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29 matches found in 14 documents. Search time: 0.128 seconds.
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Score: 6.00
Title: Overrepresentation of the proarrhythmic , sudden death predisposing sodium channel polymorphism S1103Y in a population-based cohort of African-American sudden infant death syndrome .
Author: Van Norstrand DW Tester DJ Ackerman MJ
Journal: Heart Rhythm Citation: V : 5 P : 712-5 Year: 2008 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub18452875 Accession (PMID): 18452875
Abstract: BACKGROUND : The S1103Y-SCN5A polymorphism has been implicated as a proarrhythmic , sudden death predisposing risk factor in African Americans , including one postmortem investigation of African-American infants with sudden infant death syndrome ( SIDS ) . OBJECTIVE : The purpose of this study was to assess whether the relatively African-American-specific common polymorphism S1103Y in the SCN5A-encoded cardiac sodium channel is overrepresented in SIDS among African Americans . METHODS : Seventy-one cases from a population-based cohort of unexplained infant deaths among African Americans ( 37 females and 34 males , average age 3 +/- 2 months , age range birth to 11 months ) were submitted to the Mayo Clinic Windland Smith Rice Sudden Death Genomics Laboratory for postmortem genetic testing . Polymerase chain reaction and a restriction digest assay were performed to genotype this cohort for S1103Y . RESULTS : Targeted mutational analysis of exon 18 in SCN5A of the African-American SIDS cohort ( n = 71 ) revealed the S1103Y polymorphism in 16 ( 22 . 5% ) of 71 African-American cases of SIDS compared to 135 ( 11 . 6% ) of 1 , 161 ostensibly healthy adult African Americans ( P = . 01 ) . CONCLUSION : This study provides an independent assessment of the prevalence of S1103Y-SCN5A among African-American infants with sudden , unexpected , unexplained death prior to their first birthday . Further scrutiny and quantification of the risk apparently associated with S1103Y appear warranted .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: BACKGROUND : The S1103Y-SCN5A polymorphism has been implicated as a proarrhythmic , sudden death predisposing risk factor in African Americans , including one postmortem investigation of African-American infants with sudden infant death syndrome ( SIDS ) .
[ Sen. 2, subscore: 1.00 ]: OBJECTIVE : The purpose of this study was to assess whether the relatively African-American-specific common polymorphism S1103Y in the SCN5A-encoded cardiac sodium channel is overrepresented in SIDS among African Americans .
[ Sen. 4, subscore: 1.00 ]: Polymerase chain reaction and a restriction digest assay were performed to genotype this cohort for S1103Y .
[ Sen. 5, subscore: 1.00 ]: RESULTS : Targeted mutational analysis of exon 18 in SCN5A of the African-American SIDS cohort ( n = 71 ) revealed the S1103Y polymorphism in 16 ( 22 . 5% ) of 71 African-American cases of SIDS compared to 135 ( 11 . 6% ) of 1 , 161 ostensibly healthy adult African Americans ( P = . 01 ) .
[ Sen. 6, subscore: 1.00 ]: CONCLUSION : This study provides an independent assessment of the prevalence of S1103Y-SCN5A among African-American infants with sudden , unexpected , unexplained death prior to their first birthday .
[ Sen. 7, subscore: 1.00 ]: Further scrutiny and quantification of the risk apparently associated with S1103Y appear warranted .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 5.00
Title: Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12 .
Author: Suzuki N Harada M Kusano T
Journal: J Gen . Virol . Citation: V : 72 ( Pt 9 ) ( ) P : 2233-7 Year: 1991 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub1840611 Accession (PMID): 1840611
Abstract: The complete nucleotide sequence of rice dwarf phytoreovirus ( RDV ) genome segment S11 was determined . S11 is 1067 nucleotides long . There is an inverted repeat of 10 bp adjacent to the conserved 5-terminal hexanucleotide ( 5 GGUAAA 3 ) and 3-terminal tetranucleotide ( 5 UAGU 3 ) sequences . A single large open reading frame found in the plus strand of S11 begins with the first AUG codon ( bases 6 to 8 ) and extends for 567 bases . Evolutionary relatedness between RDV S11 and wound tumour phytoreovirus S12 based on amino acid sequence similarity ( 25 . 8% ) was found . In addition to the first AUG triplet , RDV S11 possesses a second in-phase AUG triplet ( positions 30 to 32 ) nearby , which conforms to the Kozak consensus sequence . Two forms of the protein were identified by using an in vitro transcription and translation system in which a tailored full-length cDNA was the initial template . The abolition of the first AUG codon by site-directed mutagenesis resulted in disappearance of the larger translation product . These results strongly suggest that the two products are translated from the first and second AUG codons . Whether the two proteins are expressed in vivo is at present unclear .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The complete nucleotide sequence of rice dwarf phytoreovirus ( RDV ) genome segment S11 was determined .
[ Sen. 2, subscore: 1.00 ]: S11 is 1067 nucleotides long .
[ Sen. 4, subscore: 1.00 ]: A single large open reading frame found in the plus strand of S11 begins with the first AUG codon ( bases 6 to 8 ) and extends for 567 bases .
[ Sen. 5, subscore: 1.00 ]: Evolutionary relatedness between RDV S11 and wound tumour phytoreovirus S12 based on amino acid sequence similarity ( 25 . 8% ) was found .
[ Sen. 6, subscore: 1.00 ]: In addition to the first AUG triplet , RDV S11 possesses a second in-phase AUG triplet ( positions 30 to 32 ) nearby , which conforms to the Kozak consensus sequence .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 3.00
Title: Sequence analysis of Pns11 , a nonstructural protein of rice gall dwarf virus , and its expression and detection in infected rice plants and vector insects .
Author: Moriyasu Y Ishikawa K Kikuchi A Imanishi S Tomita S Akutsu K Omura T
Journal: Virus Genes Citation: V : 20 ( 3 ) P : 237-41 Year: Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub10949951 Accession (PMID): 10949951
Abstract: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined . The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses , which are other members of Phytoreovirus . A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA . An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects . However , the antiserum did not react with purified viral proteins . These results suggest that S11 encodes a nonstructural protein of RGDV . This protein was named Pns11 .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined .
[ Sen. 3, subscore: 1.00 ]: A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA .
[ Sen. 6, subscore: 1.00 ]: These results suggest that S11 encodes a nonstructural protein of RGDV .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 3.00
Title: Targeting presequence acquisition after mitochondrial gene transfer to the nucleus occurs by duplication of existing targeting signals .
Author: Kadowaki K Kubo N Ozawa K Hirai A
Journal: EMBO J Citation: V : 15 ( 23 ) P : 6652-61 Year: 1996 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub8978691 Accession (PMID): 8978691
Abstract: We have cloned a gene for mitochondrial ribosomal protein S11 ( RPS11 ) , which is encoded in lower plants by the mitochondrial genome , in higher plants by the nuclear genome , demonstrating genetic information transfer from the mitochondrial genome to the nucleus during flowering plant evolution . The sequence s11-1 encodes an N-terminal extension as well as an organelle-derived RPS11 region . Surprisingly , the N-terminal region has high amino acid sequence similarity with the presequence of the beta-subunit of ATP synthase from plant mitochondria , suggesting a common lineage of the presequences . The deduced N-terminal region of s11-2 , a second nuclear-encoded homolog of rps11 , shows high sequence similarity with the putative presequence of cytochrome oxidase subunit Vb . The sharing of the N-terminal region together with its 5 flanking untranslated nucleotide sequence in different proteins strongly suggests an involvement of duplication/recombination for targeting signal acquisition after gene migration . A remnant of ancestral rps11 sequence , transcribed and subjected to RNA editing , is found in the mitochondrial genome , indicating that inactivation of mitochondrial rps11 gene expression was initiated at the translational level prior to termination of transcription .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: We have cloned a gene for mitochondrial ribosomal protein S11 ( RPS11 ) , which is encoded in lower plants by the mitochondrial genome , in higher plants by the nuclear genome , demonstrating genetic information transfer from the mitochondrial genome to the nucleus during flowering plant evolution .
[ Sen. 2, subscore: 1.00 ]: The sequence s11-1 encodes an N-terminal extension as well as an organelle-derived RPS11 region .
[ Sen. 4, subscore: 1.00 ]: The deduced N-terminal region of s11-2 , a second nuclear-encoded homolog of rps11 , shows high sequence similarity with the putative presequence of cytochrome oxidase subunit Vb .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 2.00
Title: Molecular analysis of six segments of Tobacco leaf enation virus , a novel phytoreovirus from tobacco
Author: Picton A Potgieter C Rey ME .
Journal: Citation: V : ( ) P : Year: 2007 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub17356907 Accession (PMID): 17356907
Abstract: Tobacco leaf enation virus ( TLEV ) is a putative member of the genus Phytoreovirus within the family Reoviridae . Previous western blot analysis of structural viral proteins ( apparent molecular weights of 93 kDa ; 58 kDa ; 48 kDa ; 39 kDa and 36 kDa ) associated with TLEV , isolated from infected tobacco in South Africa , suggested that these proteins may correspond to structural Wound tumor virus ( WTV ) proteins . To further establish the nature of this novel virus disease phenotype in tobacco , molecular characterization of six dsRNA components was undertaken . Full-length cDNA clones were obtained by an optimized modified single-primer amplification sequence-independent dsRNA cloning method . Results of this study revealed the conserved terminal sequence : 5GG ( U/C ) . . . UGAU 3 of segments S6-S12 , while adjacent to these conserved terminal sequences are imperfect inverted repeats ( 7-15 bp in length ) , both features being common to reoviruses . The complete nucleotide sequences of segments S5 ( 2 , 610 bp ) , S7 ( 1 , 740 bp ) , S8 ( 1 , 439 bp ) , S10 ( 1 , 252 bp ) , S11 ( 1 , 187 bp ) and S12 ( 836 bp ) were determined . Comparison of full-length nucleotide sequences with corresponding segments of other phytoreoviruses , Rice gall dwarf virus ( RGDV ) , Rice dwarf virus ( RDV ) and WTV has shown nucleotide and predicted amino acid identities within the range of 30-60% . TLEV consistently shows a higher identity to WTV than to other phytoreovirus species where sequence data is available . Each segment had a single predicted open reading frame encoding proteins with calculated molecular weights of S5 ( 90 . 6 kDa ) ; S7 ( 58 . 1 kDa ) ; S8 ( 47 . 7 kDa ) ; S10 ( 39 . 8 kDa ) ; S11 ( 35 kDa ) and S12 ( 19 . 5 kDa ) . The relatively low nucleotide and amino acid identity to other members of the genus demonstrates that TLEV is a novel phytoreovirus , distinct from the only other reported dicotyledenous-infecting WTV and is the first phytoreovirus reported to emerge in Africa .
Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: The complete nucleotide sequences of segments S5 ( 2 , 610 bp ) , S7 ( 1 , 740 bp ) , S8 ( 1 , 439 bp ) , S10 ( 1 , 252 bp ) , S11 ( 1 , 187 bp ) and S12 ( 836 bp ) were determined .
[ Sen. 9, subscore: 1.00 ]: Each segment had a single predicted open reading frame encoding proteins with calculated molecular weights of S5 ( 90 . 6 kDa ) ; S7 ( 58 . 1 kDa ) ; S8 ( 47 . 7 kDa ) ; S10 ( 39 . 8 kDa ) ; S11 ( 35 kDa ) and S12 ( 19 . 5 kDa ) .
Supplemental links/files: reference in endnote online text related articles pubmed citation
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