| 13 matches found in 2 documents. Search time: 0.041 seconds. |
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Score: 10.00 |
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| Title: Hybrid male sterility in rice is due to epistatic interactions with a pollen killer locus .
| | Author: Kubo T Yoshimura A Kurata N | | Journal: Genetics Citation: V : 189 P : 1083-92 Year: 2011 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub21868603 Accession (PMID): 21868603 | | Abstract: In intraspecific crosses between cultivated rice ( Oryza sativa ) subspecies indica and japonica , the hybrid male sterility gene S24 causes the selective abortion of male gametes carrying the japonica allele ( S24-j ) via an allelic interaction in the heterozygous hybrids .
In this study , we first examined whether male sterility is due solely to the single locus S24 .
An analysis of near-isogenic lines ( NIL-F ( 1 ) ) showed different phenotypes for S24 in different genetic backgrounds .
The S24 heterozygote with the japonica genetic background showed male semisterility , but no sterility was found in heterozygotes with the indica background .
This result indicates that S24 is regulated epistatically .
A QTL analysis of a BC ( 2 ) F ( 1 ) population revealed a novel sterility locus that interacts with S24 and is found on rice chromosome 2 .
The locus was named Epistatic Factor for S24 ( EFS ) .
Further genetic analyses revealed that S24 causes male sterility when in combination with the homozygous japonica EFS allele ( efs-j ) .
The results suggest that efs-j is a recessive sporophytic allele , while the indica allele ( EFS-i ) can dominantly counteract the pollen sterility caused by S24 heterozygosity .
In summary , our results demonstrate that an additional epistatic locus is an essential element in the hybrid sterility caused by allelic interaction at a single locus in rice .
This finding provides a significant contribution to our understanding of the complex molecular mechanisms underlying hybrid sterility and microsporogenesis .
| Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: In intraspecific crosses between cultivated rice ( Oryza sativa ) subspecies indica and japonica , the hybrid male sterility gene S24 causes the selective abortion of male gametes carrying the japonica allele ( S24-j ) via an allelic interaction in the heterozygous hybrids .
[ Sen. 2, subscore: 1.00 ]: In this study , we first examined whether male sterility is due solely to the single locus S24 .
[ Sen. 3, subscore: 1.00 ]: An analysis of near-isogenic lines ( NIL-F ( 1 ) ) showed different phenotypes for S24 in different genetic backgrounds .
[ Sen. 4, subscore: 1.00 ]: The S24 heterozygote with the japonica genetic background showed male semisterility , but no sterility was found in heterozygotes with the indica background .
[ Sen. 5, subscore: 1.00 ]: This result indicates that S24 is regulated epistatically .
[ Sen. 6, subscore: 1.00 ]: A QTL analysis of a BC ( 2 ) F ( 1 ) population revealed a novel sterility locus that interacts with S24 and is found on rice chromosome 2 .
[ Sen. 7, subscore: 1.00 ]: The locus was named Epistatic Factor for S24 ( EFS ) .
[ Sen. 8, subscore: 1.00 ]: Further genetic analyses revealed that S24 causes male sterility when in combination with the homozygous japonica EFS allele ( efs-j ) .
[ Sen. 9, subscore: 1.00 ]: The results suggest that efs-j is a recessive sporophytic allele , while the indica allele ( EFS-i ) can dominantly counteract the pollen sterility caused by S24 heterozygosity .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
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| Title: Molecular analysis of an additional case of hybrid sterility in rice ( Oryza sativa L ) .
| | Author: Zhao ZG Zhu SS Zhang YH Bian XF Wang Y Jiang L Liu X Chen LM Liu SJ Zhang WW Ikehashi H Wan JM | | Journal: Planta Citation: V : 233 P : 485-94 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21082325 Accession (PMID): 21082325 | | Abstract: Hybrid sterility hinders the exploitation of the heterosis displayed by japonica x indica rice hybrids .
The variation in pollen semi-sterility observed among hybrids between the japonica recipient cultivar and each of two sets of chromosome segment substitution lines involving introgression from an indica cultivar was due to a factor on chromosome 5 known to harbor the gene S24 .
S24 was fine mapped to a 42 kb segment by analyzing a large F ( 2 ) population bred from the cross S24-NIL x Asominori , while the semi-sterility shown by the F ( 1 ) hybrid was ascribable to mitotic failure at the early bicellular pollen stage .
Interestingly , two other pollen sterility genes ( f5-Du and Sb ) map to the same region ( Li et al in Chin Sci Bull 51 : 675-680 , 2006 ; Wang et al in Theor Appl Genet 112 : 382-387 , 2006 ) , allowing a search for candidate genes in the 6 . 4 kb overlap between the three genes .
By sequencing the overlapped fragment in wild rice , indica cultivars and japonica cultivars , a protein ankyrin-3 encoded by the ORF2 was identified as the molecular base for S24 .
A cultivar Dular was found to have a hybrid-sterility-neutral allele , S24-n , in which an insertion of 30 bp was confirmed .
Thus , it was possible to add one more case of molecular bases for the hybrid sterility .
No gamete abortion is caused on heterozygous maternal genotype with an impaired sequence from the hybrid-sterility-neutral genotype .
This result will be useful in understanding of wide compatibility in rice breeding .
| Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: S24 was fine mapped to a 42 kb segment by analyzing a large F ( 2 ) population bred from the cross S24-NIL x Asominori , while the semi-sterility shown by the F ( 1 ) hybrid was ascribable to mitotic failure at the early bicellular pollen stage .
[ Sen. 2, subscore: 1.00 ]: The variation in pollen semi-sterility observed among hybrids between the japonica recipient cultivar and each of two sets of chromosome segment substitution lines involving introgression from an indica cultivar was due to a factor on chromosome 5 known to harbor the gene S24 .
[ Sen. 5, subscore: 1.00 ]: By sequencing the overlapped fragment in wild rice , indica cultivars and japonica cultivars , a protein ankyrin-3 encoded by the ORF2 was identified as the molecular base for S24 .
[ Sen. 6, subscore: 1.00 ]: A cultivar Dular was found to have a hybrid-sterility-neutral allele , S24-n , in which an insertion of 30 bp was confirmed .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
