced mutant genes .
An induced mutant line X61 flowers 35 days earlier than its original variety Gimbozu under a natural photoperiod in Kyoto ( 35 degrees 01N ) .
We attempted to identify the mutant gene conferring early heading to X61 .
Experimental results showed that the early heading of X61 was conferred by a complete loss of photoperiodic response due to a novel single recessive mutant gene se13 .
This locus interacts with two crucial photoperiod sensitivity loci , Se1 and E1 .
Wild type alleles at these two loci do not function in coexistence with se13 in a homozygous state , suggesting that Se13 is an upstream locus of the Se1 and E1 loci .
Linkage analysis showed that Se13 is located in a 110 kb region between the two markers , INDEL3735_1 and INDEL3735_3 on chromosome 1 .
A database search suggested that the Se13 gene is identical to AK101395 ( =OsHY2 ) , which encodes phytochromobilin synthase , a key enzyme in phytochrome chromophore biosynthesis .
Subsequent sequence analysis revealed that X61 harbors a 1 bp insertion in exon 1 of OsHY2 , which induces a frame-shift mutation producing a premature stop codon .
It is therefore considered that the complete loss of photoperiodic response of X61 is caused by a loss of function of the Se13 ( OsHY2 ) gene involved in phytochrome chromophore biosynthesis .
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[ Sen. 7, subscore: 2.00 ]: Wild type alleles at these two loci do not function in coexistence with se13 in a homozygous state , suggesting that Se13 is an upstream locus of the Se1 and E1 loci .
[ Sen. 5, subscore: 1.00 ]: Experimental results showed that the early heading of X61 was conferred by a complete loss of photoperiodic response due to a novel single recessive mutant gene se13 .
[ Sen. 8, subscore: 1.00 ]: Linkage analysis showed that Se13 is located in a 110 kb region between the two markers , INDEL3735_1 and INDEL3735_3 on chromosome 1 .
[ Sen. 9, subscore: 1.00 ]: A database search suggested that the Se13 gene is identical to AK101395 ( =OsHY2 ) , which encodes phytochromobilin synthase , a key enzyme in phytochrome chromophore biosynthesis .
[ Sen. 11, subscore: 1.00 ]: It is therefore considered that the complete loss of photoperiodic response of X61 is caused by a loss of function of the Se13 ( OsHY2 ) gene involved in phytochrome chromophore biosynthesis .
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