| 77 matches found in 41 documents. Search time: 0.132 seconds. |
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Score: 9.00 |
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| Title: Heterogeneity of rice ragged stunt oryzavirus genome segment 9 and its segregation by insect vector transmission .
| | Author: Suga H Uyeda I Yan J Murao K Kimura I Tiongco ER Cabautan P Koganezawa H | | Journal: Arch . Virol . Citation: V : 140 ( 8 ) P : 1503-9 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub7661702 Accession (PMID): 7661702 | | Abstract: Genomic heterogeneity of genome segment 9 ( S9 ) of rice ragged stunt virus ( RRSV ) was investigated and a point mutation was found to be responsible for an electrophoretic mobility shift of S9 on polyacrylamide gel electrophoresis ( PAGE ) .
A new form of S9 ( S9L ) which migrated slightly faster than natural S9 ( S9U ) had the same length with A-->C transversion at nt 843 .
Synthetic S9 with a C : G pair at nt 843 migrated slightly faster than that with an A : U pair .
Therefore , we conclude that the single point mutation shifts the electrophoretic mobility .
Using polymerase chain reaction-single strand conformation polymorphism ( PCR-SSCP ) , we could detect S9U and S9L alone or mixture in insect vectors after acquisition as well as in infected rice plants . | Matching Sentences:
[ Sen. 2, subscore: 4.00 ]: A new form of S9 ( S9L ) which migrated slightly faster than natural S9 ( S9U ) had the same length with A-->C transversion at nt 843 .
[ Sen. 1, subscore: 2.00 ]: Genomic heterogeneity of genome segment 9 ( S9 ) of rice ragged stunt virus ( RRSV ) was investigated and a point mutation was found to be responsible for an electrophoretic mobility shift of S9 on polyacrylamide gel electrophoresis ( PAGE ) .
[ Sen. 5, subscore: 2.00 ]: Using polymerase chain reaction-single strand conformation polymorphism ( PCR-SSCP ) , we could detect S9U and S9L alone or mixture in insect vectors after acquisition as well as in infected rice plants .
[ Sen. 3, subscore: 1.00 ]: Synthetic S9 with a C : G pair at nt 843 migrated slightly faster than that with an A : U pair .
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Score: 6.00 |
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| Title: Chemical components , palatability , antioxidant activity and antimutagenicity of oncom miso using a mixture of fermented soybeans and okara with Neurospora intermedia .
| | Author: Matsuo M | | Journal: J Nutr . Sci . Vitaminol . Citation: V : 52 ( 3 ) P : 216-22 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16967767 Accession (PMID): 16967767 | | Abstract: The enzyme activities of Aspergillus oryzae on koji ( malted rice ) and Neurospora intermedia on S-oncom and O-oncom ( fermented soybeans and okara with N intermedia , respectively ) were compared .
The major enzymes of N intermedia were different from those of A oryzae , and the enzyme activities of O-oncom were extremely higher than those of S-oncom .
S5-Miso , S10-miso and S9O1-miso replacing 50% or 100% of steamed soybeans with S-oncom or a 9 : 1 mixture of S-oncom and O-oncom , respectively , were prepared to supplement the enzyme action of koji .
The chemical components of those miso were almost the same as those of soybean-miso ( C-miso ) .
The miso soups prepared with S5-miso , S10-miso and S9O1-miso were all considered to be more palatable and pleasant-tasting than the soup prepared with C-miso , and their flavor was preferred in the same degree as that of T5-miso using 50% tempeh , the soybeans fermented with Rhizopus oligosporus .
Scavenging activities of DPPH and O2 and antimutagenicity of the 70% ethanol extract from those miso were higher than those of hot-water extract , and the activities of S9O1-miso were the highest The isoflavone-aglycons and brownish color of S9O1-miso were higher than those of C-miso .
The higher contents of isoflavone-aglycons and melanoidines of S9O1-miso might contribute to their higher antioxidant activity and antimutagenicity .
From these results , S9O1-miso has potential as a healthier alternative to C-miso in terms of taste and health benefits . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Scavenging activities of DPPH and O2 and antimutagenicity of the 70% ethanol extract from those miso were higher than those of hot-water extract , and the activities of S9O1-miso were the highest The isoflavone-aglycons and brownish color of S9O1-miso were higher than those of C-miso .
[ Sen. 3, subscore: 1.00 ]: S5-Miso , S10-miso and S9O1-miso replacing 50% or 100% of steamed soybeans with S-oncom or a 9 : 1 mixture of S-oncom and O-oncom , respectively , were prepared to supplement the enzyme action of koji .
[ Sen. 5, subscore: 1.00 ]: The miso soups prepared with S5-miso , S10-miso and S9O1-miso were all considered to be more palatable and pleasant-tasting than the soup prepared with C-miso , and their flavor was preferred in the same degree as that of T5-miso using 50% tempeh , the soybeans fermented with Rhizopus oligosporus .
[ Sen. 7, subscore: 1.00 ]: The higher contents of isoflavone-aglycons and melanoidines of S9O1-miso might contribute to their higher antioxidant activity and antimutagenicity .
[ Sen. 8, subscore: 1.00 ]: From these results , S9O1-miso has potential as a healthier alternative to C-miso in terms of taste and health benefits .
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Score: 6.00 |
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| Title: [ Mutagenicity of rice-straw and -husk ashes by Ames test ] | | Author: Shibuya N | | Journal: Citation: V : 38 ( 7 ) P : 507-16 Year: 1991 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1747541 Accession (PMID): 1747541 | | Abstract: In the rice-producing district of Japan , environmental pollution by smoke from burnt rice straws has become a matter of concern .
The mutagenicity of fly and bottom-ashes of rice-straw and -husk was assayed by the Ames test , TA100 +/- S9 and TA98 +/- S9 , and the relationship of combustion temperature to mutagenicity was investigated .
Fly ash showed weak mutagenicity at 300 degrees C , with no remarkable change in mutagenic activity between 300 degrees C and 500 degrees C Above 500 degrees C the mutagenic activity increased with a rise of temperature .
The increasing rate of mutagenicity was much higher in the test system with S9 mix than that without S9 .
Moreover , the mutagenicity with TA100 was stronger than with TA98 .
With respect to bottom ash , weak mutagenic activity was observed at 300 degrees C , but at 400 degrees C decreased and at 500 degrees C or above could not be observed .
Fly ash derived from burning 1 g of rice straw at 600 degrees C showed about 14 times and 2 . 5 times higher mutagenicity than main stream smoke condensates from the burning of 1 g of cigarette in TA100 + S9 and TA98 + S9 test systems respectively . | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: The mutagenicity of fly and bottom-ashes of rice-straw and -husk was assayed by the Ames test , TA100 +/- S9 and TA98 +/- S9 , and the relationship of combustion temperature to mutagenicity was investigated .
[ Sen. 4, subscore: 2.00 ]: The increasing rate of mutagenicity was much higher in the test system with S9 mix than that without S9 .
[ Sen. 7, subscore: 2.00 ]: Fly ash derived from burning 1 g of rice straw at 600 degrees C showed about 14 times and 2 . 5 times higher mutagenicity than main stream smoke condensates from the burning of 1 g of cigarette in TA100 + S9 and TA98 + S9 test systems respectively .
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Score: 5.00 |
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| Title: Cloning and Sequencing of the Genome Segment S9 of Rice Black-streaked Dwarf Virus .
| | Author: Zhang HM Chen JP Cheng Y Lei JL Xue QZ .
| | Journal: Citation: V : 33 ( 4 ) P : 467-471 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12040425 Accession (PMID): 12040425 | | Abstract: Genome segment S9s of rice black-streaked dwarf virus ( RBSDV ) of three Chinese isolates were amplified by RT-PCR and sequenced , and were found to be consisted of 1 900 nt ( RBSDV-Zj S9 EMBL accession number AJ297430 ) , 1 898 nt ( RBSDV-Heb S9 EMBL accession number AJ297429 ) and 1 900 nt ( RBSDV-Hub S9 EMBL accession number AJ291706 ) , respectively .
Genome segment S9s of three Chinese Isolates shared 98 . 5%--98 . 8% sequence homology and all contained two open reading frames ( ORF ) , which encoded two polypeptides with moleclular weights of 40 kD and 24 kD , respectively .
Amino acid sequence comparison of the polypeptides encoded by the second ORF of the corresponding genomic segment of five isolates , including three Chinese isolates , Japanese isolate , and Italian MRDV ( maize rough dwarf virus ) , were highly conserved .
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[ Sen. 1, subscore: 4.00 ]: Genome segment S9s of rice black-streaked dwarf virus ( RBSDV ) of three Chinese isolates were amplified by RT-PCR and sequenced , and were found to be consisted of 1 900 nt ( RBSDV-Zj S9 EMBL accession number AJ297430 ) , 1 898 nt ( RBSDV-Heb S9 EMBL accession number AJ297429 ) and 1 900 nt ( RBSDV-Hub S9 EMBL accession number AJ291706 ) , respectively .
[ Sen. 2, subscore: 1.00 ]: Genome segment S9s of three Chinese Isolates shared 98 . 5%--98 . 8% sequence homology and all contained two open reading frames ( ORF ) , which encoded two polypeptides with moleclular weights of 40 kD and 24 kD , respectively .
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Score: 4.00 |
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| Title: [ Cloning and expression of S9-1 gene of rice black-streaked dwarf virus in Escherichia coli ] | | Author: Li C Zhong Y Zhang X Wei C Li Y | | Journal: Wei Sheng Wu Xue Bao Citation: V : 43 ( 3 ) P : 330-5 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16279198 Accession (PMID): 16279198 | | Abstract: The cDNA of RBSDV S9 was amplified by RT-PCR using the total RNA of infected maize leaves as the template , and then the PCR product was cloned into pBluescriptII SK .
After the DNA sequence was determined , the S9-1 gene was subcloned into E coli expression vector pET-21d .
SDS-PAGE analysis revealed that a 40 kD protein was highly expressed .
N-terminal sequence analysis confirmed the 40 kD protein was S9-1 protein .
Antiserum was prepared using the purified protein .
Western blotting indicated that S9-1 protein could be detected in the infected maize leaves .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The cDNA of RBSDV S9 was amplified by RT-PCR using the total RNA of infected maize leaves as the template , and then the PCR product was cloned into pBluescriptII SK .
[ Sen. 2, subscore: 1.00 ]: After the DNA sequence was determined , the S9-1 gene was subcloned into E coli expression vector pET-21d .
[ Sen. 4, subscore: 1.00 ]: N-terminal sequence analysis confirmed the 40 kD protein was S9-1 protein .
[ Sen. 6, subscore: 1.00 ]: Western blotting indicated that S9-1 protein could be detected in the infected maize leaves .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
