| 18 matches found in 9 documents. Search time: 0.162 seconds. |
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Score: 5.00 |
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| Title: Molecular aspect of good eating quality formation in Japonica rice .
| | Author: Sun MM Abdula SE Lee HJ Cho YC Han LZ Koh HJ Cho YG | | Journal: PLoS One Citation: V : 6 P : e18385 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21494675 Accession (PMID): 21494675 | | Abstract: The composition of amylopectin is the determinant of rice eating quality under certain threshold of protein content and the ratio of amylose and amylopectin .
In molecular biology level , the fine structure of amylopectin is determined by relative activities of starch branching enzyme ( SBE ) , granule-bound starch synthase ( GBSS ) , and soluble starch synthase ( SSS ) in rice grain under the same ADP-Glucose level .
But the underlying mechanism of eating quality in molecular biology level remains unclear .
This paper reports the differences on major parameters such as SNP and insertion-deletion sites , RNA expressions , and enzyme activities associated with eating quality of japonica varieties .
Eight japonica rice varieties with significant differences in various eating quality parameters such as palatability and protein content were used in this experiment .
Association analysis between nucleotide polymorphism and eating quality showed that S12 and S13 loci in SBE1 , S55 in SSS1 , S58 in SSS2A were significantly associated with apparent amylose content , alkali digestion value , setback viscosity , consistency viscosity , pasting temperature , which explained most of the variation in apparent amylose content , setback viscosity , and consistency viscosity ; and explained almost all variations in alkali digestion value and pasting temperature .
Thirty-five SNPs and insertion-deletions from SBE1 , SBE3 , GBSS1 , SSS1 , and SSS2A differentiated high or intermediate palatability rice varieties from low palatability rice varieties .
Correlation analysis between enzyme activities and eating quality properties revealed that SBE25 and SSS15/W15 were positively correlated with palatability , whereas GBSS10 and GBSS15 were negatively correlated .
Gene expressions showed that SBE1 and SBE3 expressions in high palatability varieties tended to be higher than middle and low palatability varieties .
Collectively , SBE1 , SBE3 , SSS1 , and SSS2A , especially SBE1 and SBE3 could improve eating quality , but GBSS1 decreased eating quality .
The results indicated the possibility of developing high palatability cultivars through modification of key genes related to japonica rice eating quality formation in starch biosynthesis .
| Matching Sentences:
[ Sen. 10, subscore: 2.00 ]: Collectively , SBE1 , SBE3 , SSS1 , and SSS2A , especially SBE1 and SBE3 could improve eating quality , but GBSS1 decreased eating quality .
[ Sen. 6, subscore: 1.00 ]: Association analysis between nucleotide polymorphism and eating quality showed that S12 and S13 loci in SBE1 , S55 in SSS1 , S58 in SSS2A were significantly associated with apparent amylose content , alkali digestion value , setback viscosity , consistency viscosity , pasting temperature , which explained most of the variation in apparent amylose content , setback viscosity , and consistency viscosity ; and explained almost all variations in alkali digestion value and pasting temperature .
[ Sen. 7, subscore: 1.00 ]: Thirty-five SNPs and insertion-deletions from SBE1 , SBE3 , GBSS1 , SSS1 , and SSS2A differentiated high or intermediate palatability rice varieties from low palatability rice varieties .
[ Sen. 9, subscore: 1.00 ]: Gene expressions showed that SBE1 and SBE3 expressions in high palatability varieties tended to be higher than middle and low palatability varieties .
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Score: 4.00 |
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| Title: Molecular analysis of the gene encoding a rice starch branching enzyme .
| | Author: Kawasaki T Mizuno K Baba T Shimada H | | Journal: Mol . Gen . Genet . Citation: V : 237 ( 1-2 ) P : 10-6 Year: 1993 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8455548 Accession (PMID): 8455548 | | Abstract: The sequence of a rice gene encoding a starch branching enzyme ( sbe1 ) shows extreme divergence from that of the rice gene , that is homologous to bacterial glycogen branching enzyme ( sbe2 ) . sbe1 is expressed abundantly and specifically in developing seeds and maximally in the middle stages of seed development .
This expression pattern completely coincides with that of the waxy gene , which encodes a granule-bound starch synthase .
Three G-box motifs and consensus promoter sequences are present in the 5 flanking region of sbe1 .
It encodes a putative transit peptide , which is required for transport into the amyloplast A 2 . 2 kb intron ( intron 2 ) precedes the border between the regions encoding the transit peptide and the mature protein , and contains a high G/C content with several repeated sequences in its 5 half .
Although only a single copy of sbe1 is present in the rice genome , Southern analysis using intron 2 as a probe indicates the presence of several homologous sequences in the rice genome , suggesting that this large intron and also the transit peptide coding region may be acquired from another portion of the genome by duplication and insertion of the sequence into the gene . | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: The sequence of a rice gene encoding a starch branching enzyme ( sbe1 ) shows extreme divergence from that of the rice gene , that is homologous to bacterial glycogen branching enzyme ( sbe2 ) . sbe1 is expressed abundantly and specifically in developing seeds and maximally in the middle stages of seed development .
[ Sen. 3, subscore: 1.00 ]: Three G-box motifs and consensus promoter sequences are present in the 5 flanking region of sbe1 .
[ Sen. 5, subscore: 1.00 ]: Although only a single copy of sbe1 is present in the rice genome , Southern analysis using intron 2 as a probe indicates the presence of several homologous sequences in the rice genome , suggesting that this large intron and also the transit peptide coding region may be acquired from another portion of the genome by duplication and insertion of the sequence into the gene .
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Score: 3.00 |
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| Title: Starch-branching enzyme I-deficient mutation specifically affects the structure and properties of starch in rice endosperm .
| | Author: Satoh H Nishi A Yamashita K Takemoto Y Tanaka Y Hosaka Y Sakurai A Fujita N Nakamura Y | | Journal: Plant Physiol .
Citation: V : 133 ( 3 ) P : 1111-21 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14526120 Accession (PMID): 14526120 | | Abstract: We have isolated a starch mutant that was deficient in starch-branching enzyme I ( BEI ) from the endosperm mutant stocks of rice ( Oryza sativa ) induced by the treatment of fertilized egg cells with N-methyl-N-nitrosourea .
The deficiency of BEI in this mutant was controlled by a single recessive gene , tentatively designated as starch-branching enzyme mutant 1 ( sbe1 ) .
The mutant endosperm exhibited the normal phenotype and contained the same amount of starch as the wild type .
However , the mutation apparently altered the fine structure of amylopectin .
The mutant amylopectin was characterized by significant decrease in both long chains with degree of polymerization ( DP ) > or = 37 and short chains with DP 12 to 21 , marked increase in short chains with DP < or = 10 ( A chains ) , and slight increase in intermediate chains with DP 24 to 34 , suggesting that BEI specifically synthesizes B1 and B2-3 chains .
The endosperm starch from the sbe1 mutant had a lower onset concentration for urea gelatinization and a lower onset temperature for thermo-gelatinization compared with the wild type , indicating that the genetic modification of amylopectin fine structure is responsible for changes in physicochemical properties of sbe1 starch . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: The endosperm starch from the sbe1 mutant had a lower onset concentration for urea gelatinization and a lower onset temperature for thermo-gelatinization compared with the wild type , indicating that the genetic modification of amylopectin fine structure is responsible for changes in physicochemical properties of sbe1 starch .
[ Sen. 2, subscore: 1.00 ]: The deficiency of BEI in this mutant was controlled by a single recessive gene , tentatively designated as starch-branching enzyme mutant 1 ( sbe1 ) .
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Score: 3.00 |
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| Title: Microsatellites , single nucleotide polymorphisms and a sequence tagged site in starch-synthesizing genes in relation to starch physicochemical properties in nonwaxy rice ( Oryza sativa L ) .
| | Author: Bao JS Corke H Sun M | | Journal: Theor . Appl . Genet . Citation: V : 113 ( 7 ) P : 1185-96 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16964521 Accession (PMID): 16964521 | | Abstract: Starch characteristics determine the quality of various products of rice , eg , eating , cooking and processing qualities .
Our previous study indicated that molecular markers inside or close to starch synthesizing genes can differentiate the starch properties of 56 waxy rices .
Here we report microsatellite ( or simple sequence repeat , SSR ) polymorphism in the Waxy ( Wx ) gene , soluble starch synthase I gene ( SS1 ) and starch branching enzyme 1 gene ( SBE1 ) , single nucleotide polymorphism ( SNP ) in Wx and starch branching enzyme 3 gene ( SBE3 ) , and a sequence tagged site ( STS ) in starch branching enzyme 1 gene ( SBE1 ) among 499 nonwaxy rice samples and their relationships with starch physicochemical properties .
The nonwaxy rice samples consist of landraces ( n = 172 ) obtained from germplasm centers and cultivars and breeding lines ( n = 327 ) obtained from various breeding programs .
Ten ( CT ) ( n ) microsatellite alleles , ( CT ) ( 8 ) , ( CT ) ( 10 ) , ( CT ) ( 11 ) , ( CT ) ( 12 , ) ( CT ) ( 17 ) , ( CT ) ( 18 ) , ( CT ) ( 19 ) , ( CT ) ( 20 ) , ( CT ) ( 21 ) , and ( CT ) ( 22 ) , were found at the Wx locus , of which ( CT ) ( 11 ) was the most frequent , and ( CT ) ( 12 ) , ( CT ) ( 21 ) and ( CT ) ( 22 ) were identified for the first time .
Four ( CT ) ( n ) microsatellite alleles were found at the SBE1 locus , ( CT ) ( 8 ) , ( CT ) ( 9 ) , and ( CT ) ( 10 ) together with an insertion sequence of CTCTCGGGCGA , and ( CT ) ( 8 ) alone without the insertion , of which ( CT ) ( 9 ) and the insertion was a new allele identified in only one rice , IR1552 .
Multiple microsatellites clustered at the SS1 locus , and in addition to the three alleles previously detected ( SSS-A = ( AC ) ( 2 ) . . . TCC ( TC ) ( 11 ) . . . ( TC ) ( 5 ) C ( ACC ) ( 11 ) , SSS-B = ( AC ) ( 3 ) . . . TCT ( TC ) ( 6 ) . . . ( TC ) ( 4 ) C ( ACC ) ( 9 ) , and SSS-C = ( AC ) ( 3 ) . . . TCT ( TC ) ( 6 ) . . . ( TC ) ( 4 ) C ( ACC ) ( 8 ) ) , one new allele ( SSS-D = ( AC ) ( 2 ) . . . TCC ( TC ) ( 10 ) . . . ( TC ) ( 4 ) C ( ACC ) ( 9 ) ) was found .
Analysis of the starch physicochemical properties of the samples with different microsatellites , SNPs and STS groups indicated that these molecular markers can differentiate almost all the physicochemical properties examined , eg , apparent amylose content ( AAC ) , pasting viscosity characteristics , and gel textural properties .
Wx SSR and Wx SNP alone explained more variations for all physicochemical properties than the other molecular markers .
The total six markers could explain 92 . 2 , 81 and 86% of total variation of AAC , gel hardness ( HD ) , and gel cohesiveness ( COH ) , respectively , and they could explain more than 40% of the total variation of hot paste viscosity ( HPV ) , cool paste viscosity ( CPV ) , breakdown viscosity ( BD ) , setback viscosity ( SB ) and gel adhesiveness ( ADH ) .
However , only 29% of the total variation of peak viscosity ( PV ) and 37% of pasting temperature ( PT ) could be explained by all the molecular markers .
Some of these markers can differentiate the starch physicochemical properties among the rice samples with the same Wx allele , indicating that the variation within Wx allele classes can be explained by other starch synthesizing genes .
These SSRs , SNPs and STS are useful in marker-assisted breeding for the improvement of starch quality of rice . | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: Here we report microsatellite ( or simple sequence repeat , SSR ) polymorphism in the Waxy ( Wx ) gene , soluble starch synthase I gene ( SS1 ) and starch branching enzyme 1 gene ( SBE1 ) , single nucleotide polymorphism ( SNP ) in Wx and starch branching enzyme 3 gene ( SBE3 ) , and a sequence tagged site ( STS ) in starch branching enzyme 1 gene ( SBE1 ) among 499 nonwaxy rice samples and their relationships with starch physicochemical properties .
[ Sen. 6, subscore: 1.00 ]: Four ( CT ) ( n ) microsatellite alleles were found at the SBE1 locus , ( CT ) ( 8 ) , ( CT ) ( 9 ) , and ( CT ) ( 10 ) together with an insertion sequence of CTCTCGGGCGA , and ( CT ) ( 8 ) alone without the insertion , of which ( CT ) ( 9 ) and the insertion was a new allele identified in only one rice , IR1552 .
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Score: 2.00 |
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| Title: [ Gene expression of the key enzymes controlling starch synthesis and metabolism in rice grain endosperm under effects of high temperature after anthesis ] .
| | Author: Zhong LJ Dong H Cai XB Feng YN Ren P Cheng FM | | Journal: Ying Yong Sheng Tai Xue Bao Citation: V : 23 P : 745-50 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22720620 Accession (PMID): 22720620 | | Abstract: Taking an early-season indica cultivar Jiazao 935 whose grain quality was sensitive to temperature as test material , and by using artificial climatic chamber and real-time fluorescence quantitative PCR ( FQ-PCR ) , this paper studied the relative expression amount and its dynamic changes of ten isoform genes of the key enzymes controlling starch synthesis and metabolism in rice grain endosperm , including sbe1 , sbe3 , and sbe4 of starch branching enzyme ( SBE ) , isal , isa2 , isa3 , and pul of starch debranching enzyme ( DBE ) , and Wx , sss1 , and sss2a of starch synthase ( SS ) , at the mean daily temperature 22 and 32 degrees C after anthesis .
There existed obvious differences in the expression patterns of these genes under the high temperature stress , and the expression patterns were isoform-dependent .
The relative expression amount of sbe1 and sbe3 under high temperature decreased significantly , and both of the genes were the sensitive isoform genes of SBE to high temperature stress .
Among the DBE genes , pul was the isoform gene with high expression level , being more sensitive to high temperature stress than isa1 , isa2 , and isa3 .
Among the SS genes , sss2a had a significantly lower relative expression amount than sss1 and Wx , but sss2a and sss1 were more sensitive to high temperature than Wx , suggesting that sss2a and sss1 could be the important genes that adjusted the starch structure in rice endosperm under high temperature stress , especially at the middle and late grain filling stages .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Taking an early-season indica cultivar Jiazao 935 whose grain quality was sensitive to temperature as test material , and by using artificial climatic chamber and real-time fluorescence quantitative PCR ( FQ-PCR ) , this paper studied the relative expression amount and its dynamic changes of ten isoform genes of the key enzymes controlling starch synthesis and metabolism in rice grain endosperm , including sbe1 , sbe3 , and sbe4 of starch branching enzyme ( SBE ) , isal , isa2 , isa3 , and pul of starch debranching enzyme ( DBE ) , and Wx , sss1 , and sss2a of starch synthase ( SS ) , at the mean daily temperature 22 and 32 degrees C after anthesis .
[ Sen. 3, subscore: 1.00 ]: The relative expression amount of sbe1 and sbe3 under high temperature decreased significantly , and both of the genes were the sensitive isoform genes of SBE to high temperature stress .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
