| 73 matches found in 35 documents. Search time: 0.101 seconds. |
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Score: 8.00 |
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| Title: [ Effect of Tanshinone IIA on phosphorylated NMDA receptor 1 expression and intracellular free calcium concentration in neonatal SD rats with hypoxic ischemic brain damage ] .
| | Author: Hei M Liu F Luo Y | | Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban Citation: V : 35 P : 940-6 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20871158 Accession (PMID): 20871158 | | Abstract: OBJECTIVE : To determine the effect of Tanshinone IIA ( TanIIA ) on the phosphory-lated NMDA receptor 1 at Serine 897 site ( phospho-NR1 S897 ) and intracellular free calcium concentration ( [ Ca ( 2+ ) ] ( i ) ) in neonatal SD rats with hypoxic ischemic brain damage ( HIBD ) , and to explore the neuroprotective mechanism of TanIIA in HIBD .
METHODS : Neonatal SD rats were randomly divided into a normal control , and an HIBD and TanIIA+HIBD group .
Rice-Vannucci method was used for HIBD animal model .
Time points were : 3 , 6 , 12 , and 24 h after HIBD ( n=10 in each group at each time point ) .
TanIIA was intraperitoneally given at 1 mug/g every 12 h .
Fura-2AM was used to mark the fluorescent calcium probe and [ Ca ( 2+ ) ] ( i ) was measured by a Hitachi F-4500 Fluorescence Spectrophometer .
Fluorescent immunohisotichemical study was used for the expression of phospho-NR1 S897 .
RESULTS : ( 1 ) Compared with the normal control group , both the [ Ca ( 2+ ) ] ( i ) absolute number and ipsi-/contra-lateral ratio were increased at each time point with statistical significance ( P<0 . 05 ) .
Compared with the HIBD group , the [ Ca ( 2+ ) ] ( i ) in the HIBD+ TanIIA group was decreased at each time point .
At 24 h after HIBD , the ipsi-/contra-lateral ratio of HIBD+ TanIIA group was 24 . 9% less than that of HIBD group with statistical significance ( P<0 . 05 ) .
( 2 ) In the normal control group , abundant phospho-NR1 S897 positive cells were nicely distributed in the cortex Compared with the normal control group , at each time point , both the absolute number of phospho-NR1 S897 positive cells and the fluorescent intensity of phospho-NR1 S897 in the ipsilateral cortex of the HIBD group were decreased with statistical significance ( P<0 . 05 ) .
Compared with the HIBD group , both the absolute number of phospho-NR1 S897 positive cells and the fluorescent intensity of phospho-NR1 S897 in the ipsilateral cortex of HIBD+ TanIIA were increased .
There was significant difference at 3 and 12 h after the HIBD ( P<0 . 05 ) .
CONCLUSION : TanIIA reduced the HIBD-caused down-regulation of phospho-NR1 S897 and the HIBD-caused [ Ca ( 2+ ) ] ( i ) elevation in the cortex The neuroprotective effect of TanIIA may be related to influencing NMDA receptor expression and decreasing intracellular free calcium aggregation .
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[ Sen. 11, subscore: 3.00 ]: ( 2 ) In the normal control group , abundant phospho-NR1 S897 positive cells were nicely distributed in the cortex Compared with the normal control group , at each time point , both the absolute number of phospho-NR1 S897 positive cells and the fluorescent intensity of phospho-NR1 S897 in the ipsilateral cortex of the HIBD group were decreased with statistical significance ( P<0 . 05 ) .
[ Sen. 12, subscore: 2.00 ]: Compared with the HIBD group , both the absolute number of phospho-NR1 S897 positive cells and the fluorescent intensity of phospho-NR1 S897 in the ipsilateral cortex of HIBD+ TanIIA were increased .
[ Sen. 1, subscore: 1.00 ]: OBJECTIVE : To determine the effect of Tanshinone IIA ( TanIIA ) on the phosphory-lated NMDA receptor 1 at Serine 897 site ( phospho-NR1 S897 ) and intracellular free calcium concentration ( [ Ca ( 2+ ) ] ( i ) ) in neonatal SD rats with hypoxic ischemic brain damage ( HIBD ) , and to explore the neuroprotective mechanism of TanIIA in HIBD .
[ Sen. 7, subscore: 1.00 ]: Fluorescent immunohisotichemical study was used for the expression of phospho-NR1 S897 .
[ Sen. 14, subscore: 1.00 ]: CONCLUSION : TanIIA reduced the HIBD-caused down-regulation of phospho-NR1 S897 and the HIBD-caused [ Ca ( 2+ ) ] ( i ) elevation in the cortex The neuroprotective effect of TanIIA may be related to influencing NMDA receptor expression and decreasing intracellular free calcium aggregation .
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Score: 7.00 |
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| Title: Involvement of recently cultured group U2 bacterium in ruminal fiber digestion revealed by coculture with Fibrobacter succinogenes S85 .
| | Author: Fukuma N Koike S Kobayashi Y | | Journal: FEMS Microbiol Lett Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22849722 Accession (PMID): 22849722 | | Abstract: In a previous study , we reported the ecological significance of uncultured bacterial group U2 in the rumen .
In this study , the involvement of a recently-cultured group U2 bacterium , strain R-25 , in fiber digestion was tested in coculture with the fibrolytic bacterium Fibrobacter succinogenes S85 .
Dry matter ( DM ) digestion , growth and metabolites were examined in culture using rice straw as the carbon source .
Although strain R-25 did not digest rice straw in monoculture , coculture of strain R-25 and F succinogenes S85 showed enhanced DM digestion compared to that for F succinogenes S85 monoculture ( 36 . 9+/-0 . 6% vs 32 . 8+/-1 . 3% , P < 0 . 05 ) .
Growth of strain R-25 and production of the main metabolites , D-lactate ( strain R-25 ) and succinate ( F succinogenes S85 ) , were enhanced in the coculture .
Enzyme assay showed increased activities of carboxymethylcellulase and xylanase in coculture of strain R-25 and F succinogenes S85 .
Triculture including strain R-25 , F succinogenes S85 and Selenomonas ruminantium S137 showed a further increase in DM digestion ( 41 . 8+/-0 . 8% , P < 0 . 05 ) with a concomitant increase of propionate , produced from the conversion of D-lactate and succinate .
These results suggest that the positive interaction between strains R-25 and F succinogenes S85 causes increased rice straw digestion .
( c ) 2012 Federation of European Microbiological Societies .
Published by Blackwell Publishing Ltd .
All rights reserved .
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[ Sen. 4, subscore: 2.00 ]: Although strain R-25 did not digest rice straw in monoculture , coculture of strain R-25 and F succinogenes S85 showed enhanced DM digestion compared to that for F succinogenes S85 monoculture ( 36 . 9+/-0 . 6% vs 32 . 8+/-1 . 3% , P < 0 . 05 ) .
[ Sen. 2, subscore: 1.00 ]: In this study , the involvement of a recently-cultured group U2 bacterium , strain R-25 , in fiber digestion was tested in coculture with the fibrolytic bacterium Fibrobacter succinogenes S85 .
[ Sen. 5, subscore: 1.00 ]: Growth of strain R-25 and production of the main metabolites , D-lactate ( strain R-25 ) and succinate ( F succinogenes S85 ) , were enhanced in the coculture .
[ Sen. 6, subscore: 1.00 ]: Enzyme assay showed increased activities of carboxymethylcellulase and xylanase in coculture of strain R-25 and F succinogenes S85 .
[ Sen. 7, subscore: 1.00 ]: Triculture including strain R-25 , F succinogenes S85 and Selenomonas ruminantium S137 showed a further increase in DM digestion ( 41 . 8+/-0 . 8% , P < 0 . 05 ) with a concomitant increase of propionate , produced from the conversion of D-lactate and succinate .
[ Sen. 8, subscore: 1.00 ]: These results suggest that the positive interaction between strains R-25 and F succinogenes S85 causes increased rice straw digestion .
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Score: 7.00 |
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| Title: The M ( r ) 43K major capsid protein of rice ragged stunt oryzavirus is a post-translationally processed product of a M ( r ) 67 , 348 polypeptide encoded by genome segment 8 .
| | Author: Upadhyaya NM Zinkowsky E Kositratana W Waterhouse PM .
| | Journal: Arch . Virol . Citation: V : 141 ( 9 ) P : 1689-701 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8893791 Accession (PMID): 8893791 | | Abstract: The nucleotide sequence of DNA complementary to rice ragged stunt oryzavirus ( RRSV ) genome segment 8 ( S8 ) of an isolate from Thailand was determined .
RRSVS8 is 1914 bp in size and contains a single large open reading frame ( ORF ) spanning nucleotides 23 to 1810 which is capable of encoding a protein of M ( r ) 67 , 348 .
The N-terminal amino acid sequence of a approximately 43K virion polypeptide matched to that inferred for an internal region of the S8 coding sequence .
These data suggest that the 43K protein is encoded by S8 and is derived by a proteolytic cleavage .
Predicted polypeptide sizes from this possible cleavage of S8 protein are 26K and 42K .
Polyclonal antibodies raised against a maltose binding protein ( MBP ) -S8 fusion polypeptide ( expressed in Escherichia coli ) recognised four RRSV particle associated polypeptides of M ( r ) 67K , 46K , 43K and 26K and all except the 26K polypeptide were also highly immunoreactive to polyclonal antibodies raised against purified RRSV particles .
Cleavage of the MBP-S8 fusion polypeptide with protease Factor X produced the expected 40K MBP and two polypeptides of apparent M ( r ) 46K and 26K .
Antibodies to purified RRSV particles reacted strongly with the intact fusion protein and the 46K cleavage product but weakly to the 26K product .
Furthermore , in vitro transcription and translation of the S8 coding region revealed a post-translational self cleavage of the 67K polypeptide to 46K and 26K products .
These data indicate that S8 encodes a structural polypeptide , the majority of which is auto-catalytically cleaved to 26K and 46K proteins .
The data also suggest that the 26K protein is the self cleaving protease and that the 46K product is further processed or undergoes stable conformational changes to a approximately 43K major capsid protein . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The nucleotide sequence of DNA complementary to rice ragged stunt oryzavirus ( RRSV ) genome segment 8 ( S8 ) of an isolate from Thailand was determined .
[ Sen. 3, subscore: 1.00 ]: The N-terminal amino acid sequence of a approximately 43K virion polypeptide matched to that inferred for an internal region of the S8 coding sequence .
[ Sen. 4, subscore: 1.00 ]: These data suggest that the 43K protein is encoded by S8 and is derived by a proteolytic cleavage .
[ Sen. 5, subscore: 1.00 ]: Predicted polypeptide sizes from this possible cleavage of S8 protein are 26K and 42K .
[ Sen. 6, subscore: 1.00 ]: Polyclonal antibodies raised against a maltose binding protein ( MBP ) -S8 fusion polypeptide ( expressed in Escherichia coli ) recognised four RRSV particle associated polypeptides of M ( r ) 67K , 46K , 43K and 26K and all except the 26K polypeptide were also highly immunoreactive to polyclonal antibodies raised against purified RRSV particles .
[ Sen. 9, subscore: 1.00 ]: Furthermore , in vitro transcription and translation of the S8 coding region revealed a post-translational self cleavage of the 67K polypeptide to 46K and 26K products .
[ Sen. 10, subscore: 1.00 ]: These data indicate that S8 encodes a structural polypeptide , the majority of which is auto-catalytically cleaved to 26K and 46K proteins .
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Score: 5.00 |
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| Title: Nucleotide sequences of genome segments S8 , encoding a capsid protein , and S10 , encoding a 36K protein , of rice gall dwarf virus .
| | Author: Noda H Ishikawa K Hibino H Kato H Omura T | | Journal: J Gen . Virol . Citation: V : 72 ( Pt 11 ) ( ) P : 2837-42 Year: 1991 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1940872 Accession (PMID): 1940872 | | Abstract: The nucleotide sequences of DNAs complementary to the eighth ( S8 ) and the tenth ( S10 ) largest of the 12 genome segments of rice gall dwarf virus ( RGDV ) were determined .
The S8 and S10 segments consist of 1578 and 1198 nucleotides , each with a single open reading frame extending for 1278 nucleotides from nucleotide 21 , and 960 nucleotides from nucleotide 22 , respectively .
S8 encodes a polypeptide of 426 amino acids with an Mr of 47419 .
The amino acid sequences of several peptide fragments of the major outer capsid protein reported as 45K were contained in the predicted polypeptide .
This protein , renamed the 47K protein , showed high homology with the outer capsid proteins of rice dwarf virus ( RDV ) and wound tumour virus ( WTV ) ; there was 56 , 52 and 48% amino acid sequence identity between RGDV and WTV , RGDV and RDV , and RDV and WTV , respectively .
S10 had the coding potential for a polypeptide of 320 amino acids with an Mr of 36 , 095 ( 36K protein ) , which exhibits 32% and 35% amino acid sequence identity with the predicted translation product of RDV S9 and the P9 capsid protein encoded by WTV S11 , respectively .
The conserved terminal sequences 5 GG . . . GAU 3 which are present in all genome segments of WTV and RDV so far analysed , and in S9 of RGDV , were also found in RGDV S8 and S10 .
This conserved sequence together with the segment-specific inverted repeats found in the terminal sequence of RGDV S8 and S10 are thus characteristic structures common to all three phytoreoviruses .
The nucleotide sequence of the region surrounding the inverted repeats was more similar between RGDV and WTV than between RGDV and RDV . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The nucleotide sequences of DNAs complementary to the eighth ( S8 ) and the tenth ( S10 ) largest of the 12 genome segments of rice gall dwarf virus ( RGDV ) were determined .
[ Sen. 2, subscore: 1.00 ]: The S8 and S10 segments consist of 1578 and 1198 nucleotides , each with a single open reading frame extending for 1278 nucleotides from nucleotide 21 , and 960 nucleotides from nucleotide 22 , respectively .
[ Sen. 3, subscore: 1.00 ]: S8 encodes a polypeptide of 426 amino acids with an Mr of 47419 .
[ Sen. 7, subscore: 1.00 ]: The conserved terminal sequences 5 GG . . . GAU 3 which are present in all genome segments of WTV and RDV so far analysed , and in S9 of RGDV , were also found in RGDV S8 and S10 .
[ Sen. 8, subscore: 1.00 ]: This conserved sequence together with the segment-specific inverted repeats found in the terminal sequence of RGDV S8 and S10 are thus characteristic structures common to all three phytoreoviruses .
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Score: 4.00 |
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| Title: Sequence analysis of genome segments S4 and S8 of Mal de R Cuarto virus ( MRCV ) : evidence that the virus should be a separate Fijivirus species .
| | Author: Distfano AJ Conci LR Muoz Hidalgo M Guzmn FA Hopp HE del Vas M | | Journal: Arch . Virol . Citation: V : 147 ( 9 ) P : 1699-709 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12209310 Accession (PMID): 12209310 | | Abstract: This is the first sequence-based characterization of Mal de R Cuarto virus ( MRCV ) , currently classified as a variant of Maize rough dwarf virus ( MRDV ) and exclusively found in South America .
We sequenced and analyzed genome segments S4 and S8 .
MRCV S4 coded for a putative 131 . 67 kDa protein while MRCV S8 coded for a putative 68 . 26 kDa protein containing an ATP/GTP-binding motif .
The 5 and 3 ends of MRCV segments , were 5AAGUUUUU3 and 5CAGCUnnnGUC3 , respectively .
Prediction of secondary structure of both segments coding strands showed that terminal regions were able to form structures that are proposed to be replication and packaging signals .
MRCV S4 showed identity to members of Fijivirus as well as to two other genera of the Reoviridae family .
MRCV S8 revealed identity with Rice black streaked dwarf virus ( RBSDV ) S8 , MRDV S7 , Oat sterile dwarf virus ( OSDV ) S9 and Nilaparvata lugens reovirus ( NLRV ) S7 .
While MRDV and RBSDV segments are highly homologous between each other , MRCV identity levels with them was considerably lower .
We discussed the evolutionary relationships of MRCV to other Reoviridae , and based on phylogenetic analysis we proposed that although MRCV is related to MRDV , it could be regarded as a new species of the Fijivirus genus .
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[ Sen. 7, subscore: 2.00 ]: MRCV S8 revealed identity with Rice black streaked dwarf virus ( RBSDV ) S8 , MRDV S7 , Oat sterile dwarf virus ( OSDV ) S9 and Nilaparvata lugens reovirus ( NLRV ) S7 .
[ Sen. 2, subscore: 1.00 ]: We sequenced and analyzed genome segments S4 and S8 .
[ Sen. 3, subscore: 1.00 ]: MRCV S4 coded for a putative 131 . 67 kDa protein while MRCV S8 coded for a putative 68 . 26 kDa protein containing an ATP/GTP-binding motif .
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