About Textpresso Categories/Ontology Copyright Downloads Feedback Home Query Language Search User Guide
Enter keyword(s) and/or category/ies. Selecting categories for a query makes a search more specific. For example, you can retrieve sentences that contain the word HSN and a Oryza sativa gene name by typing the keyword 'SPW1' and choosing the category 'gene (Oryza sativa)'. A category hit occurs when a particular word or phrase in the sentence is defined as a member of a particular category. Categories will be concatenated by a Boolean 'and' operation to other categories and keyword(s) if present. To search for terms in categories, click on the Categories/Ontology link above.
Keywords
Separate multiple, required keywords by white spaces (Boolean 'and').
Separate multiple, alternative keywords by a comma with no white spaces (Boolean 'or').
Enter phrases in double quotes, and put a '-' sign in front of words which are to be excluded.
Keyword Specification
AND/OR
Categories
Fields
Search Scope
Search Mode
Sort by
 
Narrow your search results with filter:
Put a '+' sign in front of words which have to be included, a '-' sign in front of words which have to be excluded. Enter the field of the word, viz author, title, year, journal, abstract, type or sentence in square brackets. Enter phrases in double quotes.
For example, to find all the papers in the search result that have Jack as author, but not John, enter +Jack-John[author]. To exclude all papers that have the phrase double mutant in title, enter -"double mutant"[title]. You can combine several filters and enter something like +Jack-John[author] -"double mutant"[title] +1994[year] -review[type].
Click on Filter! button to activate the filter.

Goto:
of 1
Display options:
author: on | off accession: on | off type: on | off abstract: on | off title: on | off
citation: on | off journal: on | off year: on | off supplementals: on | off textlinks: on | off
searchterm-highlighting: on | off matching sentences: none 1 5 10 entries/page: 5 10 20 50
3 matches found in 3 documents. Search time: 0.4 seconds.
Global links/files: all results in endnote all results in print version
Score: 2.00
Title: Identification of amino acids important for substrate specificity in sucrose transporters using gene shuffling .
Author: Reinders A Sun Y Karvonen KL Ward JM
Journal: J Biol Chem Citation: V : P : Year: 2012 Type: Publisher
Literature: oryza Field: abstract Doc ID: pub22807445 Accession (PMID): 22807445
Abstract: Plant sucrose transporters ( SUTs ) are H+-coupled uptake transporters . Type I and II ( SUTs ) are phylogenetically related but have different substrate specificities . Type I SUTs transport sucrose , maltose and a wide range of natural and synthetic alpha and beta-glucosides . Type II SUTs are more selective for sucrose and maltose . Here , we investigated the structural basis for this difference in substrate specificity . We used a novel gene shuffling method called Synthetic Template Shuffling ( STS ) to introduce 62 differentially conserved amino acid residues from type I SUTs into OsSUT1 , a type II SUT from rice . The OsSUT1 variants were tested for their ability to transport the fluorescent coumarin beta-glucoside esculin when expressed in yeast Fluorescent yeast cells were selected using fluorescence-activated cell sorting ( FACS ) . Substitution of five amino acids present in type I SUTs in OsSUT1 was found to be sufficient to confer esculin uptake activity . The changes clustered in two areas of the OsSUT1 protein , in the first loop and the top of TMS2 ( T80L , A86K ) and in TMS5 ( S220A , S221A , T224Y ) . The substrate specificity of this OsSUT1 variant was almost identical to that of type I SUTs . Corresponding changes in the sugarcane type II transporter ShSUT1 also changed substrate specificity indicating that these residues contribute to substrate specificity in type II SUTs in general
Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: The changes clustered in two areas of the OsSUT1 protein , in the first loop and the top of TMS2 ( T80L , A86K ) and in TMS5 ( S220A , S221A , T224Y ) .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: Efficient replication of genotype 3a and 4a HCV replicons in human hepatoma cells .
Author: Saeed M Scheel TK Gottwein JM Marukian S Dustin LB Bukh J Rice CM
Journal: Antimicrob Agents Chemother Citation: V : P : Year: 2012 Type: Publisher
Literature: oryza Field: abstract Doc ID: pub22869572 Accession (PMID): 22869572
Abstract: Despite recent advances in the treatment of hepatitis C , the quest for pan-genotype , effective , and well-tolerated inhibitors continues . To facilitate these efforts , it is desirable to have in vitro replication systems for all major HCV genotypes . However , cell culture replication systems exist for only genotypes 1a , 1b and 2a . In this study , we generated G418-selectable subgenomic replicons for prototype strains of genotype 3a ( S52 ) and 4a ( ED43 ) . Production of G418-resistant colonies by S52 and ED43 in Huh-7 . 5 cells required amino acid substitutions S2210I and R2882G respectively ; cell culture adaptive mutations originally reported for genotype 1b replicons . RNA replication was confirmed by quantitative RT-PCR and detection of viral protein . Sequencing of multiple independent replicon clones revealed the presence of additional non-synonymous mutations . Interestingly , all potentially adaptive mutations mapped to the NS3 protein . These mutations , when introduced back into original constructs , substantially increased colony formation efficiency . To make these replicons useful for high-throughput screening and evaluation of antiviral compounds , they were modified to express a chimeric fusion protein of firefly luciferase and neomycin phosphotransferase to yield stable replicon-expressing cells . Using these constructs , the inhibitory effects of IFNbeta , an NS3 protease inhibitor and an NS5B nucleoside polymerase inhibitor were readily detected by monitoring luciferase activity . In conclusion , we have established functional replicons for HCV genotypes 3a and 4a , important new additions to the armamentarium required to develop inhibitors with a pan-genotype activity .
Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Production of G418-resistant colonies by S52 and ED43 in Huh-7 . 5 cells required amino acid substitutions S2210I and R2882G respectively ; cell culture adaptive mutations originally reported for genotype 1b replicons .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: The primary structure of two proteins from the small ribosomal subunit of rice .
Author: Nishi R Hashimoto H Uchimiya H Kato A
Journal: Biochim . Biophys . Acta Citation: V : 1216 ( 1 ) P : 113-4 Year: 1993 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub8218399 Accession (PMID): 8218399
Abstract: We isolated two rice cDNAs which encode an open reading frame of 117 amino acids or 82 amino acids . Their deduced amino acid sequence correspond to the ribosomal proteins ( r-protein ) . A comparison of the amino acid sequence shows that the deduced amino acid sequence of one cDNA is homologous to the rat r-protein S20 and Xenopus r-protein S22 . Another encoded products with a high degree of homology to the rat r-protein S21 and the yeast r-protein YS25 .
Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: A comparison of the amino acid sequence shows that the deduced amino acid sequence of one cDNA is homologous to the rat r-protein S20 and Xenopus r-protein S22 .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Goto:

Textpresso Wed Jul 17 12:27:13 2019 .