Score: 8.00 |
|---|
| Title: Differential , phosphorylation dependent trafficking of AQP2 in LLC-PK1 cells .
| | Author: Rice WL Zhang Y Chen Y Matsuzaki T Brown D Lu HA | | Journal: PLoS One Citation: V : 7 P : e32843 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22403603 Accession (PMID): 22403603 | | Abstract: The kidney maintains water homeostasis by modulating aquaporin 2 ( AQP2 ) on the plasma membrane of collecting duct principal cells in response to vasopressin ( VP ) .
VP mediated phosphorylation of AQP2 at serine 256 is critical for this effect .
However , the role of phosphorylation of other serine residues in the AQP2 C-terminus is less well understood .
Here , we examined the effect of phosphorylation of S256 , S261 and S269 on AQP2 trafficking and association with recycling pathway markers .
We used LLC-PK1 cells expressing AQP2 ( S-D ) or ( S-A ) phospho mutants and a 20 degrees C cold block , which allows endocytosis to continue , but prevents protein exit from the trans Golgi network ( TGN ) , inducing formation of a perinuclear AQP2 patch .
AQP2-S256D persists on the plasma membrane during cold block , while wild type AQP2 , AQP2-S256A , S261A , S269A and S269D are internalized and accumulate in the patch .
Development of this patch , a measure of AQP2 internalization , was most rapid with AQP2-S256A , and slowest with S261A and S269D .
AQP2-S269D exhibited a biphasic internalization profile with a significant amount not internalized until 150 minutes of cold block .
After rewarming to 37 degrees C , wt AQP2 , AQP2-S261A and AQP2-S269D rapidly redistributed throughout the cytoplasm within 20 minutes , whereas AQP2-S256A dissipated more slowly .
Colocalization of AQP2 mutants with several key vesicular markers including clathrin , HSP70/HSC70 , EEA , GM130 and Rab11 revealed no major differences .
Overall , our data provide evidence supporting the role of S256 and S269 in the maintenance of AQP2 at the cell surface and reveal the dynamics of internalization and recycling of differentially phosphorylated AQP2 in cell culture .
| Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: AQP2-S256D persists on the plasma membrane during cold block , while wild type AQP2 , AQP2-S256A , S261A , S269A and S269D are internalized and accumulate in the patch .
[ Sen. 4, subscore: 2.00 ]: Here , we examined the effect of phosphorylation of S256 , S261 and S269 on AQP2 trafficking and association with recycling pathway markers .
[ Sen. 7, subscore: 2.00 ]: Development of this patch , a measure of AQP2 internalization , was most rapid with AQP2-S256A , and slowest with S261A and S269D .
[ Sen. 11, subscore: 1.00 ]: Overall , our data provide evidence supporting the role of S256 and S269 in the maintenance of AQP2 at the cell surface and reveal the dynamics of internalization and recycling of differentially phosphorylated AQP2 in cell culture .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Map-based cloning proves qGC-6 , a major QTL for gel consistency of japonica/indica cross , responds by Waxy in rice ( Oryza sativa L ) .
| | Author: Su Y Rao Y Hu S Yang Y Gao Z Zhang G Liu J Hu J Yan M Dong G Zhu L Guo L Qian Q Zeng D | | Journal: Theor Appl Genet Citation: V : 123 P : 859-67 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21698394 Accession (PMID): 21698394 | | Abstract: In this study , one major QTL affecting gel consistency ( GC ) of japonica/indica cross was identified on chromosome 6 using a DH population .
To understand the molecular mechanism that regulates GC in rice grains , the major QTL ( qGC-6 ) was isolated through a map-based cloning approach utilizing chromosome segment substitution lines ( CSSLs ) .
Using 64 plants with extremely soft GC that were selected on recombinant break points between two SSR markers , RM540 and RM8200 in a BC4F2 population , qGC-6 was mapped to a 60-kb DNA region between two STS markers , S26 and S27 .
These two markers were then used to further identify recombination break points .
Finally , qGC-6 was delimited in an interval of a 11-kb region .
Gene prediction analysis of the 11-kb DNA sequence containing qGC-6 identified only one putative ORF , which encodes granule-bound starch synthesis protein ( Wx protein ) .
Results of sequencing analysis and complementation experiment confirmed that this candidate ORF is responsible for rice GC .
Genetic evidences revealed that Wx might contribute equally to the grain amylose content-controlling gene as well as gel consistency .
This new information is important to breed rice varieties with improved grain quality .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Using 64 plants with extremely soft GC that were selected on recombinant break points between two SSR markers , RM540 and RM8200 in a BC4F2 population , qGC-6 was mapped to a 60-kb DNA region between two STS markers , S26 and S27 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
